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Citations for New England Biolabs :
8801 - 8850 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The HeLa core histones in the kit were replaced with equal amounts of recombinant histones from NEB (Cat#: M2508S and M2509S) for recombinant histone samples ...
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bioRxiv - Bioengineering 2024Quote: ... PCR was performed with KOD Multi & Epi PCR kit (TOYOBO) and Gibson Assembly was performed with Gibson Assembly Master Mix (NEB, E2611).
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Foxn3 is part of a transcriptional network that regulates cilia genes in the developing mouse retinabioRxiv - Developmental Biology 2024Quote: ... A total of 500 ng of RNA was used to prepare the RNAseq library using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina and NEB Unique Dual Index UMI Adaptors (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed by the OIST Sequencing section using the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England BioLabs, USA). Sequencing was performed using an Illumina NovaSeq6000 platform at OIST.
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bioRxiv - Genomics 2024Quote: ... 100µg/ml) containing E.coli transfected with the plasmids were processed using the Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010). Subsequently ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of pooled TEDseq libraries was subjected to end repair and A-tailing using the NEBNext® FFPE DNA Library Prep Kit (NEB:E6650S). Nanopore adapter ligation and library construction was conducted using the Nanopore Ligation Sequencing Kit (ONT ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP–seq libraries were prepared from 3–5 ng of ChIPed DNA using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645S), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The fragment was subcloned into EcoRI-cut pENTR2b plasmid using a NEBuilderHiFi Gibson Assembly Kit (New England Biolabs, Ipswich, MA, USA). The Gateway destination vector pMpGWB403 (Ishizaki et al ...
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bioRxiv - Bioengineering 2024Quote: All mRNAs were generated via in vitro transcription (IVT) reactions using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs E2040S). Coding sequences were ordered as gBlocks (IDT ...
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bioRxiv - Genomics 2024Quote: ... The samples were PCR amplified for 15 cycles using compatible PCR Barcodes from the kit and LongAmp Taq 2X master mix (NEB, US). The amplified product was purified ...
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bioRxiv - Genomics 2024Quote: ... Zirconium beads (3.0mm) in a bead beater were used to homogenize tissue before proceeding with NEB Monarch Total RNA Miniprep Kit (NEB #T2010), following manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: We created resequencing libraries for each sample using a modified half reaction protocol for the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc.), with a fragmentation time of five minutes ...
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bioRxiv - Genetics 2024Quote: ... Total RNA input was normalized and NGS libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs, E6420S) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA libraries were constructed utilizing the NEBNext Ultra II Directional RNA Library Prep (stranded) kit with sample purification beads (NEB, #E7765L). cDNA libraries were sequenced on the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Genomics 2024Quote: ... approximately 1 μg of total RNA underwent rRNA depletion using the NEBNext rRNA Depletion Kit (NEB, Ipswich, MA, USA, Cat# E6318). RNA-seq libraries were then prepared following the whole transcriptome sequencing protocol of Novogene (https://www.novogene.com/us-en/resources/blog/a-basic-guide-to-rna-sequencing/) ...
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bioRxiv - Genomics 2024Quote: ... and approximately 60 million cells per sample were carried forward for UHMW DNA extraction using the NEB Monarch Cells & Blood DNA extraction kit (New England Biolabs, T3050), with modifications as recommended by the manufacturer for ONT Ultra long sequencing application ...
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bioRxiv - Neuroscience 2024Quote: ... 5’UTR - GluA1flip(R) - 3’UTR and GluA3 flip(Q) (Y454A/R461G) was obtained using the Q5® Site-Directed Mutagenesis Kit (NEB). To obtain Halo-tag - GluA2flip(Q ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA extraction from HCT116 WT and DKO cells were performed with Monarch genomic DNA purification kit (NEB, catalog number: T3010), following the protocol for genomic DNA extraction from cells ...
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bioRxiv - Biochemistry 2024Quote: ... and K51A) were generated from the pTBSG WT Ig3 construct using the Q5® site-directed mutagenesis kit (New England Biolabs). Sequences were verified by DNA sequencing (Supplemental Note 1 provides each protein sequence) ...
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bioRxiv - Bioengineering 2024Quote: ... tail were linearized with SapI or XbaI and transcribed using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (New England Biolabs, E2080). In vitro transcription was performed with complete substitution of uridine with N1-methylpseudouridine (Trilink Biotechnologies ...
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bioRxiv - Cancer Biology 2024Quote: ... This modified vector was digested at 37ºC for 1 hour and purified with Monarch® DNA Gel Extraction Kit (NEB #T1020).
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bioRxiv - Biophysics 2024Quote: ... A BsmI site was introduced into the plasmid by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: The iDAb LMO2-E3 ligases fusions were generated by polymerase chain reaction (PCR) using Phusion® High-Fidelity PCR kit (NEB). pEF-VH576-L10-FLAG-CRBN-mb was used as a template to generate VH576-L10-FLAG-CRBN ...
