Labshake search
Citations for New England Biolabs :
7801 - 7850 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Site-directed mutagenesis was performed using standard PCR-based mutagenic procedures with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... with C-terminal TEV, GFP, 7xHis, and StrepII (Thawani et al., 2020) using the Q5 Site Directed Mutagenesis Kit (NEB). All three constructs were expressed using the Bac-to-Bac Sf9 cell expression system (Invitrogen) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The gRNA product was purified using DNaseI digestion and a Monarch® RNA Cleanup kit following the manufacturer’s instructions (NEB). The gRNA was further quantified by NanoDrop.
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bioRxiv - Synthetic Biology 2024Quote: ... The double stranded products were mixed with IVT reagents from the HiScribe® T7 High Yield RNA Synthesis kit (NEB) and incubated at 37 °C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II Directional Library prep kit for Illumina in conjunction with the NEBNext poly(A) magnetic isolation module (New England Biolabs). Library quality was assessed using the Agilent 2100 Bioanalyzer instrument ...
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bioRxiv - Plant Biology 2024Quote: ... and cDNA libraries were generated using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA). Following the methods of de Figueiredo et al ...
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bioRxiv - Plant Biology 2024Quote: ... We prepared polyA-RNA sequencing libraries from 400 ng of total RNA input using the NEBNext Ultra II RNA library preparation kit (New England Biolabs). Libraries were sequenced at IMGM (Martinsried ...
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bioRxiv - Neuroscience 2024Quote: ... and libraries were constructed using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). The libraries were sequenced on an Illumina NovaSeq 6000 system at a depth of 20 million 150-bp paired-end reads per sample ...
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bioRxiv - Plant Biology 2024Quote: ... Equal volumes of each indexed amplicon reaction were pooled and subsequently gel-purified using the Monarch DNA Gel Extraction Kit (New England Biolabs). A single Illumina sequencing library was prepared using the xGen DNA Library Prep MC Kit (Integrated DNA Technologies ...
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bioRxiv - Physiology 2024Quote: ... poly(A)+ mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation kit (New England BioLabs Inc., Massachusetts, USA). Prior to first strand synthesis ...
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Positive impact of Type-A and -C response regulator genes on growth and panicle architecture in ricebioRxiv - Plant Biology 2024Quote: ... The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse-transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Molecular Biology 2024Quote: The UCE-containing intron of CRNDE was in vitro transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) and used to generate a standard curve with defined numbers of CRNDEUCE copies ...
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bioRxiv - Molecular Biology 2024Quote: EMSAs were performed using purified eIF6-3XFlag protein and RNA synthesized by in vitro transcription with the HiScribe T7 High Yield RNA Synthesis Kit (NEB). Primers used for amplification of UCE intron or UCE intron fragment templates are provided in Table S3 ...
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bioRxiv - Molecular Biology 2024Quote: ... starting from the T7 promoter region in the insert cassette using the HiScribe T7 High Yield RNA Synthesis Kit (NEB). Transcribed gRNA was cleaned using the RNA Clean and Concentrator kit (Zymo).
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bioRxiv - Molecular Biology 2024Quote: ... the nsp14-Amp-replicon DNA was ligated to the fragment containing the nsp14 mutations using Gibson Assembly Cloning Kit (NEB). This resulted in the generation of nsp14 mutants-replicons.
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bioRxiv - Molecular Biology 2024Quote: ... the DNA underwent end-repair and adaptor ligation using the NEBNext® Ultra™ II DNA Library Prep Kit (NEB). Following affinity purification using Dynabeads MyOne Streptavidin C1 beads (Invitrogen) ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... The templates for in vitro translation were designed on the basis of the DHFR control plasmid from the PUREexpress kit (New England Biolabs). The FLAG tag sequence and mutations were introduced to the folA N-terminus by two rounds of the whole-plasmid PCR with primers listed in Supplementary table 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... the third copy of the human ubiquitin gene with a 3’ 15-nt spacer (complementary to OK117 primer) was then introduced into the resulted PCR fragment using NEBuilder HiFi DNA Assembly Cloning Kit (NEB). To generate pJLS3-1033 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the fourth copy of the human ubiquitin gene was cloned into the resulted backbone using NEBuilder HiFi DNA Assembly Cloning Kit (NEB). To generate the pmeDPC-56 substrate ...
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bioRxiv - Molecular Biology 2024Quote: ... the second copy of the human ubiquitin gene with a 3’ 15-nt spacer (complementary to OK115 primer) was then introduced into the resulted PCR fragment using NEBuilder HiFi DNA Assembly Cloning Kit (NEB). The resulted pJLS3-562 was first PCR amplified using OK113/OK115 primers (Supplementary Table 2) ...
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bioRxiv - Molecular Biology 2024Quote: The wild-type Xenopus HA-USP37 open reading frame (ORF) was cloned by introducing the respective gBlocks (IDT) using NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the AsiSI (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... corresponding to 10 – 285 nt of the USP37 gene (IDT) and containing the indicated mutations was introduced using NEBuilder HiFi DNA Assembly Cloning Kit (NEB). For protein expression ...
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bioRxiv - Molecular Biology 2024Quote: All PCR reactions within a given library were then pooled equally and purified together with the Monarch PCR and DNA Cleanup Kit (T1030L, NEB). The purified PCR products were then library prepped with the NEBNext Ultra™ II DNA Library Prep Kit (E7103S ...
