Labshake search
Citations for Illumina :
251 - 300 of 418 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL of transposase mix with 2.5 μL of Tn5 transposase (Illumina, San Diego, CA) were added and incubated for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and sequenced on an Illumina NextSeq 500 system using the NextSeq 500/550 Mid Output Kit v2.5 (#20024904, Illumina; 150 cycles, paired-end run 2x 75 bp) with an average of 1×107 reads per RNA sample.
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bioRxiv - Cell Biology 2022Quote: ... Sequencing libraries for osteoclasts were prepared using NEBNext Ultra II Directional RNA Library Prep kit (Illumina). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... The sequencing libraries were made using NEBNext® Ultra™ II DNA Library Prep Kit (Illumina) according to the manufacturer’s instructions and submitted to Novogene for NGS with PE150.
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bioRxiv - Neuroscience 2024Quote: ... followed by NEBNext directional Ultra RNA II Library preparation and sequencing on the NextSeq500 platform (Illumina) using the high output version 2 kit with 75 cycles ...
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bioRxiv - Biochemistry 2023Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library prep kit from Illumina according to the manufacturer’s instructions and sequenced as PE150 reads on an Illumina NovaSeq 6000 ...
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bioRxiv - Genetics 2021Quote: ... and then resuspended in tagmentation reaction mix for 30 min (2.5uL TD1, 1X TD Buffer, Illumina Nextera DNA ...
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bioRxiv - Genetics 2019Quote: ... Cells were incubated in Tagmentation mix (Tagmentation DNA buffer Illumina 15027866; Tagmentation DNA enzyme Illumina 15027865) for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... The libraries then were amplified using PCR Enhancer mix and primer cocktail (Illumina, FC-121-4001) for 12 cycles ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were then resuspended in 50 μl transposase reaction mix (1x Tagment DNA buffer (Illumina 15027866), 2.5 μl Tagment DNA enzyme I (Illumina 15027865) ...
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bioRxiv - Genomics 2021Quote: ... Pelleted nuclei were resuspended in 50 µl transposase reaction mix (1x Tagment DNA buffer (Illumina 15027866), 2.5 µl Tagment DNA enzyme I (Illumina 15027865) ...
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bioRxiv - Molecular Biology 2022Quote: ... and subsequently amplified by PCR using KAPA HiFi Hotstart Ready Mix and Nextera Index Kit (Illumina) primers ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were then resuspended in 50 μl transposase reaction mix (1x Tagment DNA buffer (Illumina, 15027866), 2.5 μl Tagment DNA enzyme I (Illumina ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were resuspended in 50 μL of tagmentation reaction mix as per manufacturer instructions (TDE1, Illumina) and incubated at 37°C for 30 mins with gentle agitation every 5 min ...
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bioRxiv - Immunology 2023Quote: ... 3mM MgCl2) on ice and resuspended in 10μl of transposition mix (1X TD Buffer from Illumina, Transposase from Illumina ...
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bioRxiv - Genetics 2023Quote: ... nucleus tagmentation was performed in Tn5 transposase reaction mix (Illumina Tagment DNA Enzyme and Buffer kits) under incubation at 37 °C for 30 min in a thermomixer with shaking at 1,000 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... and nuclei were resuspended in 200μl transposition mix (1x TD buffer containing X µl of Illumina Tagment DNA TDE1 Enzyme per every X nuclei) ...
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bioRxiv - Genomics 2024Quote: ... samples were incubated in cleavage mix (MiSeq Nano kit v2 reagent 4) (Illumina MS-103-1003) for 6 min at 60 °C ...
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bioRxiv - Genomics 2024Quote: ... samples were incubated in incorporation mix (MiSeq Nano kit v2 reagent 1) (Illumina MS-103-1003) for 5 minutes at 60 °C on a flat-top thermal cycler ...
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bioRxiv - Immunology 2022Quote: ... PolyA-enriched strand-specific libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kits (Illumina). All samples were pooled together and 150bp PE reads were sequenced on a Novaseq system ...
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bioRxiv - Microbiology 2021Quote: ... Library preparation was carried out using NEBNext® Ultra II Library Prep kit (Illumina, cat. no. E7645L) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we constructed DNA libraries from 16 samples using an NEBNext Ultra II DNA Library Prep Kit (Illumina). The 15 libraries from branch tissues were sequenced on an Illumina HiSeqX Ten ...
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bioRxiv - Developmental Biology 2023Quote: ... and used for library preparation (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®). Specifically ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA libraries were prepared from 1µg DNA using the NEBNext Ultra II DNA Library Prep Kit (Illumina) following manufacturer’s instructions and sequencing was performed by Novogene (Cambridge ...
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bioRxiv - Plant Biology 2024Quote: ... the RNA was processed with the NEBNext Ultra II RNA Library Prep Kit from Illumina (NEB; E7770L). The libraries were then sequenced on an Illumina NextSeq1000 system and analyzed on the Galaxy platform (59) ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using NEBNext DNA Ultra II library prep kit and sequenced by Illumina MiSeq.
