Labshake search
Citations for New England Biolabs :
7851 - 7900 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... using the NEBNext Ultra II DNA Library Prep Kit and NEBNext Multiplex Oligos for Illumina (NEB; E7645 and E7335, respectively), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Illumina sequencing libraries were prepared by amplifying 50 µL of each cDNA sample with sequencing adapters (500 nM each library amplification primer, Supplementary Table 3) using the NEBNext Q5 High Fidelity 2x PCR Kit (NEB) under the following cycling conditions ...
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bioRxiv - Biochemistry 2024Quote: RNA for in vitro SHAPE/DMS-MaP was synthesized using the HiScribe T7 High Yield RNA Kit (New England Biolabs). 100 µL reactions were assembled according to manufacturer instructions and incubated at 37 °C overnight ...
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bioRxiv - Cancer Biology 2024Quote: FFPE-extracted RNA samples were reverse-transcribed and sequencing libraries prepared using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: The cDNA library was constructed using the NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, E7760S) according to the manufactory instruction with some modifications ...
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bioRxiv - Genomics 2024Quote: CUT&RUN libraries were prepared from the released DNA fragments using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). The libraries were sequenced using Illumina NovaSeq 6000 with paired-end 50 base pair (50-PE ...
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bioRxiv - Genomics 2024Quote: ... and 100 ng used to generate the final libraries using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... Site-directed mutagenesis of SOX10 5’UTR was performed using Q5® Site-Directed Mutagenesis Kit (New England Biolabs, #E0554) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... we used the NEB Next PPFE repair kit with Ultra II end prep reaction (New England Biolabs, Ipswich, MA, USA) under recommended conditions and Nanopore ligation sequencing kit SQK-LSK110 ...
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bioRxiv - Genomics 2024Quote: ... In the size selection we added 0.9X volume of bead suspension (magnetic SPRI beads, supplied with the kit, NEB, E7103S) to the fragmented DNA ...
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bioRxiv - Genomics 2024Quote: ... Preparation of cDNA libraries was performed using NEBNext Ultra II RNA Library Kit for Illumina (Cat# E7770L, New England Biolabs) following Section 4 of the respective manual ...
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bioRxiv - Genomics 2024Quote: ... Adapters were ligated using the Genomic DNA by Ligation kit (SQK-LSK109, Oxford Nanopore Technologies) and NEBNext Quick T4 DNA Ligase (E6056, NEB) followed by AMPure XP bead clean-up ...
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bioRxiv - Microbiology 2024Quote: ... TruSeq indexed and universal adapters (Integrated DNA Technologies) were ligated to sample fragments using the Quick Ligation Kit (New England Biolabs). DNA fragments were separated on a 2% agarose gel and fragments from 600-800 base pairs were isolated using the QIAquick Gel Purification Kit (Qiagen) ...
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bioRxiv - Genetics 2024Quote: ... Proceeded to DNA library prep using 25ng of input and following the standard protocol for the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). During the PCR amplification ...
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bioRxiv - Genomics 2024Quote: ... gDNA was isolated from 160mg of bulk LIBD75 frontal cortex tissue using the Monarch HMW DNA Extraction Kit for Tissue (T3060S, NEB) following the manufacturer’s instructions with the following changes to the lysis step ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting sequencing libraries were assessed on an Agilent 2100 Bioanalyzer with a DNA 1000 chip and quantified through real-time PCR using the NEBNext Library Quant kit for Illumina (New England Biolabs). Raw sequencing data were generated on a NextSeq500 platform (Illumina ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from murine following the manufacturer’s instructions on Monarch® Total RNA Miniprep Kit (T2010S, New England Biolabs). Extracted RNA was quantified with Thermo Scientific™ NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer ...
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bioRxiv - Genomics 2024Quote: ... we added 10 uL of in vitro transcription mixture using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB), containing 1.5 uL of each NTP ...
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bioRxiv - Genomics 2024Quote: ... 2 µL of 10X T4 Ligase Buffer and 1 µL of NEB Golden Gate Assembly Kit (NEB, catalog no. E1602L) with 65 cycles of digestion at 42°C and ligation at 16°C for 5 min each ...
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bioRxiv - Genomics 2024Quote: ... subtilis 168 genomic DNA and inserted into pDG1662 via HiFi Assembly (all enzymes and reaction kits used in cloning were purchased from NEB, and high-fidelity versions of restriction enzymes were used if available) ...
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bioRxiv - Genomics 2024Quote: ... End-repair and A-tailing were performed with the End Prep module of the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The library preparation was performed using one reaction of NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645). We used the diluted adaptor (1.5 μM ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA underwent shearing to ∼200 bp fragments via sonication and the gDNA fragments were prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Bead-based size selection was employed to enrich for 200 bp fragments and the fragments then underwent a 17-cycle splinkerette PCR using oAM055 as the forward primer and either oAM068 (control ...
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bioRxiv - Biochemistry 2024Quote: ... Genomic DNA underwent shearing to ∼200 bp fragments via sonication and the gDNA fragments were prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Bead-based size selection was employed to enrich for 200 bp fragments and the fragments then underwent a 20-cycle splinkerette PCR using a Tn5-enrihcing forward primer (oAM55 ...
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bioRxiv - Plant Biology 2024Quote: Library preparation was made using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs); NEBNext® Poly(A ...
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bioRxiv - Synthetic Biology 2024Quote: ... while those from pM probes (T2A-fp) were isolated using the DNA Gel Extraction Kit (New England Biolabs, Cat# T1020L) to serve as the insert ...
