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Citations for New England Biolabs :
8051 - 8100 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... to obtain the amplified PCR product which was then purified (Quiagen Gel purification kit) and digested with Nco1 and BamH1 restriction endonucleases (NEB, India) for inserting the gene into the pTrc99A expression vector ...
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bioRxiv - Biophysics 2021Quote: ... Mutagenic PCR to obtain the D614G amino acid change into both untagged and 161/345A4-tagged SΔTM constructs was done using the primers S2_D614_Q5-F and S2_D614_Q5-R (Table S1) and the Q5® Site-Directed Mutagenesis Kit (NEB®, Ipswich, MA, USA) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were prepared using with 1 μg of total RNA and mRNA isolation step was done by using NEBNext poly(A) mRNA Magnetic Isolation kit (New England Biolabs, E7490S). The cDNA libraries were generated by the “dUTP” method for strand-specificity using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... and the DNA-free samples consisting of RNA were converted to cDNA using M-MuLV Reverse Transcriptase kit (New England BioLabs, USA) according to the instructions provided by the supplier ...
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bioRxiv - Cell Biology 2021Quote: ... RNA sequencing libraries were prepared using the NEB Next Ultra RNA Library Prep Kit for Illumina using manufacturer’s instructions (NEB, Ipswich MA). The sequencing library was validated on the Agilent Tape Station (Agilent Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were chosen to minimize predicted off-target effects with maximum cleavage efficiency and were synthesized by in vitro transcription as previously described26 using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S) or ...
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bioRxiv - Cancer Biology 2020Quote: ... The Nup88 construct lacking the coiled coil domain (Nup88∆C) was created by inserting a stop codon after 584aa through site directed mutagenesis (Q5 Site Directed Mutagenesis Kit, NEB-E0554S) using pEGFPC1-Nup88 as template ...
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bioRxiv - Cancer Biology 2020Quote: ... was digested with BbsI and the gRNAs targeting E6 and E7 genes from HPV18 and HPV16 were designed using NEBuilder® HiFi DNA Assembly cloning kit (NEW ENGLAND BioLabs). Briefly ...
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bioRxiv - Cell Biology 2021Quote: The components of both cassettes were first PCR-amplified from other vectors and subsequently ligated together using the NEBuilder® HiFi DNA Assembly Cloning kit (NEB) to generate the resulting pENTR-eGFP-attP(bxb)-*BsdR and pENTR-mCherry-attP(C31)-*BleoR vectors ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The single-stranded labeled probes were finally cleaned up using the Monarch PCR & DNA cleanup kit (New England Biolabs®, T1030) following the oligonucleotide cleanup protocol ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was stored short-term at −20°C or long-term at −150°C or immediately used in a retrotranscription reaction (ProtoScript® AMV First Strand cDNA Synthesis Kit, New England Biolabs or SuperScript® III First-Strand Synthesis System ...
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bioRxiv - Neuroscience 2021Quote: ... Point variants were introduced into hFibcd1 cDNA while cloned in the intermediate pDONR201 plasmid using the Q5 site-directed mutagenesis kit (NEB #E0554), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA, USA inc.) for Illumina following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting fragment was then checked by 1% agarose gel and purified with the Monarch® DNA Gel Extraction Kit (New England Biolabs). The purified fragment was precipitated by ethanol precipitation and resuspended in buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... Quantification of libraries prior to sequencing used qPCR with primers specific to the Illumina P5 and P7 adaptor sequences and standards from the NEBNext Library Quant Kit (NEB, E7630S). Sequencing of prepared libraries was performed using an Illumina MiniSeq with the 75-cycle high-output kit ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR was performed with primers specific for target genes (see Supplementary Table 1 for the list of primers) using the Luna universal One-Step RT-qPCR kit (NEB; E3005). Experiment was performed using the QuantStudio3 (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... libraries were prepared from purified DNA using “NEBNext Ultra II DNA Library Prep Kit for Illumina” from New England BioLabs (NEB, #E7645), as per the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2020Quote: 20-33 kD subregions of NRPD1 and RDR2 polypeptides were expressed in vitro using a PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs). Briefly ...
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bioRxiv - Synthetic Biology 2021Quote: ... The plasmid of pAC-BETAipi-ispA/dxs was constructed by using Gibson assembly kit (Gibson Assembly® Master Mix, New England Biolabs). The insert was amplified from genomic DNA of E ...
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bioRxiv - Synthetic Biology 2021Quote: ... was linearized by PCR with oligos designed to flank the metY gene (Table S5) and the modified metY(AAC) gene was then inserted using homologous recombination (NEBuilder HiFi DNA Assembly kit (NEB#E2621)) ...
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bioRxiv - Microbiology 2020Quote: ... was subjected to first strand and second strand cDNA synthesis followed by adaptor ligation and enrichment with a low-cycle according to the instructions of NEBNext” UltraTM RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit”2.0 (Life Technologies ...
