Labshake search
Citations for New England Biolabs :
7651 - 7700 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... NEBNext Ultra II DNA Library Prep Kit for Illumina with NEBNext Multiplex oligos for Illumina (NEB cat # E7645S/L; E6440) following the instruction manual (V6.1_5/20 ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared from rRNA depleted samples using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on the Novaseq 6000 S4 (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: Visible LEAP bands were excised from agarose gels and cDNAs were purified using the Monarch DNA Gel Extraction Kit (NEB). Purified DNAs from excised gel slices were cloned into a pCR4Blunt-TOPO vector using a Zero Blunt TOPO PCR Cloning Kit for Sequencing (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... ΔPYD and HD mutants were generated from full length IFI207-HA plasmids and the R1-ΔPYD mutant from R1-IFI207 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). IFI207-HIN plasmids were generated by PCR amplification of the HIN domain from full length IFI207-V5 expression plasmids ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: Codon-optimized full-length SARS-CoV-2 S protein gene was cloned into Topo cloning vector and 22 N-glycosylation sites were mutated individually from N to Q using a Q5 Site-Directed Mutagenesis Kit (NEB). The full-length or C19 truncated fragments were amplified and inserted into plvx-puro vector ...
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bioRxiv - Microbiology 2024Quote: ... the construct generated above with the WT rel allele in pIMAY (pRel-swap, Table S2) was used for site directed mutagenesis with the Q5-Site-Directed-Mutagenesis kit (NEB) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... and its variant CjeNThr69Ser77Thr111his6 were cloned into pET21a(+) plasmid using a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs). The proteins were overexpressed in E ...
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bioRxiv - Molecular Biology 2024Quote: ... and Rpa2WH (Rpa2178-268) were cloned from the pRSFduet(+) constructs using the Q5 site directed mutagenesis kit (E0554, New England Biolabs).
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bioRxiv - Microbiology 2024Quote: ... the RNA pellet was resuspended in 50 µl of RNase-free H2O and 1 µl of the resuspended samples was used for quantification of the DENV-2 D220 NS5 RNA regions using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in a 10-ul reaction volume ...
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bioRxiv - Microbiology 2024Quote: ... mutagenic primers were designed using the New England Biolabs online software NEBaseChanger (primer sequences are available at doi: XXXX. The Q5 Site-Directed Mutagenesis Kit (New England BioLabs) was then used with the A/Baltimore/R0675/2019 hemagglutinin plasmid to produce plasmids with the desired mutations ...
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bioRxiv - Neuroscience 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was performed using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S/L). The workflow was adapted to generate longer library fragments by reducing the fragmentation time (see manual ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3′ adapter was conjugated with an amino CA linker instead of dCC at the 3′ end (GeneDesign) and then adenylated at the 5′ end with a 5′ DNA adenylation kit (NEB). Four random nucleotides were added in the 3′ and 5′ adapters [(5′ -rAppNNNNTGGAATTCTCGGGTGCCAAGG/amino CA linker-3′ ...
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bioRxiv - Molecular Biology 2024Quote: ... These three DNA fragments were further assembled using the NEBuilder HiFi DNA Assembly cloning kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Molecular Biology 2024Quote: ... DNA fragments were subject to end-repair and dA-tailing with NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs), followed by ligation with 50 pmol of adaptor 1 (Ad1 ...
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bioRxiv - Microbiology 2024Quote: ... This dimer was introduced into pBECAb-apr using the NEBridge Golden Gate Assembly Kit (New England Biolabs, Ipswich, MA, USA) to generate pBECAb-apr-kmtA ...
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bioRxiv - Molecular Biology 2024Quote: ... Modifications of uORFs or other mutations were performed using the Q5 site-directed mutagenesis kit (Cat. No. E0554S, New England Biolabs) and validated by Sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP-seq libraries were generated using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB # E7645S) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 cycles on a Covaris E220 focused ultrasonicator and were subsequently processed using NEBNext Enzymatic Methyl-Seq kit (NEB #7120) following the manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid pHERD30t with and without overlapping AluI/BREX site was purified from BREX+ (E. coli HS) or BREX- cells (E. coli BW25113) using Monarch Plasmid Purification Kit (NEB). 100 ng of plasmid was restricted with AluI (ThermoScientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Introduction of the overlapping BREX/AluI site to the pHERD30t vector was also achieved via Q5 site-directed mutagenesis kit (NEB). Plasmids were transformed into chemically competent XL1-Blue cells (Evrogen) ...
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bioRxiv - Molecular Biology 2024Quote: 50-100 ng of purified PCR product was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: 175-225 ng of purified PCR product was prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: 200 ng of pooled PCR product was prepared for sequencing using the NEB-Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Genomics 2024Quote: ... libraries were generated from 250 ng of DNase-treated RNA with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the Poly(A ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was converted into Illumina-compatible libraries with the NEBNext(R) Ultra(TM) II DNA Library Prep Kit (NEB E7645L) following the protocol described by 41 and using NEBNext Dual Index Multiplex Oligos (NEB E7600S) ...
