Labshake search
Citations for New England Biolabs :
9051 - 9100 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... mixtures for the detection of viral RNA were made by a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs, USA) in accordance with the manufacturer’s instructions and then placed in a thermocycler (BIO-RAD CFX96™ Real-Time System ...
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bioRxiv - Microbiology 2022Quote: ... and ligated with Illumina sequencing adapters using a NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs, Ipswich MA, USA), and further PCR enriched with primers of P5 and P7 oligos ...
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bioRxiv - Cell Biology 2021Quote: ... A232E site-directed mutation in RFP-VCP was done with a Q5 site-directed mutagenesis kit (New England Biolabs Japan, Tokyo, Japan).
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bioRxiv - Cell Biology 2021Quote: ... The beads were then re-suspended in 23 μL of 10 mM Tris-Cl pH 8.0 and libraries were prepared by on-bead reactions using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB, E7645S). The beads were separated on a magnetic stand and the supernatant was discarded ...
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bioRxiv - Neuroscience 2021Quote: ... The libraries were prepared from total RNA enriched for mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Preparation Kit for Illumina (New England Biolabs, MA, USA) and sequenced on an Illumina NextSeq 500 instrument (Illumina ...
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bioRxiv - Genomics 2020Quote: ... and PCR enrichment of adapter-ligated DNA was performed for 10 cycles using NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs). Amplified libraries were purified with 0.7x SPRIselect beads and sequenced with MiSeq Reagent Kit v3 ...
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bioRxiv - Genomics 2021Quote: ... NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® according to the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Neuroscience 2021Quote: ... Strand-specific RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) after poly(A ...
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bioRxiv - Cancer Biology 2020Quote: cDNA was synthesized from 500 ng of each respective RNA sample using the ProtoScript® First Strand cDNA Synthesis Kit (New England BioLabs, E6300L) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... strand-specific RNA-seq libraries were constructed by using the NEB Next® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 3 to 15 ng of immunoprecipitated DNA were used to prepare the sequencing libraries using the NEBNext Ultra II DNA Library Prep Kit (NEB, Ref: E7645S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 4 single guide RNA templates fused to a scaffold were synthesized for each target gene using HiScribe™ SP6 RNA Synthesis Kit (New England BioLabs, E2070S). Injection mixture was prepared on ice containing 5 µM Cas9 (PNA Bio ...
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bioRxiv - Cell Biology 2021Quote: ... Purified RNA was submitted to the University of Virginia Genome Analysis and Technology Core for whole transcriptome sequencing by first library preparation with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB Cat #E7760) and then sequencing by Illumina NextSeq 500 Sequencing System for paired-end 75bp reads ...
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bioRxiv - Genetics 2021Quote: ... DNA libraries were prepared using 300 ng of fragmented DNA following the instructions of the NEBNext® Ultra™ II DNA Library Prep kit (New England Biolabs). Samples were sequenced on a HiSeq 2500 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared for sequencing by Cambridge Genomics Services using 3 μg of total RNA and an Ultra™ RNA library prep kit for Illumina (NEB, USA). Libraries were quantified by PCR ...
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bioRxiv - Genomics 2020Quote: ... Approximately 1 µg of fragmented DNA was used for each library preparation using the NEBNext® DNA Library Prep kit (New England Biolabs, UK); DNA-libraries were multiplexed with NEBNext Multiplex Oligos for Illumina kit (New England Biolabs ...
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bioRxiv - Genomics 2020Quote: ... and once the solution is clear 48 uL of the elution was transferred to a 2mL Lo-Bind tube with End-Repair and A-tail buffer (7 uL) and enzymes (3 uL) premixed (NEBNext® Ultra™ II kit, New England Biolabs). The solution was then mixed by gentle tapping and transferred to a 0.2 mL PCR tube and incubated at 20 degrees C for 30 minutes and 65 degrees C for 30 minutes in a thermocycler ...
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bioRxiv - Microbiology 2021Quote: ... The first EcoRI restriction site at 829 bp within the AgeI and KasI restriction sites in RGD4C-AAVP-TNF was deleted in two steps to mutate a thymidine to cytosine nucleotide at position 833 without altering the translated amino acid using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... RNA (25-100 ng) was mixed with random oligonucleotide primers using the NEBNext® First Strand Synthesis Module kit for Illumina® (NEB) and incubated for 10 min at 94°C ...
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bioRxiv - Immunology 2020Quote: ... Two hundred nanograms of total RNA was subsequently processed to generate RNA-seq libraries using NEBNext Poly(A) mRNA Magnetic Isolation and Ultra II Directional RNA Library Prep kit for Illumina (NEB E7490, E7760) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Preparation of a small RNA-Seq library from submitted total RNA sample was performed using the NEBNext® small RNA library preparation kit (New England Biolabs, UK). Small-RNA sequencing was conducted using the Illumina HiSeq 4000 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA depletion was carried on the extracted RNA using Nebnext rRNA depletion kit (Human/mouse/rat) (New England BioLabs. In, USA). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs, Ipswich, MA, USA). Purified sgRNA (0.5 μg ...
