Labshake search
Citations for New England Biolabs :
8001 - 8050 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Purified dsDNA templates were used as templates for T7 transcription reactions using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) and bio-16-CTP (Trilink ...
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bioRxiv - Genomics 2020Quote: A total of 1.5 μg of gDNA was end-repaired (NEBnext ultra II end repair kit, New England Biolabs, MA, USA) and purified using 1x AmPure beads (Beckmann Coulter ...
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bioRxiv - Genomics 2020Quote: Plasmids containing H2afz-FKBP.knock-in.BFP and Nelfb-Halo DNA repair templates were synthesized with long homology arms (∼800 bp) by Genewiz FragmentGene and assembled using the NEBuilder HiFi DNA Assembly Cloning kit (NEB E5520S). The Dendra2-RBP1 plasmid and guide were used as previously described10.
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bioRxiv - Genomics 2020Quote: ... An Illumina-compatible library was then prepared from 400 ng input gDNA with an NEBNext Ultra™ II FS DNA Library Prep Kit for Illumina (New England Biolabs) with a total of four PCR cycles to introduce dual indexed barcodes ...
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... Transposed DNA samples were purified using the Qiagen MinElute Kit (#28204) followed by amplification using 1x PCR master mix (NEB #M0541S) and 25 μM Ad1_noMX and Ad2.* indexing primer for 10-14 cycles ...
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bioRxiv - Molecular Biology 2021Quote: TCF20(1-1268)-mEGFP construct was generated from the TCF20-mEGFP plasmid using the Q5 Side-Directed Mutagenesis Kit (NEB, E0554S) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the libraries have been generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA). The libraries have been purified using the AMPure XP system ...
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bioRxiv - Molecular Biology 2021Quote: ... were used as template of the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, #E2050S, New England Biolabs), performed at 37°C for 16 hr ...
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bioRxiv - Developmental Biology 2020Quote: Libraries for DNA sequencing were prepared according to the instructions accompanying the NEBNext DNA Ultra II kit (catalog # E7645S; New England Biolabs, Inc). Libraries were sequenced on the NextSeq 500 following the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... SpCas9 was amplified by PCR and the PCR product was assembled with a −8.7 kb backbone construct using NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, USA) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Qualified RNA per sample was used to generate sequencing libraries by NEBNext Ultr RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... The gRNAs targeting SERPINB5 and Tnf were created by site-directed mutagenesis of pLenti-Il33gRNA6-puro using primers listed in Supplementary Table S2 and the Q5® Site-Directed Mutagenesis Kit (NEB) according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Two gRNAs targeting the second and the last exon of spel1 were cloned with the Gibson Assembly® Cloning Kit (New England Biolabs) into a BbsI (10,000 units/ml ...
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bioRxiv - Biochemistry 2020Quote: Mutated variants of AtXOAT1 were generated by site-directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) according to the manufacturer’s instructions using pGEN2-DEST-AtXOAT1 as a template ...
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bioRxiv - Genomics 2020Quote: ... ChIP-seq libraries were prepared for sequencing using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, #E7103). The detailed ChIP-seq protocol can be found in Supplementary materials.
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bioRxiv - Genomics 2020Quote: ... Libraries for ChIP-seq were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, NEB #E7370) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: RNAseq libraries were prepared with the “protocol for library preparation of Intact RNA using NEBNext rRNA depletion kit (Bacteria) (NEB#E7850, NEB#78860) and NEBNext Ultra II directional RNA library prep kit for Illumina (NEB#E7760 ...
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bioRxiv - Genetics 2021Quote: ... PCR products were assembled and cloned into the pW8-dhdWT vector (Tirmarche et al., 2016) previously digested by KpnI and BamHI using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, #E5520S). dhdΔDRE transgene was integrated in the PBac{attP-3B}VK00031 platform (62E1 ...
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bioRxiv - Genetics 2020Quote: ChIP-seq libraries were built using the NEB Next UltraII DNA library Prep kit for Illumina (New England Biolabs #E7645S/L) and Agencourt Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... The transcriptome library was prepared using NEB ultraII RNA library preparation kit as per the manufacturer’s recommended protocol (NEB, MA, USA) and the libraries then underwent size selection ...
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bioRxiv - Genomics 2021Quote: ... 1μg of total RNA was used to enrich mRNA using NEB Magnetic mRNA isolation kit according to the manufacturer’s instructions (NEB, MA, USA). cDNA was synthesized and ligated to sequence adapters ...
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bioRxiv - Genetics 2020Quote: ... pREP81-FLAG vector and plasmids carrying wild type and mutated forms of rrp1+ and rrp2+ as well as domains of rrp1+ gene used in the yeast two hybrid system were constructed using the Gibson Assembly® Cloning Kit (NEB). All primers used to amplify gene sequences by PCR are listed in Table S3 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and used this as template to construct DNA libraries using a NEBNext Fast DNA Library Prep Set for Ion Torrent kit (New England Biolabs, Canada). Each sample library contained a unique barcode adapter (NEXTflex DNA Barcodes for Ion Torrent ...
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bioRxiv - Microbiology 2020Quote: ... Shotgun metagenomic sequencing libraries were prepared directly from one aliquot of each sheared genomic DNA sample using the NEBNext Illumina library preparation kit according to the manufacturer’s directions (New England Biolabs, MA, USA). Samples were then sequenced with 2×250 bp cycles of v2 Miseq chemistry (Illumina ...
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bioRxiv - Genomics 2021Quote: This material was used for generating sequencing libraries using the Small RNA library prep Set for Illumina (Multiplex compatible) kit (NEB E7330). Typically ...
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bioRxiv - Genomics 2021Quote: ... The resulting rRNA-depleted RNA was used for library construction with the NEB ultra II directional RNA-seq kit (NEB E7760). The library was sequenced on illumina Miseq (75 bp paired-end sequencing) ...
