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Citations for New England Biolabs :
5901 - 5950 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... an equimolar pool of all amplicons was prepared and purified using Monarch PCR & DNA Cleanup Kit (New England Biolabs). After DNA quantification using Qubit dsDNA HS Assay Kit and Qubit 2.0 fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... Pooled PCR products (1 μg) were digested with the NEBNext dsDNA fragmentase kit (New England BioLabs, Inc., Ipswich, MA). Fragmented DNA was end-repaired with the NEBNext End Repair Module (New England BioLabs ...
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bioRxiv - Biochemistry 2019Quote: ... PCR amplified ORF was cloned into a pGEX4T1 vector using NEB HF cloning kit (New England Biolabs, Inc. USA). E69A-TRAF6 was cloned using PCR based site-directed mutagenesis ...
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bioRxiv - Biophysics 2019Quote: Double-stranded DNA was synthesized through PCR amplification of a 1.565-kbp segment of the pBR322 plasmid (New England Biolabs), using forward and reverse primers conjugated with a 5’ biotin and a 5’ digoxigenin ...
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bioRxiv - Evolutionary Biology 2019Quote: A touchdown PCR protocol was followed (Korbie and Mattick 2008) and Phusion was used per the manufacturer’s instructions (NEB). PCR products that were approximately less than 3kb were TOPO cloned into the pCR4-Blunt vector (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... The purified PCR product was assembled into the linearized vector using Gibson Assembly (NEB Gibson Assembly 2x Master Mix), and transformed into E ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Purified first-round PCR products were combined with 10 μl NEBNext 2x High-Fidelity Master Mix (New England Biolabs), and 0.3 μM of each barcoding primer containing adapters and indexes to a total volume of 20 μl ...
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bioRxiv - Molecular Biology 2019Quote: ... bovis C9.1 gDNA by polymerase chain reaction (PCR) with Phusion High-Fidelity DNA Polymerase (New England BioLabs; Beverley, MA) using primers EAM6 and EAM18 ...
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DNA writing at a single genomic site enables lineage tracing and analog recording in mammalian cellsbioRxiv - Synthetic Biology 2019Quote: ... The initial PCR was performed for 25 cycles with Phusion HotStart Flex polymerase in GC buffer (New England Biolabs), each sample was purified with AMPure beads (0.9:1) ...
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bioRxiv - Biophysics 2019Quote: ... A 672bp length of DNA was prepared by PCR amplification of a section of lambda DNA (New England Biolabs) using a forward primer 5’-CGATGTGGTCTCACAGTTTGAGTTCTGGTTCTCG-3 and reverse primer 5’-GGAAGAGGTCTCTTAGCGGTCAGCTTTCCGTC-3’ purchased from Integrated DNA Technologies ...
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bioRxiv - Immunology 2019Quote: ... Full-length dram1 cDNA and dram1Δ19N cDNA was obtained by PCR amplification using Phusion High-Fidelity DNA Polymerase (M0530S, New England Biolabs). The following primers were used ...
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bioRxiv - Genomics 2020Quote: ... Primer mixes were diluted 1:25 and 1μl of each mix was used for overlap-extension PCR using Phusion (NEB). Four 50μl reactions for each mix were performed using cycling conditions 98°C 1min ...
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bioRxiv - Microbiology 2021Quote: ... The resulting backbone was subsequently gel-purified and assembled with the PCR-amplified fragment of parBΔCTD using a 2x Gibson master mix (NEB). Gibson assembly was possible owing to a 23-bp sequence shared between the NdeI-HindIII-cut pET21b backbone and the PCR fragment ...
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bioRxiv - Microbiology 2021Quote: ... Chimeric GBP sequences were produced by joint PCR using a Phusion® High-Fidelity DNA polymerase (New England BioLabs) and purified from agarose gel using NucleoSpin® Gel and PCR clean up kit (MACHEREY-NAGEL) ...
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bioRxiv - Bioengineering 2020Quote: ... The oligopool was amplified to create double stranded DNA: PCR conditions: 0.02 U/μL Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs), 1X Q5 reaction buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The first PCR specifically amplified the region of DNA encoding for Gp2: PCR conditions: 0.02 U/μL Q5 High-Fidelity DNA Polymerase (New England Biolabs), 1X Q5 reaction buffer ...
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bioRxiv - Bioengineering 2021Quote: ... 20 ng of extracted plasmid DNA was added to a 50 µL PCR reaction with Q5 DNA polymerase (NEB) for 16 cycles annealing at 53 °C ...
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bioRxiv - Biochemistry 2021Quote: ... and then digested PCR product and CIP-treated vector backbone were ligated with T4 DNA ligase (New England Biolabs). Ligation reactions were transformed into chemically competent E ...
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bioRxiv - Biophysics 2021Quote: The 3352 bp central fragment was produced by PCR with Taq DNA polymerase (New England BioLabs, Ipswich, MA, USA) from the template plasmid pYY_I1_400_BstEII[12] with forward (5-atgttcGGGCCCagtaacccgtatcgtgagc ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA was amplified as described before with NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs). The PCR amplified product was cleaned twice with (0.9X ...
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bioRxiv - Neuroscience 2019Quote: ... pellet samples were collected from the qRT-PCR plate and mixed with Purple Gel Loading Dye (BioLabs, Cat#B7025S) to be load on 1% agarose gel ...
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bioRxiv - Plant Biology 2021Quote: PCR amplifications were performed using Phusion® High-Fidelity DNA Polymerase (New England Biolabs, Massachusetts, USA, Cat. No. M0530). All cloning was done using In Fusion® HD Cloning kit (Takara ...
