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Citations for New England Biolabs :
6251 - 6300 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: The PCR reaction was motivated in 30 μL reaction systems after mixing 15μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers labelled with specific barcodes ...
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bioRxiv - Developmental Biology 2019Quote: ... and the bar-1 locus was genotyped for successful co-conversion by PCR amplification with OneTaq polymerase (New England Biolabs) using primers that annealed to the genomic DNA surrounding the insertion point ...
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bioRxiv - Biochemistry 2019Quote: The required coding region was amplified from the H6-TtBac construct by PCR and was cloned into the plasmid pHis17 using Gibson Assembly (New England Biolabs), resulting in a C-termin tag ...
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bioRxiv - Biochemistry 2019Quote: ... Genes encoding for each nanobody were amplified using colony PCR and cloned into the pHEN6c expression vector (VIB) using Gibson Assembly (New England Biolabs). The resulting constructs using pHEN6c encoded for PelB(leader)-nanobody-SSHHHHHH proteins.
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bioRxiv - Biochemistry 2019Quote: The required coding region was amplified from the H6-TtBac construct by PCR and was cloned into the plasmid pHis17 using Gibson Assembly (New England Biolabs), The plasmid was used to transform C41(DE3 ...
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bioRxiv - Plant Biology 2019Quote: The promoter region and full-length CKX2I or coding DNA sequence (CDS) were amplified by PCR (Table S2) from genomic DNA or cDNA using Q5 High-Fidelity DNA Polymerase (NEB) and cloned either alone or under of the 35S promoter together with GFP and mScarlet-i into pPLV03 or pGEX5x3 using Gibson Assembly (NEB) ...
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bioRxiv - Genomics 2020Quote: ... was transcribed from a sgRNA-coding PCR product with a 5′ T7 promoter sequence using HiScibe T7 Quick High yield RNA Synthesis kit (NEB). The transcription was performed at 37 °C overnight and then purified by phenol ...
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bioRxiv - Cancer Biology 2019Quote: ... 200 ng of purified PCR product were denatured and re-annealed in NE Buffer 2 (New England BioLabs, Cat. # B7002S). T7 endonuclease 1 (New England BioLabs ...
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bioRxiv - Molecular Biology 2019Quote: ... Final libraries for each ChIP were produced using 150-200 ng of purified cDNA in a PCR reaction (High-Fidelity 2x master mix, New England BioLabs) for 8 cycles with 0.2 μM primers that carried a second 8bp barcode sequence ...
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bioRxiv - Microbiology 2019Quote: ... 500 bp upstream and 500 bp downstream of the gene to be deleted were amplified by overlapping PCR with Q5 high fidelity DNA polymerase (NEB) using primers listed in Supplementary Table 1 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the editing domain-coding segment of leuS gene was PCR amplified by using Phusion® High-Fidelity DNA Polymerase (NEB), primers 1 and 2 ...
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bioRxiv - Synthetic Biology 2019Quote: ... the original pSJ051 plasmid was mutated at the RBS upstream of triA (changed from AGGAGA to AGAAGA) by inverse PCR (Liu and Naismith, 2008) using Q5 DNA polymerase (NEB) using primers D101108989 ...
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bioRxiv - Genetics 2019Quote: The TDP-43 library was then prepared for deep sequencing by PCR amplification in two steps using Q5 High-Fidelity DNA Polymerase (NEB). In step 1 ...
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bioRxiv - Genetics 2019Quote: ... Genomic regions of interest (spanning 2 kb upstream of the start codon ATG and the full coding sequence excluding stop codon) were amplified by PCR with Phusion DNA polymerase (NEB). Promoter::CDS amplicons were cloned via KpnI/BamHI restriction sites into a pGreen-IIS backbone (Basta resistance ...
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bioRxiv - Plant Biology 2019Quote: ... All standard PCR reactions were performed with a 57°C annealing temperature using Taq polymerase with Standard Taq Buffer (New England Biolabs).
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bioRxiv - Microbiology 2019Quote: ... The full-length gene (with terminal NdeI and HindIII cut sites) was synthesized by overlap-extension PCR using Q5 polymerase with high GC buffer (NEB) and primers 979 and 987 (Supplemental Table 2) ...
