Labshake search
Citations for New England Biolabs :
7401 - 7450 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: 500 nanograms of total RNA were used for NEBNext® Ultra II Directional RNA Library Prep Kit (NEB # E7760L). Poly-A selection (NEBNext Poly (A ...
-
bioRxiv - Genomics 2023Quote: ... The remaining thirteen samples had paired-end sequencing libraries constructed according to the manufacturer’s specifications for the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB). Three samples (RMF031 ...
-
bioRxiv - Molecular Biology 2023Quote: ... pcDNA3.1-ATP6V1H(Δ176-191)-FLAG and pcDNA3.1-ATP6V1H(Δ176-193)-FLAG were generated using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). pENTR-ATP6V1H-FLAG was produced using the pENTR/D-TOPO Cloning Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... Site-specific mutagenesis of efp was carried out with the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... Replacement of the interchangeable sgRNA was accomplished using a Q5 site-directed mutagenesis kit (Table S3) (New England BioLabs). The TgLaforin-HA tagging construct was generated by amplifying the 3’ end of the TgLaforin-HA construct generated for complementation (see generation of COMP line below and Tables S2 and S3 ...
-
bioRxiv - Molecular Biology 2023Quote: Sequencing libraries were prepared using the NEBNext Ultra II DNA library prep kit according to the manufacturer protocol (NEB). Sequencing libraries were pooled and analyzed on a NextSeq 550 platform using paired-end mode with 32 cycles on both ends ...
-
PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... Libraries were constructed using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB, E6420S) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Libraries were prepared using the NEB Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, United States), and all samples were run on one lane of the NovaSeq6000 S4 v1.5 (Illumina ...
-
bioRxiv - Developmental Biology 2023Quote: ... About 1ng DNA was used for library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB). Sequencing (2× 150bp ...
-
bioRxiv - Neuroscience 2023Quote: ... deposited by Bischof and Basler) at the EcoRI restriction site by NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S). The pUASTattB-Gs2 vector was injected to y1 w67c23 ...
-
bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2023Quote: Mutant variants of the mS29-containing construct were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). Mutagenesis primers were designed using the NEBaseChanger tool ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... using the Monarch® HMW DNA Extraction Kit for Tissue (Cat. No. T3060S, New England BioLabs Inc., MA, USA). DNA from resequencing samples was isolated using either a modified protocol for the DNeasy Blood and Tissue Kit (Cat ...
-
bioRxiv - Genetics 2023Quote: ... which was immediately used as a template for seven synonymous single-nucleotide substitutions into the terminal 21bp of the ZK938.12 gene fragment by Q5 site-directed mutagenesis kit (NEB). The resultant plasmid was named pDSP47.
-
bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA and quantitative PCR was performed using the Luna Universal One-step RT-qPCR Kit (NEB) using 1 µg of RNA per reaction ...
-
bioRxiv - Molecular Biology 2023Quote: ... The gel purified DNA fragments were cloned into the pMiniT 2.0 vector using the NEB PCR Cloning Kit (New England BioLabs) and then transformed into NEB 10-beta competent cells (New England BioLabs) ...
-
bioRxiv - Microbiology 2023Quote: ... and directional cDNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). cDNA library quality was assessed using a High Sensitivity DNA Kit (Agilent) ...
-
bioRxiv - Molecular Biology 2023Quote: ... T7 transcription was performed for 16 h and then RNA was purified using the Monarch RNA Cleanup Kit (NEB). In vitro cleavage was performed with purified recombinant ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB). The size of the resulting library was controlled by use of a D1000 ScreenTape (Agilent 2200 TapeStation ...
-
bioRxiv - Microbiology 2023Quote: 20 ng of nucleic acid was enriched for viral genomic sequences using NEBNext Microbiome DNA Enrichment Kit (NEB, E2612) to reduce host genomic DNA contamination ...
-
bioRxiv - Plant Biology 2023Quote: ... and each individual library was barcoded using the NEBNext® Multiplex Oligos for Illumina® kit (New England Biolabs).
-
bioRxiv - Cancer Biology 2023Quote: ... and PCR enrichment were performed sequentially using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB). Lastly ...
-
bioRxiv - Synthetic Biology 2024Quote: ... then inserted the library cassette into the digested pMPRA1 backbone using the NEBuilder HiFi DNA Assembly kit (NEB E2621). This intermediate plasmid was purified with Kapa Pure Beads (Roche KK8002) ...
