Labshake search
Citations for New England Biolabs :
7101 - 7150 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The sequencing library was prepared with a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). We purified polyA+ transcripts with the NEBNext Poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... HQ116417) was cloned into a pOPINF vector (24) using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). The protein ...
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bioRxiv - Molecular Biology 2021Quote: ... NEBNext Ultra II DNA Library Prep Kit for Illumina was used to make the DNA libraries (NEB, #E7645S/L), which were sequenced on the Illumina NovaSeq (PE-150 ...
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bioRxiv - Genetics 2020Quote: ... Bisulfite conversion and library construction were carried out as before using the EpiMark Bisulfite Conversion Kit (NEB cat# E3318) according to manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2021Quote: PCR products were subject to in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB), which relies on the T7 RNA polymerase to initiate transcription from a T7 promoter sequence (present in our fragments) ...
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bioRxiv - Bioengineering 2020Quote: ... followed by library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). Sequencing was performed using a NextSeq 500 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward: 5’-TCCGGCACAAACGGCACA-3’, reverse: 5’-GATGGCGTGGAACCATGTC-3’) via Q5 SiteDirected Mutagenesis Kit (NEB). All plasmids were confirmed by gene sequencing (BGI Beijing) ...
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bioRxiv - Microbiology 2022Quote: ... which was synthesized using the HiScribe T7 High Yield RNA Synthesis Kit according to manufacturer’s guidelines (New England Biolabs). Next ...
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bioRxiv - Microbiology 2022Quote: ... RNA passing quality control was converted to a sequencing ready library using the NEBNext Ultra II Directional RNA library kit with polyA selection as per the manufacturer’s instructions (New England Biolabs). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems ...
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bioRxiv - Immunology 2022Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries for RNA-seq were made with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... then processed for RNA-seq library prep with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, E7530L), and finally sequenced on an Illumina NextSeq 500 ...
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bioRxiv - Cell Biology 2022Quote: ... The RNA-Seq library was prepared by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center) ...
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bioRxiv - Neuroscience 2022Quote: ... and RNA was isolated according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit; #T2010S New England Biolabs). As a quality control ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA fragments were converted to library using NEBNext® Small RNA Library kit (ref#E7330S, New England Biolabs, UK) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... prior to library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) with poly(A ...
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bioRxiv - Molecular Biology 2022Quote: ... ChIP samples were analyzed by qPCR or subjected to library preparation according to the manufacturer’s protocol (NEBNext Ultra II DNA Library Prep Kit for Illumina, NEB) using Dual Index Primers (NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Bioengineering 2022Quote: ... the 5S and Saba PCR products were purified using Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB, # T1030). The purified DNA sequence was then analyzed through Sanger sequencing (GENEWIZ from Azenta ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro transcription was performed using HiScribe™ T7 or SP6 High Yield RNA Synthesis Kit (New England Biolabs). RNA was subsequently purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Cancer Biology 2022Quote: ... mRNA was isolated from 1.5 μg of total RNA using the kit NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). NGS libraries were prepared from the purified mRNA using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... The product bend was cut and DNA was extracted from the gel by Monarch DNA Gel extraction Kit (NEB). 32P-GTP labelled U1 RNA was prepared by in vitro transcription as described previously with addition of 0.5 μL of α-32P-GTP (3.3 μM ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were directly subjected to qRT-PCR using Luna Universal One-Step RT-qPCR Kit (NEB, #E3005L). The primer sets used in qRT-PCR to detect CI-MPR and CD-MPR mRNA are 5’-CATTCAGTGGGTGACTCTGTT-3’/5’-TGCTCTGGACTCTGTGATTTG-3’ and 5’-GCTCTAGTGAAGAGGCTGAAAC-3’/ 5’-GCACACCCTGAAGATGTAGATG-3’ ...
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bioRxiv - Biophysics 2022Quote: ... The 8C-8A(IL125) chimera cDNA construct was generated using the Phusion High-Fidelity PCR kit (New England BioLabs). All cDNAs were tagged on their carboxy terminus with Myc-DDK epitopes ...
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bioRxiv - Developmental Biology 2022Quote: ... Library preparation was performed with the NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB, Cat #E7645S) using 25 μL of CUT&RUN DNA as input ...
