Labshake search
Citations for New England Biolabs :
7001 - 7050 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Amplified fragments of ∼4.5 kb corresponding to gokushovirus prophages were purified from agarose gels using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 µl ddH2O ...
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bioRxiv - Genomics 2019Quote: ... DNA library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Ipswich, USA). We employed 15ng of adapter ligated DNA and performed amplification with 10 cycles of PCR ...
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bioRxiv - Bioengineering 2019Quote: ... Sequencing libraries were generated using a NEBNext UltraTM RNA library prep kit Illumina (New England Biolabs, Ipswich, MA, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... ChIP DNA was extracted and prepared for high throughput sequencing using a DNA library preparation kit for Illumina (NEB). ChIP-seq libraries were sequenced on a HiSeq X Ten Platform (Illumina).
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bioRxiv - Molecular Biology 2019Quote: ... The concentrations for the final products were determined using the NEBNext Library Quant Kit for Illumina (New England BioLabs) by qPCR.
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bioRxiv - Genomics 2019Quote: ... The reverse transcribed samples were subjected to PCR for 15 cycles by adding 50μL of the PCR mastermix from the NEB small RNA for Illumina library prep kit (NEB), 5μL of the Universal adapter and 5μL of a unique indexed adapter (see NEB library pooling guidelines for suitable adapter combinations for three sample sequencing runs) ...
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bioRxiv - Microbiology 2019Quote: ... cDNA libraries were prepared using “NEBNext® Ultra™ RNA Library Prep Kit for Illumina®” (NEB#E7770S®). Libraries were sequenced in collaboration with the Chan Zuckerberg Biohub in San Francisco on an Illumina MiSeq in 150 bp paired-end runs ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA of RIN (RNA integrity number) more than 7 is subject to NEBNext Small RNA library kit (NEB). After final PCR amplification with indexed-adaptors ...
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bioRxiv - Synthetic Biology 2019Quote: ... Genomic DNA templates were obtained from Muc14a fragments previously cloned in a pMiniT 2.0 vector using PCR Cloning Kit (NEB) and primers kmer Muc14_F and 58537687_F ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 9 cDNA libraries were prepared with an NEBNext Ultra II RNA Library Prep kit (NEB, cat:E7770S) and sequenced with an Illumina HiSeq4000 ...
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bioRxiv - Systems Biology 2020Quote: ... A total of 8 cDNA libraries were prepared with an NEBNext Ultra II RNA Library Prep kit (NEB, cat:E7770S) and sequenced with an Illumina HiSeq4000 ...
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bioRxiv - Neuroscience 2019Quote: ... RNA libraries were prepared using an established protocol with NEBNExt Ultra II Directional Library Prep Kit (NEB, Lynn, MA). Each library was made with one of the TruSeq barcode index sequences ...
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bioRxiv - Genetics 2019Quote: ... Libraries from 50 ng of genomic DNA were constructed using NEBNext Ultra II FS DNA Library Prep Kit (NEB), following manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... Targeting constructs with appropriate homology arms were generated by Gibson assembly using the Gibson Assembly Master Mix kit (NEB). Targeting constructs were designed such that Cas9 recognition sites were disrupted by the presence of the LoxP sites ...
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bioRxiv - Pathology 2020Quote: ... sequencing libraries were constructed with NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and libraries were sequenced using Illumina Hiseq 4000 platform (Illumina) ...
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bioRxiv - Immunology 2019Quote: ... Sequencing libraries were generated from sonicated DNA with the NEBNext Ultra II DNA Library Prep kit (New England BioLabs). Ligation products were purified with SPRIselect beads (Beckman Coulter ...
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bioRxiv - Epidemiology 2020Quote: ... cDNA was produced from viral RNA using random hexamers and the Protoscript II First Strand cDNA synthesis kit (NEB). The genome was amplified using a multiplex PCR scheme designed to produce overlapping 500bp amplicons across the whole coding region of the recent South American genotype I outbreak clade (8,18) ...
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bioRxiv - Molecular Biology 2020Quote: ... on an Agilent mx3005p instrument using New England Biolabs NEBNext® Library Quant Kit for Illumina (NEB, cat#E7630S). For each amplicon library ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... Leishmania DNA (0.0017 ng/μL) was then enriched from the human DNA (25ng/μL) using NEBNext Microbiome DNA Enrichment Kit (NEB) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2019Quote: All plasmids were linearized with NotI and transcribed in vitro using the HiScribe(tm) T7 ARCA mRNA kit (NEB). mRNA was purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... and library preparation was conducted using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) according to manufacturer’s protocols ...
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bioRxiv - Biochemistry 2019Quote: peYFP-C1-PALB2 was modified to be resistant to PALB2 siRNA by Q5 Site-Directed Mutagenesis Kit (NEB, E0554) using primers JYM3892/3893 (Table S4) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10x diluted T7 RNA Polymerase mix (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs, USA). After linear amplification ...
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bioRxiv - Microbiology 2021Quote: ... and RNA-seq libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) as per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... and 10x diluted T7 RNA Polymerase mix (HiScribe™ T7 High Yield RNA Synthesis Kit, New England Biolabs, USA). After linear amplification ...
