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Citations for New England Biolabs :
8201 - 8250 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: GQES7-a and GQES7-b were synthesized in vitro by transcription (HiScribe™ T7 High Yield RNA Synthesis Kit; New England Biolabs). GQes3 and mutes3 ...
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bioRxiv - Genomics 2020Quote: ... 48hr APF TRAP libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, product E7760S) and 10-12 day adult TRAP libraries were prepared with the NEBNext Ultra RNA library Prep Kit for Illumina (NEB ...
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bioRxiv - Biophysics 2020Quote: ... and the corresponding RNAs were synthesized via in vitro transcription using the HiScribeTM T7 Quick High Yield RNA Synthesis Kit (New England Biolabs Inc.) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... reverse primer ACA AGG GCT TTC TCT CAT AAT GAG ATG CTC and the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Cells were transfected with 50 nM siRNA using Dharmafect 1 for 48 h ...
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bioRxiv - Genetics 2020Quote: ... The purified DNA (200 ng) was subject to library preparation using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB). Libraries with barcodes were pooled and sequenced on the Illumina HiSeq 1500 platform ...
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bioRxiv - Genetics 2020Quote: Libraries were prepared from 2 ng of DNA using the NEBNext Ultra II FS DNA library prep kit (New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and mutated into a constitutively active form (pEGFP-C1-ERαY537S)29 using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E054S).
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bioRxiv - Genomics 2021Quote: ... and then ligated into pcDNA 3.1 3xHA cut with HindIII and NotI using the Quick Ligation kit (New England Biolabs cat # M2200L) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... These promoters and first ten amino acids of YmoA and H-NS were fused in frame to lacZ and cloned into a BamHI- and SalI-digested pFU99a using the NEBuilder HiFi DNA Assembly kit (New England Biolabs, Inc) electroporated into Y ...
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bioRxiv - Molecular Biology 2020Quote: ... The domain deletion mutants of pCS2+ FLAG-tagged SRSF1 construct were generated using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The primers sequences are given in Supplementary Table S1.
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bioRxiv - Bioengineering 2020Quote: ... 200-500 ng of the purified PCR product was used as DNA template in 25 μl of coupled in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were created using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and sequencing performed on an Illumina NextSeq 500 using a NextSeq High Output Kit ...
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bioRxiv - Genomics 2020Quote: ... 700 nanograms of extracted DNA was utilized as starting material in NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs, USA). The DNA was fragmented using focused ultrasonicator (Covaris M220 ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared with random primers using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760L) with NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Pathology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, MA, USA) following manufacturer’s recommendations and index codes were added ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries from the extracted RNA were generated using the NEBNext® RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Synthetic Biology 2021Quote: ... Standard PCR-based amplification to add overlap-adaptors and Gibson-style assembly methods (HiFi Assembly Kit, New England Biolabs, Ipswich, Massachusetts) were used to assemble the final vectors ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products (donor-5’biotin, donor-unmodified) were gel purified using the Monarch DNA Gel Extraction Kit (New England Biolabs, T1020S) and eluted in 20μl embryo transfer water (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Microbiology 2020Quote: ... and incubated at 37°C for 18 hours and the plasmid was extracted using the Monarch Plasmid Miniprep Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Mutations to the specificity of guides in pTREX-n-Cas9-noTags was performed using a Q5 mutagenesis kit (NEB, Ipswich, Massachusetts). Guides targeting the mitochondrial glutamate dehydrogenase (mGDH ...
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bioRxiv - Microbiology 2021Quote: ... then an RNAseq library was prepared with the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was then performed in single read mode on a HiSeq 3000 system ...
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bioRxiv - Microbiology 2021Quote: ... Pair-end reads libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) and sequenced on an Illumina Hiseq platform ...
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bioRxiv - Microbiology 2021Quote: ... and 500ng DNA was used as input for the NEBNext Ultra II FS DNA Library Prep kit (New England Biolabs, USA), following the manufacturer’s instructions with modifications as follows ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA from each library was purified from an entire 1ml cell pellet using the Monarch Genomic DNA Purification Kit (New England Biolabs, USA). Samples were eluted in 50µl nuclease-free water ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sequencing libraries were generated from the qualified DNA samples using the NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... We then performed in vitro transcription from these amplicons using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs), and purified the resulting RNA using a GeneJET RNA Purification Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... The digested samples were loaded on an agarose gel to detect and purify fragments ranging from 100-500 bps using the Monarch® DNA Gel Extraction Kit (New England Biolabs). The fragmented gDNA was incubated for 1 hr with 10 μM PhuS in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting amplicon was assembled with a hHBB-Nluc sequence that lacked a 3’ UTR but maintained a unique barcode using a NEBuilder HiFi Assembly Kit (NEB, ES2621).
