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Citations for New England Biolabs :
7901 - 7950 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Bulk RNA sequencing of K562 cells at 37°C was conducted by generating a cDNA library prepared by depleting rRNA using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) and then the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... Truncations and modifications of the stalling construct were produced by deletion PCR using a Q5-SDM kit or Gibson assembly (New England Biolabs). DNA for transfection was prepared using PureLink™ HiPure Plasmid Maxi-prep kit (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: The TSV-IRES mRNA 6741-6990 was obtained by T7 in-vitro transcription from a PCR product amplified from the plasmid containing the mRNA clone and the transcript was purified by using RNA purification kit (NEB) (4).
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bioRxiv - Biochemistry 2024Quote: ... After 30 min of incubation at 37 °C the products of digestion were analyzed with PAGE directly or isolated from the reaction mixture using Monarch RNA cleanup kit (10 µg, NEB).
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bioRxiv - Bioengineering 2024Quote: ... were mixed with 0.5 µL of NEBridge Golden Gate Assembly Kit (BsaI-HF v2) reaction mix (New England Biolabs E1601), 1 µL of T4 DNA ligase buffer ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments were subject to end repair and dA-tailing with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), ligated to Ad1 at both ends and denatured to be incubated with CPD antibody-coated beads ...
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bioRxiv - Biochemistry 2024Quote: ... The CreΔ330 truncation mutant was constructed by introducing a TAG stop codon following residue 330 using the Q5 Site directed mutagenesis kit (NEB) and primers 5’-CCTGGATAGTtagACAGGGGCAATG-3’ and 5’-TTACGGATATAGTTCATGAC-3’ ...
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bioRxiv - Biochemistry 2024Quote: ... The P105TAG-DHFR DNA template containing a stop codon at position 105 was mutated from the DHFR-WT DNA using the Q5 site-directed mutagenesis kit (NEB).
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bioRxiv - Cancer Biology 2024Quote: ... We quantified the eluted DNA using a Qubit fluorometer and prepared sequencing libraries using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Both first-strand complementary DNA (cDNA) synthesis and RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in 384-well plates using a QuantStudio 7 Flex (Life Technologies ...
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bioRxiv - Biochemistry 2024Quote: ... These sequences were synthesized with 20 bp overhangs and cloned into the pEAQ-HT vector using Gibson assembly (NEBuilder hi-fi assembly kit, New England Biolabs) after AgeI/XhoI digestion and gel purification (42) ...
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bioRxiv - Biochemistry 2024Quote: ... LRRK2 mutant constructs were generated by site-directed mutagenesis using the Q5 site-directed mutagenesis kit from New England Biolabs (#E0552S, NEB). All plasmids were maintained in E ...
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bioRxiv - Biochemistry 2024Quote: ... The mutant constructs of PolXa□Nter were generated via site-directed mutagenesis performed by PCR (primers are indicated in Supplementary Table S2) and using the KLD enzyme kit (New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... total RNA-seq libraries were generated using the NEBNext Ultra II Directional RNA Library prep kit for Illumina with multiplex oligos set 1 (NEB) (GEO ...
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bioRxiv - Biochemistry 2024Quote: ... All RNAs were transcribed with T7 RNA polymerase in 40-60 μl reactions with HiScribe T7 High Yield RNA Synthesis Kit (NEB). The in vitro transcription reaction was performed for 5 hours at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... RNAs were cleaned and eluted using Monarch® RNA Cleanup Kit (Catalog no. T2050L, New England Biolabs Pte. Ltd., Singapore), following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... Additional mutations were cloned into BE plasmids using isothermal assembly the Q5 Site-Directed Mutagenesis Kit (E0554; New England Biolabs; NEB). Expression plasmids for human U6 promoter-driven gRNAs were generated by annealing and ligating duplexed oligonucleotides corresponding to spacer sequences into BsmBI-digested pUC19-U6-BsmBI_cassette-SpCas9_gRNA (BPK1520 ...
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bioRxiv - Biochemistry 2024Quote: ... The T1D-protective mutations were introduced into the MDA5-ΔL2 construct with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs).
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bioRxiv - Bioengineering 2024Quote: The IVT reactions were set up using the HiScribe T7 High-Yield RNA Synthesis Kit (New England Biolabs, Cat #E2040S), modified with full pseudo-UTP substitution using N1-Methyl-Pseudo-U (TriLink Biotechnologies ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries for RNA sequencing were prepared using the Illumina NEBNext Ultra II Directional RNA Library Prep Kit with the NEBNext rRNA Depletion Module (New England Biolabs). RNA libraries were sequenced on the Illumina HiSeq system with 250 cycles ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were purified by DNA agarose gel electrophoresis and subsequent DNA extraction using Monarch DNA Gel Extraction Kit (New England Biolabs (NEB)).
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bioRxiv - Cell Biology 2024Quote: ... Following in vitro transcription at 37°C overnight using the HiScribeTM T7 High Yield RNA Synthesis Kit (New England Biolabs (NEB)) ...
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bioRxiv - Bioengineering 2024Quote: ... overlapping fragments were amplified by PCR and the PCR products and the digested plasmid were assembled using Gibson Assembly kit (New England Biolabs) to obtain the resulting plasmid.