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bioRxiv - Genomics 2019Quote: ... solution was diluted 1:20 and 5 μL used in a standard 50 μL PCR reaction using Q5 enzyme (NEB). PCR products were Sanger sequenced and analyzed using SnapGene to identify SNP corrected clones (7/192) ...
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bioRxiv - Biochemistry 2019Quote: ... Quantification of the ratio of MS2-tagged rRNA to WT rRNA was done via semi-quantitative PCR with Q5 DNA polymerase (NEB). Primers MS2_quant_F/R bind outside the MS2 tagged region of 23S cDNA ...
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bioRxiv - Biochemistry 2019Quote: ... fused to the 3’ end of the nucleotide sequence encoding full length Ypt7 was amplified by PCR from pET-19 Ypt7-tm (a kind gift from C Ungermann) with the Phusion high-fidelity DNA polymerase (NEB). The DNA fragment was cloned into BamHI and SalI digested pMBP-parallel1 vector (Sheffield et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... 1ul of cDNA was used for the first PCR (20ul volume) performed with Taq DNA polymerase (New England BioLabs, M0273) and primers listed in Table S4 ...
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bioRxiv - Synthetic Biology 2019Quote: ... All the PCR reactions were performed according to the Phusion® High-Fidelity DNA Polymerase (New England Biolabs, MA, USA) protocol using 0.05 Units of Phusion and 100 µM dNTPs in a total volume of 25 µl.
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bioRxiv - Molecular Biology 2019Quote: ... a ~200 bp amplicon was generated from 1×106 template molecules by PCR in 22 cycles using Phusion polymerase (NEB), purified with the QIAquick gel extraction kit ...
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bioRxiv - Molecular Biology 2019Quote: A 1 kb long DNA fragment with biotin molecules attached to the 5′ termini (dibiotin-DNA) was generated by PCR amplification using Q5 High-Fidelity Polymerase (NEB), with pAM075 as a template and the 5′-biotinylated primer pair oAM091/oAM092 ...
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bioRxiv - Genomics 2020Quote: We used the prepared DNA of each clone and the wild type gene for amplification by PCR with Q5 polymerase (NEB) using primers which included the minION barcodes adapters ...
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bioRxiv - Biochemistry 2020Quote: ... The region harboring the randomized hexamer and flanking constant tags was amplified from 1 µg ssDNA for 5 cycles using the Q5 Hot Start High-Fidelity PCR Master Mix (New England Biolabs) and the following primers ...
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bioRxiv - Genetics 2020Quote: ... Oligo pairs were mixed at an equimolar ratio in PCR tubes containing T4 Ligation buffer and T4 polynucleotide kinase (NEB) for 5’ phosphorylation of oligos ...
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bioRxiv - Genomics 2021Quote: ... Shotgun sequencing was performed using a PCR-free DNA library preparation (NEBNext Ultra II DNA Library Prep Kit, New England Biolabs). Libraries were paired end 150 bp sequenced with Illumina HiSeqX ...
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bioRxiv - Genomics 2021Quote: ... and on the other side a primer that targets a region introduced through the library preparation called “Read 1” (18 cycles using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs)) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and re-amplified the size-selected libraries using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Canada). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... vector was generated by replacing the Ef1a promoter with a CMV promoter cassette in pLV-Ef1a-Cre-U6-sgRNA(MS2) using PCR and HiFi assembly (NEB). The pLV-CMV-CreERT2-P2A-[puroR/zeoR/hygR]-U6-sgRNA(MS2 ...
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bioRxiv - Cancer Biology 2021Quote: ... vector was generated by replacing the BleoR cassette with Cre recombinase from the pBS513 EF1alpha-Cre vector in the plenti sgRNA(MS2)_zeo backbone using PCR and HiFi assembly (NEB). One BsmBI site in the Cre cassette was destroyed through targeted mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB ...
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bioRxiv - Immunology 2021Quote: ... cDNA was generated using the SuperScript IV reverse transcriptase kit (TFS) and PCR carried out using Q5 High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The first 60 μL PCR reaction (also run as three parallel 20 μL reactions) included 6 μL of 10X ThermoPol Taq buffer (NEB), 0.96 μL of 5 μM forward primer (0.08 μM final) ...
