Labshake search
Citations for New England Biolabs :
7301 - 7350 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... Library preparation was carried out using the NEBNext® Ultra Directional RNA Library Prep Kit for Illumina (#E7420L, NEB). Barcoded libraries from each experimental sample were combined in equimolar concentrations of 1.5 pM prior to sequencing at 75bp x 1 (single-end ...
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bioRxiv - Microbiology 2023Quote: NEB Ultra II directional RNA-Seq Library Prep kit protocol was used to prepare libraries for mRNA sequencing (NEB). An initial concentration of 500ng of total RNA was taken for the assay ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina following the manufacturer’s instructions (NEB). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting amplicons from the second PCR were column purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs; NEB) to remove genomic DNA and first round PCR product ...
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bioRxiv - Biochemistry 2023Quote: ... Single or multiple amino acid mutations in full-length GPR161 were generated using Q5 site-directed mutagenesis kit (NEB).
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Developmental Biology 2023Quote: ... mRNA Magnetic Isolation Module and the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina according to the manufacturer’s instructions (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Developmental Biology 2023Quote: ... The library was prepared as per the user’s manual using the NEBNext Ultra II Directional RNA library Prep Kit for Illumina (E7760S, NEB). Library indexing was done with NEBNex multiplex oligos for Illumina Set 1 (E7335S ...
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bioRxiv - Cell Biology 2023Quote: ... and libraries for massive parallel sequencing were constructed with NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB). Library quality was assessed with Agilent Bioanalyzer 2100 system ...
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bioRxiv - Immunology 2023Quote: ... and the RT-qPCR master mix prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated with the NEBNext® Ultra II Directional Library Prep Kit for Illumina (New England BioLabs), modified to use oligonucleotide sequences appropriate for the sequencing pipelines of the Wellcome Sanger Institute ...
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bioRxiv - Developmental Biology 2023Quote: ... was generated from full-length CSN-5 using a Q5 site-directed mutagenesis kit per manufacturer’s instructions (New England BioLabs). Truncated CSN-5 constructs were made by PCR from full-length CSN-5 and inserted into pGEX-KG plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel purified DNA fragments were cloned into the pMiniT 2.0 vector using the NEB PCR Cloning Kit (New England BioLabs) and then transformed into NEB 10-beta competent cells (New England BioLabs) ...
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bioRxiv - Microbiology 2023Quote: ... The pools were cloned into SmaI-linearized AWP-029 with the NEBuilder® HiFi DNA Assembly kit (NEB, E2621) in two separate reactions as described above for the generation of single-gRNA mutants.
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bioRxiv - Microbiology 2023Quote: ... nLuc plasmids were linearized using SpeI and subjected to in-vitro transcription using HiScribe T7 kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England Biolabs Inc.) and index codes were added to attribute sequences to each sample ...
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bioRxiv - Evolutionary Biology 2023Quote: ... beads were directly used for off-bead PCR amplification using the Phusion High-Fidelity PCR kit (New England Biolabs) for 30 cycles with primers from library amplification (Rd1 ...
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bioRxiv - Plant Biology 2023Quote: ... the SQK-RNA002 kit (Oxford Nanopore Technologies) was used together with NEBNext® Quick Ligation Reaction Buffer (NEB B6058), T4 DNA Ligase 2M U/ml (NEB M0202) ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR amplified product was in-vitro transcribed using the HiScribe T7 High Yield RNA synthesis kit (NEB #E2040S) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... a DNA adapter (TableS6) was ligated to 3′ ends of nascent RNA using the T4 RNA ligase kit (NEB) by mixing 50 pmol adapter with 300-600 ng nascent RNA ...
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bioRxiv - Genetics 2022Quote: ... Variants were generated in the pDONR vector via site-directed mutagenesis utilizing NEBaseChanger with the Q5 mutagenesis kit (NEB) and verified by sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA samples (500ng) were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit (NEB #E6420) by following manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs #E7645L) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Sequencing libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina according to the manufacturer’s protocol (NEB) and paired-end sequencing was performed with NovaSeq 6000 platform (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... qPCR was performed to amplify libraries using the NEBUltra Ultra II DNA library prep kit for Illumina (NEB, E7645L). The final libraries were purified using AMP Pure XP beads (Beckmann Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina® (NEB England BioLabs). RNA-seq paired-end reads were sequenced on the Illumina NovaSeq 6000 platform.
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bioRxiv - Cell Biology 2022Quote: ... RNA sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina® (NEB England BioLabs). RNA-seq paired-end reads were sequenced on the Illumina NovaSeq 6000 platform.