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bioRxiv - Systems Biology 2021Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Systems Biology 2020Quote: ... rRNA depletion and library preparation for sequencing was completed with the NEBNext protocol (‘Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Systems Biology 2020Quote: ... the samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Plant Biology 2021Quote: ... was used as input for library preparation using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs Inc). Libraries were sequenced using Illumina NextSeq High Output 75 bp pair-end (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: Synthesis of RNA in vitro was carried out as previously described using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050) [35 ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with an additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Genetics 2020Quote: ... Followed by a Dual index library prep with the DNA NEBNext® Ultra™ II Library Prep Kit (New England Biolabs), using a quarter of the recommended volumes with the Dual Index Primers Set ...
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bioRxiv - Genetics 2021Quote: ... Libraries for sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645), as described by the manufacturer but using half volumes of all reagents and starting material ...
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bioRxiv - Microbiology 2021Quote: ... Mutant of the dGTPase gene of Shewanella putrefaciens CN-32 was constructed using Q5 Site directed Mutagenesis kit (NEB, cat. #E0554S) using primers presented in Supplementary Table S5.
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bioRxiv - Microbiology 2020Quote: ... qRTPCR was performed with primers mentioned in Table S3 with Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs). Log2 normalized RNA abundances were calculated using ValidPrime signal as reference (51).
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bioRxiv - Microbiology 2021Quote: ... and fifteen repeats of the FG-repeats were amplified from genomic DNA using primers (Table S2) with Phusion High-Fidelity PCR Kit (NEB - E0553S). The PCR products were purified with GenElute PCR Clean-Up Kit (Sigma-Aldrich NA1020) ...
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bioRxiv - Microbiology 2021Quote: ... while the Zymo Mock sample was prepared with NEB Next Ultra II DNA library prep kit for Illumina (New England Biolabs, USA) following the manufacturer’s protocols and sequenced using the Illumina MiSeq platform.
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed either directly on the inactivated supernatants or on extracted RNA using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005E) in a QuantStudio 6 thermocycler (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: The gene encoding the C-terminal 6xHis GlcNAc-1-kinase NahK from Bifodobacterium longum JCM1217 was inserted into a pBAD/HisB backbone using a NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs) following manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Double-stranded single-indexed libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB #E7645S) and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 and 2 (NEB #E7335S ...
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bioRxiv - Microbiology 2021Quote: ... and all of the point mutants were constructed using Mut Express II Fast Mutagenesis Kit V2 (Vazyme, C214) and DpnI endonuclease (NEB, R0176S). Primers used for plasmids construction were listed in Supplementary table 1 and table 2 ...
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bioRxiv - Microbiology 2020Quote: ... A band at approximately 1 kb was cut out and DNA extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs, US) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the sgRNA cassette from lentiCRISPRv2 was removed by digestion with KpnI and EcoRI and the plasmid re-ligated using annealed repair oligo pair 1 (see Table S3) and Quick Ligation™ Kit (M2200L, NEB). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-miRNAs were purified on denaturant polyacrylamide gel and long pri-miR-K10/12 derived transcripts (up to ∼3 kb) were salt purified using Monarch® PCR and DNA cleanup kit (New England BioLabs). After acidic phenol extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2021Quote: ... EF1α Lamp1-mCherry plasmid was extracted from successful transformants with the Monarch® Plasmid Miniprep Kit (New England BioLabs Inc., USA), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... was used to generate RNA-seq library using NEBNext Ultra Directional RNA library Prep Kit for Illumina (New England BioLabs, Inc) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... were used to construct the RNA-seq libraries using a NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Entry vectors for Golden Gate cloning were made by digesting the Greengate Kit [56] entry vector plasmids (PGGA000, PGGB000, PGGC000, PGGD000, PGGE000, PGGF000) with BSA1-HF V2 (New England Biolabs Inc.). Bladderwort sequences were synthesized (IDT ...
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bioRxiv - Plant Biology 2021Quote: ... The pooled PCR reactions were purified with the Monarch® PCR & DNA Cleanup Kit (5 μg) (New England Biolabs® Inc.) and run on 6% acrylamide gel ...
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bioRxiv - Synthetic Biology 2021Quote: ... All the three fragments were assembled uisng the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, Ipswitch, MA, USA) and transformed into E ...
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bioRxiv - Systems Biology 2021Quote: ... Sequencing libraries were generated from the qualified DNA samples(s) using the NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated following manufacturer’s recommendations with NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA). The constructed libraries were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... (Leiden, The Netherlands) using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) and the NextSeq 500 platform (paired-end ...
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bioRxiv - Molecular Biology 2020Quote: ... ATG-free versions of the truncated OpIE2 promoter sequence (tIE2 promoter with no ATG triplet in its sequence) were created by Q5 Site-Directed Mutagenesis (SDM) Kit (New England Biolabs-NEB, USA). Primers used to generate mutations were designed using the NEBaseChanger tool (NEB ...