Labshake search
Citations for New England Biolabs :
7951 - 8000 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... RNA-seq libraries were prepared in two biological replicates using NEBNext Ultra II Directional RNA Second Strand Synthesis Module and NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) as per manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... The coding sequence of each gene was amplified by RT-PCR on adult testis cDNA preparations and cloned into Y2H/Y3H vectors using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The specific alleles of each gene family member used in this study are provided in Supp ...
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bioRxiv - Genetics 2024Quote: ... Two micrograms of whole mosquito-derived or 200ng of hemolymph-derived total RNA were used for first-strand synthesis with the LunaScript RT SuperScript Kit (NEB). Gene expression analysis was performed with quantitative real-time PCR (qPCR ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA), and 2 x 150 bp paired-end sequencing (Q30 > 85% ...
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bioRxiv - Genetics 2024Quote: ... 500 ng of Total RNA was utilized as the input for sequencing library construction with the NEB Next small RNA library prep kit (New England Biolabs), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: The mRNAs for cell-free protein expression were transcribed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were generated using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Sequencing was performed in the NovaSeq 6000 System (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... an in-tube treatment with DNase I was performed using Monarch® DNase I and RNA was purified using the Monarch® RNA Cleanup Kit according to manufacturer’s instructions (New England Biolabs). Isolated RNA (0.5 - 1 μg ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg was reverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit with oligo-dT primers and murine RNase inhibitor (New England Biolabs). Quantitative PCR was performed in technical triplicate using 20 ng cDNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNA libraries were constructed using the NEBnext Ultra II Directional RNA Library Preparation Kit with polyA selection and dUTP method (New England BioLabs) and sequenced on an Illumina NovaSeq 2×150bp ...
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bioRxiv - Cell Biology 2024Quote: α-Synuclein-A140C was generated from the pT7-7 aSyn WT vector using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the libraries for RNA sequencing were generated from fragmented DNA with eight cycles of amplification using a NEB Next Ultra DNA Library Prep Kit (New England Biolabs). After the libraries were quantified using the Bioanalyzer (Agilent) ...
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bioRxiv - Microbiology 2020Quote: The real-time PCR technique was performed for NDV detection using Luna Universal One-Step RT-qPCR Kit E3005E (New England BioLabs, USA) following the manufacture procedures ...
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bioRxiv - Plant Biology 2020Quote: ... YFP and CESA6 genomic sequence through Gibson Assembly method using a Gibson Assembly Master Mix kit (New England Biolabs, Ipswich, MA). The construct was verified by DNA sequencing ...
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bioRxiv - Microbiology 2020Quote: ... was in-vitro transcribed from 1 μg of these templates using the HiScribe T7 High Yield RNA synthesis kit (NEB, E2040S). Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... the pT7-SVmCherry plasmid was first linearised with XhoI and used as a template for in vitro RNA transcription with HiScribe T7 ARCA mRNA kit (New England Biolabs, #E2065S). Transcribed genomic RNA was transfected into BHK-21 using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Expression of mRNA transcripts for PfRFC1 gene analysis were carried out using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Inc.), on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... libraries were prepared using the NEB Ultra II Library Preparation Kit and NEBNext® Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs). The generation of the libraries followed manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: E248R deletion mutant proteins of distinct domains (Δ constructs) were generated from E248R WT plasmid by site direct mutagenesis using the Q5 mutagenesis kit (New England Biolabs) as follows ...
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bioRxiv - Cell Biology 2020Quote: ... The presence of the PCR products was confirmed by gel electrophoresis and the products were then purified by Monarch® PCR & DNA Cleanup Kit (New England Biolabs).
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bioRxiv - Genetics 2021Quote: For CUT&RUN-sequencing libraries were made starting from 10 ng of CUT&RUN DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645S) using the following PCR program ...
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bioRxiv - Genetics 2021Quote: ... DNase libraries were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB, cat. # E7645), with double-sided SPRI size selection (Agencourt AMPure XP beads ...
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bioRxiv - Genetics 2021Quote: ... and a 50-μl aliquot containing between 832-841 ng was provide to the KU Genome Sequencing Core for library construction using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L), incorporating unique dual-indexing (NEB ...
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bioRxiv - Genetics 2021Quote: ... We converted oligo(dT)-selected RNA into a cDNA library for mRNA sequencing using the NEBNext Ultra™ Directional RNA Library Prep Kit for Illumina (NEB) in accordance with the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 ng of m6A-containing mRNAs or pre-immunoprecipitated mRNAs (the input) were used for library construction by the NEBNext ultra RNA library preparation kit (NEB, E7530). High-throughput sequencing was conducted on the illumina HiSeq X sequencer with a paired-end read length of 150 bp following the standard protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequencing libraries were made using the NEB Next Ultra II DNA library kit (New England BioLabs Inc, Ipswich, MA, Cat #E7645S) and were sequenced on the Illumina Hi-Seq4000 using 50bp single-end reads at the Northwestern Sequencing Core (NUCore).
