Labshake search
Citations for New England Biolabs :
8951 - 9000 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Isolated mRNAs were reverse-transcribed into double stranded cDNA and subjected to sequencing library construction using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, E7770) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Site directed mutagenesis of the selectivity filter region of EhEUKCATB1 was performed using a Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Hitchin, UK). Primer sequences (SDM1_F-TGACCGGAGAcTCCTGGTCTG ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA-seq libraries were prepared using 1 µg of RNA and the NEBNext Ultra™ II RNA Library Prep Kit (NEB, E7775) with rRNA depletion following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... The PCR products were cloned into the BsmBI-digested optimized sgRNA lentiviral expression vector LRG2.1T using a Gibson assembly kit (New England Biolabs; Cat. No. E2611). The LRG2.1T vector was derived from a lentiviral U6-sgRNA-EFS-GFP expression vector (Addgene ...
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bioRxiv - Cell Biology 2021Quote: ... mutant (GFP-FIP3-I738E-ABD) were engineered from the McCaffrey plasmids GFP-FIP3 and GFP-FIP3I738E using the Q5 Mutagenesis Kit (New England Biolabs, Ipswich, MA) following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: A total of 1 μg of RNA was used for cDNA library construction at Novogene using an NEBNext Ultra 2 RNA Library Prep Kit for Illumina (New England Biolabs; Cat.# E7775) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid encoding for Spike G614 variant was generated using the pTwist EF1alpha nCoV-2019 S 2xStrep plasmid and the Q5 site-directed mutagenesis kit (New England BioLabs, Evry, France) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Genomic (g)DNA was extracted from muscle biopsies (from n=27 participants) and isolated using the Monarch kit for DNA isolation (New England Biolabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Libraries were prepared with NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina according to the supplier recommendations (NEB). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... The sgRNA templates were generated by PCR (26) and used for synthesis of sgRNAs using NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S). The sgRNAs purified using MEGAclear Kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... at −80 °C prior to RNA extraction and library preparation (performed by Novogene Company Limited with NEB Next® Ultra™ RNA Library Prep Kit (NEB)) prior to RNA sequencing (paired-end 150 bp ...
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bioRxiv - Cancer Biology 2022Quote: ... The digested constructs were then purified with Ampure XP Beads and then ligated with the Quick Ligation Kit (NEB, Cat. No. M2200S).
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bioRxiv - Genomics 2022Quote: ... NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® according to the manufacturer’s instructions (New England Biolabs). Paired-end 100 bp reads were generated.
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England BioLabs; https://www.neb.ca). The 5 ‘ untranslated region (UTR ...
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bioRxiv - Microbiology 2022Quote: Complementary DNA (cDNA) libraries were constructed with the Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). cDNA concentrations were measured using a Qubit fluorometer with a dsDNA BR/HS kit (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was transcribed to full-length cDNA using the SMART-Seq253 protocol and RNA-sequencing libraries were generated using the NEBNext® Ultra™ II FS DNA library preparation kit (New England Biolabs) according the manufacturer’s protocol with DNA input below 100 ng ...
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bioRxiv - Neuroscience 2022Quote: The 16S rRNA V4 region was amplified using the following primers 515f-Y: GTGYCAGCMGCCGCGGTA and 806r-N: GGACTACNVGGGTWTCTAAT and The Q5® high-fidelity DNA polymerase kit (New England BioLabs, UK). 2μl of extracted DNA was added to a PCR reaction mix prepared by mixing a final concentration of 1X Q5 reaction buffer ...
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bioRxiv - Immunology 2022Quote: B cells were cultured for 48 hours before total RNA was extracted using Quick-RNA™ Microprep Kit (ZymoResearch) and mRNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation (New England BioLabs). Stranded Illumina libraries were prepared with Swift Rapid RNA Library Kit according to the manufacturer’s instructions (Swift Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... The pACYC184::attI1 recipient plasmid was created by assembling the attI1 sequence (from R388) into the pACYC184 plasmid backbone using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, United States). The PCR products required for the assembly were generated using the attI1_fw/attI1_rev and pACYC184_backbone_F/pACYC184_backbone_R primer pairs ...
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bioRxiv - Neuroscience 2022Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... 150 ng of RNA per sample was employed in the generation of libraries using the NEB Next Ultra Pure RNA library kit for Illumina (NEB, cat # E7770S) per manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... and a PCR-free library was obtained with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs). Approximately 200 million of 150 bases pair-end reads were generated using the HiSeq X System (Illumina) ...
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bioRxiv - Genetics 2022Quote: ... the truncated versions of cwr-1 and the histidine mutant plasmids were prepared using the Q5 Site-Directed Mutagenesis Kit (E0552S NEB, Ipswich, MA). Gibson assembly (E2611S NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... The library for RNA-Seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA). During the second cDNA synthesis dUTP was incorporated to maintain strand specificity ...