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bioRxiv - Molecular Biology 2020Quote: Primers for generating mutations in hTHAP9 and DmTNP coding sequences were designed using NEB Base changer tool™ and site directed mutagenesis was performed using the Q5 Site directed Mutagenesis kit (NEB E0554S) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: The DNA for the gene-of-interest with overlap extensions were ligated into the digested vectors using NEBuilder® HiFi DNA Assembly Cloning Kit (NEB # E5520S) by incubating at 50 °C for one hour according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The final lentiviral transfer plasmids were assembled using the NEB® Golden Gate Assembly Kit (BsmBI-v2, New England Biolabs cat E1602L).
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bioRxiv - Immunology 2021Quote: ... The concentration of libraries was determined by quantitative PCR using a NEBNext library Quant kit for Illumina (New England BioLabs, Ipswich MA). The size of libraries and amplicon profiles were determined by the Fragment Analyzer System (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... The Hi-C library for Illumina sequencing was prepped using the NEBNext® Ultra™ II DNA library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... We prepared genomic DNA libraries for each sample using the NEBNext® Ultra II DNA Library Prep Kit for Illumina® (New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 3 ng of DNA was used for library preparation according to NEBNext® Ultra™ II DNA Library Prep Kit (NEB, E7645S). Purity and size distribution was analyzed on Fragment Analyzer ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were prepared by NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA) and pair-end sequenced on the Illumina NextSeq550 System ...
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bioRxiv - Genomics 2020Quote: ... Genomic libraries were constructed and barcoded using the NEBNext Ultra DNA Library Prep kit for Illumina (New England Biolabs, Ipswich, MA, USA) and were sequenced on either the Illumina HiSeq 2500 platform (Illumina ...
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bioRxiv - Genomics 2020Quote: ... A paired-end library with insert sizes of 700 bp was performed using a NEBNext Ultra DNA library prep kit (New England BioLabs, MA, USA) and NEBNext multiplex oligos (New England BioLabs ...
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bioRxiv - Genomics 2021Quote: ... 300 ng of enriched DNA samples were fragmented using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs) according to the manufacturer’s recommendation with 5 minutes incubation time ...
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bioRxiv - Genomics 2021Quote: ... 1.5 µg of high-molecular-weight genomic DNA was subjected to end repair using the NEBNext Ultra II End Repair kit (NEB, cat. #E7445) and purified using 1x AmPure beads (Beckman Coulter Life Sciences ...
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bioRxiv - Genomics 2021Quote: WGBS single indexed libraries were generated using the NEBNext Ultra DNA library Prep kit for Illumina (New England BioLabs, Ipswich, MA, USA) on the Agilent Bravo automated liquid handling platform with a custom high-throughput protocol ...
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bioRxiv - Genomics 2021Quote: ... DNA was diluted ten-fold and used as template for the second round of PCR where Illumina adaptors for sequencing were added using the NEBNext® Multiplex Oligos Illumina dual index kit (NEB, UK) with 10 cycles of 10 seconds at 98°C ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was then extracted using phenol-chloroform16 and sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S/L). Sequencing was performed by the VUMC VANTAGE core Illumina NovaSeq (PE-150 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Whole-genome libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA) with modifications for subsequent target enrichment ...
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bioRxiv - Neuroscience 2023Quote: ... The RNA sequencing library was generated using NEBNext Ultra II Directional RNALibrary Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MS, USA). The sequencing library was validated on the Agilent TapeStation (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... The libraries for Illumina sequencing were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... End-repaired DNA was barcoded using the Native Barcoding Expansion kit (ONT, United Kingdom) and Blunt/TA Ligase Master Mix (New England Biolabs, United States). Barcoded samples were multiplexed and ligated to sequencing adaptor molecules in Adapter Mix II using the NEBNext Quick T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... gDNA from cell pellets was isolated using the Quick-DNA Midiprep Plus Kit (ZymoResearch, D4075) and genome-integrated sgRNA sequences were amplified by PCR using the Q5 Mastermix (New England Biolabs Next UltraII). i5 and i7 multiplexing barcodes were added in a second round of PCR ...
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bioRxiv - Microbiology 2022Quote: ... The sequencing library employed for total RNA sequencing was prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Cat# E7530). Paired-end 76-bp sequencing was performed using a MiSeq system (Illumina ...
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bioRxiv - Microbiology 2023Quote: HTS was performed on the three RNA extracts after a step of rRNA depletion with the rRNA depletion kit (NEB, Evry, France), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... AvcI RNA was synthesized by in vitro transcription using the T7-AvcI reverse complement DNA template and the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB™). Bio-11-UTP was included during the transcription reaction for Northern blot detection purposes ...
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bioRxiv - Molecular Biology 2023Quote: ... and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit; New England Biolabs, Ipswich, MA, USA). Specifically ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was amplified using an AMV first strand cDNA synthesis kit and was normalized to 50 ng/ul for each sample (New England Biolabs, Catalog # E6550). Sslac2 was amplified using specific detection primers (Sslac2 Det F and R ...
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bioRxiv - Physiology 2022Quote: RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... RNA sequencing library preparation used NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s recommendations (New England Biolabs, Ipswich, MA, USA). Briefly ...