Labshake search
Citations for New England Biolabs :
951 - 1000 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2022Quote: ... PCR reactions were performed using NEBNext® High-Fidelity 2X PCR Master Mix (New England BioLabs, catalog number M0541) following the manufacture’s protocol unless specified differently ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were eluted in 20 μL of elution buffer and PCR-amplified using the NEBNext 2X Master Mix (NEB) for 11 cycles and sequenced on a NextSeq 500 (Illumina) ...
-
bioRxiv - Microbiology 2020Quote: ... Amplification was performed on 4 ng of pKD3 plasmid using Q5 High-Fidelity 2X Master Mix (New England Biolabs). The PCR product was digested for 1 hour with the restriction enzymes DpnI and ClaI at 37°C and then the PCR product was run on a 1% agarose gel ...
-
bioRxiv - Microbiology 2021Quote: ... the inserts and the plasmids were mixed with Gibson master mix 2x (100 µL 5X ISO Buffer, 0.2 µL 10,000 U/mL T5 exonuclease (NEB #M0363S), 6.25 µL 2,000 U/mL Phusion HF polymerase (NEB #M0530S) ...
-
bioRxiv - Bioengineering 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase or Q5 High-Fidelity 2X Master mix (New England Biolabs). PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were amplified using a one-step barcoding PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB, M0541L) and the following primers:
-
bioRxiv - Molecular Biology 2019Quote: ... then amplified and indexed with unique library indices using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs). PCR products were purified with AMPure XP beads (Beckman-Coulter ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... For molecular cloning or any other application requiring high sequence fidelity Q5 High Fidelity 2X Master Mix (NEB, M0492S) was used ...
-
bioRxiv - Microbiology 2020Quote: ... Individual gBlocks fragment and a NdeI-HindIII-digested pET21b backbone were asssembled using a 2x Gibson master mix (NEB). Gibson assembly was possible due to a 23-bp sequence shared between the NdeI-HindIII-cut pET21b backbone and the gBlocks fragment ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The oligo was PCR amplified with OneTaq® 2X Master Mix with Standard Buffer (New England BioLabs Inc., M0482L), followed by agarose gel purification with GenCatch Advanced Gel Extraction Kit (Epoch Life Science ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 11.25 μ L OneTaq Hot Start 2X Master Mix with Standard Buffer (Catalog No. M0484, New England BioLabs Inc.), 1.25 μ L Chai Green Dye 20X (Catalog No ...
-
bioRxiv - Cancer Biology 2020Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was cleaned twice with (0.9X ...
-
bioRxiv - Cancer Biology 2021Quote: ... Libraries were generated by 7 rounds of PCR amplification using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR was used to amplify the targeted allele (Quick-Load® Taq 2X Master Mix, New England Biolabs Inc), using the following primers (at 0.4μM) ...
-
bioRxiv - Immunology 2021Quote: ... Point mutations were introduced with a QuikChange site-directed mutagenesis protocol using Q5 High-Fidelity 2x Master Mix (NEB). NLRP3 mutants were named ...
-
bioRxiv - Cell Biology 2020Quote: ... Integration at the correct locus was verified by colony PCR using LongAmp Taq 2X Master Mix (New England BioLabs), and the mto2 gene was sequenced in its entirety to verify the S338N or S338C point mutations.
-
bioRxiv - Immunology 2021Quote: All PCR reactions were carried out in 25 μL total reaction volumes using 2X Master Mix (New England Biolabs), oligonucleotide primers synthesized by Eurofins (Ebersberg ...
-
bioRxiv - Cell Biology 2021Quote: ... The cDNAs encoding human elongation factor eEF2 were amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB) with primers (forward primer ...
-
bioRxiv - Molecular Biology 2022Quote: ... and a PCR with 10-13 cycles was performed using the NEBNext High Fidelity 2X PCR Master Mix (NEB) and Ad1_noMX and Ad2.1–2.12 barcoded primers described in (30) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Purified HTO sequencing libraries were then amplified by PCR (1μl clean HTO cDNA, 25μl 2X NEBNext Master Mix (NEB #M0541), 10 µM SI-PCR and D701or D704 primers performed dial out PCR (98°C (10 sec) ...
-
bioRxiv - Biochemistry 2022Quote: ... and coding sequences were amplified and assembled into linearized pDest17 using NEBuilder Hifi Assembly 2x master mix (NEB, E2621).
-
bioRxiv - Genomics 2022Quote: ... We then amplified the barcodes from each clone using Q5 High Fidelity 2X Master Mix (M0492, New England Biolabs) with primers specific to our reporter gene (CAS P58-59) ...
-
bioRxiv - Genomics 2022Quote: ... Each PCR was run using 12.5μL Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA), 2.5μL template DNA ...
-
bioRxiv - Cancer Biology 2024Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was purified and size selected twice with AMPure beads (Beckman).
-
bioRxiv - Cancer Biology 2024Quote: ... One genotyping PCR sample contained 10 µL OneTaq® Quick-Load® 2X Master Mix (M0486L, New England Biolabs), 1 µL 10 µM forward primer ...
