Labshake search
Citations for New England Biolabs :
8751 - 8800 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: The pET15b-CtTrl1-LIG plasmid22 served as template for PCR-based site direct mutagenesis with one oligonucleotide for K148N and E328-stop (ΔCTD) variants or two oligonucleotides and Q5® Site-Directed Mutagenesis Kit (NEB) to introduce R334A ...
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bioRxiv - Cell Biology 2024Quote: ... Site directed mutagenesis was performed according to the manufacturer’s instructions to introduce gRNA target-site mutations in wild-type Pcyt2 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554). Primers were designed using NEBaseChangerv1.
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bioRxiv - Biochemistry 2024Quote: Active site mutations of PqqU were generated from the plasmid template pBAD24-pqqU using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Plasmids encoding TALE-free MTS–G1397-split DddAtox/DddA6/DddA11–UGI were generated with the Q5® Site-Directed Mutagenesis (SDM) Kit (NEB), using the corresponding herein developed backbone plasmids as templates for each final construct containing either the DddAtox ...
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bioRxiv - Synthetic Biology 2024Quote: ... Reaction mixtures were treated with DNase I and incubated at 37 °C for 30 minutes before immediate purification (Monarch® RNA Cleanup Kit, NEB). A sample of each mRNA was reserved for quality analysis and transcripts were tailed with E ...
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bioRxiv - Immunology 2024Quote: ... RNA aliquots were used for library preparation using NEBNext Multiplex Small RNA library preparation kit (New England Biolabs, Ipswich, MA, USA). The PCR amplified cDNA construct (from 140–160 bp ...
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bioRxiv - Microbiology 2024Quote: Library preparation and sequencing were conducted using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina with dual index primers and ∼300 ng of DNA (NEB Inc.) on an automation platform (Biomeki5 ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to identify 5-methylcytosine and 5-hydroxymethylcytosine bases and sequencing libraries were constructed by NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, #E7645S), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Riboprobe for the full-length ACT1 gene was made using “HiScribe™ T7 Quick High Yield RNA Synthesis Kit” (NEB, USA) using Fluorescein labelling mix (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2024Quote: Transcription templates for expressing gRNA variants and SARS-CoV-2 or CMV input RNA fragments were generated by PCR (Phusion high-fidelity PCR kit, NEB #E0553) of the gBlock or Ultramer template that included a T7 promoter and the gRNA or input RNA coding sequence ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA samples were converted to sequencing libraries using the NEBNext Small RNA Library Prep kit for Illumina (New England Biolabs, E7300) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: DICER RNA substrates have been obtained using PCR synthetized linear DNA bearing T7 promoter and HiScribe T7 High Yield RNA Synthesis Kit (NEB #E2040S) following manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Length of DNA Fragments were determined by agarose gel electrophoresis and then purified with Monarch DNA Gel Extraction Kit (NEB # T1020L). DNA concentration has been assessed by Nanodrop.
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bioRxiv - Microbiology 2024Quote: ... was digested with XbaI and MluI restriction enzymes and barcoded linear HA libraries were inserted into the lentivirus backbone using NEB HiFi assembly kit (NEB E5520S). Assembled plasmid libraries were then electroporated into electrocompetent 10b cells (NEB C3020K ...
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bioRxiv - Neuroscience 2024Quote: ... Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB E7760L). Purified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (5067-5582 and 5067-5583) ...
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bioRxiv - Bioengineering 2024Quote: Samples were resuspended in 5 µl 1X NEBNext Cell Lysis Buffer of the NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB, E6420). After 5 min incubation at RT ...
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bioRxiv - Plant Biology 2024Quote: ... the RNA samples were reverse transcribed using the First-Strand cDNA Synthesis Kit for RT-PCR (AMV) (NEB Inc. Ipswitch, MA). The cDNA samples were then diluted 1:20 before qPCR ...
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bioRxiv - Neuroscience 2024Quote: ... RNA-sequencing libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs, E6420S) after normalizing RNA input ...
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bioRxiv - Microbiology 2024Quote: ... cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs; Ipswich, MA). RNA sequencing was performed on an Illumina NovaSeq6000 system with 100x coverage.
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bioRxiv - Neuroscience 2024Quote: Total RNA samples were prepared into RNAseq libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs E7760L) following manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: Libraries for CUT&RUN samples were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, cat# E7645S) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... In brief RNA-seq libraries were prepared from total RNA using polyA selection followed by the NEBNext Ultra II Directional RNA Library Prep Kit protocol (New England Biolabs, E7760S). Sequencing was performed on Illumina HiSeq 2500 instrument resulting in approximately 30 million 50 bp reads per sample ...
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bioRxiv - Pathology 2024Quote: ... Barcoded RNA-seq library was established with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Site-directed mutagenesis was performed to generate the AeCRKG359E (inactive kinase mutation) and AeRLCK2G110E (rlck2-6 mutant allele mutation) variants using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The primers used were AeCRKkin_Mut_G359E_F and AeCRKkin_Mut_G359E_R and the AeRLCK2mutL42F and AeRLCKmutL42R ...