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bioRxiv - Bioengineering 2024Quote: ... Additional mutations were cloned into SpCas9-VRQR nuclease or ABE plasmids using isothermal assembly the Q5 Site-Directed Mutagenesis Kit (E0554; New England Biolabs; NEB). Expression plasmids for human U6 promoter-driven gRNAs were generated by annealing and ligating duplexed oligonucleotides corresponding to spacer sequences into BsmBI-digested pUC19-U6-BsmBI_cassette-SpCas9_gRNA (BPK1520 ...
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bioRxiv - Cancer Biology 2024Quote: ... The purified mRNA was used for library preparation using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, US) using the protocol provided in the kit ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA libraries of ChIP-Rx samples were prepared by using the NEBNext Ultra II DNA Library Prep Kit for Illumina Sequencing (New England Biolabs).
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bioRxiv - Cancer Biology 2024Quote: ... On-column DNase I digestion was performed followed by mRNA isolation with the NEBNext Poly (A) mRNA Magnetic Isolation Kit (NEB). Library preparation was done with the Ultra II Directional RNA Library Prep for Illumina following the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2024Quote: ... was used for preparation of DNA extracted from the bulk samples whilst the protocol for microdissected tissue used the NEBNext Ultra II DNA FS Library Prep Kit (New England Biolabs) instead ...
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bioRxiv - Cancer Biology 2024Quote: ... Novogene used NEBNext® Ultra RNA Library Prep Kit for Illumina® (cat# E7420S, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: A promoter insertion plasmid was generated by cloning three PCR fragments into a modified pCR2.1-TOPO vector using Gibson Assembly Cloning Kit (NEB #E5510). One fragment corresponding to the TgSERCA flanking region (predicted promoter/5’UTR ...
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bioRxiv - Cancer Biology 2024Quote: ... The generation of dual-indexed libraries for WGBS followed a modified protocol based on the NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs), in accordance with the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... RNA-seq libraries were prepared in two biological replicates using NEBNext Ultra II Directional RNA Second Strand Synthesis Module and NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) as per manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... We extracted and purified the DNA in the 400 to 600 bp region using the Monarch Gel Extraction Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... a whole genome shotgun library was generated with ∼500 ng total genomic DNA using the NEBNext Ultra II kit (New England Biolabs). Prior to library preparation ...
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bioRxiv - Genetics 2024Quote: ... The coding sequence of each gene was amplified by RT-PCR on adult testis cDNA preparations and cloned into Y2H/Y3H vectors using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The specific alleles of each gene family member used in this study are provided in Supp ...
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bioRxiv - Genetics 2024Quote: ... Two micrograms of whole mosquito-derived or 200ng of hemolymph-derived total RNA were used for first-strand synthesis with the LunaScript RT SuperScript Kit (NEB). Gene expression analysis was performed with quantitative real-time PCR (qPCR ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using purified mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7765L) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µg was reverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit with oligo-dT primers and murine RNase inhibitor (New England Biolabs). Quantitative PCR was performed in technical triplicate using 20 ng cDNA ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit (New England BioLabs, Ipswich, MA, USA), and 2 x 150 bp paired-end sequencing (Q30 > 85% ...
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bioRxiv - Genetics 2024Quote: ... 500 ng of Total RNA was utilized as the input for sequencing library construction with the NEB Next small RNA library prep kit (New England Biolabs), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: The mRNAs for cell-free protein expression were transcribed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Libraries were generated using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Sequencing was performed in the NovaSeq 6000 System (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... an in-tube treatment with DNase I was performed using Monarch® DNase I and RNA was purified using the Monarch® RNA Cleanup Kit according to manufacturer’s instructions (New England Biolabs). Isolated RNA (0.5 - 1 μg ...
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bioRxiv - Evolutionary Biology 2024Quote: ... cDNA libraries were constructed using the NEBnext Ultra II Directional RNA Library Preparation Kit with polyA selection and dUTP method (New England BioLabs) and sequenced on an Illumina NovaSeq 2×150bp ...
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bioRxiv - Cell Biology 2024Quote: ... using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the polyA mRNA workflow (New England Biolabs). Samples were sequenced using a NovaSeq6000 (Illumina) ...
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bioRxiv - Cell Biology 2024Quote: ... using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the rRNA depletion workflow (New England Biolabs). cDNA libraries were constructed for the intermediate stiffness time course (comprising stiff ...
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bioRxiv - Cell Biology 2024Quote: α-Synuclein-A140C was generated from the pT7-7 aSyn WT vector using the Q5 Site-Directed Mutagenesis Kit (NEB, E0554) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... the libraries for RNA sequencing were generated from fragmented DNA with eight cycles of amplification using a NEB Next Ultra DNA Library Prep Kit (New England Biolabs). After the libraries were quantified using the Bioanalyzer (Agilent) ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (E7420L, New England Biolabs, Massachusetts, USA), followed by sequencing on an Illumina Hiseq 4000 system ...
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bioRxiv - Evolutionary Biology 2024Quote: ... of which 5 μl was processed with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) with published modifications to the manufacturer’s protocol (Batty et al ...