Labshake search
Citations for Illumina :
401 - 418 of 418 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using the New England Biolabs NEBNext rRNA depletion kit (Bacteria) and NEBNext Ultra II Directional RNA library prep kit (Illumina, San Diego, CA). The libraries were quantified by KAPA qPCR library quantitation assays and sequenced paired-end using MiSeq platform (Illumina ...
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bioRxiv - Genetics 2020Quote: ... sequencer-ready fragments were size selected (∼200-500 bp) and the RAD-seq libraries sequenced on a Genome Analyzer II (Illumina, San Diego, CA), paired-end 2 x 100 bp mode ...
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bioRxiv - Developmental Biology 2021Quote: ... The nuclei were suspended in a transposition mix (TD Tagment DNA Buffer, Illumina Catalog No. 15027866, and TDE1 Tagment DNA Enzyme, Illumina Catalog No. 15027865), and incubated at 37°C to generate DNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-qPCR gene expression quantifications were performed using AceQ qPCR SYBR Green Mix (Vazyme Biotech) on Eco Real-Time PCR System (Illumina, San Diego, CA). RNA expression was normalized to a geometric mean of two reference genes ...
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bioRxiv - Immunology 2021Quote: ... Chromatin fragments bound to magnetic beads were resuspended in 30 μl Tagmentation mix (14 μl H2O, 15 μl Tagment DNA Buffer [Illumina, order no. 15027866], 1 μl Tagment DNA Enzyme [Illumina, order no. 15027865]) and incubated for 10 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Post-ligation cleanup proceeded according to Illumina’s instructions with 110 µL Sample Purification Mix from the TruSeq Methyl Capture EPIC LT Library Prep Kit (Illumina catalog # FC-151-1002). After purification ...
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bioRxiv - Genetics 2020Quote: ... and the nuclei was resuspended with Tn5 transposase reaction mix (25◻μl 2◻×◻TD buffer, 2.5◻μl Tn5 transposase and 22.5◻μl nuclease-free water) (Illumina) at 37◻°C for 30 min ...
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bioRxiv - Genomics 2022Quote: ... spun at 300G for 12 min at 4 ºC and resuspended in 20 uL transposition mix: 1X TD buffer and 2 uL of TDE1 (Illumina #FC-121-1030), 0.01% Digitonin in DMSO ...
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bioRxiv - Genomics 2020Quote: Fragmented cDNA Sequencing libraries were generated from 100ng of Total RNA using NEBNext Ultra II with polyA isolation module (Illumina, San Diego, California, USA) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing libraries were prepared using NEBNext Ultra II directional RNA library kit and paired-end sequenced (150bp) in an Illumina HiSeq3000 (Illumina Inc., San Diego, USA) instrument ...
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bioRxiv - Genetics 2019Quote: ... The coding and splice-site regions of genes susceptible to hereditary arrhythmia and cardiomyopathies(10) were sequenced for probands II:3 and III-7 using Illumina HiSeq2500 Analyzer (Illumina, San Diego, CA, USA)(11) ...
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bioRxiv - Immunology 2021Quote: ... Library preparation was done using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with poly-A selection (Illumina, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Amplicons of the V3-V4 region of the 16S rRNA gene and the and the ITS1 region and library prep were generated by Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina, San Diego, CA, USA). The products of the library prep were validated by an Agilent Tapestation D1000 (Santa Clara ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA libraries were constructed from 1 μg/sample of total RNA using library preparation kits (NEBNext Ultra II RNA Library Prep Kit for Illumina 7770L, NEBNext Multiplex Oligos for Illumina E7335S, NEBNext Poly(A) mRNA Magnetic Isolation Module E7490L ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of total RNA was used for intact RNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760, New England BioLabs). To obtain sufficient RNA for the analysis of oral samples ...
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bioRxiv - Genomics 2019Quote: ... and then incubated with 5 μl of Tn5 Transposase of TTE Mix V50 in 45 μL of 1 x TTBL buffer (TruePrep® DNA Library Prep kit V2 for Illumina®, Vazyme, China) at 37°C for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Transposition was performed directly on nuclei using 25 µl tagmentation reaction mix (Tagment DNA Buffer #15027866, Tagment DNA Enzyme #15027865 from Illumina Tagment DNA kit #20034210). Tagged DNA was subjected to PCR amplification and library indexing ...
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bioRxiv - Immunology 2023Quote: ... 3mM MgCl2) on ice and resuspended in 10μl of transposition mix (1X TD Buffer from Illumina, Transposase from Illumina, 0,01% digitonin, 0,1% Tween-20). Tagmentation was performed on a thermomixer at 1000rpm 37°C during 30min ...