Labshake search
Citations for New England Biolabs :
7451 - 7500 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... a vector containing an N-terminal Thioredoxin-His10 tag using a Gibson Assembly® Cloning Kit (New England BioLabs). Plasmids were extracted and transformed into competent E ...
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bioRxiv - Genomics 2024Quote: ... Small RNA libraries were generated using the NEBnext small RNA library preparation kit according to instructions (New England BioLabs). small RNA high-throughput sequencing libraries were sequenced for 101 cycles using NovaSeq X Plus ...
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bioRxiv - Genetics 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) without size selection ...
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bioRxiv - Immunology 2024Quote: Site-directed mutants in this study were generated using a Q5 site directed mutagenesis kit according to the manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... EM-seq was performed with 100ng of purified genomic DNA using NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) and following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested oligos and vector were gel-purified with Qiagen gel purification kit and ligated by T4 DNA ligase (NEB). Ligate was transformed into Stbl3 competent bacteria (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... All connections between genes and linearized vectors were achieved by Gibson assembly (Gibson assembly kit, New England BioLabs, UK). The shell-(csoS2-NM) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Lettuce variant sequences were cloned into retron Eco2 plasmid using Q5® Site-Directed Mutagenesis Kit (New England Biolabs). Primer sequences and annealing temperatures were generated using the NEBaseChanger™ tool.
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bioRxiv - Synthetic Biology 2024Quote: ... RNA strands were transcribed in-house and column-purified using the Monarch® RNA Cleanup Kit (New England Biolabs). The concentration of oligonucleotides was quantified by UV-vis spectrophotometry using a Thermo Fisher Scientific NanoDrop 2000 Spectrophotometer ...
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bioRxiv - Synthetic Biology 2024Quote: ... and the amplified plasmid DNA was extracted using the Monarch® DNA Gel Extraction Kit (New England Biolabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: All transcription templates were generated using PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs, catalog no. E0553) of an oligo that includes a T7 RNAP promoter ...
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bioRxiv - Molecular Biology 2024Quote: ... All qPCRs were performed using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) and a Step One Plus qPCR machine (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from bulk liver tissue using the Monarch total RNA miniprep kit (New England Biolabs, Ipswich, MA) after homogenization with steel beads using a TissueLyser LT bead mill (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: 50 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol but at one-quarter volume (5 µl) ...
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bioRxiv - Microbiology 2024Quote: ... part of the ONT Kit) to the RNA sample using a mix of 3μl NEBNext Quick Ligation Reaction Buffer (New England BioLabs), 0.5μl RNA CS (ONT Kit) ...
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Fluoroquinolone-Specific Resistance Trajectories in E. coli and their Dependence on the SOS-ResponsebioRxiv - Microbiology 2024Quote: ... Genomic DNA libraries were created according to the manufacturer’s protocol using NEBNext Ultra II FS DNA Library Prep kit for Illumina (New England BioLabs) in combination with NEBNext multiplex oligos for Illumina (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Neuroscience 2024Quote: The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer’s instructions (New England Biolabs). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ChIP-seq libraries were built using an NEBNext® Ultra™ II DNA Library Prep Kit (NEB #E7645S/L) with immunoprecipitated or input DNA ...
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bioRxiv - Microbiology 2024Quote: ... RIG-I and ISG15 were determined using Luna Universal One-Step RT-qPCR Kit according to the manufacturer’s instructions (New England Biolabs). The primer sequences are available upon request ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was utilized to construct the sequencing libraries using NEBNext Ultra RNA Library Prep Kit for Illumina as per the manufacturer’s protocol (NEB) and paired-end sequencing was performed with NovaSeq 6000 platform (Illumina).
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Monarch HMW DNA Extraction Kit for Tissues (New England Biolabs, Ipswitch, MA, USA). Long-read sequencing was performed on the GridION platform (Oxford Nanopore Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... To ensure this we performed the Oligonucleotide Clean-up protocol of the Monarch PCR & DNA Clean-up kit (NEB) on 10 PCR reaction tubes ...
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bioRxiv - Microbiology 2024Quote: ... cloned into the pre-assembled vector using the NEBridge® Golden Gate Assembly Kit (BsaI-HF® v2; NEB). A synonymous point mutation (A17973G ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng of DNA template was transcribed using a HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s protocol for 11 hr ...
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bioRxiv - Microbiology 2024Quote: ... NGS libraries were prepared by NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB, MA, USA) following manufacturer instructions and sequencing was conducted by NovaSeq 6000 (PE150 ...
