Labshake search
Citations for New England Biolabs :
7201 - 7250 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... After DNA isolation libraries were prepared with the NEBNext ULTRA II DNA Library Prep Kit for Illumina (NEB, US) according to manufacturer’s specification with the NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: We prepared the DNA extracts into sequencing libraries following the NEBNext Ultra II FS DNA Library Prep Kit (NEB) according to manufacturers’ instructions but replaced the NEBNext Adapters with Y-Adapters ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and gel excision of DNA between 150 bp and 1,500 bp was performed using the Monarch DNA Gel Excision Kit (NEB). Concentrations of the purified libraries were measured using PicoGreen (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: Small RNA libraries from immunoprecipitated RNAs or total RNA were prepared using Small RNA Library Prep Kit (E7300, NEB) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified DNA was inserted into the Hsp68-lacZ vector using the Gibson Assembly kit (E2611S, New England Biolabs). The resulting constructs were verified ...
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bioRxiv - Developmental Biology 2024Quote: ... and both reverse transcription and PCR were performed using the Luna One-Step Universal RT-qPCR kit (NEB, E3005S) and carried out on the Mic qPCR Machine (BioMolecular Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... and mRNA was transcribed in vitro from the templates using HiScribe T7 ARCA mRNA Kit with Tailing (NEB, E2060) or mMESSAGE mMACHINE SP6 Transcription Kit (Thermo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were constructed with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, #E7760S) and sequenced on an Illumina NextSeq2000 platform to generate a minimum of 25-30 million 150-bp paired-end reads per sample.
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bioRxiv - Genomics 2024Quote: ... Poly-A selected libraries were prepared with a NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced using the NovaSeq 6000 (Ilumina ...
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bioRxiv - Genomics 2024Quote: Methylation enrichment of cfDNA was performed using the EpiMark Methylated DNA Enrichment Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and p.V126V were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs, Ipswich, MA; catalog no. E0552). Primers used for site-directed mutagenesis are given in Appendix 1-table 7 ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645S) with the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... and prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) according to the manual ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation was performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs; USA) with 1 μg total RNA per sample ...
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bioRxiv - Immunology 2024Quote: ... the drh-1(2CARD) sequence was deleted from pET770 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Primers LEB003 and LEB004 were used to amplify around the drh-1(2CARD ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were then either purified directly using Monarch PCR and DNA Cleanup Kit (New England Biolabs, Hitchin, UK), or if more than one band was present extracted from 1% TAE gel using the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... 25 °C) using a custom methionine(-) Flexible In vitro Translation system composed by PURExpressTM (ΔRF123) Kit (New England Biolabs) solution B ...
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bioRxiv - Molecular Biology 2024Quote: ... Library preparation was prepared after polyA mRNA selection using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB). Sequencing was performed on Illumina NovSeq600 using a 2×150 paired end configuration ...
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bioRxiv - Microbiology 2024Quote: ... double-stranded cDNA libraries were generated by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Libraries for sequencing were prepared using extracted RNA and the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (New England Biolabs). Quality of all RNA and library preparations were evaluated with BioAnalyzer 2100 (Agilent).
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... and HCN4 Δ1-185 deletion clones were made using a site-directed mutagenesis kit (New England Biolabs, Ipswich, MA) and a codon-optimized HCN4 plasmid in the pTwist-CMV-WPRE-Neo vector synthesized by Twist Biosciences.
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs). RNA libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs). For both types of libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries for RNA-seq were made with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation and multiplexing for ChIP- seq libraries were performed using the NEBNext ChIP-seq library Prep kit (NEB). The sequencing of ChIP-seq and input control libraries was performed using Illumina HiSeq-2500.
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bioRxiv - Immunology 2024Quote: Bulk RNA sequencing libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) and sequenced on a high throughput NextSeq 500 device ...
