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Citations for New England Biolabs :
901 - 950 of 4746 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...
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bioRxiv - Genomics 2022Quote: ... 5ul (or at least 60ng) of cDNA was mixed with 2x Q5 HF PCR Master Mix (New England Biolabs) and 500nM of P5/R1-par and P7/SI-R2 primers in a 50ul reaction volume and subjected to the following PCR program ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid inserts were amplified by PCR using the Q5® Hot-Start High-Fidelity 2X Master Mix (NEB). Site-directed mutagenesis was performed using the Q5® Site-directed mutagenesis kit (NEB ...
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bioRxiv - Immunology 2023Quote: ... and second-strand cDNAs were synthesised using the LongAmp Taq 2X Master Mix (New England Biolabs, M0287L, Ipswich, USA). The double-stranded cDNAs were end-repaired using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µL i7 unique index primer (10 µM) and 25 µL NEBNext High-Fidelity 2X PCR Master Mix (NEB) and subjected to the following PCR program ...
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bioRxiv - Microbiology 2023Quote: ... This was followed by PCR using One Taq Quick-Load 2x Master Mix with Standard Buffer from BioLabs (UK).
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bioRxiv - Molecular Biology 2023Quote: LAMP assays were developed with the Warm Start Colorimetric LAMP 2X Master Mix (DNA & RNA) (New England Biolabs, USA), using a mixture of LAMP oligonucleotides at a final concentration of 1.6 μM FIP and BIP ...
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Understanding Campylobacter coli isolates from the Vietnamese meat production network; a pilot studybioRxiv - Microbiology 2023Quote: ... manufacturer recommended PCR conditions were used with eight minutes amplification using NEB LongAmp Taq 2X Master Mix (NEB M0287). DNA end repair was performed using NEBNext Ultra II End repair/dA-tailing Module (E7546) ...
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bioRxiv - Cancer Biology 2023Quote: ... The reactions were performed in triplicate for 14 cycles using Q5 Hot Start High-Fidelity 2X Master Mix (NEB), pooled and purified using AMPure XP beads (Beckman Coulter).
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bioRxiv - Synthetic Biology 2023Quote: ... Single colonies were selected for colony PCR with Taq polymerase (Taq 2X Master Mix, NEB, Ipswich, MA, Cat#M0270L), then sent for sequencing for final confirmation ...
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bioRxiv - Synthetic Biology 2023Quote: ... individual colonies were picked and a colony PCR using Quick-Load Taq 2X Master Mix (New England Biolabs, M0271L) was used to confirm the expected insert size ...
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bioRxiv - Neuroscience 2023Quote: ... NaV1.6A or NaV1.6N using PCR mutagenesis with Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs) as described previously (22) ...
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bioRxiv - Genomics 2023Quote: ... ATAC-seq libraries were prepared by ∼8 cycles of PCR using NEBNext High Fidelity 2X PCR Master Mix (NEB) and primers containing Illumina barcodes (Buenrostro et al ...
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bioRxiv - Microbiology 2023Quote: ... Gibson assemblies were completed using a 2x HI-FI Assembly master mix following the manufacturer’s protocol (New England Biolabs). PCR clean-up was done using the GeneJET PCR Purification Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Two pairs of primers were used to perform PCR amplification with Q5 High-Fidelity 2X Master Mix (NEB, #M0492S). All validation primers are listed in Supplementary Table 3 ...
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bioRxiv - Biochemistry 2023Quote: ... OSCA1.2 pIRES2-mCherry vector and the BLD of OSCA3.1 were separately amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB), followed by fragment assembly using Gibson Assembly Master Mix (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... 16 reactions were carried out per amplicon per cell line using Q5 High-Fidelity 2x master mix (NEB, #M0492L) with primers containing partial Illumina adapters ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 2.5 μl 25μl p7 custom primer (Buenrostro et al. 2013) and 25 μl NEBNext Ultra II Q5 2x Master Mix (NEB) and a PCR with 10 cycles was conducted as stated in the Omni-ATAC protocol ...
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bioRxiv - Genomics 2024Quote: ... CD7 or PDCD1) were amplified from T cell genomic DNA using 2X Phusion Hot Start Flex Master Mix (NEB) (primers described in Supplementary Table 3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Unmethylated linear DNA was generated by PCR using NEB’s Q5 HotStart High-Fidelity 2x Master Mix (NEB CN# M0494S) from plasmid extracted from E ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μL i7 unique index primer (10 μM) and 25 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB) were added to 21 μl of purified CUT&TAG DNA ...
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bioRxiv - Biochemistry 2023Quote: ... Snap-cooled RNAs were diluted to 8 nM in 2x RNA master mix (0.2 mg/mL bovine serum albumin (molecular biology grade, NEB), 0.2 mg/mL yeast tRNA ...
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bioRxiv - Cancer Biology 2024Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was purified and size selected twice with AMPure beads (Beckman).
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bioRxiv - Cancer Biology 2024Quote: ... One genotyping PCR sample contained 10 µL OneTaq® Quick-Load® 2X Master Mix (M0486L, New England Biolabs), 1 µL 10 µM forward primer ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were amplified using inner primers ACL-P1060 and ACL-P1061 in a 40μl PCR reaction using Q5 Hot Start 2x Master Mix (New England Biolabs) for 20-25 cycles at an annealing temperature of 64°C ...