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bioRxiv - Cell Biology 2023Quote: ... T690A mutation of RAD50 and S1336A mutation of BRCA1 were introduced using the Q5 Site-directed mutagenesis kit (NEB). The MAP7 truncation mutations were created by PCR cloning to Gateway p221 pDONR221 (Invitrogen) ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645S) with the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... were further assembled and sequenced at GIGA-Liège University (Belgium) using the NEBNext Ultra II DNA library prep kit (New England BioLabs) and then sequenced on the Illumina NextSeq 500 platform with 2 × 150 nt reads with a target of 10 million sequences per pool of ten libraries (i.e ...
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bioRxiv - Genomics 2023Quote: ... Purified DNA was submitted to library preparation using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E765). The samples were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genetics 2023Quote: ... The sgRNA was synthesized using the NEB HiScribe T7 Fast and Efficient RNA Synthesis Kit (NEB E2050S, MA, USA). After mixing 5μM~10μM sgRNAs of bmp6a and bmp6b and 5μM Cas9 protein (M0646 ...
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bioRxiv - Genetics 2022Quote: ... exon duplication and exon deletion mutants were generated by site-directed mutagenesis PCR following the manufacturer’s instructions of the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). To generate the plasmids for in vitro transcription/translation of the fusion proteins SmMADS3-GFP and CRM3-GFP ...
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bioRxiv - Immunology 2023Quote: pals-17::gfp and pals-20::wrmScarlet translational reporter constructs were assembled using NEBuilder HiFi kit (New England Biolabs) by fusing PCR products and gene fragments ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: DNA for WGS was prepared by PCR-free library preparation according to the NEBNext DNA Ultra II kit (NEB). Sequencing was performed by Illumina NextSeq 2000 P3 150PE.
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bioRxiv - Immunology 2023Quote: 1 μg of total RNA was subjected to rRNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... of ATG13 and ATG13.11-198 referred to as ATG13.1HORMA were introduced into linearised pBMN-HA-C1 using Gibson Cloning kit (New England Biolabs) to create pBMN-HA-ATG13 and ATG13.1HORMA (Addgene #186223 and #18622 respectively) ...
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bioRxiv - Molecular Biology 2023Quote: DNA templates were transcribed and translated in vitro using a custom PURExpress ΔRF-123 ΔRibosome Kit (New England Biolabs) and a final concentration of 2.2 μM ribosomes ...
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bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using NEBNext Ultra II RNA library prep kit using the manufacturer’s instructions (NEB, Ipswich, MA). Sequencing libraries were validated on the Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as via quantitative PCR (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 pmol of TP_MAAAPQKCAAA* mRNA (see “Toeprinting assays” section) and components of the PURExpress ΔRibosomes Kit (New England Biolabs). The reaction was incubated at 37ºC for 20 min and then diluted in 50 mM Hepes KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Plant Biology 2023Quote: ... Poly-A enriched libraries were prepared using the NEBNext UltraTM RNA Library Prep Kit (New England Biolabs, Ipswich, USA) for Illumina ...
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bioRxiv - Physiology 2023Quote: ... cDNA was generated from 500ng of RNA using the LunaScript RT Supermix kit (New England Biolabs, Cat. No. E3010L) according to the manufacturer’s directions ...
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bioRxiv - Systems Biology 2023Quote: ... metatranscriptomic libraries were constructed according to the NEBNext® UltraTM RNA Library Prep Kit (Illumina, New England Biolabs, MA) and sequenced on the Illumina Hiseq 2500 platform (Illumina ...
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bioRxiv - Systems Biology 2023Quote: ... Dual-indexed sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) with 10 ng or 10 uL of input DNA (whichever was lower volume) ...
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bioRxiv - Immunology 2023Quote: ... A 140-500 ng aliquot of total RNA was rRNA depleted using NEB’s Human/Mouse/Rat RNAse-H based Depletion kit (New England BioLabs). Following rRNA removal ...
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bioRxiv - Genomics 2023Quote: ... Preparing the MLI1 DNA library was performed using NEBNext Ultra II DNA Library Prep kit (New England Biolabs, USA) and sequencing on an Illumina MiSeq with paired-end 300 bp reads at the “Molecular and Cellular Biology” core facility of the IMCB SB RAS (Novosibirsk ...
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bioRxiv - Cell Biology 2023Quote: ... APOE3-C-mEm ΔSS were created from APOE3-mEm using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). The N-terminal domain constructs consist of residues 19-209 with or without residues 1-18 of the N-terminal signal peptide ...
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bioRxiv - Developmental Biology 2023Quote: ... Eluted DNA fragments were amplified using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...