Labshake search
Citations for Illumina :
351 - 400 of 418 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... ChIP samples were analyzed by qPCR or subjected to library preparation according to the manufacturer’s protocol (NEBNext Ultra II DNA Library Prep Kit for Illumina, NEB) using Dual Index Primers (NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... DNA was measured again with Qubit dsDNA HS Assay Kit and 65ng of mononucleosomal purified DNA was used for NEBNext Ultra II DNA Library Prep Kit (Illumina) with size selection ...
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bioRxiv - Genomics 2022Quote: ... where polyA libraries were prepared with the NEBNext Ultra II mRNA-Seq protocol and sequenced on a NovaSeq sequencer (Illumina) to obtain ∼60 million PE150 pairs per sample.
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bioRxiv - Plant Biology 2023Quote: ... cDNA libraries were constructed from 1 μg/sample of total RNA using library preparation kits (NEBNext Ultra II RNA Library Prep Kit for Illumina 7770L ...
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bioRxiv - Microbiology 2023Quote: The extracted DNA samples were prepared for Illumina next-generation sequencing using the NEBNext Ultra II FS DNA Prep kit from Illumina, part number E7805 ...
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bioRxiv - Genetics 2023Quote: ... Batch 1 sequencing libraries were prepared by a commercial vendor (Azenta/Genewiz) using the NEBNext Ultra II RNA Library Prep Kit (Illumina) and sequenced using 2 x 150 bp paired end reads ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed using NEB polyA RNA ultra II and subsequently subjected to 150 cycles of sequencing on NovaSeq-6000 (Illumina). Adapters were trimmed using Cutadapt (v2.3) ...
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bioRxiv - Genomics 2023Quote: ... we prepared stranded cDNA libraries for 909 samples from 695 sepsis patients using NEB Ultra II LIbrary Prep kits (Illumina) with NEBNext Poly(A ...
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bioRxiv - Molecular Biology 2024Quote: ... Extracted total RNA was send to Azenta Life Sciences for library preparation (sRNA: NEBNext Small RNA Library Prep Set for Illumina; mRNA: NEBNext Ultra II RNA Library Prep Kit for Illumina) and paired-end Illumina sequencing (NovaSeq 2×150bp).
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bioRxiv - Microbiology 2021Quote: ... Each PCR product of the appropriate size was purified and quantified using a Qubit fluorometer and then added to a master pool of DNA for 250-bp nucleotide paired-end read assembly using the HiSeq 2500 genome analyzer (Illumina HiSeq 2500, USA).
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 µL of 10 µM primer mix (515F forward and 806R reverse rRNA gene V4 primers with Illumina MiSeq adaptors), and 30 ng of sample DNA ...
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bioRxiv - Genomics 2019Quote: ... and the transposition was performed by resuspension of nuclei in 50 µL of Transposition Mix containing 1X TD Buffer (Illumina) and 2.5 µL Tn5 (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Chromatin fragments bound to magnetic beads were resuspended in 30 μl Tagmentation mix (14 μl H2O, 15 μl Tagment DNA Buffer [Illumina, order no ...
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bioRxiv - Neuroscience 2020Quote: ... RNAseq libraries were made using the TruSeq Stranded Total RNA Library prep kit with rRNA removal mix Gold (Illumina, 20020598). Libraries were quantified and normalized using the KAPA Library Quantification kit for Illumina Platforms (KAPA Biosystems ...
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bioRxiv - Developmental Biology 2019Quote: ... lysed and mixed with 50 µl of transposition reaction mix containing Tagment DNA Enzyme (Nextera DNA Library Preparation Kit, Illumina) and 0.2% Digitonin ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclear pellets were then resuspended in 50 μl transposition reaction mix (25 μl TD buffer (Nextera DNA Library Prep Kit, Illumina), 2.5 μl TDE1 enzyme (Nextera DNA Library Prep Kit ...