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bioRxiv - Synthetic Biology 2024Quote: All plasmid E.coli plasmid DNA minipreps were performed using the NEB Monarch Plasmid Miniprep Kit and Protocol (NEB #T1010). Prior to starting the miniprep protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... All reactions were then digested with 1 µl ExoI and purified using the Monarch DNA Gel Extraction Kit (NEB), eluting in 10 µl of elution buffer (10 mM Tris•Cl pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA libraries were then constructed from 50 ng of depleted RNA using the NEBNext Ultra II Directional RNA library prep kit following manufacturer instructions (NEB). Libraries were size-selected to 200-700 bp using SPRIselect beads and quality was assessed on the Agilent Bioanalyzer using High Sensitivity DNA chips ...
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bioRxiv - Microbiology 2020Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ DNA Library Prep Kit (New England BioLabs Inc., Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The resulting fragment was inserted into the HindIII-BamHI sites of pJB866 by an NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The nucleotide sequence of the insert was confirmed by sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... mRNA Magnetic Isolation Module and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs). Library quality was checked on an Agilent TapeStation 4150 and quantified by Qubit 2000 fluorometer (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... mRNA Magnetic Isolation Module and libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs). Library quality was checked on an Agilent TapeStation 4150 and quantified by Qubit 2000 fluorometer (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... Indel frequencies at the SaCas9 target site were assessed via a T7E1 assay with the EnGen Mutation Detection Kit (NEB), using the manufacturer’s recommendations ...
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bioRxiv - Biophysics 2021Quote: ... The AcrIIA4 expression vector was modified to express AcrIIA11 with a C-terminal NLS and HA tags using the HiFi Assembly Kit (NEB).
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bioRxiv - Neuroscience 2021Quote: ... were ligated to end-repaired and A-tailed chromatin using components from NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) (25).
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bioRxiv - Cancer Biology 2021Quote: ... RNA was subsequently sent for either library preparation and RNA-sequencing to Genewiz or cDNA preparation via a two-step process beginning with Lunascript RT Supermix Kit (#E3010, New England Biolabs). qRT-PCR was performed on cDNA from samples for genes of interest using the Luna Universal qPCR Master Mix (#M3003 ...
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bioRxiv - Cancer Biology 2021Quote: ... phosphorylated and bead-purified in preparation for A-tailing using a custom NEB Paired-End Sample Prep Premix Kit (New England Biolabs). Illumina sequencing adapters were ligated for 15 minutes at 20°C and adapter-ligated products were then bead-purified and enriched with 10 cycles of PCR using primers containing a hexamer index that enables library pooling.
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bioRxiv - Cancer Biology 2021Quote: ... The PCR products were resolved by agarose gel electrophoresis and purified using the Monarch Gel Extraction kit (New England Biolabs). Purified PCR products were Sanger sequenced by Genewiz using the BPNT1_F_Seq primer ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated as described above and sample preparation was performed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420S/L; NEB) according to the protocol ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... RNA-Seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs # E7420S) by following the company's protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were constructed at the Center for Health Genomics and Informatics using the NEBNext Ultra II Directional RNA Library prep kit (New England Biolabs) with ribosomal RNA depletion ...
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bioRxiv - Cell Biology 2020Quote: ... Full-length 265 nt 5’ UTR of SARS-CoV-2 was subcloned to replace the 5’ UTR of human CMV in the pLV-mScarlet vector using a Hifi one-step kit (Gibson Assembly, NEB). Full-length ...
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bioRxiv - Genomics 2020Quote: ... The DNA was sheared to 650 bp using Covaris S220 and subsequently used for DNA-seq library preparation with NEBNext Ultra DNA Library Prep Kit (New England Biolabs). The paired-end sequencing datasets with 101 nt read length (PE101 ...
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bioRxiv - Cell Biology 2019Quote: ... One μg of purified RNA was reverse transcribed into cDNA using the Protoscript II Reverse Transcriptase kit (New England Biolabs). Quantitative real time polymerase chain reactions (qRT-PCR ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR fragments were cloned into the linearized pUC19 vector using the Gibson Assembly Cloning Kit (E5510S; New England Biolabs).
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bioRxiv - Cell Biology 2020Quote: Site-directed mutagenesis of NES and hydrophobic-stretch coding sequences was performed using the Q5 site-directed mutagenesis kit (New England Biolabs) with either pCPLJJ50 ...
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bioRxiv - Cell Biology 2020Quote: ... the rRNA-depleted RNA was used to build the RNA-seq library with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... 100 ng of total RNA from each sample was subjected to rRNA depletion with NEBNext rRNA Depletion Kit (New England Biolabs) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... The remaining steps of library preparation were done using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs ...
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bioRxiv - Cell Biology 2019Quote: ... and subsequently directional RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420, New England Biolabs). 75 bases paired end sequencing was performed on the Illumina HiSeq 2500 system ...