Labshake search
Citations for New England Biolabs :
7351 - 7400 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Eluted DNA fragments were amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ChIP sequencing libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit reagents (NEB, Ipswitch, MA) according to the procedure recommended by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... which was subsequently cloned into linearized pUHE-21 (106) using NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Construct was verified by Sanger DNA sequencing with primers 146 and 156.
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bioRxiv - Microbiology 2023Quote: The pGEX-6P-PrkA1-338 plasmid (Table 2) was constructed by a two-part ligation (NEB Quick Ligation kit) of BamHI- and NotI-linearized pGEX-6P and PCR-generated prkA1-338 amplified with primers AS21 and AS81 (Table 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Immunology 2023Quote: ... RNA sequencing libraries were produced by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L). Quantification of the library was performed using a dsDNA HS Assay Kit and Qubit (Molecular Probes ...
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bioRxiv - Molecular Biology 2023Quote: ... The sequencing library was generated using a NEBNext Ultra DNA Library Prep Kit for Illumina (New England BioLabs, USA). The concentration and quality of the library was analysed with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... low range ssRNA ladder (N0364S) and the Monarch RNA Cleanup Kit (T2030L) were purchased from NEB (Ipswich, MA, USA). T4 DNA ligase (EL0013) ...
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bioRxiv - Plant Biology 2023Quote: ... the cDNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). The resulting libraries were measured using the Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... mRNA was reverse transcribed into cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (BioLabs, Ipswich, MA, USA). Ribosomal Protein L32 (RPL32 ...
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bioRxiv - Cell Biology 2023Quote: ... Envelope gene mutations were introduced into WT cDNA sequence using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). All cloned were sequenced (Eurofins).
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bioRxiv - Genomics 2023Quote: ... libraries were constructed with the NEBNext Small RNA Library Preparation Kit according to manufacturer’s recommendations (NEB, cat. no. E7330). See Supplemental File 6 for detailed protocol.
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bioRxiv - Cancer Biology 2023Quote: Kinase-dead mutant (K37R) of Nek2A was derived from Nek2A in pJP1563 using Q5 Site-Directed Mutagenesis Kit (NEB) following the instructions of manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated from 17 hpf Ciona robusta larvae by Monarch Total RNA miniprep kit (New England BioLabs Inc.). cDNA was produced by Omniscript reverse transcription kit (QIAGEN) ...
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bioRxiv - Cell Biology 2023Quote: ... Library preparation followed the NEBNext Ultra II DNA Library Prep Kit for Illumina protocol (New England Biolabs, MA, US). Size selection of adapter-ligated DNA was performed as described in the protocol ...
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bioRxiv - Microbiology 2023Quote: ... The complementation plasmid was constructed by Gibson assembly using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). In brief ...
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bioRxiv - Plant Biology 2023Quote: ... the SQK-RNA002 kit (Oxford Nanopore Technologies) was used together with NEBNext® Quick Ligation Reaction Buffer (NEB B6058), T4 DNA Ligase 2M U/ml (NEB M0202) ...
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bioRxiv - Microbiology 2023Quote: ... and DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, NEB). DNA purifications and size selections were made using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... and DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, NEB). DNA purifications and size selections were made using Ampure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... 200ng of cleaned DNA was used as an input for conversion using a Enzymatic Methyl-seq Kit (NEB E7120S) according to the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2023Quote: Whole genome DNA methylation sequencing was performed using the NEBNext Enzymatic Methyl-seq (EM-seq) Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Non-β-lactamase genes used as controls were cloned into the pBAD18 using the Gibson Assembly Cloning Kit (NEB). pBAD (RBS)_fwd/rev primer pairs were used to amplify the plasmid backbone ...
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bioRxiv - Microbiology 2023Quote: cDNA was generated starting from 150 ng of RNA using ProtoScript® II First Strand cDNA Synthesis Kit (NEB) using random hexamers in agreement with the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNA libraries were prepared using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2023Quote: ... In vitro transcription of sgRNAs was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB). Then ...
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bioRxiv - Microbiology 2023Quote: ... and RNA-Seq libraries were generated using the NEBNext® Ultra II Directional RNA Library Prep Kit (NEB # E7760L). Two biological replicates were sequenced using the Illumina NextSeq 550 platform to generate 150 bp PE reads.
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA libraries were prepared using NEBNext® UltraII RNA library preparation kit (NEB, New England Biolabs, MA, USA) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and cDNA libraries were prepared using NEBNext® UltraII RNA library preparation kit (NEB, New England Biolabs, MA, USA) using the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared using 50ng RNA according to the manufacturer’s recommendation for the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the Illumina NextSeq 500.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR product was cleaned and concentrated with the Zymo Research kit following the manufacturer’s protocol and then digested by DpnI (New England BioLabs) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... The pNH605-RIM11 was amplified by making use of the Q5® Site-Directed Mutagenesis Kit (New England Biolabs) following the instructions of the manufacturer to generate pNH605-RIM11 Y199F ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA fragments were then constructed into libraries using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). 100M reads/read pairs per library were requested ...
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bioRxiv - Plant Biology 2023Quote: ... ChIP DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... This was followed by two sets of enzymatic conversion steps using the Enzymatic Methyl-seq kit (NEB, Cat# E7120L) to differentiate cytosines from 5mC and 5hmC ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB, #E7760) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Libraries in this paper were prepared using NEB’s rRNA Depletion Kit (Bacteria) for rRNA removal with beads (NEB #E7860). Post rRNA removal samples were prepared for either Illumina or Singular Sequencing using NEBNext Ultra II RNA Library Prep Kit for Illumina with Beads (NEB#E7775) ...
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bioRxiv - Genomics 2023Quote: ... Linearized destination vectors were gel purified using 0.7% agarose gels and extracted with the Monarch DNA Gel Extraction Kit (New England Biolabs), before further purification by isopropanol precipitation.
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bioRxiv - Genomics 2023Quote: Sample DNA concentrations were quantified using Qubit and libraries prepared using NEBNext Ultra II DNA library preparation kit (NEB) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Genomics 2023Quote: ... Total (“steady-state”) RNA was converted to sequencing libraries using NEB polyA capture kit (product no) followed by NEB Ultra directional II RNA-seq library kits ...
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bioRxiv - Genomics 2023Quote: ... or 100 cGy) was processed for bisulfite sequencing library construction using NEBNext Ultra II DNA Library Prep Kit (NEB) along with methylated and indexed NEXTflex Bisulfite-seq Barcodes (Bio scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... An RNA-seq library was prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra™ DNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... RNA sequencing library preparation was performed utilizing the NEBNext Ultra RNA Library Prep Kit for Illumina by following the manufacturer’s recommendations (NEB). Sequencing libraries were validated on the Agilent 2100 Bioanalyzer System (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... NEBNext® UltraTM II Non-Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., MA, USA) was used for the remaining steps of library construction ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, UK) following manufactures’ protocol ...
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bioRxiv - Microbiology 2023Quote: ... Removal of rRNA from the samples was carried out using NEBNext Ultra directional RNA library prep kit (NEB, MA). Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... a KOZAK sequence was added in front of the TY1 region with the Q5 Site-Directed Mutagenesis Kit (NEB) using primers F167 and F168 ...