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bioRxiv - Genetics 2020Quote: ... We then resuspended the nuclear pellet in 50 μl of transposition reaction mix (25 μl Buffer TD, 2.5 μl TDE1 (Illumina 15028212); 7.5 μl water ...
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bioRxiv - Immunology 2022Quote: ... cDNA libraries were amplified 12 cycles of PCR with Terra PCR Direct polymerase Mix in the presence of i5 or i7 PCR primers (Illumina) to add sample specific indexes ...
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bioRxiv - Molecular Biology 2022Quote: ... The generated libraries were amplified using the KAPA HiFi Hotstart Ready Mix and Nextera Index Kit 1 (i7) and 2 (i5) primers (Illumina). Amplified libraries were purified using a 0.65x AMPure XP beads (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Purified DNA was further amplified by PCR using Kapa Hifi Hotstart Ready Mix with Nextra XT Index Primers from Nextra XT Index Kit (Illumina). The PCR condition was ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR reactions were performed using the Thunderbird® SYBR qPCR mix (TOYOBO) and Eco Real-Time PCR System (Illumina). The PCR primers used are listed in Supplementary Table S8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were resuspended in a transposition reaction mix prepared using the Illumina Tagment DNA Enzyme and Buffer Kit (Illumina, 20034197) and incubated at 37 °C for 30 minutes on thermomixer at 1,000 rpm to allow for transposition-based DNA fragmentation and adapter ligation ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were mixed with 50 µl of transposition reaction mix containing Tagment DNA Enzyme 1 (Nextera DNA Library Preparation Kit, Illumina). The transposition reaction was carried out at 37°C and 600rpm for 30 min ...
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bioRxiv - Genomics 2023Quote: Pelleted nuclei were gently resuspended in a 50 μL transposition reaction mix composed of 25 μL 2× TD Buffer (Illumina), 2.5 μL Tn5 Transposes (Illumina ...
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bioRxiv - Immunology 2024Quote: ... After centrifugation at 500ξ g for 5min at 4°C, the cell pellets were resuspended in tagmentation-mix (containing Tagment DNA buffer, Tagment DNA Enzyme (Illumina), Digitonin (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: 20-50ng PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) ...
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bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: 20-50ng PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) total 20µl ...
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bioRxiv - Neuroscience 2024Quote: ... sequencing adaptors P5 and P7 were introduced following the 10xGenomics dual-index setup: Eluted PCR product was added to library PCR mix (50% KAPA, 10% Betaine, 20% primer Dual index (Illumina)) ...
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bioRxiv - Genomics 2020Quote: The immunoprecipitated sample and the input were subjected to Nextera library preparation as described by the manufacturer (NEBNext Ultra II DNA library Prep Kit for Illumina, NEB). The quality and size of libraries and chromatin shearing fragments were checked by Agilent Bioanalyzer using Agilent DNA High Sensitivity Chips (Agilent Technologies) ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... mice were injected intraperitoneally with 150 mg kg−1 D-luciferin (Caliper Life Sciences) in 100 μL and mice imaged in vivo using an IVIS imaging chamber (IVIS Illumina II). Luminescence measurements (photons/second/cm2 ...
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bioRxiv - Microbiology 2022Quote: ... Paired-end libraries were constructed using the Illumina Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina, Eindhoven, Netherlands). Primers used for bacterial amplification were Bac341F (5’-CCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Plant Biology 2023Quote: ... from 100 to 1000 ng of total RNA using a stranded kit (Illumina TruSeq Stranded Total RNA Kit or NEB Next Ultra™ II Directional RNA Library Prep Kit for Illumina). The resulting data files contained paired end sequences (150 bp ...
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bioRxiv - Cancer Biology 2023Quote: Mice were injected intraperitoneally with 150 mg kg-1 D-luciferin (Caliper Life Sciences) in 100 μL and mice imaged in vivo using an IVIS imaging chamber (IVIS Illumina II). Luminescence measurements (photons per second per cm2 ...