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bioRxiv - Cell Biology 2021Quote: ... which were then digested by SacI/MscI and ligated with SacI/MscI-digested second PCR fragment by T4 DNA ligase (M0202S; NEB). Linear cc2 constructs were obtained by SacI/KpnI digestion of the resulting plasmids and transformed into desired strain for cc2 insertion.
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bioRxiv - Plant Biology 2020Quote: ... the genomic regions containing pre-miR775a and the GALT9 coding region were PCR amplified using the Pfusion DNA polymerase (New England Biolabs) and primers listed in Supplemental Table 2 ...
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bioRxiv - Plant Biology 2020Quote: T-DNAs expressing four sgRNAs were assembled from BsaI-linearized vectors and three PCR fragments using an NEBuilder kit (E2621, New England Biolabs). Appropriate PCR fragments were generated in three- or four-primer reactions similar to the ones described above ...
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bioRxiv - Microbiology 2021Quote: PCR reactions to amplify the trailer sequences from the circularized cDNA using Q5 High-Fidelity DNA Polymerase (New England Biolabs) using 60 ng of circularized template were then set up with the following parameters ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR fragment and the NdeI-NheI-cut pMT571 were assembled together using a 2x Gibson master mix (NEB). Gibson assembly was possible owing to a 23 bp sequence shared between the two DNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... Subcloned cells were screened for correct targeting by PCR amplification and restriction enzyme digestion (MCM10 exon 3, Hpy199III (NEB R0622); CDC45 exon 3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... parts were domesticated removing BpiI and BsaI sites by PCR-based mutagenesis and cloned into corresponding vectors by GG cloning using either BsaI (NEB) or BpiI (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The Purified PCR product was treated with Cre recombinase as described in the instructions (M0298, New England Biolabs, Ipswich, MA). The CaMK1 floxed PCR product and control plasmid (pLOX ...
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bioRxiv - Cell Biology 2021Quote: ... The final amplicon was purified from genomic DNA using a Monarch PCR and DNA Cleanup Kit (New England Biolabs T1030) and quantified with a Qubit 2.0 Fluorometer ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA isolated from Xanthomonas albilineans CFBP7063 was PCR amplified using Q5 Hot Start High-Fidelity 2X Master Mix (NEB) and cloned into pET28a using Gibson Assembly ...
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bioRxiv - Microbiology 2020Quote: ... ∼1.2 kb upstream and downstream of the tcdR gene were PCR amplified with Phusion High-Fidelity DNA polymerase (NEB M0530S). All primers used for construction of the mutagenesis vector and for PCR screening of transconjugants can be found in Supplemental Table 1 ...
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bioRxiv - Biochemistry 2020Quote: ... Cas9 variant plasmids were constructed via ATW PCR and a KLD reaction and/or via DNA assembly using New England Biolabs (NEB) HiFi DNA Assembly (NEB E5520S) ...
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bioRxiv - Biochemistry 2020Quote: DNA fragments containing sgRNA sequences were amplified from isolated genomic DNA by two rounds of PCR using NEBNext Ultra II Q5 master mix (NEB). For the first round of PCR ...
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bioRxiv - Biochemistry 2020Quote: ... Modification of the coding sequence of the multibasic S1/S2 cleavage site PRRAR to PGSAS or to a single R was carried out by PCR mutagenesis using Q5 polymerase (New England Biolabs). Introduction of cysteine crosslinks and modification of residues 986 and 987 were carried out using Q5 polymerase PCR with primers containing desired substitutions ...
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bioRxiv - Bioengineering 2021Quote: ... 1 μl of cDNA was amplified by PCR with primers that amplify about 300-600 bp surrounding the sites of interest (outside the length of the antisense domain) using OneTaq PCR Mix (NEB). The numbers of cycles were tested to ensure that they fell within the linear phase of amplification ...
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bioRxiv - Bioengineering 2021Quote: ... were either synthesized or PCR-amplified and then cloned into the px552-U6-CAG-mCherry plasmid using an NEBuilder HiFi DNA assembly kit (NEB). The AAV vectors were then sent to VectorBuilder Inc (Chicago ...