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bioRxiv - Systems Biology 2024Quote: Single and double cysteine mutants were generated by site-directed mutagenesis were generated using Q5 Site-Directed Mutagenesis Kit (New England Biolabs). The all-cysteine mutant constructs were cloned by using customized synthesized gBlock (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... at 37 °C for 20 minutes before being purified using a Monarch RNA purification kit using 2X volume ethanol (New England Biolabs). Concentration determined using a Nanodrop ND-1000 ...
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bioRxiv - Bioengineering 2024Quote: circRNA was synthesized by IVT using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and the circRNA-synIRES-R25-NanoLuc DNA templates ...
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bioRxiv - Bioengineering 2024Quote: The in vitro synthesis of proteins was performed using the PURExpress In vitro Protein Synthesis kit (E6800, New England Biolabs). Four different sequences ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by column clean-up using the Monarch RNA Cleanup kit according to the manufacturer’s protocol for whole transcriptome isolation (New England Biolabs, T2030S). RNA quality was checked using an Agilent 2100 Bioanalyzer ...
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bioRxiv - Biophysics 2024Quote: All mRNAs used in these studies were transcribed from plasmids with the HiScribe T7 Quick High Yield RNA Synthesis Kit (E2050S, New England Biolabs). Transcripts contained a T7 promoter sequence (5’ – TAA TAC GAC TCA CTA TAG GG – 3’ ...
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bioRxiv - Biochemistry 2024Quote: ... Run-off transcription was then performed using 5 ng of PCR amplified template (HiScribe T7 High Yield RNA Synthesis kit; NEB), followed by DNase treatment (TURBO DNase ...
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bioRxiv - Genetics 2024Quote: ... This oligo may then be directly cloned into BplI digested pBHM2644-6 using an NEB HiFi DNA Assembly Kit (#E2621, New England Biolabs) per manufacturer’s instructions to bridge dsDNA with a ssDNA oligo.
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bioRxiv - Biophysics 2024Quote: ... and linear 7SK SL1 were transcribed from plasmid DNA templates prepared with QIAGEN Plasmid Maxi Kit and linearized with BamHI-HF restriction enzyme (New England Biolabs). Transcription mixtures are the same as the short RNA constructs above ...
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bioRxiv - Cancer Biology 2024Quote: ... dCas9-VPR mRNA was transcribed from this template using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s recommended protocol for high-yield synthesis with the following changes ...
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bioRxiv - Bioengineering 2024Quote: ... Purified templates were used in 20 μL T7 in vitro transcription reactions using the HiScribe T7 in vitro transcription kit (New England Biolabs) at 100 nM ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and SNAP-tagged mouse MOR (cloned from SSF-MOR, a gift from the Von Zastrow lab) were produced using Gibson Assembly Cloning Kits (NEB) by placing a SNAP tag between the signal sequence/FLAG tag and the N-terminus of the receptor ...
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bioRxiv - Bioengineering 2024Quote: ... for 15 min at 37°C and immediately purified using the Monarch® RNA Cleanup Kit (New England Biolabs, USA). RNA samples were diluted to the desired working concentration and stored at -80 °C prior to use.
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bioRxiv - Cell Biology 2024Quote: RNA used for polyA-selected library generation for mRNA sequencing was isolated with the Monarch Total RNA isolation miniprep kit (New England Biolabs). Libraries were constructed using the Nugen Universal Plus mRNA-SEQ library construction kit (Nugen 0508 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 µL of reaction mixture containing 1 µg of template was prepared for in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) and incubated overnight at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNAseq libraries were prepared using 150 ng of total RNA per sample with the NEBnext Ultra II Directional RNA library kit for Illumina (New England Biolabs (NEB); Ipswich ...
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bioRxiv - Cell Biology 2024Quote: ... A plasmid expressing HIV-1 Gag with an internal EGFP tag was generated using the NEB HiFi Assembly Kit (New England Biolabs). A lentiviral backbone containing a tetracycline-inducible promoter and a gene encoding rtTA was prepared by digesting the pCW57.1 plasmid (Addgene #41393 ...
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bioRxiv - Bioengineering 2024Quote: All mRNAs were generated via in vitro transcription (IVT) reactions using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Coding sequences were ordered as gBlocks (IDT ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNAs targeting MITF were designed using SplashRNA (http://splashrna.mskcc.org/) and cloned into a Doxycycline-inducible vector (pRRL-Puromycin) using the Q5 Site-Directed Mutagenesis Kit (NEB, Cat. # E0554S) with minor modifications ...
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bioRxiv - Cancer Biology 2024Quote: Cells growing in monolayer cultures were directly harvested in RNA lysis buffer and processed with the Monarch RNA purification kit (NEB). cDNA was synthesized with the LunaScript RT SuperMix kit (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was extracted from subconfluent cells and treated with DNase I with the Monarch total RNA purification kit (NEB). Reverse transcription and PCR amplification were performed with the Luna One-Step RT-qPCR kit (NEB) ...
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bioRxiv - Biophysics 2024Quote: ... L258D and additional point mutations were introduced by site directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the cDNA library was cleaned using the Monarch PCR & DNA Cleanup Kit (5 μg) (T1030L, New England Biolabs, MA, USA) using the 7:1 ratio of binding buffer:sample as per the manufacturer’s instructions and was eluted in 27.5 μL nuclease-free water ...
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bioRxiv - Biophysics 2024Quote: ... was obtained as previously described.2 Mutagenesis to produce additional isoforms and mutants was performed using the Q5 Site Directed Mutagenesis Kit (New England Biolabs). 1N4R tau was generated using by an initial round of mutagenesis with primers (5’-CTGAAGAAGCAGGCATTGG-3’ and 5’-CTTCCGCTGTTGGAGTGC-3’ ...