Labshake search
Citations for New England Biolabs :
9001 - 9050 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... samples were harvested and RNA was isolated and reverse trancripted in cDNA following protocol manufacturer (LunaScript™RTSuperMix Kit, New England Biolabs Inc). The 20 μL reaction consisted in 4 μL of RT Mix completed with 16 μL of water with 100 ng of RNA ...
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bioRxiv - Microbiology 2021Quote: ... Samples were treated with chloroform and the aqueous phase was used in the Monarch® RNA Cleanup Kit (New England Biolabs Inc).
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bioRxiv - Cell Biology 2020Quote: ... of full-length SEC16A were generated by mutating 10 nucleotides in the silencer-select Sec16A siRNA (5’-CGGAGCUUCUGUUACGAGATT-3’, siRNA id: s19236, Thermo-Fischer Scientific) binding site using Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... pBGC was constructed by joining the amplified pBGT backbone and the gfp terminator region using the Gibson Assembly Cloning Kit (New England BioLabs, MA, USA). Resulting reaction was transformed by heat shock into NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2020Quote: ... we prepared a fragmented DNA library using a NEBNext Ultra II FS DNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Sequencing of a paired-end 2 × 150 bp mode on a HiSeq X system (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... were prepared using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina modules E7810L and E7595L (New England BioLabs) following the manufacturer protocol for use with inputs ≥ 100 ng with modifications to eliminate PCR-amplification steps.
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bioRxiv - Immunology 2021Quote: ... The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: The DNA library was prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions and sequenced using the Illumina MiSeq platform with a read length of 2 × 150 bp (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... <200nt isolates were immediately frozen at -80⍰°C while >200nt isolates were used as template for cDNA synthesis (LunaScript RT Mastermix Kit, New England Biolabs, MA, USA) and subsequent doublesex sexing PCR as previously described (25) ...
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bioRxiv - Microbiology 2022Quote: ... The rRNA-depleted total RNA was subjected to library preparation using NEBNext® Ultra™ II RNA Library Prep Kit (E7700, NEB) and barcoded with NEBNext Multiplex Oligos for Illumina (E7730 ...
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bioRxiv - Microbiology 2022Quote: ... All capped viral RNA was in vitro transcribed from the corresponding linearized plasmid using the HiScribe T7 antireverse cap analog (ARCA) mRNA kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... total RNA libraries were built using the NEBNext Ultra II library prep kit for Illumina (New England BioLabs inc., product code: E7775) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... the plasmid library was linearized by BsmbI digestion and ligated with TmU fragments with Quick ligation™ kit (New England BioLabs, M2200S) for 5 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: Poly-A libraries (figure 1 and related data) were constructed with the NEBNext Ultra RNA library prep kit for Illumina (New England Biolabs, Ipswich, USA), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... RNA integrity was validated by gel electrophoresis and 800 ng were reverse transcribed using the LunaScript® RT SuperMix Kit according to manufacturer’s instructions (E3010, New England Biolabs, Ipswich, MA). Real-time PCR was performed using the Blue ŚGreen qPCR Kit (F416 ...
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bioRxiv - Genetics 2022Quote: ... The tRNAs were re-phosphorylated and converted to small RNA sequencing libraries using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® kit (New England Biolabs). Size selection of 140-155 bp PCR amplified fragments (corresponding to 19-35 nt tRNA fragments size range ...
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bioRxiv - Physiology 2022Quote: ... 3-10 ng of immunoprecipitated DNA was used to generate adapter-ligated DNA libraries using the NEBNext® Ultra DNA library kit for Illumina (New England Biolabs, E7370L) and indexed multiplex primers for Illumina sequencing (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... 100bp strand-specific whole transcriptome libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Herfordshire, UK), following rRNA depletion with NEBNext rRNA Depletion Kit or Ribo-Zero Gold ...
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bioRxiv - Cell Biology 2022Quote: ... mutated FN constructs were PCR amplified and ligated into BamH1+Xho1 cut NSPI vector using NEBuilder® HiFi DNA Assembly kit (BioLabs, #E5520).
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bioRxiv - Genomics 2022Quote: ... wild-type and mutant NEAT1 RNA fragments were transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S) and labelled with Biotin using Biotin RNA Labelling Mix (Roche ...
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bioRxiv - Molecular Biology 2020Quote: Thermocycler was used to denature and anneal 5 µl of PCR product as recommended by manufacturer (EnGen™ Mutation Detection Kit, NEB, E3321S). Thermocycler was adjusted for heteroduplex formation as following ...
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bioRxiv - Microbiology 2020Quote: ... as previously described[7] Phage DNA libraries were prepared using NEBNext Ultra II FS library Prep and Kit for Illumina (New England Biolabs, Ipswich, USA). The sequencing was performed on the Illumina iSeq platform as part of a flowcell (2 x 151 ...