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bioRxiv - Molecular Biology 2024Quote: ... The purified PCR products were then library prepped with the NEBNext Ultra™ II DNA Library Prep Kit (E7103S, NEB) and indexed with NEBNext Multiplex Oligos for Illumina (E6440S ...
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bioRxiv - Molecular Biology 2024Quote: ... The mutant of EctolA with a TEV site insertion was prepared by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). EctonB was subcloned into a pACYCDuet-1 vector with a C-terminal TEV protease site tag followed by a streptag-II ...
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bioRxiv - Molecular Biology 2024Quote: ... The final HiC library was generated with 5 PCR cycles using the NEBNext Ultra II DNA Library Prep Kit and NEBNext Dual Index primers (NEB) for Illumina sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared for all samples using the NEB Next Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to supplier recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... The extracted total RNA was converted into first-strand cDNA using the Luna Script RT Super Mix Kit (New England Biolabs). Subsequent qPCR analyses were performed using a an Mx3000P QPCR System (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µg of RNA was reverse transcribed using the ProtoScript II Reverse transcriptase kit from New England Biolabs (NEB #M0368S) and primed with oligo dT ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... primers listed in Table S1) was performed using One step Luna Universal RT-qPCR kit (New England Biolabs, Ipswich, MA) using an Applied Biosystems QuantStudio 3 (Thermofisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and the rRNA-depleted RNA was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... PAM sites were mutated by PCR amplification using primers to edit the sequence followed by treatment with KLD enzyme kit (New England BioLabs).
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bioRxiv - Microbiology 2024Quote: ... fragmented by ultrasonication to an average size of ∼250 bp and prepared for sequencing using the NEB Next Ultra I kit (E7370L, NEB), with some modifications ...
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bioRxiv - Microbiology 2024Quote: ... The rRNA-depleted RNA was then prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) and sequenced on an Illumina NextSeq 550 platform ...
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bioRxiv - Microbiology 2024Quote: ... The full-length viral RNA was transcribed in vitro from the linearized JEV cDNA templates using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB), and different 5′ cap structures of the viral genome were obtained by the addition of m7G(5′)ppp(5′)A cap analog or G(5′)ppp(5′)A cap analog (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNAs targeting MITF were designed using SplashRNA (http://splashrna.mskcc.org/) and cloned into a Doxycycline-inducible vector (pRRL-Puromycin) using the Q5 Site-Directed Mutagenesis Kit (NEB, Cat. # E0554S) with minor modifications ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and SNAP-tagged mouse MOR (cloned from SSF-MOR, a gift from the Von Zastrow lab) were produced using Gibson Assembly Cloning Kits (NEB) by placing a SNAP tag between the signal sequence/FLAG tag and the N-terminus of the receptor ...
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bioRxiv - Cell Biology 2024Quote: ... the cDNA library was cleaned using the Monarch PCR & DNA Cleanup Kit (5 μg) (T1030L, New England Biolabs, MA, USA) using the 7:1 ratio of binding buffer:sample as per the manufacturer’s instructions and was eluted in 27.5 μL nuclease-free water ...
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bioRxiv - Biophysics 2024Quote: ... was obtained as previously described.2 Mutagenesis to produce additional isoforms and mutants was performed using the Q5 Site Directed Mutagenesis Kit (New England Biolabs). 1N4R tau was generated using by an initial round of mutagenesis with primers (5’-CTGAAGAAGCAGGCATTGG-3’ and 5’-CTTCCGCTGTTGGAGTGC-3’ ...
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bioRxiv - Genomics 2024Quote: ... The recovered DNA was end-repaired and ligated to Illumina indexed adapters using the NEBNext Ultra DNA Library Prep Kit for Illumina NEB) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1: NEB). The adapter-ligated DNA underwent 6 or 8 cycles of PCR amplification ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs), which enabled the creation of strand-specific RNA-seq libraries ...
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bioRxiv - Immunology 2024Quote: ... 1 ng RNA was used as input for the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB). Poly-A enriched libraries were sequenced in 75bp single-end mode on a Novaseq 6000 system (Illumina ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 µL of reaction mixture containing 1 µg of template was prepared for in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) and incubated overnight at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNAseq libraries were prepared using 150 ng of total RNA per sample with the NEBnext Ultra II Directional RNA library kit for Illumina (New England Biolabs (NEB); Ipswich ...
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bioRxiv - Cell Biology 2024Quote: ... A plasmid expressing HIV-1 Gag with an internal EGFP tag was generated using the NEB HiFi Assembly Kit (New England Biolabs). A lentiviral backbone containing a tetracycline-inducible promoter and a gene encoding rtTA was prepared by digesting the pCW57.1 plasmid (Addgene #41393 ...
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bioRxiv - Bioengineering 2024Quote: All mRNAs were generated via in vitro transcription (IVT) reactions using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Coding sequences were ordered as gBlocks (IDT ...
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bioRxiv - Immunology 2024Quote: ... and enriched mRNA was used as an input for the NEBNext Ultra II RNA Library Prep Kit (NEB, Cat#: E7770L), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and enriched mRNA was used as an input for the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs (NEB)) ...
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bioRxiv - Cell Biology 2024Quote: RNA used for polyA-selected library generation for mRNA sequencing was isolated with the Monarch Total RNA isolation miniprep kit (New England Biolabs). Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508 ...