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bioRxiv - Cell Biology 2022Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA libraries were constructed using the NEBNext® Ultra(tm) II Directional RNA Library Prep Kit (New England Biolabs, Inc., Massachusetts, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: One microgram of total RNA from each sample was subjected to cDNA library construction using a NEBNext® Ultra non-directional RNA Library Prep Kit for Illumina® (New England Biolabs). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by column purification and on-column DNase digestion according to the TRIzol two-step protocol from the Monarch total RNA miniprep kit (NEB, cat. T2010S) or PureLink RNA mini kit (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries of input fractions from Figure S8 were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for illumina (NEB).
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were prepared from a starting amount of 100ng total RNA using the NEBNext® Ultra™ DNA Library Prep Kit for Illumina (E7370L, New England Biolabs) using ribosomal depletion as a selection method ...
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bioRxiv - Microbiology 2020Quote: ... 1-3 μg RNA was used to generate sequencing libraries with NEBNext Ultra™ RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) with poly(A ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg total RNA with RIN value above 7 was used for library preparation by Genewiz using NEBNext® UltraTM RNA Library Prep Kit for Illumina® and NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Size selection of Adaptor-ligated DNA was then performed using AxyPrep Mag PCR Clean-up (Axygen) ...
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bioRxiv - Plant Biology 2021Quote: ... Library concentration was further quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England BioLabs®, Inc.). Libraries were sequenced on one lane of the Illumina® HiSeq4000 platform to generate 150-bp paired-end reads.
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bioRxiv - Molecular Biology 2020Quote: ... sgRNA target site mutation in mouse Thap1 cDNA was generated by Q5 Site-Directed Mutagenesis Kit (NEB, see Table S3 for primers). WT or Thap1-/-Brca1Δ11 MEFs (1 × 106 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries for sequencing were prepared using NEBnext Ultra II directional RNA library prep kit for Illumina (New England Biolabs, Cat. No E7760L). Libraries were sequenced on an Illumina HiSeq 1500 instrument at the Laboratory of Functional Genomic Analysis (LAFUGA ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext Ultra II FS DNA library kit for Illumina with 384-unique indexes (New England Biolabs, Massachusetts, USA; Cat. No: E6617L). Libraries were sequenced using the HiSeq X10 with 150 bp paired-end chemistry (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... while libraries for Illumina sequencing were prepared by using a NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The synthesized oligo pool of CRISPRa library was PCR amplified with primers (table S5) including the BsmBI recognition sites using Phusion®High-Fidelity PCR Kit (NEB, E0553L). After purification with DNA Clean & Concentrator-25 (Zymo Research Corporation ...
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bioRxiv - Microbiology 2021Quote: ... resulting in ∼1 ml of turbid bacterial culture which was subsequently used as input for plasmid purification by miniprep kit (New England Biolabs, cat#T1010S). The pooled plasmid library DNA for each library was eluted in 35 μl of elution buffer and quantified ...
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bioRxiv - Microbiology 2021Quote: ... and ∼ 90 ng was used for one-step RT-qPCR using Luna® Universal one-step RT-qPCR kit (New England BioLabs Inc.) [10 min ...
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bioRxiv - Immunology 2021Quote: ... The linearized vector and the synthesized fragment in a 1:2 molar ratio were assembled with the NEBuilder HiFi DNA Assembly Kit (NEB, Ipswich, USA) at 50°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: A total amount of 0.4 ug of RNA was used for cDNA library construction at Novogene using an NEBNext® Ultra 2 RNA Library Prep Kit for Illumina® (cat NEB #E7775 ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNAs were reverse transcribed to cDNA using ProtoScript II reverse transcriptase with oligo dT and random primers (First Strand cDNA Synthesis kit New England Biolabs, Ipswich, MA). RT-PCRs specific for the Cas9 or actin gene of B ...
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bioRxiv - Microbiology 2020Quote: ... and one fragment of about 320 bp of the 3’-terminal region by 3’ RACE were amplified using Phusion High-Fidelity PCR Kit (New England Biolabs, MA, USA) under the following conditions [98°C ...
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bioRxiv - Plant Biology 2021Quote: ... mRNA was enriched with oligo(dT)-beads and cDNA was synthesized with random hexamer primers with the NEB Next Ultra RNA Library Prep Kit (NEB, Ipswich, USA). All libraries were sequenced using the Illumina NovaSeq6000 platform in paired-end mode with a read length of 150 bp.
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bioRxiv - Microbiology 2020Quote: ... The products of this reaction were prepared for Illumina sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed in a two-step manner using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, Cat#E6300S) and iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting single stranded cDNA was further In-vitro transcribed (IVT) using HiScribe T7 Quick High Yield RNA Synthesis Kit according to the manufacturer’s protocol (New England BioLabs Inc, NEB #E2050). The amplified RNA was than purified using Monarch RNA Cleanup Kit (New England BioLabs Inc. ...