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Citations for New England Biolabs :
6001 - 6050 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... PCR was performed using Q5 High-Fidelity DNA Polymerase or Q5 High-Fidelity 2X Master mix (New England Biolabs). PCR products were purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... The GFP-Rab5C PCR product and a pLXIN-I-NeoR plasmid were digested using Hpa1 (New England Biolabs – R0105) and Not1 (New England Biolabs – R3189 ...
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bioRxiv - Bioengineering 2019Quote: The pcDNA 3.1 NCadTS expression construct was generated using overlap extension PCR [5] using Phusion Polymerase (New England BioLabs). The forward and reverse primers 5’ CCA CAG TAC CCA GTC CGA TCC GCA GCC ATG GTG AGC AAG GGC GAG GAG ACC ACA ATG GGC 3’ ...
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bioRxiv - Genomics 2019Quote: ... We added 1 µL 12.5% Triton-X to each well to quench the SDS and 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB). Samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Microbiology 2019Quote: ... The wildtype and truncated Chlamydia trachomatis mreB genes were amplified by PCR with Phusion DNA polymerase (NEB, Ipswich, MA) using 100 ng C ...
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bioRxiv - Developmental Biology 2019Quote: ... library amplification was performed by setting up a PCR reaction (50 μl) with 1X Phusion HF master mix (NEB), 0.2 uM universal primer ...
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bioRxiv - Genomics 2019Quote: ... we enriched the purified DNA templates through PCR using Phusion Hot Start High-Fidelity DNA Polymerase (NEB, M0530S, USA). After purification of the PCR products (MinElute PCR Purification Kit ...
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bioRxiv - Molecular Biology 2019Quote: ... then amplified and indexed with unique library indices using NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs). PCR products were purified with AMPure XP beads (Beckman-Coulter ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µl of DNA extract was used as a template in a 20 µl PCR with Taq polymerase according to the manufacturer’s protocol (NEB). For genotyping rbm8aoz35 and foxo3bihb404 mutant embryos ...
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bioRxiv - Genetics 2021Quote: ... We added 1 µL 12.5% Triton-X to each well to quench the SDS and 12.5 µL NEBNext High-Fidelity 2× PCR Master Mix (NEB). Samples were PCR-amplified (72 °C 5 min ...
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bioRxiv - Genetics 2020Quote: ... The Rad21l1 PCR product was then cloned into pET28b using NEBuilder HiFi DNA Assembly Master Mix (NEB, Catalog #: E5520S). BL21 (DE3 ...
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bioRxiv - Neuroscience 2020Quote: ... unc-116::tagRFP::tom7 including unc-54 3’-UTR was PCR amplified using Phusion polymerase (NEB, Ipswich, MA, USA) from unc29p::unc-116::tagRFP::tom7 using following primers ...
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bioRxiv - Molecular Biology 2020Quote: σA (MSMEG_2758) and rbpA (MSMEG_3858) genes were amplified from genomic DNA by PCR with Phusion High-Fidelity DNA Polymerase (NEB) with primers #1155 + #1156 (σA ...
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bioRxiv - Cell Biology 2020Quote: PCR amplification of short fragments for screening purposes (<3 kbp) was conducted using OneTaq® (New England Bolabs (NEB), Ipswich ...
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bioRxiv - Biochemistry 2021Quote: ... c-Jun point mutations were generated by PCR-based mutagenesis using the site-directed mutagenesis kit (New England Biolabs). cDock ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl of this lysate was directly used for PCR reactions with 2× Taq start master mix (M0496L, NEB), and the two NASP clone screening primers (see primer table) ...
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bioRxiv - Biochemistry 2021Quote: ... cDNAs encoding each of the CBAF subunits were PCR-amplified using Phusion DNA polymerase in HiFi Phusion buffer (NEB) and subcloned into pLibMam vectors using NEBuilder HiFi (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... The oligo was PCR amplified with OneTaq® 2X Master Mix with Standard Buffer (New England BioLabs Inc., M0482L), followed by agarose gel purification with GenCatch Advanced Gel Extraction Kit (Epoch Life Science ...
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bioRxiv - Synthetic Biology 2021Quote: Sub-parts (modules, Supplemental Table 1) were amplified via high-fidelity polymerase chain reaction (PCR) (New England Biolabs #M0491S) using dsDNA templates and ssDNA primers listed in Supplemental Table 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... The transcription template for RNAI was produced by using p770 plasmid (from E. coli strain LK_2385) as template for PCR amplification using Q5 polymerase (NEB) and LK_3413 and LK_3414 primers to introduce a T7 class II promoter (Supplementary table 3) ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was cleaned twice with (0.9X ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was used to amplify the targeted allele (Quick-Load® Taq 2X Master Mix, New England Biolabs Inc), using the following primers (at 0.4μM) ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplicons were A-tailed by incubating 750 ng of DNA with 2 Units of Taq DNA Polymerase (NEB) and 0.2 mM dATP (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: The library was prepared for high-throughput sequencing in two rounds of PCR (Q5 High-Fidelity DNA Polymerase, NEB). In PCR1 ...