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared following the protocol from NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7770S) and indexes (NEB #E7335S and #E7500S).
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bioRxiv - Immunology 2020Quote: ... The PCR products were purified from a 1% agarose gel using the Monarch DNA Gel Extraction kit (New England Biolabs, USA) and send for Sanger sequencing provided by Edinburgh Genomics ...
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bioRxiv - Immunology 2020Quote: ... The mAbs were reverted to the UCA sequence in a step-by-step manner using the Q5® Site-Directed Mutagenesis Kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were cloned and performed Gibson assembly together with the triple-digested vector pCVD443 (NEBuilder HiFi DNA Assembly Cloning Kit E5520S, New England BioLabs Inc.). Assembly mixture was then used to transformed E ...
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bioRxiv - Biochemistry 2020Quote: Mutagenesis of the 5-HT2C construct was performed according to the Q5® site-Directed Mutagenesis Kit protocol (New England BioLabs). In brief ...
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bioRxiv - Genomics 2021Quote: ... cDNA synthesis by RT was performed immediately after DNaseI digestion of placental RNA with the ProtoScript®II First Strand cDNA Synthesis Kit (E6560, New England Biolabs) and random hexamer primers ...
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bioRxiv - Genomics 2021Quote: ... 250–500 ng extracted RNA was used to prepare libraries with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs #E7760S) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... RNA-seq libraries were constructed using 1ug DNA-free total RNA with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA. NEB#E7770). Poly (A ...
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bioRxiv - Genomics 2021Quote: ... 1μg of total RNA per sample was processed using the NEBNext Ultra II Directional RNA library prep kit from NEB (E7760L) utilising the NEBNext Poly(A ...
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bioRxiv - Genomics 2021Quote: ... were used as template of the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit; #E2050S, New England Biolabs), performed at 37°C for 16h ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA libraries were prepared from total RNA samples with an RIN ≥ 8.0 using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (New England BioLabs). An Illumina NovaSeq 6000 machine with an S4 flow cell was used to generate pairwise 100 bp reads (performed by the Yale Center for Genome Analysis).
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bioRxiv - Genetics 2020Quote: ... All DNA fragments were assembled using the NEBuilder® HiFi DNA Assembly Cloning Kit (Catalog number: E5520S, New England Biolabs, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... An Illumina DNA library was prepared with the NEBNext® Ultra™ II FS DNA Library Prep Kit (NEB, Ipswich, USA) and multiplex barcoded with NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 2 ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...
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bioRxiv - Genetics 2020Quote: ... The PCR products were purifified using the FastPure Gel DNA Extraction Mini Kit (Vazyme, DC301-01, Nanjing, China) and then digested by BamHI and HindIII restriction enzymes (New England Biolabs, Inc.). C-terminal EGFP-tagged mouse full-length Pabpn1l was cloned into pcDNA3.1(+ ...
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bioRxiv - Cell Biology 2021Quote: ... containing TauRD-I260C for fluorescent labeling were constructed by mutagenesis of the pHUE-TauRD (P301L/V337M) plasmid using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Cell Biology 2021Quote: ... RNA-seq libraries were generated with 300 ng of total RNA using NEB Next Ultra II RNA Library prep kit (NEB, E7770) according to the vendor’s protocol ...
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bioRxiv - Genomics 2021Quote: We generated three replicates of +/- doxycycline-treated RNA-sequencing libraries with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7765S). We quantified and assessed the quality of the libraries with an Agilent 2200 Tapestation instrument ...
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bioRxiv - Microbiology 2021Quote: ... OtownIsak and SummerBoyErik were prepared using NEBNext® Ultra II FS library prep kit for Illumina (New England Biolabs, Ipswich, USA), while the sequencing library for Hovsa was prepared with Nextera® XT DNA Kit (Illumina ...
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bioRxiv - Immunology 2021Quote: ... The D614G Spike was generated by introducing the corresponding mutation in the Wuhan reference strain using Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Physiology 2020Quote: ... Mutagenesis of Ser125 to Arg in GPT and Ser153 to Arg in GPT2 17 was performed with the Q5 site-directed mutagenesis kit from NEB (#E0554). Adeno-associated viruses (AAV ...