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Citations for Illumina :
151 - 200 of 418 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Libraries were pooled equimolar to a final concentration of 4nM and 1.25 pM sample was sequenced in paired-end 2x 75 bp mode on a NextSeq 500 (Illumina) using a High-Output chip.
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was discarded and pellets resuspended in 20 μl ATAC reaction buffer containing 10 μl 2x transposase buffer and 2.5 μl Tn5 enzyme (Illumina). Samples were incubated at 37 °C for 30 min ...
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bioRxiv - Genomics 2022Quote: ... Supernatant was removed and transposition reaction was set up with 25 μl TD (2x reaction buffer, Nextera Kit, Illumina), 2.5 μl TDE1 (Tn5 transposase ...
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bioRxiv - Genomics 2020Quote: ... The supernatant was discarded and cells were resuspended directly in Dig-transposition buffer (25 μl 2x TD Buffer [Illumina] ...
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bioRxiv - Molecular Biology 2023Quote: ... 15000 cells/sample for mESC were treated with Tagment DNA Buffer 2x reaction buffer with Tagment DNA Enzyme (Illumina) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Pellets were resuspended in 50 µL of transposition reaction (2X TD buffer, 1X PBS, 0.1% Tween-20, 0.1% Digitonin, 5µL of Illumina Tn5 transposase) and incubated for 30 min at 37°C with 1,000 rpm agitation ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared according to manufacturer’s protocol (10x Genomics) and sequenced on a NovaSeq 6000 System using the S4 2x 150 kit (Illumina). Raw reads were aligned to the human transcriptome using a splice-aware algorithm to produce cell-by-gene count matrices ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sequencing was performed on an Illumina Genome Analyzer II (Illumina). Three samples were multiplexed in a lane for a total of 6 lanes ...
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bioRxiv - Microbiology 2021Quote: ... NEBNext Ultra II Directional RNA Library Prep kit from Illumina was used to prepare samples for sequencing ...
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bioRxiv - Microbiology 2021Quote: ... NEBNext Ultra II Directional RNA Library Prep kit from Illumina was used to prepare samples for sequencing ...
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bioRxiv - Genomics 2024Quote: ... and (ii) Illumina NextSeq 500 platform (Illumina, San Diego, CA), at the Quadram Institute ...
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1 ng amplicon DNA was combined with a mix containing 1 μl of Amplicon Tagment Mix (Illumina, FC- 131-1096) and 5 μl of Tagment DNA Buffer (Illumina ...
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bioRxiv - Genetics 2021Quote: ... loaded on an Illumina MiSeq sequencing machine and sequenced for 2x 300 bp paired-end reads with a MiSeq reagent kit V3 (Illumina) to ca ...
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bioRxiv - Molecular Biology 2020Quote: ... and equimolar amounts of library were pooled and sequenced paired-end 2x 75 bp in two sequence runs on a NextSeq 500 system (Illumina).
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was then carefully aspirated and the nuclei were resuspended in 50 μl of transposition buffer (25 μl 2x TD buffer (Illumina), 2.5 μl transposase (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... the two plasmid DNA mixtures were sequenced using an Illumina MiSeq instrument with reagent kit version 3 (2x 300 cycles) as recommended by the manufacturer (Illumina)) ...
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bioRxiv - Cancer Biology 2022Quote: ... whole-genome and targeted libraries were added to flow cell in a 67%-33% ratio and sequenced to approximately 16M fragments per sample corresponding to ~1-2x whole genome coverage using NovaSeq S4 sequencing (Illumina) with 150 bp paired-end reads.
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bioRxiv - Cancer Biology 2022Quote: Cells were incubated with Nextera Tn5 Transposase and 2x TD reaction buffer of the Illumina Tagment DNA Enzyme and Buffer Small Kit (Illumina) for 30 minutes at 37°C ...
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bioRxiv - Genomics 2022Quote: ... The qualified libraries were then quantified by qPCR and sequenced by 2x 150 bp paired-end run on a Novaseq 6000 System (Illumina).
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bioRxiv - Microbiology 2023Quote: ... oligo-dT generated sequencing libraries were prepared at Core Facility at the University of Pittsburgh and 2x 66 bp reads were sequenced on a NextSeq 200 (Illumina). Read libraries were mapped to the human (hg38 ...
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bioRxiv - Genomics 2024Quote: ... was used for a tagmentation reaction that included 3 µL of 2X TMP buffer and 0.5 µL of Transposome (BLT; CAT # 20015880, Illumina Inc.), incubated in a thermocycler at 53 ℃ for 30 minutes with the lid set at 80 ℃ ...
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bioRxiv - Physiology 2024Quote: ... Pellets (nuclei fractions) were resuspended in transposition buffer (50% v/v 2X Tagment DNA (TD) Buffer (Illumina, 336 Cambridge, UK), 32% v/v PBS ...
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bioRxiv - Genetics 2019Quote: ... Cells were incubated in Tagmentation mix (Tagmentation DNA buffer Illumina 15027866 ...