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bioRxiv - Genomics 2020Quote: ... of SEMA3C (ENST00000265361.7) were PCR amplified from neonatal LEC cDNA using Phusion high-fidelity DNA polymerase (New England BioLabs), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... library DNA was recovered using AMPure XP beads (1.8X concentration) and PCR amplified for 15 cycles (all enzymes from New England Biolabs). Libraries were sequenced on an Illumina NextSeq in 37×37 paired end mode ...
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bioRxiv - Genomics 2020Quote: ... The donor vector (mCTCF24-AID-donor-Neo) was constructed using PCR and Gibson Assembly Cloning kit (New England Biolabs). The insert cassette includes sequences that codes for a 5GA linker ...
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bioRxiv - Genomics 2019Quote: ... 12 cycles of PCR were performed as indicated by the NEBNext multiplex oligos for Illumina kit (New England Biolabs). PCR reactions were cleaned using Agencourt AMPure XP beads (Beckman).
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bioRxiv - Immunology 2020Quote: All cloning PCR amplifications were performed using the Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA) according to standard procedures.
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bioRxiv - Microbiology 2020Quote: ... Amplification of regions of interest was achieved through PCR using Q5 high-fidelity DNA polymerase (New England Biolabs Inc.). The total reaction volume of 50μL consisted of the 200ng/μL DNA extract ...
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bioRxiv - Molecular Biology 2020Quote: ... the first step PCR reaction was performed in a 12.5 μl mixture containing 1X OneTaq Hot Start Master Mix (NEB), 0.4 μM of each DHPV-U 1538F and DHPV-U 1887R primer and 20 ng of DNA template ...
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bioRxiv - Cell Biology 2020Quote: ... Integration at the correct locus was verified by colony PCR using LongAmp Taq 2X Master Mix (New England BioLabs), and the mto2 gene was sequenced in its entirety to verify the S338N or S338C point mutations.
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (Toxoplasma RH derivatives) was extracted for PCR using a Monarch Genomic DNA Purification Kit (New England Biolabs). Q5 Polymerase (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: All PCR reactions were carried out in 25 μL total reaction volumes using 2X Master Mix (New England Biolabs), oligonucleotide primers synthesized by Eurofins (Ebersberg ...
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bioRxiv - Cell Biology 2021Quote: ... Purified NLS-mNeptune2-T2A and ccz1 backbone PCR products were assembled together using NEBuilder HiFi Assembly cloning kit (NEB).
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs encoding human elongation factor eEF2 were amplified by PCR using Q5 High-Fidelity 2X Master Mix (NEB) with primers (forward primer ...
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bioRxiv - Genomics 2021Quote: ... the Hi-C library was amplified for about 10 cycles of PCR with the Q5 master mix (NEB, M0492L), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... the resulting supernatant was diluted 20-fold and used as template either for a 12.5 uL PCR reaction with Q5 Polymerase (NEB), or for a 10 uL qPCR reaction with the SsoAdvanced Universal SYBR Green 2X Supermix (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: PCR products were subject to in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB), which relies on the T7 RNA polymerase to initiate transcription from a T7 promoter sequence (present in our fragments) ...
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bioRxiv - Molecular Biology 2020Quote: ... All fragments used for the cloning step were amplified via PCR using Q5 high fidelity DNA polymerase (NEB #M0491S) (table S4 ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions for cloning were performed by using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Inc (NEB). All the products sequenced to verify the absence of any mutations (GATC-biotech).
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB). The pSG1164 plasmid integrates at the native locus of the corresponding gene by a single-crossover event ...
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bioRxiv - Microbiology 2022Quote: ... The resulting PCR product was integrated into the plasmid via the Gibson Assembly cloning system (New England Biolabs-NEB). The pSG1164 plasmid integrates at the native locus of the corresponding gene by a single-crossover event ...
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bioRxiv - Microbiology 2022Quote: ... and real-time PCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) on an ABI 7500 Fast RT-PCR system (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... The synthesized cDNAs were then used for quantitative PCR by using Luna Universal qPCR Master Mix (New England Biolabs). The following PCR Primers were used in this study ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified HTO sequencing libraries were then amplified by PCR (1μl clean HTO cDNA, 25μl 2X NEBNext Master Mix (NEB #M0541), 10 µM SI-PCR and D701or D704 primers performed dial out PCR (98°C (10 sec) ...
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bioRxiv - Biophysics 2022Quote: ... The 8C-8A(IL125) chimera cDNA construct was generated using the Phusion High-Fidelity PCR kit (New England BioLabs). All cDNAs were tagged on their carboxy terminus with Myc-DDK epitopes ...
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bioRxiv - Cell Biology 2022Quote: ... both the gene of interest and the vector were amplified by PCR using Q5 polymerase (New England Biolabs, US). After being digested with DpnI ...
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bioRxiv - Genomics 2022Quote: ... Each PCR was run using 12.5μL Q5® High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA), 2.5μL template DNA ...
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bioRxiv - Evolutionary Biology 2022Quote: 75ng of genomic DNA were used for PCR amplification in two 50µl reactions with 25µl 2xQ5 mix (NEB M0492), 2.5µM of each forward (FOS ...