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bioRxiv - Biochemistry 2021Quote: The Q5 Site-Directed Mutagenesis PCR reaction was performed on 20 ng of the RIPK3 vector according to the supplier’s instruction (New England Biolabs) using primers and annealing temperatures (Tm ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 2 μl of the circularized product was then used for PCR amplification using Phusion High-Fidelity DNA Polymerase (NEB) for a maximum of 16 cycles ...
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bioRxiv - Bioengineering 2021Quote: 2 μl of genomic DNA was used to amplify strain barcodes by PCR (Q5 NEB master mix, 22 cycles) using primers containing sequence-optimized spacers to maximize nucleotide diversity in Illumina sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... PCR conditions for ACMV were similar except the annealing temperature was 55°C and Hot Start Taq Polymerase (NEB optimized for large product sizes was used ...
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bioRxiv - Plant Biology 2021Quote: ... High fidelity PCR was conducted on the abcg43-1 line using the Q5 High-Fidelity 2X Master Mix (NEB). PCR products were purified and extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 96-well plates were centrifuged again at 3000 x g for 10 min and 1 µL of the supernatant was used in 12.5 µL PCR reactions with Taq polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were then incubated at 65°C for 30 minutes in the presence of BsmI restriction enzyme (NEB). The resulting DNA fragments were separated by 1.5% agarose gel electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... Restriction digest of EtMIC3 PCR product was performed using Bam HI and Eco RI restriction enzymes (New England Biolabs) to extract antigen coding sequence for cloning into pYD1 plasmid vector ...
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bioRxiv - Microbiology 2021Quote: ... Assembly of the RNA coding sequence and template was accomplished by PCR using Q5 High-Fidelity DNA polymerase (NEB). Finally ...
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bioRxiv - Microbiology 2021Quote: ... and PCRs requiring proofreading were performed with the Q5® High-Fidelity DNA Polymerase (New England BioLabs, Evry, France) as described by the manufacturers ...
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bioRxiv - Microbiology 2019Quote: ... The two PCR products were then assembled into the vector using the NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Molecular Biology 2020Quote: ... ORC2 sgRNA resistant synonymous mutations were introduced to ORC2 by PCR mutagenesis using Phusion high fidelity DNA polymerase (NEB). Guide RNA resistant ORC2 (ORC2gr ...
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bioRxiv - Neuroscience 2019Quote: ... The 388bp PCR amplicon was digested with 20U/µL EcoRV restriction enzyme prepared in 10X NEB 3.1 buffer (New England Biolabs) and incubated at 37°C for 60min to distinguish between the wild-type (388bp ...
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bioRxiv - Plant Biology 2020Quote: ... The 839-bp PCR product was digested with SapI and XhoI and ligated into SapI–XhoI-digested pTYB11 (NEB), generating pMS451 ...
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bioRxiv - Plant Biology 2020Quote: All DNA fragments generated by PCR for cloning were amplified using the Q5 High-Fidelity DNA polymerase (NEB, USA), as indicated by manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... A 470 bp PCR product surrounding the UL23 cut site was amplified using Phusion high-fidelity polymerase (NEB, USA) and column purified (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2020Quote: ... the targeted genomic sites were amplified by PCR with Phusion Hot Start Flew 2x Master Mix (New England BioLabs) and primers with partial Illumina sequencing adaptors ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were performed using Q5© high-fidelity DNA polymerase [2,000 units/ml] (New England Biolabs, Germany) in triplicates ...
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bioRxiv - Microbiology 2019Quote: ... A 1138 bp and a 2167 bp product were generated by PCR using Q5 polymerase (NEB, Ipswich, MA, USA) and HE10DO DNA as template ...
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bioRxiv - Microbiology 2020Quote: ... Phage genes were amplified from Butters genomic DNA by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs). All PCR products contained the entire 179bp between gene 29 and gene 30 (containing the endogenous promoter and ribosome binding site [RBS] ...
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bioRxiv - Cell Biology 2021Quote: ... The targeting genomic PCR in CCR5-mutated clones was performed using Q5 Hot Start High Fidelity DNA polymerase (NEB) with the following primers ...
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bioRxiv - Biophysics 2020Quote: Different lengths of linear DNA substrates were generated by PCR using the Hot start Phusion polymerase (New England Biolabs), as described previously based on the pCERoriD (Chisty et al. ...
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bioRxiv - Biophysics 2020Quote: The bare (non-methylated) PCR constructs were also methylated enzymatically by CpG methyltransferase M.SssI (New England Biolabs, Ipswich, MA). To ensure complete methylation ...
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bioRxiv - Genomics 2021Quote: ... 10°C hold in one cycle) by adding 25 µl of 2X PCR master mix (New England Biolabs, M0541S) and 0.8 µl of Ad 2.X reverse primer ...
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bioRxiv - Biophysics 2022Quote: The long DNA linkers containing a 5’ overhang on one end and digoxigenin on the other end were prepared by PCR 68,69 using Q5 polymerase (NEB). Lambda phage DNA (Thermofisher ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA fragments were amplified by PCR using Q5® High-Fidelity DNA polymerase (New England Biolabs Inc., USA).
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bioRxiv - Neuroscience 2022Quote: ... we added 1μL unique barcoded N5&N7 (0.5 µM final concentration) and 10 μL NEBNext High-Fidelity 2x PCR Master Mix (NEB). PCR cycling conditions were as follow ...
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bioRxiv - Cancer Biology 2022Quote: ... The PCR product was then gel purified with NEB Monarch® DNA Gel Extraction Kit (NEB, Cat No. T1020). Alongside pLentiCRISPRv2 (Addgene ...