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Citations for Illumina :
51 - 100 of 418 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... 50’000 nuclei were then pelleted at 500 RCF for 10 min at 4°C and resuspended in 50 pL transposition reaction mix containing 25 pL Nextera 2x TD buffer and 2.5 pL TDE1 (Nextera Tn5 Transposase; Illumina) (cat ...
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bioRxiv - Genetics 2020Quote: ... The cell pellet was resuspended with 50 μl transposition mix containing 25 μl 2X TD buffer (Illumina FC-121-1030), 3.5 μl Tn5 transposase (Illumina FC-121-1030) ...
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bioRxiv - Cancer Biology 2022Quote: ... The nucleic were further resuspended in transposition mix (2X TD buffer, 1X PBS, Digitonin 0.01%, tween 20 0.1%, NFW 5ul, and Illumina transposase 2.5ul). Mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant was removed and the pellet was resuspended in 50 μl of transposition reaction mix (25 μl 2x TD buffer (Nextera DNA Library Prep Kit, Illumina), 2.5 μl TDE1 enzyme (Nextera DNA Library Prep Kit ...
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bioRxiv - Immunology 2020Quote: Permeabilized cells were pelleted for 10 minutes at 500xg and resuspended in 50 μL of transposition mix (25 μL 2x TD buffer (Illumina), 16.5 μL 1X PBS ...
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bioRxiv - Genomics 2021Quote: ... Nuclei were then collected by centrifuging at 500 x g for 10 min at 4°C and nuclei were resuspended in 50 μL of transposition reaction mix [25 μL 2X TD buffer (Illumina), 16.5 μL PBS ...
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bioRxiv - Genetics 2023Quote: ... The nuclei was resuspended in the transposition reaction mix containing 25 μL 2x TD Buffer (Illumina, cat #FC-121-1030), 2.5 μL Tn5 Transposase (Illumina ...
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bioRxiv - Genomics 2022Quote: ... We centrifuged at 500 x g for 10 minutes at 4°C and resuspended cells in the transposition reaction mix (25 µL 2X TD buffer (Illumina), 2.5 µL TDE1 Tn5 transposase (Illumina) ...
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bioRxiv - Cancer Biology 2023Quote: ... nuclei pelleted in ATAC-wash-resuspension buffer and resuspended in transposition mix (2X TD buffer, 1X PBS, Digitonin 0.01%, tween 20 0.1%, NFW 5ul, and Illumina transposase 2.5ul). Libraries were PCR-amplified using the NEBNext Hi-Fidelity PCR Master Mix and Integrated DNA Technologies (IDT ...
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bioRxiv - Neuroscience 2021Quote: ... Then the tagmented DNA was indexed and amplified with 3.75 µL of PCR master mix (Illumina) and 1.25 µL each of i5 and i7 indexing primers (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Add 30 μl PCR master mix (2.5 μl Illumina dual indexes primer 1, 2.5 μl Illumina dual index primer 2 ...
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bioRxiv - Genomics 2022Quote: ... 10 units of T4 PNK (NEBNext® DNA Library Prep Master Mix Set for Illumina, #E6040L) were added to the blunt-ended DNA in NEBNext End Repair Reaction Buffer (NEB ...
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bioRxiv - Microbiology 2023Quote: ... v3 2x 300bp (Illumina). Demultiplexing was performed with the python package demultiplex (Laros JFJ ...
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bioRxiv - Genomics 2020Quote: ... the nuclei were resuspended in the transposase reaction mix (25 μL 2x TD buffer, 2.5 μL Transposase TDE1 (Nextera Tn5 Transposase, Illumina, Cambridge, UK) in a total volume of 50 μL and incubated for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated nuclei were resuspended in 50 μL of Transposition mix (Illumina Tagment DNA Enzyme 100 nM, TD-Buffer 2X (kit from Illumina, 20034197), PBS 0.33% ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was discarded and the pellet (cell nuclei) was resuspended in 50 uL transposition mix (50% v/v 2X Tagment DNA (TD) Buffer (Illumina, Cambridge, UK), 32% v/v PBS ...
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bioRxiv - Immunology 2020Quote: ... The cell pellets were resuspended in 50 μl transposition mix (25 μl 2X TD buffer, 2.5 μl transposase (Illumina, FC-121-1030), 16.5 μl PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell pellet was resuspended in 50 μl transposition mix (25 μl 2X TD buffer, 2.5 μl transposase (Illumina, FC-121-1030), 16.5 μl PBS ...
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bioRxiv - Genomics 2019Quote: ... Nuclei were resuspended in the Nextera transposition reaction mix (25 ul 2x TD Buffer, 2.5 uL Nextera Tn5 transposase (Illumina Cat #FC-121-1030), and 22.5 ul nuclease free H2O ...
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bioRxiv - Genetics 2021Quote: ... The nuclei were resuspended on ice in the transposition reaction mix (2x TD Buffer, 2.5μL Tn5 Transposes and Nuclease Free H2O) (Illumina Cat #FC-121-1030, Nextera) on ice and the transposition reaction was incubated at 37°C for 45 min ...
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bioRxiv - Genomics 2023Quote: ... about 1.5 ng purified pre-amplified cDNA sample was fragmented in four 20 μl tagmentation mix (10 μl 2x TD buffer, 5 μl Nextera XT (Illumina, FC-131-1096), and 5 μl cDNA sample ...