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bioRxiv - Genomics 2023Quote: ... BAC baits were PCR amplified using 5’ biotinylated forward and reverse primers (Supplementary Table S7) in the following reaction mix: 10 µl 2X NEBNext® Ultra™ II Q5® Master Mix (NEB cat. M0544S), 1 µl 10 µM each bio-primer ...
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bioRxiv - Cell Biology 2021Quote: ... two-step RT-qPCR was performed using LunaScript® RT SuperMix Kit (E3010L) for cDNA synthesis and Luna® Universal qPCR Master Mix (NEB #M3003) for RT-qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... real time PCR was performed in triplicate using Luna® Universal qPCR Master Mix (New England Biolabs) with variable amounts of cDNA and 0.5 μM of target-specific primers in a CFX connect instrument (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Multiplex quantitative PCR was then performed using the Luna Universal Probe qPCR Master Mix (ref M3004, NEB), a Roche LightCycler or Rotor Gene Q real-time PCR (Qiagen ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA was used as a template for quantitative PCR using Luna Universal qPCR Master Mix (NEB) and a Viia7 real-time PCR instrument (Life Technologies/ABI) ...
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bioRxiv - Cancer Biology 2022Quote: ... using QuantStudio™ Design & Analysis Software (v1.5.1) following Luna® Universal qPCR Master Mix (New England Biolabs) protocol and amplification parameters ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 μM reverse primer and 5 μL Luna® Universal Probe qPCR Master Mix (New England Biolabs)] amplified using the Prism7900HT sequence detection system (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... including 2μL of gDNA and 10μL Luna® Universal Probe qPCR Master Mix (New England Biolabs, Australia). Final concentrations of the forward (S0947 ...
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bioRxiv - Physiology 2023Quote: ... thermocycler with the Luna Universal one-step qPCR Master Mix (E3005; New England Biolabs, Ispwich, MA, USA). Amplification reactions for genes of interest were performed in 50 cycles of the following cycling protocol ...
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bioRxiv - Genomics 2023Quote: ... Real-time polymerase chain reaction (PCR) was performed using Luna® Universal qPCR Master Mix (NEB M3003L) and the Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1845097) ...
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bioRxiv - Microbiology 2023Quote: ... using the kit Luna® Universal qPCR Master Mix Kit (New England Biolabs, Ipswich, MA, United States). PCRs were conducted in a total volume of 20 µl containing 10 μL of Luna Universal qPCR Mix ...
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bioRxiv - Molecular Biology 2023Quote: ... 5mL of each cDNA sample was added to a Luna® Universal Probe qPCR Master Mix (NEB). 250nM of primers and probes were added to master mix ...
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bioRxiv - Molecular Biology 2022Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the relative amount of origin DNA compared to the terminus ...
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bioRxiv - Molecular Biology 2022Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the amount of genomic loci bound to DnaA ...
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bioRxiv - Molecular Biology 2022Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the relative amount of origin DNA compared to the terminus ...
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bioRxiv - Molecular Biology 2022Quote: qPCR was performed using the Luna qPCR mix (NEB) to measure the amount of genomic loci bound to DnaA ...
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bioRxiv - Microbiology 2020Quote: ... Illumina sample-specific barcodes and sequencing adapters were added in a final PCR reaction consisting of (per sample) 10 μl 2x Hot Start Taq master mix (New England BioLabs), 2 μl each of Nextera N7 and S5 barcoding primers (Illumina) ...
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bioRxiv - Cancer Biology 2021Quote: ... A region around an A-to-I editing site in BPNT1 was amplified by Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs) and the primers ...
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bioRxiv - Cell Biology 2020Quote: ... Quantification of mRNA level was performed in a 20 μl mixture consisting of 10 μl Q5 High-Fidelity 2X Master Mix (NEB), 0.2 μl RT-PCR product ...
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bioRxiv - Cell Biology 2020Quote: ... 1μl of each lysate was used as a PCR template to amplify 600-800 bp fragments around the edited E-box using the Q5 High-Fidelity 2X Master Mix (NEB). The resulting PCR products were purified in 96-well plates using the AmpliClean DNA Cleanup Kit (Nimagen ...
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bioRxiv - Cell Biology 2020Quote: ... R1/R2 purified fragments were split in 12× 25 μl-reaction PCR reactions with Q5 High-Fidelity 2X Master Mix (New England Biolabs), and NEBNext® Multiplex Oligos for Illumina® ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR amplification of the N-terminal region of TRIM1 was performed using Q5 High-Fidelity 2X Master Mix (New England BioLabs) with the primers ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were amplified for 12 PCR cycles with unique dual index primers using the NEBNext Hi-Fi 2X PCR Master Mix (New England Biolabs). Amplified libraries were purified using a 1.2X ratio of Axygen magnetic beads (Corning Inc ...
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bioRxiv - Physiology 2022Quote: ... All mutations were carried out using Q5 High-Fidelity 2X Master Mix from New England Biolabs (NEB, Ottawa, ON, Canada) and verified by sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... and PCR amplified for 13 cycles with Illumina Nextera adapter primers using the NEBNext High Fidelity 2X Master Mix (NEB) with the following PCR program ...