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bioRxiv - Plant Biology 2024Quote: ... DNA eluted from Chromatin-immunoprecipitated was prepared for library construction by using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads according to standard operating procedures and then sequenced using HiSeq2000 (Illumina). Paired-end raw reads were first mapped to the Arabidopsis genome (TAIR10 ...
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bioRxiv - Microbiology 2024Quote: ... mRNA was used to generate the cDNA library according to the NEBNext® UltraTM II RNA Library Prep Kit protocol (Illumina), which was then sequenced using the Illumina HiSeq 2000 system ...
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bioRxiv - Genetics 2021Quote: 5× HOT FIREPol® EvaGreen® qPCR Mix Plus with ROX (Solis Biodyne) and an Eco Real-Time PCR System (Illumina) were used for qPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was then mixed and incubated at 37°C for 1h min with the transposition reaction mix prepared using Illumina Tagment DNA Enzyme and Buffer kits (Illumina, 20034197). DNA was then purified using DNA Clean and Concentrator kit (Zymo ...
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bioRxiv - Cell Biology 2024Quote: ... Prime Finish Mix provided in the TruSeq Stranded Total RNA Library Prep Gold Kit (Cat# 20020599, Illumina, San Diego, CA, USA) used for completing library preparation ...
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bioRxiv - Developmental Biology 2021Quote: The resulting 79,472,462 paired end reads (NCBI PRJNA690950) were combined with an older library (16,225,145 paired end reads sequenced on an Illumina GA II; NBCI SRR1145735) for annotation of protein coding regions.
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bioRxiv - Neuroscience 2021Quote: ... Hippocampus RNA-seq libraries were prepared in accordance with New England Biolab protocols (NEBNext_ Ultra II RNA library Prep kit for Illumina, NEB, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... libraries for sequencing were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® following the manufacturer’s instructions (Illumina). Quality of all RNA and library preparations were evaluated with BioAnalyser 2100 (Agilent) ...
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bioRxiv - Microbiology 2022Quote: ... The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat. 15052163). The PCR products were purified using 1.8× beads ...
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bioRxiv - Microbiology 2023Quote: ... Preparation of cDNA fragment libraries was performed using the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (Illumina). Sequencing was carried out on the Illumina NovaSeq platform (10 M paired-end reads ...
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bioRxiv - Genomics 2023Quote: ... single-indexed and dual-indexed DNA libraries were generated using the NEBNextUltra II DNA Library Prep kit from Illumina (New England Biolabs). Single-indexed libraries were sequenced 50 nt single end reads on a HiSeq 2500 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... Approximately 300 ng of RNA was used for library prep (NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina) following standard kit procedure ...
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bioRxiv - Neuroscience 2021Quote: ... Immediately following the cell preparation, the pellet was resuspended in the transposase reaction mix (25 μL 2× TD buffer, 2.5 μL transposase (Illumina, San Diego, USA) and 22.5 μL nuclease-free water) ...
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bioRxiv - Genomics 2019Quote: ... Second, the pellet was resuspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl transposase (Illumina, FC-121-1030) and 22.5 μl nuclease-free water) ...
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bioRxiv - Genomics 2022Quote: ... and nuclei pellet was resuspended in 50 μL of the transposase reaction mix including 25 μl of 2xTD buffer (Illumina, cat#15027866), 2.5 μl of transposase (Illumina ...
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bioRxiv - Physiology 2024Quote: ... tagmented DNA was amplified by PCR in a reaction mix (5 µL DNA, 2.5 µL of 25 μM forward primer (Nextera/Illumina i5 adaptors (Illumina)) ...
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bioRxiv - Cell Biology 2022Quote: ... 1,500ng of RNA at 50ng/μl was processed using Ribo-Zero Gold purification (QIAseq FastSelect -rRNA HMR Kit #334387) and NEBNext® Ultra™ II RNA Library Prep Kit (Illumina #E7770L). cDNA was sequenced using paired-end 150bp chemistry on a HiSeq 4000 instrument (Illumina ...