-
bioRxiv - Genetics 2024Quote: ... Libraries were constructed with NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA).
-
bioRxiv - Cell Biology 2024Quote: ... The pooled library was once again bead purified and quantified by qPCR using NEBNext Library Quant Kit (NEB, E7630). The library size was verified using a High Sensitivity DNA Kit on a 2100AB Bioanalyzer instrument (Agilent) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The RNA-sequencing (RNA-seq) libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420L), The yield of cDNA libraries was measured using the Qubit dsDNA HS Assay Kit ...
-
bioRxiv - Immunology 2024Quote: ... Total RNA samples (5-500 ng) were hybridized with NEBNext rRNA Depletion Kit v2 (Cat# E7400; New England Biolabs) to diminish rRNA from the samples ...
-
bioRxiv - Molecular Biology 2024Quote: ... Genomic DNAs (gDNAs) were extracted using the Monarch HMW DNA extraction kit for Tissue from New England Biolabs (NEB) with the following modifications ...
-
bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). sPOINT was performed in the same manner with the following difference ...
-
bioRxiv - Biochemistry 2024Quote: ... a vector containing an N-terminal Thioredoxin-His10 tag using a Gibson Assembly® Cloning Kit (New England BioLabs). Plasmids were extracted and transformed into competent E ...
-
bioRxiv - Molecular Biology 2024Quote: ... Colonies were selected and grown overnight in LB to extract plasmid DNA using a MiniPrep kit (NEB, Massachusets, USA) using manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... and PCR enrichment were performed sequentially using the NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB). Finally ...
-
bioRxiv - Neuroscience 2024Quote: ... and multiplexed RNA-seq libraries were generated using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB). Libraries were sequenced (75 bp ...
-
bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... 100ng input RNA was used for library preparation with the NEBNext Globin & rRNA depletion kit (New England Biolabs, E7750X) according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... The cDNA for catalytically inactive E447D mutant was generated through mutation of StcEΔ35 using the Q5 Site-Directed Mutagenesis Kit (Cat # E0552S, New England Biolabs) with primers 5’-TCAGTCATGACGTTGGTCATAATTATG-3’ and 5’-ACTCATTCCCCAATGTGG-3’ ...
-
bioRxiv - Microbiology 2024Quote: ... DNA fragmentation was performed using the NEBNext® Ultra™ II FS DNA Library Prep Kit (New England Biolabs), an enzymatic fragmentation assay with an average fragment size of 380 bp ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA) following the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... EM-seq was performed with 100ng of purified genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) and following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Digested oligos and vector were gel-purified with Qiagen gel purification kit and ligated by T4 DNA ligase (NEB). Ligate was transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
-
Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were created according to the manufacturer’s protocol using NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
-
bioRxiv - Genetics 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) without size selection ...
-
bioRxiv - Molecular Biology 2024Quote: ... libraries were prepared for all samples using the NEB Next Ultra II Directional RNA Library Prep Kit (NEB, E7760S) according to manufacturer protocol ...
-
bioRxiv - Immunology 2024Quote: Site-directed mutants in this study were generated using a Q5 site directed mutagenesis kit according to the manufacturer’s instructions (NEB). Briefly ...
-
bioRxiv - Molecular Biology 2024Quote: 50 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol but at one-quarter volume (5 µl) ...
-
bioRxiv - Microbiology 2024Quote: ... part of the ONT Kit) to the RNA sample using a mix of 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
-
bioRxiv - Microbiology 2024Quote: ... RIG-I and ISG15 were determined using Luna Universal One-Step RT-qPCR Kit according to the manufacturer’s instructions (New England Biolabs). The primer sequences are available upon request ...
-
bioRxiv - Neuroscience 2024Quote: The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs). Briefly ...
-
bioRxiv - Molecular Biology 2024Quote: ... The purified DNA samples were then used for library preparation using NEBNext UltraII DNA Library prep kit (NEB, E7370L) and were sequenced at the iGE3 platform of the University of Geneva.
-
bioRxiv - Molecular Biology 2024Quote: ... Library preparation was carried out on the purified DNA using NEBNext Ultra II DNA Library Prep Kit (NEB #E7645) and sequenced at 100 million reads per sample on a NovaSeq6000 using 75-nucleotide read length paired-end sequencing.