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bioRxiv - Cell Biology 2022Quote: ... The amplicons (200 fmol) were first end-repaired with the NEBNext Ultra II End repair kit (New England Biolabs), then purified using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... ICL3 and S305A mutations were generated using back-to-back primers on pCDNA3.1-HCAR1-Flag vector by Q5 Site-Directed Mutagenesis Kit (NEB). Fluorogen activating peptide fusion to HCAR1 was synthesized with insertion of HCAR1 using BsmI site into pMFAP-β1 vector (Spectragenetics) ...
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bioRxiv - Cell Biology 2022Quote: ... Purified DNA was cloned into pAV0714 (Vještica et al., 2020) using NEBuilder® HiFi DNA assembly kit (NEB #E2621). After being verified by Sanger sequencing ...
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bioRxiv - Genomics 2022Quote: ... ChIP samples were processed for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instruction and sequenced on a Next-Seq 500 Sequencer (Illumina ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using NEB Next® Ultra™ RNA Library Prep Kit (NEB, San Diego, CA, USA), employing AMPure XP Beads to purify the products of the reactions during the library prep ...
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bioRxiv - Genetics 2022Quote: Libraries were generated with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). mRNA was enriched from total RNA (250 ng ...
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bioRxiv - Genetics 2022Quote: All of the plasmids created by Gibson assembly used the NEBuilder HiFi DNA Assembly kit (New England Biolabs, #E2621). To construct the URA3 RRM3 plasmid ...
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bioRxiv - Genomics 2022Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit 2.0 (dsDNA HS kit ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The purified UVRh1 amplicons were cloned into the minT vector using the PCR cloning kit and following the manufacturer’s protocol (NEB). The cloned amplicons were sequenced by Retrogen Inc ...
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bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was purified from single-humanized Hsα1 and quintuple-humanized Hsα1,α2,α3,α4,α7 strains using the Monarch Nucleic acid purification kit according to the manufacturer’s protocol (NEB). Spheroplasts were obtained before the genomic DNA extraction for a high-quality DNA prep ...
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bioRxiv - Plant Biology 2022Quote: ... RNA samples were used to generate sequence libraries using a library prep kit from New England Biolabs (NEB #E7530). Paired-end sequence reads of 75 bp were generated at the TUFTs genomic center at the Tufts University School of Medicine using the Hi-Seq Illumina platform ...
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bioRxiv - Microbiology 2022Quote: ... the start codon of each uORF was mutated using a Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The sequence additions and mutations of all plasmids were confirmed by DNA sequencing (Source Bioscience).
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were constructed using NEB Next® UltraTM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Plant Biology 2022Quote: ... RNA Libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced as single end reads using the NextSeq500 platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to the NEBNext Ultra RNA II directional Library Prep kit (New England Biolabs, MA, USA) after NEBNext rRNA depletion (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... Library preparation was performed with the NEBNext Ultra DNA Library prep kit according to the manufacturer’s instructions (NEB, E7370L). 4C-seq libraries were sequenced on an Illumina Hiseq2500 platform (50 cycles ...
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bioRxiv - Microbiology 2022Quote: ... xanthus cells grown on 1.5% agar supplemented with 1% CTwas extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB)) ...
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bioRxiv - Immunology 2022Quote: ... Library quality and quantity were assessed by qPCR method using NEB Next Library Quant Kit for Illumina (NEB, # E7630) and Qubit ...
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA of the gram-negative bacteria was extracted using the Monarch DNA Gel Extraction Kit (New England Biolabs). After isolation of the genomic DNA ...
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bioRxiv - Microbiology 2022Quote: ... for removal of genomic DNA before it was purified using the Monarch RNA cleanup kit (New England Biolabs, USA). The RNA and potential DNA contamination concentrations were quantified using Qubit RNA BR and Qubit dsDNA HS assay kits ...
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bioRxiv - Microbiology 2022Quote: ... ShdA III and ShdA IV homologues were synthesised in vitro using the PURExpress cell-free transcription/translation kit (NEB), from PCR products ...
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bioRxiv - Microbiology 2022Quote: ... and libraries were quantitated with qPCR (Bio-rad CFX96 Touch Real-Time PCR, NEB Library Quant Kit for Illumina). Libraries were normalized to 0.5 nM and pooled ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Antisense strand DNA was digested in the samples using the Uracil-Specific Excision Reagent Enzyme Kit (New England Biolabs), followed by a PCR to amplify the cDNA ...