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bioRxiv - Microbiology 2021Quote: ... A plasmid encoding an eGFP-containing EBOV antigenome was generated through assembly of fragments using the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs (NEB)) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolab E7420L) and NEBnext Multiplex Oligos for Illumina Dual Index Primers (New England Biolabs E7600S), using provided protocols and 500 ng of total RNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... corresponding to a partial ASY3 DEL allele was excised and purified with a Monarch® DNA Gel Extraction kit (New England Biolabs). Purified PCR products were cloned into pCR™4-TOPO® vector using a TOPO™ TA Cloning™ for Sequencing Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Libraries were prepared with 50 ng of DNA using the NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs®) as per the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA libraries for RNAseq analysis were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, NEB) and NEBNext rRNA Depletion Kit (Human/Mouse/Rat ...
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bioRxiv - Developmental Biology 2020Quote: ... A 250-300 bp insert cDNA library was generated by using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530S). Transcriptome sequencing was performed on an Illumina NovaSeq 6000 ...
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bioRxiv - Immunology 2021Quote: ... Illumina adaptors were ligated using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England BioLabs, USA) according to the manufacturer’s protocol and sequencing in the Illumina platform with the PE150 mode ...
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bioRxiv - Developmental Biology 2021Quote: ... Library preparation (from precipitated material and input chromatin as control) was performed using NEBNext Ultra II DNA library Prep Kit (New England Biolabs, #E7645S) without size-selection to maintain sample complexity and according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... The purified rRNA-depleted samples were converted to cDNA as per the NEBNext ultra II RNA library prep kit for Illumina (NEB, E7770L). Total RNA from the rest of the samples was converted to cDNA according to the ARTIC amplicon sequencing protocol for SARS-CoV-2.artic ARTIC protocol primer artic schemes for SARS-CoV-2 (Version 2 ...
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bioRxiv - Microbiology 2022Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep kit for Illumina with NEBNext® Poly(A) mRNA Magnetic Isolation Module (both New England BioLabs). Samples were adjusted to 400 ng total RNA and spiked with diluted 1/100 ERCC Spike-In Mix (4456740 ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA preparations were then used directly for qPCR (200 ng total RNA per reaction) using the Luna Universal One-Step RT-qPCR Kit (NEB, E3006) and target-specific primer/probe sets (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... The mixture was incubated for 3 days at 37 °C in the dark for conjugation and purified for 3 rounds using Monarch® PCR & DNA Cleanup Kit (5 μg) (Cat# T1030S, NEB) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... total RNA (1 µg) was transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (Cat. No. E6560; New England BioLabs). Quantitative real-time PCR was performed with 20 ng of cDNA in a total reaction volume of 10 μL using Luna® Universal qPCR Master Mix (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... was used to generate RNA-seq libraries using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (New England BioLabs, Inc) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampus RNA-seq libraries were prepared in accordance with New England Biolab protocols (NEBNext_ Ultra II RNA library Prep kit for Illumina, NEB, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis was performed from 1 µg total RNA using the ProtoScript® First Strand cDNA Synthesis Kit (New England Biolabs, NEB) with the provided random primer mix according to the suppliers’ instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng of two replicates each of AbmR co-purified RNA and rRNA-depleted induced E.coli control RNA were converted into RNA-seq libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total and nascent RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760S) following the rRNA depleted RNA protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The amplicon was purified with a Qiagen PCR purification kit and digested with the restriction enzymes KpnI and HindIII (NEB, UK), followed by ligation using T4 DNA ligase (Fermentas ...
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bioRxiv - Molecular Biology 2020Quote: ... and the next two fragments were inserted into resulting plasmid using NEBuilder®HiFi DNA Assembly Cloning Kit (New England Biolabs). Fragments were amplified with primers (Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... from COLO205 cells was subjected to one-step RT-qPCR analysis using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005S) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The sequencing library was prepared using the NEB Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645). Paired-end ...
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bioRxiv - Genomics 2020Quote: ... ~200 ng of gDNA was used for library preparation with the NEBNext DNA Ultra II Library Prep Kit (New England Biolabs # E7645S) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified with the oligonucleotide cleanup protocol as described in the Monarch PCR & DNA Cleanup Kit 5 µg user manual (NEB #T1030). Clean PCR products were sequenced using Sanger methods by Eton Biosciences (https://www.etonbio.com/ ...