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bioRxiv - Synthetic Biology 2023Quote: ... In vitro transcription of sgRNAs was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Then ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB, E6420S) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra™ DNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: Libraries in this paper were prepared using NEB’s rRNA Depletion Kit (Bacteria) for rRNA removal with beads (NEB #E7860). Post rRNA removal samples were prepared for either Illumina or Singular Sequencing using NEBNext Ultra II RNA Library Prep Kit for Illumina with Beads (NEB#E7775) ...
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bioRxiv - Genomics 2023Quote: ... Linearized destination vectors were gel purified using 0.7% agarose gels and extracted with the Monarch DNA Gel Extraction Kit (New England Biolabs), before further purification by isopropanol precipitation.
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bioRxiv - Genomics 2023Quote: ... The remaining thirteen samples had paired-end sequencing libraries constructed according to the manufacturer’s specifications for the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Three samples (RMF031 ...
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bioRxiv - Genomics 2023Quote: Sample DNA concentrations were quantified using Qubit and libraries prepared using NEBNext Ultra II DNA library preparation kit (NEB) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... About 1ng DNA was used for library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Sequencing (2× 150bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were prepared using the NEB Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, United States), and all samples were run on one lane of the NovaSeq6000 S4 v1.5 (Illumina ...
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bioRxiv - Genomics 2023Quote: ... or 100 cGy) was processed for bisulfite sequencing library construction using NEBNext Ultra II DNA Library Prep Kit (NEB) along with methylated and indexed NEXTflex Bisulfite-seq Barcodes (Bio scientific) ...
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bioRxiv - Genomics 2023Quote: ... Total (“steady-state”) RNA was converted to sequencing libraries using NEB polyA capture kit (product no) followed by NEB Ultra directional II RNA-seq library kits ...
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bioRxiv - Cancer Biology 2023Quote: ... An RNA-seq library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Isotope labeled firefly luciferase (Fluc) mRNA was synthesized by HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB, Ipswich) using stable heavy isotopes of ATP and CTP (NLM-3987-CA and CNLM-4267-CA-20 ...
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bioRxiv - Genetics 2024Quote: DNA from the VIU and MBP breeding program samples was extracted using the Monarch Genomic DNA Purification kit (NEB) using the enzymatic cleanup tissue extraction protocol with proteinase K and RNase A and quantified using a BioSpectrometer (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were prepared from 10 ng of RNA using the NEBNext Ultra kit (New England BioLabs, Ipswich, MA, USA) and sequenced on an Illumina HiSeq 4000 with a 75 bp paired-end read ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sheared DNA was then adapter-ligated (AL) with NEBnext Ultra ii Library Preparation kit (New England Biolabs, Ipswich, MA). AL-DNA quality was again confirmed by Bioanalyzer high sensitivity DNA kit (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA libraries were prepared and indexed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530L). Libraries were sequenced with the NovaSeq 6000 at a depth of 20 million paired-end 150 base pair reads per sample ...
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bioRxiv - Immunology 2022Quote: ... quantitative reverse transcription PCR (qRT-PCR) was conducted using Luna universal probe one-step RT-PCR kit (NEB, #E3006) on CFX96 Touch Real-Time Detection System on 96-well plates (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... The pRSLepB–CutF(SM10) and pRSLepB–CutF(SM28) were constructed by using the Q5 mutagenesis Kit (NEB Lab, MA) with cutF(AP 10&28)-F/ cutF(AP-10aa)-R and cutF(AP 10&28)-F/cutF(AP-28aa)-R mutagenic primer pairs carrying the 10 and 28 aa C-terminal proline-rich segment of CutF respectively (Fig ...
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bioRxiv - Molecular Biology 2022Quote: ... Sequencing libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB #E7760) coupled with AMPure XP bead purification (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... The library was generated using a NEBNext® Single Cell/Low Input RNA Library Prep Kit (New England Biolabs). RNAseq was performed using an Illumina NovaSeq 6000 ...
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bioRxiv - Biochemistry 2023Quote: ... Histone H2A chimeric mutant mimicking H2A.Z acidic patch (N94D/K95S) was generated using the Q5 mutagenesis kit (New England Biolabs). Monomethylated histone H4 lysine 20 (H4K20me1 ...