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bioRxiv - Genomics 2021Quote: ... and a portion corresponding to 1 µg input RNA was converted to cDNA using the LunaScript RT SuperMix cDNA synthesis kit (NEB E3010). 1% of the cDNA was used for each qPCR reaction performed with the Luna Universal qPCR Mastermix (NEB M3003 ...
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bioRxiv - Genomics 2021Quote: ... The elution product was directly used for second-strand synthesis using reagents from the NEBNext Ultra II Directional RNA Library Prep Kit (NEB E7760). 8 μL of Second Strand Synthesis Reaction Buffer ...
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bioRxiv - Genomics 2020Quote: ... Following the manufacturer’s instruction 3μg RNA per group was used for sequencing libraries were generated using a NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). The index-coded samples were clustered on a cBot Cluster Generation System using the TruSeq PE Cluster Kit v3-cBot-HS (Illumina ...
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bioRxiv - Genomics 2020Quote: ... Libraries for ChIP-seq were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, NEB #E7370) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the NEB Library Prep protocol of the manufacturer was followed and samples were adaptor ligated using the NEBNext End Prep kit (NEB, #E7645). The adaptor-ligated DNA fragments were indexed with the NEBNext Multiplex Oligos for Illumina kit (NEB ...
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bioRxiv - Immunology 2021Quote: Point or deletion mutations were prepared from human or mouse CRT expression clones in pCMV3-C-Myc (SinoBiological) using the Q5® Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s protocol and primers designed using the NEBaseChanger™ web tool ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA libraries were prepared at Génome Quebec (Montreal, QC, Canada) with the Ultra II NEB DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). Library quality and size distribution was determined on a high-sensitivity DNA chip on the Agilent 2100 bioanalyzer system ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA libraries were prepared on a template of randomly fragmented mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA). Since 8 samples out of 20 initially contained < 200 ng of total RNA ...
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bioRxiv - Microbiology 2021Quote: ... The resultant PCR fragments were inserted into the BamHI site in pAK405 by NEBuilder HiFi DNA assembly cloning kit (New England Biolabs, Inc.). These plasmids were independently introduced into SYK-6 and its mutant cells by triparental mating ...
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bioRxiv - Biochemistry 2020Quote: ... molecules used in this study were synthesized by in vitro transcription using HiScribe™ T7 Quick High Yield RNA Synthesis Kits (New England Biolabs), or transcribed directly in the in vitro translation (IVT ...
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bioRxiv - Synthetic Biology 2021Quote: Illumina sequencing libraries were generated from 100-200 ng BAC DNA using NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs E7805S) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The BAG1 promoter fragment and EGFP ORF or mCherry (ORF) were cloned by NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S) into the vector backbone that was produced by double enzymatic digestion of pTUB1:YFP-mAID-3HA ...
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bioRxiv - Microbiology 2021Quote: ... Point mutations to the binK sequence on the plasmid were designed using the NEBaseChanger tool and constructed with the Q5 site-directed mutagenesis kit (New England BioLabs, Inc.). The constructed plasmid was transformed into either electrocompetent or chemically competent DH5α λpir E ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared using the rRNA-depleted RNA as input for NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA). Libraries were pooled and submitted for 2 × 150 bp paired-end sequencing on an Illumina NextSeq High-Output platform at the Center for Genome Sciences and Systems Biology at Washington University in St ...
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bioRxiv - Developmental Biology 2021Quote: ... using 200 ng of sheared gDNA and 10 PCR cycles using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S). The DNA libraries were indexed with unique dual barcodes (8bp long) ...
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bioRxiv - Cell Biology 2021Quote: Samples were isolated in RNA extraction buffer and RNA isolated using the Monarch® Total RNA extraction Miniprep kit (T2010S; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Spike-in RNA of the bacteriophage MS2 served as an interal control and was detected with Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) using corresponding primers and probe (MS2_F ...
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bioRxiv - Bioengineering 2020Quote: ... The pool of partially double stranded DNA templates was used for in vitro transcription with HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England BioLabs) and the RNA was purified with TRizol (Ambion) ...
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bioRxiv - Bioengineering 2021Quote: ... palustris cultures at mid-exponential phase in duplicate using the Monarch® Genomic DNA Purification Kit (New England Biolabs® Inc.). Amplified PCR product using primers in Supplementary Table VI was diluted to 109 copies of amplicon/μL per the following equation.
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bioRxiv - Biochemistry 2020Quote: ... the sGFP sequence was deleted and a 6xHis-tag placed at the C-terminus of AcrB followed by a stop codon using the Q5® site-directed mutagenesis kit (New England Biolabs) - AcrB contains two Histidine residues at its C-terminus ...
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bioRxiv - Biochemistry 2020Quote: ... The G288D mutation was then generated from this pET15b-AcrB-6xHis plasmid using the Q5® site-directed mutagenesis kit (New England Biolabs). All constructs were verified by DNA sequencing (Eurofins MWG).
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bioRxiv - Biochemistry 2020Quote: ... The G288D mutation was generated from this pBR-AcrBWT plasmid using the Q5® site-directed mutagenesis kit (New England Biolabs). All constructs were verified by primer walking DNA sequencing (Eurofins MWG).
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bioRxiv - Biochemistry 2021Quote: Illumina sequencing libraries were prepared from 1ng to 10ng of purified CUT&RUN DNA using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs # E7645) as previously (Liu et al ...
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bioRxiv - Biochemistry 2020Quote: ... PCR products were cloned into pET22b-mCherry vector using the NdeI and SpeI restriction sites and joined using Gibson Assembly® Cloning Kit (New England Biolabs) according to manufacturer’s instructions.