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bioRxiv - Synthetic Biology 2020Quote: ... were PCR amplified from genomic DNA of yeast strain BY474129 and cloned together with the bidirectional inducible Gal1-10 promoter into the PCR amplified pRSII42B backbone with OZL14 and OZL15 via Gibson assembly30 using NEBuilder Kit (NEB, catalog E2621). MMR-DN mutant genes were subsequently generated via site-directed mutagenesis using either Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... paired-end libraries with insert size of ~300 bp were also constructed using NEB Next® Ultra DNA Library Prep Kit (NEB, USA), with the standard protocol provided by Illumina (San Diego ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and cDNA libraries were made from each sample using the NEBNext RNA Ultra II Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). Barcoded samples were sequenced using the Illumina NextSeq 500 (Illumina ...
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bioRxiv - Genomics 2020Quote: ... and 10-12 day adult TRAP libraries were prepared with the NEBNext Ultra RNA library Prep Kit for Illumina (NEB, product E7530S). ChIP-seq libraries were prepared using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... Library quality was assessed by Bioanalyzer and the concentration was determined using the NEBNextv Library Quant Kit for Illumina® (New England BioLabs, E7630). Libraries were pooled and sequenced on the HiSeq4000 machine (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid encoding GFP-labeled dominant negative mutant version of human Rab5B isoform was introduced to plasmid #1008 using Q5 Site-Directed Mutagenisis kit (NEB, Cat# E0554S) according to manufacturer’s instructions using forward primer #1554 (AGTGGGAAAGaacAGCCTGGTATTAC ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA libraries for Illumina sequencing were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) following the manufacturer’s protocol in 0.5 of the recommended volume (due to low RNA quantity in such samples as shoot apical meristem) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Illumina paired-end libraries were constructed with the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... plus 280 nM for each primer and the standard reagents in the Phusion High-Fidelity PCR Kit (New England BioLabs, Ipswich, MA). Samples were then cycled under the following qPCR conditions ...
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bioRxiv - Microbiology 2020Quote: ... Five hundred nanograms total RNA was then reverse-transcribed in 20 μl reaction volume using the LunaScript RT SuperMix Kit (E3010, New England Biolabs, MA, USA). Quantitative PCR was performed by transferring 2 μl of the RT mix to the qPCR mix prepared with Luna Universal qPCR Master Mix (M3003 ...
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bioRxiv - Microbiology 2021Quote: ... and library construction were done using NEBNext® µLtra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswitch, MA, USA) following the manufacturer’s recommendation and starting with 3 μg of RNA per sample ...
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bioRxiv - Microbiology 2021Quote: ... HA tag sequences were added to the 3’ end of the hACE2 transgenic cDNA using a Q5® Site-Directed Mutagenesis Kit (NEB#E0554S) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Mutations were introduced into the ATP11A ORF using a The Q5® Sit-direct mutagenesis kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... Up to 10 ng of cDNA was used for the Illumina sequencing library construction using NEBNext® Ultra™ DNA Library Prep Kit (NEB). Paired ends sequencing was performed on NextSeq 500 (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Genomics 2020Quote: ... A cDNA library with insert sizes of 350-400 bp was prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, MA, USA) and paired-end sequenced on the HiSeq X ten platform (Illumina Inc. ...
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bioRxiv - Genomics 2021Quote: ... One microgram of mRNA was used as a template for first-strand cDNA synthesis using NEBNext® Poly(A) kit (NEB #E7490, New England Biolabs, Inc.) and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760 ...
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bioRxiv - Microbiology 2021Quote: ... This PCR product was cloned into pBHM2403digested with NotI and SacII by Gibson assembly using the NEB Hifi DNA Assembly Kit (New England Biolabs, Ipswich, MA). To generate a sgRNA expression cassette ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and ligated with Illumina sequencing adapters using a NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs, Ipswich MA, USA), and further PCR enriched with primers of P5 and P7 oligos ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were prepared using a NEBNext Ultra RNA Library Prep Kit and NEB Next Multiplex Oligos (New England Biolabs E7530L and E7335L). Library quality and size distribution was confirmed by running an Agilent High Sensitivity DNA assay (Agilent Genomics 5067-4626) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA library was constructed using the NEBNext® UltraTM RNA library preparation kit as per the manufacturers’ protocol (New England BioLabs, USA). mRNA was first purified via the addition of poly-T-oligo-attached magnetic beads ...
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bioRxiv - Genomics 2021Quote: ... Strand-specific RNA-seq libraries were constructed using the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs). The libraries with adapters were sequenced on the Novaseq 6000 (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... The sample preparation was performed according to the protocol “NEBNext Ultra II Directional RNA Library Prep Kit for Illumina” (NEB #E7760S/L). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... Transcript knockdown was confirmed in T0 and T1 plants using qPCR (qScript cDNA synthesis kit, QuantaBio®, and Luna qPCR mix; NEB®) according to manufactuers’ protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... sgRNA synthesis was performed by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA). Cas9 mRNA was prepared by in vitro transcription with the mMESSAGE mMACHINE T3 Transcription Kit (Life Technologies ...
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bioRxiv - Biophysics 2021Quote: ... The vimentin constructs (Y117L mutants and addition of a Spot tag) were produced using a Q5 Site-Directed Mutagenesis kit (Biolabs cat # E0554S) using the primer lists in Table 1 ...