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bioRxiv - Genetics 2024Quote: ... with constructs cloned in place of GFP via the Gibson assembly method using the NEBuilder HiFi Cloning Kit (NEB, #E5520S). GPS lentiviral vectors encoding the N-terminus of PTGS1 and the C-terminus of TNNC2 fused to GFP were gifts from Stephen Elledge ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription was performed with 1 µg of RNA using one-step reaction using LunaScript RT SuperMix Kit (NEB, #E3010) as indicated by the manufacturer ...
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bioRxiv - Synthetic Biology 2024Quote: ... where separately introduced to the wild-type MS2 coat protein present in these mRNA constructs using the NEB-Q5 mutagenesis kit (New England Biolabs). The primer sets and PCR settings used are listed in supplementary information ...
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bioRxiv - Synthetic Biology 2024Quote: ... These synthesized fragments were assembled into the Level 0 entry vector pYTK001 using the NEBridge Golden Gate Assembly Kit (BsmBI-v2) (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... rRNA-depleted RNA was used to generate the RNA-sequencing libraries using NEB Ultra II directional kit (New England Biolabs). Samples from Keratinocytes were multiplexed and sequenced on a HiSeq4000 PE 150 sequencing platform (Illumina) ...
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bioRxiv - Genetics 2024Quote: Golden Gate Assembly was performed largely according to the manufacturer protocol included with the NEBridge Golden Gate Assembly kit (New England Biolabs) using the with alterations noted below (Potapov et al ...
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bioRxiv - Genomics 2024Quote: ... 1µg RNA was used for first strand cDNA preparation by using ProtoScript II Reverse Transcriptase kit followed by Phusion High-Fidelity DNA Polymerase (NEB). RT-qPCR was performed with LunaScript RT Supermix (NEB ...
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bioRxiv - Zoology 2024Quote: ... The transcriptome sequencing libraries were prepared with poly A selection using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and were sequenced using the NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... rRNA-depleted samples were prepared for next-generation sequencing using NEBNext Ultra II Directional RNA Library Prep kit (NEB; E7765S) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries for RNA-Seq were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760S), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... after extracting total RNA from the testis (three replicates per genotypes) using the Monarch total RNA mini prep Kit (New England Biolabs), we generated RNA-seq libraries using the NEBNext Rrna Depletion Kit (New England Biolabs ...
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bioRxiv - Immunology 2024Quote: ... Gene expression levels were quantified by reverse transcription quantitative PCR (RT-qPCR) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), and being conducted on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were then prepared from 20ng of input DNA using the NEBNext Ultra-II DNA kit (New England Biolabs) following manufacturer’s instructions22 ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, Germany). The size distribution of the libraries was checked by high-resolution gel electrophoresis with a Bioanalyzer instrument using the DNA 7500 Pico kit (Agilent Technologies) ...
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bioRxiv - Genomics 2024Quote: ... was used as the original tissue sample for genomic DNA extractions with Monarch High Molecular Weight (HMW) Extraction Kit for Tissue (New England Biolabs). Spleen tissue (totaling 25 mg ...
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bioRxiv - Genomics 2024Quote: Double-stranded DNA libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolab: NEB). Around 1 ng of DNA was used for each library ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were generated using the NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Microbiology 2024Quote: Approximately 1×107 bacterial cells were centrifuged at 12,000 x g for 1 minute before beginning RNA extraction as per the Monarch Total RNA Miniprep kit protocol (New England Biolabs). RNA was eluted in 30 μL nuclease-free water (Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... The PCR products from divergent primers of expected length were excised and purified via PCR clean up kit (New England Biolabs) for confirmation of BSJ using Sanger Sequencing.
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bioRxiv - Genetics 2024Quote: ... the Cas9 terminator (TCYC1) was switched to an ADH1 terminator (TADH1) by Gibson assembly using the HiFi DNA Assembly kit (New England Biolabs). Cas9D10A ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from libraries was prepared according to the instructions of the “NEBNext Ultra Directional RNA Library Prep kit for Illumina” (New England Biolabs), following the “Poly(A ...
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bioRxiv - Immunology 2024Quote: ... 100 ng of the WTA library was subjected to fragmentation/end-repair/A-tailing using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Purified adapter-ligated products were subjected to index PCR ...
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bioRxiv - Molecular Biology 2024Quote: Libraries of immunoprecipitated DNA were generated from 3 ng of starting DNA with the NEBNext Ultra DNA Library Prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions at the CRG Genomics Core Facility (Barcelona ...