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bioRxiv - Systems Biology 2024Quote: ... 10 ng nuclear RNA and 100 ng whole-cell RNA was used as template for RT-qPCR using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005). PCR was performed per manufacturer recommendations ...
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bioRxiv - Systems Biology 2024Quote: ... Total RNA was extracted from each sample (+/- SIRLOIN/TSO, nuclei and whole cells, 4 samples total) with the NEB Total RNA Miniprep kit (NEB #T2010). 10 ng nuclear RNA and 100 ng whole-cell RNA was used as template for RT-qPCR using the NEB Luna Universal One-Step RT-qPCR kit (NEB #E3005) ...
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High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... Synthesized modRNA was column purified and eluted with 60 µL water using the 50 µg Monarch RNA Cleanup Kit (New England Biolabs, T2040). A small sample was nanodropped and ran on a native denaturing gel to determine modRNA concentration and verify full-length product ...
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High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... 200 ng of purified product served as template in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040), fully substituting UTP with N1-methylpseudouridine-5’-triphosphate (TriLink Biotechnologies ...
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bioRxiv - Neuroscience 2024Quote: NGS library preparation was carried out using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB #E7760) with NEBNext Poly(A ...
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bioRxiv - Cell Biology 2024Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Assembly reaction was performed at 1:2 vector: insert ratio using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs #E5520) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 200 ng RNA was used for library construction using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7760), together with the NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... and for Figure 2 using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). Input DNA was also used as a control ...
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bioRxiv - Microbiology 2024Quote: PCR amplified transformation cassettes were purified by column purification (Monarch PCR and DNA Cleanup Kit, New England Biolabs, Ipswich, MA, USA) prior the use for fungal transformation of A ...
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bioRxiv - Microbiology 2024Quote: Tax1bp1 was amplified by PCR from murine cDNA using the primers named Tax1bp1 fwd and rev (Table 2) and inserted by ligation-independent cloning (NEBuilder Builder HiFi DNA Assembly Cloning Kit, NEB #E5520S) into pENTR1A no ccdB (w48-1 ...
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bioRxiv - Systems Biology 2024Quote: ... The purified DNA was used to generate next-generation sequencing libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645S) and NEBNext Multiplex Oligos for Illumina Index Primers Set 1 and 3 (NEB E7335S ...
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bioRxiv - Physiology 2024Quote: ... cDNA was obtained by reverse transcription of total RNA using an Lunascript® RT Supermix cDNA synthesis kit (New England Biolabs). cDNA products were detected using an iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... ribosomal RNA (rRNA) depletion was performed using NEBNext rRNA depletion kit v2 (Human/Mouse/Rat) with RNA sample purification beads (NEB, #E7405X). cDNA libraries were constructed utilizing the NEBNext Ultra II Directional RNA Library Prep (stranded ...
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bioRxiv - Neuroscience 2024Quote: All plasmids were custom designed and constructed with a modified mMoClo system.44 Parts were cloned with Bsa1 adapters and assembled into expression constructs with a NEB Golden Gate Assembly Kit (NEB #E1602). Plasmids were transformed into One Shot TOP10 (Thermo Fisher C404006 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The digestion products from the pR probes were purified with the Monarch PCR & DNA Cleanup Kit (New England Biolabs, Cat# T1030L) to serve as the vector ...
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bioRxiv - Genomics 2024Quote: ... Libraries were made according to the manufacturer’s directions for the NEBNext Enzymatic Methyl-seq kit (New England Biolabs, Cat. No. E7120S) using the S220 Focused-ultrasonicator (Covaris ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed by the OIST Sequencing section using the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England BioLabs, USA). Sequencing was performed using an Illumina NovaSeq6000 platform at OIST.
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bioRxiv - Genomics 2024Quote: ... The samples were PCR amplified for 15 cycles using compatible PCR Barcodes from the kit and LongAmp Taq 2X master mix (NEB, US). The amplified product was purified ...
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bioRxiv - Plant Biology 2024Quote: ... The fragment was subcloned into EcoRI-cut pENTR2b plasmid using a NEBuilderHiFi Gibson Assembly Kit (New England Biolabs, Ipswich, MA, USA). The Gateway destination vector pMpGWB403 (Ishizaki et al ...
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bioRxiv - Bioengineering 2024Quote: All mRNAs were generated via in vitro transcription (IVT) reactions using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs E2040S). Coding sequences were ordered as gBlocks (IDT ...
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bioRxiv - Biophysics 2024Quote: ... Small deletions (Loop and Linker) in mouse cMyBP-C wildtype sequences were accomplished using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554). The following primers (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... 100µg/ml) containing E.coli transfected with the plasmids were processed using the Monarch® Plasmid DNA Miniprep Kit Protocol (NEB #T1010). Subsequently ...
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bioRxiv - Genomics 2024Quote: ... Zirconium beads (3.0mm) in a bead beater were used to homogenize tissue before proceeding with NEB Monarch Total RNA Miniprep Kit (NEB #T2010), following manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: We created resequencing libraries for each sample using a modified half reaction protocol for the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc.), with a fragmentation time of five minutes ...
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bioRxiv - Genetics 2024Quote: ... Total RNA input was normalized and NGS libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs, E6420S) according to the manufacturer’s instructions ...