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bioRxiv - Immunology 2023Quote: ... Nuclei were resuspended in the Nextera transposition reaction mix (25 μL 2x TD Buffer, 2.5 μL Nextera Tn5 transposase (Illumina Cat #FC-121-1030), and 22.5 μL nuclease free H2O ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were then transferred on to the ice and the components (15 μl Blunt ligase master mix, 2.5 μl NEB Next adaptor for Illumina, and 1 μl ligation enhancer to a total volume of 83.5 μl ...
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bioRxiv - Cell Biology 2022Quote: ... 10μl of the tagmented chromatin was mixed with 2.5μl of Nextera PCR primer cocktail and 7.5μl of Nextera PCR master-mix (Illumina FC-121-1030) in low-binding PCR tubes ...
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bioRxiv - Microbiology 2020Quote: ... Tagmented DNA fragments were enriched by 10 cycles of PCR amplification using PCR master mix and primers with the index from Illumina. Libraries were quantified by the KAPA SYBR fast quantitative PCR kit (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... we performed single-nucleus indexing by dispensing 600 nL of Nextera PCR Master Mix (NPM) and 400 nL of a unique Nextera index pair (Illumina, cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... and sequenced 2x×150bp by MiSeq (Illumina). A quarter of the MiSeq run (1.2Gb ...
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bioRxiv - Genetics 2019Quote: ... The libraries were amplified using the NPM mix (Nextera PCR Master Mix from Nextera DNA Library Prep Kit) and Index adapters i7 and i5 (Nextera Index Kit, Illumina, U.S), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Second-strand cDNA synthesis was used to assemble a library and sequenced following the manufacturer’s instructions (NEB Next DNA Library Prep Master Mix Set for Illumina; NEB E6040L). Sequenced reads were aligned to the human reference genome hg19 using TopHat v2.1.0 with the ‘--no-coverage-search --read-realign-edit-dist 0’ option selected (17) ...
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bioRxiv - Genomics 2022Quote: ... all of the ChIP DNA and 220 ng of input DNA were mixed with 3 units of T4 DNA polymerase (NEBNext® DNA Library Prep Master Mix Set for Illumina, #E6040L) to create blunt ends ...
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bioRxiv - Microbiology 2020Quote: ... to generate 2x 150bp paired-end reads or 2x 300 bp reads on an Illumina MiSeq (Illumina, San Diego, CA, USA). De novo assembly was also performed identical to the method as described in Coolen et al ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were resuspended in 50 μl of the following PCR master mix with indexed primers from the Nextera DNA Sample Preparation Index Kit (Illumina, FC-121-1011): Phusion HF 2X (NEB ...
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bioRxiv - Genomics 2019Quote: ... the nuclei pellets were resuspended in transposase Master Mix (1.25 μl 10x TD buffer, 5 μl H2O and 6.5 μl of Tn5: Illumina Nextera Kit; FC-121-1031) and incubated for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was conducted using AceQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China) on an RT-PCR system (Illumina Eco, California, USA). The primers used are listed in Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... Sequencing were performed on the Illumina HiSeq 4000 paired end at 2x 100 bp (2x at least 40 million reads) using the TruSeq SBS Kit v4-HS (Illumina, Inc, California, USA).
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bioRxiv - Neuroscience 2022Quote: ... Transcriptomic profile of individual BAT samples was performed using commercial RNA-sequencing kits (NEBNext mRNA Library Prep Master Mix and NEBNext Multiplex Oligos for Illumina, New England Biolabs, Ipswich, MA) and adapted according to previous descriptions [22] ...
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bioRxiv - Genomics 2021Quote: ... and treated with Tn5 transposase in 2x tagmentation buffer (Illumina) for 30 min at 37 C ...
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bioRxiv - Neuroscience 2022Quote: ... Sequencing of 2x 100 bp was done on NovaSeq6000 (Illumina) with a sequencing depth of 50 Mio reads per sample.
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bioRxiv - Genetics 2020Quote: ... 10 μL 2x tagmentation buffer (Illumina, Cat. FC-121-1031), and 1 μL of 8 μM loaded indexed transposase was added per well (See Picelli et al ...
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in 2x tagmentation buffer (Illumina kit) and incubated at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by paired-end (2x 150bp) RNA-seq by Illumina HiSeq 2500 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA libraries were sequenced using a HiSeq 2X platform (Illumina) to generate 150bp pair-end reads ...
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bioRxiv - Cell Biology 2020Quote: ... Fragment Mix (Illumina). End repair ...
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bioRxiv - Molecular Biology 2020Quote: ... finish mix’ (Illumina). Sequencing libraries were prepared using Truseq stranded RNA LT kit (Illumina ...
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bioRxiv - Cell Biology 2020Quote: ... Genome Analyzer II (Illumina) was used to perform 2×36 cycles of paired-end sequencing ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fragment Mix (EPF, Illumina). End repair ...
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bioRxiv - Physiology 2021Quote: ... Fragment High Mix (Illumina) and then subjected to first strand cDNA synthesis with 1 µl Superscript III reverse transcriptase (Life Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... we performed 2x 76-bp paired-end sequencing on a NextSeq500 (Illumina). After quality control using FastQC (Braham Institute) ...
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bioRxiv - Microbiology 2019Quote: ... 2x 8 nt dual indexed adapters from TruSeq RNA CD index kit (Illumina) were added to the libraries for multiplexing ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were then resuspended in transposition buffer (25 μl 2x TD buffer (Illumina), 2.5 μl TDEI (Tagment DNA Enzyme ...