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bioRxiv - Cell Biology 2024Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 ng of total RNA were used to generate barcoded RNA-Seq libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs Inc). Libraries were sequenced on a NextSeq 2000 (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... Library preparation was performed using Nebnext Ultra II directional RNA library prep kit for Illumina with Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... pSEMS-HKI-N-Halo encoding the first 17 residues of rat HKI fused to a HaloTag was created by fusion PCR using NEBuilder HiFi DNA assembly kit (New England Biolabs, E5520) and the amplified DNA fragment inserted via EcoRI and XhoI sites into expression vector pSEMS-Halo (Covalys Biosciences) ...
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bioRxiv - Bioengineering 2024Quote: ... Additional mutations were cloned into SpCas9-VRQR nuclease or ABE plasmids using isothermal assembly the Q5 Site-Directed Mutagenesis Kit (E0554; New England Biolabs; NEB). Expression plasmids for human U6 promoter-driven gRNAs were generated by annealing and ligating duplexed oligonucleotides corresponding to spacer sequences into BsmBI-digested pUC19-U6-BsmBI_cassette-SpCas9_gRNA (BPK1520 ...
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bioRxiv - Bioengineering 2024Quote: A poly-adenylated transcript encoding mGL was in-vitro transcribed using the HiScribe T7 mRNA Kit with CleanCap Reagent AG (NEB, E2080S). The transcript contains a genetically encoded 67 nt poly(A ...
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bioRxiv - Cell Biology 2024Quote: ... and S1+S2) and ciliopathy patient variants (F343V, K329T, and L349S) were generated using the Q5 Site-Directed Mutagenesis Kit (NEB; E0554S) utilizing the full-length ...
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bioRxiv - Cell Biology 2024Quote: ... Constructs encoding fragments of the CATSPERε extracellular domains (ECDs) were subcloned into phCMV3 vector using NEBuilder® HiFi DNA Assembly Kit (NEB).
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using 200 ng of genomic DNA following the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs, E7805S). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ChIP-seq libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S). Size selection was carried out in samples with > 50 ng of total DNA ...
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bioRxiv - Bioengineering 2024Quote: ... Additional mutations were cloned into BE plasmids using isothermal assembly the Q5 Site-Directed Mutagenesis Kit (E0554; New England Biolabs; NEB). Expression plasmids for human U6 promoter-driven gRNAs were generated by annealing and ligating duplexed oligonucleotides corresponding to spacer sequences into BsmBI-digested pUC19-U6-BsmBI_cassette-SpCas9_gRNA (BPK1520 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg of RNA was converted to cDNA using the ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to reverse transcribe poly-adenylated mRNA into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs) as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... The enriched DNA was used to construct sequencing libraries with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7103). Libraries were sequenced (2 x 50bp paired end ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were end-repaired and adaptor-ligated using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, cat. no. E7645S), and indexed in duplicate by PCR using the Herculase II Fusion DNA Polymerases kit (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA-seq library was prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) after ribosomal RNA (rRNA ...
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bioRxiv - Cell Biology 2024Quote: ... Following in vitro transcription at 37°C overnight using the HiScribeTM T7 High Yield RNA Synthesis Kit (New England Biolabs (NEB)) ...
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bioRxiv - Developmental Biology 2024Quote: ... The generation or origin of the plasmids is described in Table S1 43–45 and for the assembly reactions we used the NEBuilder HiFi Assembly cloning kit (NEB E5520S). PCR reactions were carried out using the Q5 High-Fidelity DNA polymerase (NEB MO515 ...
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bioRxiv - Genetics 2024Quote: ... genomic DNA was extracted from approximately 6 million COLO320-DM cells using the Monarch HMW DNA Extraction Kit for Tissue (NEB #T3060L) following the Oxford Nanopore Ultra-Long DNA Sequencing Kit V14 protocol ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared by the University of Michigan Advanced Genomics Core (AGC) using the NEBNext Ultra II FS DNA Library Prep Kit (NEB, #E7805) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Translations in the PURE system (Protein synthesis Using Recombinant Elements) were performed using the PURExpress Δ(aa,tRNA) Kit (New England Biolabs). Translation reactions were assembled with 10 ng/µL of the appropriate plasmid DNA ...
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bioRxiv - Cell Biology 2024Quote: ... The purified product was used as a template for T7 in vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). In vitro transcribed sgRNAs were DNAse-treated ...
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bioRxiv - Evolutionary Biology 2024Quote: ... according to the manufacturer’s directions using a 5-fold dilution of the universal adaptor and 9 cycles of PCR per the respective masses with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760L), the NEBNext Poly(A ...
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bioRxiv - Genetics 2024Quote: ... Plasmids expressing sgRNA for Base Editor-dCas9 mediated editing were generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs), using the following primers ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and NGS libraries after size selection and a DNA cleanup step (using Monarch PCR & DNA cleanup kit; T1030S, New England BioLabs Inc.) were analysed with Bioanalyzer (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2024Quote: ... CCAP-treated RNA was used for preparation of small RNA next- generation sequencing libraries with NEBNext Multiplex Small RNA Library Prep Kit (E7560S, New England BioLabs Inc.) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Whole-genome data for these latter strains was obtained by extracting genomic DNA from 6 pooled adult aphids using a Monarch® Genomic DNA Purification Kit (New England BioLabs), one pool per strain ...