-
bioRxiv - Bioengineering 2024Quote: ... Samples were amplified using inner primers ACL-P1060 and ACL-P1061 in a 40μl PCR reaction using Q5 Hot Start 2x Master Mix (New England Biolabs) for 20-25 cycles at an annealing temperature of 64°C ...
-
bioRxiv - Genomics 2024Quote: ... CD7 or PDCD1) were amplified from T cell genomic DNA using 2X Phusion Hot Start Flex Master Mix (NEB) (primers described in Supplementary Table 3 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 2.5 μl 25μl p7 custom primer (Buenrostro et al. 2013) and 25 μl NEBNext Ultra II Q5 2x Master Mix (NEB) and a PCR with 10 cycles was conducted as stated in the Omni-ATAC protocol ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Single colonies were selected for colony PCR with Taq polymerase (Taq 2X Master Mix, NEB, Ipswich, MA, Cat#M0270L), then sent for sequencing for final confirmation ...
-
bioRxiv - Synthetic Biology 2023Quote: ... individual colonies were picked and a colony PCR using Quick-Load Taq 2X Master Mix (New England Biolabs, M0271L) was used to confirm the expected insert size ...
-
bioRxiv - Genomics 2023Quote: ... ATAC-seq libraries were prepared by ∼8 cycles of PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB) and primers containing Illumina barcodes (Buenrostro et al ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 μl of 0.1∼1 μg genomic DNA was mixed with 10 μl of 2X C-circle master mix [0.2 mg/ml BSA (NEB), 0.2% Tween-20 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and PCR amplified using Q5® Hot Start High-Fidelity 2X Master Mix (New England BioLabs; Ipswich, MA, USA). SafeSeqS(52 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...
-
bioRxiv - Genomics 2022Quote: ... 5ul (or at least 60ng) of cDNA was mixed with 2x Q5 HF PCR Master Mix (New England Biolabs) and 500nM of P5/R1-par and P7/SI-R2 primers in a 50ul reaction volume and subjected to the following PCR program ...
-
bioRxiv - Cell Biology 2023Quote: ... The plasmid inserts were amplified by PCR using the Q5® Hot-Start High-Fidelity 2X Master Mix (NEB). Site-directed mutagenesis was performed using the Q5® Site-directed mutagenesis kit (NEB ...
-
bioRxiv - Immunology 2023Quote: ... and second-strand cDNAs were synthesised using the LongAmp Taq 2X Master Mix (New England Biolabs, M0287L, Ipswich, USA). The double-stranded cDNAs were end-repaired using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 µL i7 unique index primer (10 µM) and 25 µL NEBNext High-Fidelity 2X PCR Master Mix (NEB) and subjected to the following PCR program ...
-
bioRxiv - Microbiology 2023Quote: ... This was followed by PCR using One Taq Quick-Load 2x Master Mix with Standard Buffer from BioLabs (UK).
-
bioRxiv - Molecular Biology 2023Quote: LAMP assays were developed with the Warm Start Colorimetric LAMP 2X Master Mix (DNA & RNA) (New England Biolabs, USA), using a mixture of LAMP oligonucleotides at a final concentration of 1.6 μM FIP and BIP ...
-
bioRxiv - Cancer Biology 2023Quote: ... The reactions were performed in triplicate for 14 cycles using Q5 Hot Start High-Fidelity 2X Master Mix (NEB), pooled and purified using AMPure XP beads (Beckman Coulter).
-
bioRxiv - Neuroscience 2023Quote: ... NaV1.6A or NaV1.6N using PCR mutagenesis with Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) as described previously (22) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 2 μL i7 unique index primer (10 μM) and 25 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB) were added to 21 μl of purified CUT&TAG DNA ...
-
bioRxiv - Microbiology 2023Quote: ... Gibson assemblies were completed using a 2x HI-FI Assembly master mix following the manufacturer’s protocol (New England Biolabs). PCR clean-up was done using the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... 16 reactions were carried out per amplicon per cell line using Q5 High-Fidelity 2x master mix (NEB, #M0492L) with primers containing partial Illumina adapters ...
-
bioRxiv - Cancer Biology 2023Quote: ... Two pairs of primers were used to perform PCR amplification with Q5 High-Fidelity 2X Master Mix (NEB, #M0492S). All validation primers are listed in Supplementary Table 3 ...
-
Understanding Campylobacter coli isolates from the Vietnamese meat production network; a pilot studybioRxiv - Microbiology 2023Quote: ... manufacturer recommended PCR conditions were used with eight minutes amplification using NEB LongAmp Taq 2X Master Mix (NEB M0287). DNA end repair was performed using NEBNext Ultra II End repair/dA-tailing Module (E7546) ...
-
bioRxiv - Biochemistry 2023Quote: ... Snap-cooled RNAs were diluted to 8 nM in 2x RNA master mix (0.2 mg/mL bovine serum albumin (molecular biology grade, NEB), 0.2 mg/mL yeast tRNA ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Unmethylated linear DNA was generated by PCR using NEB’s Q5 HotStart High-Fidelity 2x Master Mix (NEB CN# M0494S) from plasmid extracted from E ...
-
bioRxiv - Genomics 2024Quote: ... cDNA was PCR amplified using the NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Cat. No. M0541) with the following forward and reverse primers ...