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bioRxiv - Molecular Biology 2021Quote: ... Transposition reactions were performed in 25 µl tagmentation mix (1.25 µl Illumina Tagment DNA Enzyme (Cat# 20034197), 12.5 µl 2X Illumina Tagment DNA buffer ...
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bioRxiv - Microbiology 2020Quote: ... Ribosomal RNA (rRNA) was depleted from 1μg total RNA by addition of rRNA Removal Mix – Gold (Illumina) followed by incubation at 68C for 5min ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was aspirated, and pellets were resuspended in transposase reaction mix (25μL 2xTD Buffer, 2.5μL transposase (Illumina), and 22.5μL nuclease free water) ...
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bioRxiv - Genomics 2021Quote: ... 0.1% IGEPAL CA-630) and 2.5 μL DNA Tagmentation Enzyme mix (Nextera DNA Library Preparation Kit, Illumina) was added ...
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bioRxiv - Immunology 2023Quote: ... 50.000 nuclei per sample were centrifuged at 1000rcf for 5 minutes at 4°C and resuspended in transposition mix (12.5µl TD buffer, 5µl TD enzyme and 7.5µl H2O; Illumina, Catalog # 20034197). Nuclei were incubated at 37°C for 45 minutes and 800rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were resuspended in 50 µl transposition reaction mix with Nextera Tn5 Transposase (Illumina, FC-121-1030) and incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoprecipitated chromatin was used for NGS library preparation (NEBNext Ultra II DNA Library Prep Kit for Illumina, NEB). Libraries were sequenced at the Max Planck Institute of Immunology and Epigenetics using HiSeq 3000 (Illumina ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sheared products were prepared using the New England Biolabs NEBNext Ultra II Prep Kit and sequenced by Illumina MiSeq.
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bioRxiv - Microbiology 2019Quote: ... Each sample was run in three lanes of an Illumina Genome Analyzer II (Illumina, San Diego, California, USA) in a 36 cycle paired end run ...
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bioRxiv - Plant Biology 2022Quote: ... Library construction and sequencing were performed by Novogene using NEBNext Ultra II RNA library prep by Illumina (E7770) and an Illumina Novaseq 6000 S4 instrument ...
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was transposed in 50 μl of the following reaction mix: 1x tagmentation buffer (Illumina, 20034197), 1x PBS ...
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bioRxiv - Immunology 2019Quote: ... The pellet was resuspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl transposase (Illumina) and 22.5 μl nuclease-free water ...
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bioRxiv - Genetics 2020Quote: ... Plates were immediately transferred to ice post-incubation and 12 μL of PCR mix (7.5 μL NPM [Illumina Inc ...
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bioRxiv - Microbiology 2020Quote: Five million fungal spheroblasts or 0.5 ng naked gDNA were resuspended in the tagmentation reaction mix (12.50 μL Nextera 2xTD buffer, 2.00 μL Nextera TDE1 [all Illumina] ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were then resuspended in transposase reaction mix for 30 min at 37 °C (Illumina, Fc-121-1030). The samples were purified using Qiagen MiniElute kit (QIAGEN ...
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bioRxiv - Systems Biology 2022Quote: ... were lysed and transposed simultaneously in 25 µl of transposition mix (0.1% NP40, 0.1% Tween-20, 0.01% digitonin and 5% Tn5 enzyme (Illumina # 15027865) in Tagment DNA Buffer (FC-121–1030 ...
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bioRxiv - Molecular Biology 2022Quote: ... We used Stringtie version 2.1.7 in mix mode (Kovaka et al., 2019) to generate de novo transcriptome assemblies from Illumina RNAseq replicates and pooled Nanopore DRS data ...
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina] ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated nuclei were mixed with 50 µl of transposition reaction mix containing Tagment DNA Enzyme 1 (Illumina, 20034197). Samples were incubated using a thermo shaker at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The pellet was then resuspended in the transposition reaction mix (25 μL 2× TD buffer, 2.5μLTn5 Transposes (Illumina) and 22.5μL nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: ... and pellets gently resuspended in 50 μL of transposition reaction mix (25 μL tagment DNA buffer (Nextera, Illumina), 2.5 μL tagment DNA enzyme (Nextera ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants were carefully removed and the pellets were re-suspended in 25 μL of transposase reaction mix (12.5 μL 2×TD buffer, 1.25 μL TN5 transposase (Illumina) and 11.25 μL nuclease-free water) ...
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bioRxiv - Synthetic Biology 2023Quote: Tagmentation Mix was prepared by mixing 1.25 μL Tagment DNA Buffer and 0.25 μL Tagment DNA Enzyme (Illumina), then added to 1 μL of plasmid DNA at a concentration of 1 ng/μL for a total reaction volume of 2.5 μL ...
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bioRxiv - Cell Biology 2023Quote: ... The nuclear pellet was resuspended in transposition reaction mix using the Tagment DNA Enzyme and Buffer Kit (Illumina) as per manufacturer protocol ...