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bioRxiv - Plant Biology 2020Quote: ... 34 µL DNase-free water was added to 16 µL precipitated DNA and adaptors were ligated to DNA fragments according to the manufacturer’s instructions (NEBNext Multiplex Oligos for Illumina kit NEB). After ligation ...
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bioRxiv - Pathology 2021Quote: ... Fragmented PCR products were repaired and dA-tailed using the NEBNext End Repair/dA Tailing kit (New England Biolabs) according to the manufacturer’s instructions and subsequently barcoded with Illumina-compatible ...
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bioRxiv - Molecular Biology 2021Quote: ... Adaptor ligation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, New England Biolabs), and library pre-amplification was performed by 9 cycles of PCR ...
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bioRxiv - Genomics 2019Quote: ... We then cloned all three libraries into the pGL4.23c plasmid using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 2017) using primers designed by NEBaseChanger and the Q5 Site-Directed Mutagenesis Kit (New England Biolabs. Cat. No.: E0554S). The mutations were confirmed by DNA sequencing.
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR was conducted in technical triplicate using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s instructions in 10 µl reaction volumes and reactions were run on a CFX Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... THUNDERBIRD Probe qPCR Master Mix (Toyobo) for MERS-CoV and Luna Universal Probe One-Step RT-qPCR Kit (NEB) for SARS-CoV-2.
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bioRxiv - Microbiology 2020Quote: ... The Cas9/gRNA expression construct pTREX-n-Cas9 was first modified using the Q5 mutagenesis kit (NEB, Ipswich, Massachusetts), primers 5’-CCCAAAAAGAAAAGGAAGGTTGATTAGAAGCTTATCGATACCGTCGAC-3’ and 5’-GTCCTCGACTTTTCGCTTCTTTTTCGGGTCGCCTCCCAGCTGAGA-3’ ...
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bioRxiv - Genetics 2020Quote: ... DNA that migrated between the 400 and 600 bp was extracted using Monarch DNA Gel Extraction Kit (NEB T1020L). The pooled library DNA concentration was determined using Qubit dsDNA BR Assay Kit (ThermoFisher Scientist Q32853) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Each library was barcoded and sequenced in one lane of an Illumina HiSeq 2500 ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA for RNA and PARE sequencing was obtained by purification with the NEB mRNA isolation kit (New England Biolabs). RNA for sRNA library preparation was enriched with the mirVana miRNA Isolation Kit (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... Strand-specific RNA-seq library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, Ipswich, MA) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2019Quote: Illumina libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit (New England Biolabs, Frankfurt, Germany) following the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: sgRNAs were generated by in vitro transcription using the Hiscribe T7 high yield RNA synthesis kit (New England Biolabs). Purified sgRNA (0.5 μg ...
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bioRxiv - Molecular Biology 2021Quote: ... The RNA-seq libraries were constructed by NEBNext Ultra II RNA Library Prep kit (NEB, Cat: E7775, E7335, E7500) and sequenced by Illumina NextSeq 500 system (Duan et al. ...
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bioRxiv - Genomics 2021Quote: ... Libraries were constructed using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs, Frankfurt am Main, Germany) following the manufacturer’s description ...
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... ERK7-mAID-HA (TGGT1_233010) was obtained by transfecting a gRNA obtained by Q5 site-directed mutagenesis kit (New England Biolabs) on the pSAG1::Cas9-U6::sgUPRT vector (Shen et al ...
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bioRxiv - Immunology 2020Quote: ... Sequencing libraries were generated from sonicated DNA with the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). Ligation products were purified with SPRIselect beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... R972A mutations were introduced into the aforementioned IRS2 clones using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) with the primers 5’ - AGA TTA TAT GAA TAA GTC CAC TGT CAG ATT ATA TG - 3’ and 5’ - GAC AGT GGA CTT GCC TGG CGA GAG TCT GAA CT - 3’ according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: 5’-Phosphorylated linker (Oligonucleotide K, Supplementary Table 6) was adenylated using a 5’ DNA Adenylation Kit (New England Biolabs) at 20x scale and purified by TRIzol extraction as previously described56 ...
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bioRxiv - Microbiology 2019Quote: ... The pJ107106 promoter was converted to pJ107111 (Zucca et al., 2015) using the Q5 site-directed mutagenesis kit (NEB), with suggested protocols ...
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bioRxiv - Genomics 2021Quote: ... High-molecular weight DNA was extracted from blood with a Bionano Plant Tissue DNA isolation kit (Bionano Genomics) and digested with Nt.BspQI nickase (New England Biolabs). After labeling and staining ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760, New England Biolabs, Inc.). Paired-end sequencing (75 bp ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® ultra™ II directional RNA library prep kit for Illumina (NEB #E7760, New England Biolabs, Inc.). Paired-end sequencing (75 bp ...
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bioRxiv - Genetics 2020Quote: ... TruSeq-barcoded RNA-seq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) and each library was quantified via Qubit 2.0 (dsDNA HS kit ...