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bioRxiv - Molecular Biology 2022Quote: ... by adding 4 μl of 1× CircLigase II Buffer (Epicentre/Illumina), 2 μl of 50mM of MnCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... we dispensed 600 nL of tagmentation reaction mix containing Tagmentation DNA buffer (TD) and Amplicon Tagment Mix (ATM) at 2:1 v/v ratio (Nextera kit, Illumina, cat. no. FC-131-1096) into each well and performed tagmentation at 55° C for 5 min followed by hold at 4° C in a PCR thermocycler ...
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bioRxiv - Genomics 2019Quote: ... Extracted DNA from the individual male and female was sheared and used for individual libraries followed by 2x 100bp paired-end Illumina sequencing (Illumina, Inc).
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bioRxiv - Evolutionary Biology 2020Quote: ... Two different paired-end sequencing runs (2x 250 bp) were performed on an Illumina MiSeq System (Illumina Inc., San Diego, USA). The obtained raw reads were processed using the pipeline HybPhyloMaker (all scripts available at ...
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bioRxiv - Microbiology 2021Quote: Short-read paired-end sequencing of DNA and rRNA-depleted samples was performed on the Illumina Novaseq platform (S prime, 2x 150 bp) using TruSeq PCR free DNA library preparation kit (Illumina Inc.).
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bioRxiv - Developmental Biology 2022Quote: ... Then the cells were precipitated and kept on ice and subsequently resuspended in 25μl 2X Tagment DNA Buffer (Illumina Nextera kit), 2.5μl Transposase enzyme (Illumina Nextera kit ...
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bioRxiv - Molecular Biology 2023Quote: ... and the nuclei resuspended in 47.5μl of Tagmentation buffer (containing 25μl of 2x Tagmentation buffer from Illumina and 22.5μl of water) before adding 2.5μl of Tn5 enzyme (Illumina Nextera kit ...
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bioRxiv - Microbiology 2019Quote: ... The PCR samples contained 11.2 μL of KAPA polymerase mix (Illumina), 4.4 μL each of the 5 μM column and row indexing primers ...
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bioRxiv - Microbiology 2023Quote: ... index primer mix (IDT for Illumina DNA/RNA UD Indexes, Tagmentation), purified initial PCR product as template (PCR program ...
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bioRxiv - Cancer Biology 2024Quote: ... Isolated nuclei were resuspended in 50 μL of Transposition mix (Illumina Tagment DNA Enzyme 100 nM ...
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bioRxiv - Bioengineering 2021Quote: ... and library preparation (NEBNext Ultra II RNA library prep kits for Illumina). Differential gene expression was tested for each exposure settings against the negative controls that were subject to the same ambient conditions and the same library preparation protocols but with no laser excitation ...
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bioRxiv - Molecular Biology 2020Quote: ... and finally NEBNext Ultra II Directional DNA library preparation kit (Illumina, E75530S) per manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... and (ii) paired-end reads of 320bp fragments with HiSeq2000 platform (Illumina). C ...
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bioRxiv - Cancer Biology 2023Quote: ... and NEBNext® Ultra™ II Directional RNA Library Prep Kit (Illumina), per manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... after adaptor ligation (NEBNext Ultra II DNA Library Prep Kit for Illumina). After sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II kit (Illumina, E7645), followed by paired-end sequencing on a Nextseq2000 using the NextSeq 2000 P2 Reagents 100 Cycles v3 (Illumina ...
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bioRxiv - Genetics 2021Quote: ... 50,000 nuclei were incubated in the transposition reaction mix (20μl nuclease-free water; 25μl 2X Tagment DNA Buffer, Illumina Cat#FC-121-1030; 5μl Tn5 Transposase, Illumina Cat#FC-121-1030) at 37°C for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... Libraries were then pooled to a final concentration of 1.75 nM and sample was sequenced in paired-end 2x 100 bp mode on a NovaSeq 6000 (Illumina, San Diego, sUS) using a S1 chip ...
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bioRxiv - Plant Biology 2020Quote: ... The library pool was sequenced 2x 70 nt paired-end using rapid run mode on Illuminàs HiSeq 1500 50% (Illumina, San Diego, California). Paired end reads of triplicated Wt and hit1 samples were mapped onto the transcriptome v5.5 of Chlamydomonas reinhardtii with kallisto (Bray et al. ...
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bioRxiv - Cell Biology 2021Quote: ... A one-step gentle membrane permeabilization and DNA transposition was performed by adding 50ul transposition mixture (25 uL TD buffer 2X, 2,5uL of transposase TDE1 (Illumina, San Diego, California), 0,5 uL digitonin 0,1% (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The libraries were sequenced using an Illumina MiSeq with MiSeq Reagent Kit v.3 (2x 300 bp, Illumina, San Diego, CA, USA) according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... The nuclei pellet was then immediately resuspended in 25 μ L of 2x tagmentation buffer containing 2 μ L of TDE1 (Illumina). The reaction was placed at 37C for 30 minutes with gentle mixing three times ...
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bioRxiv - Genomics 2019Quote: ... The pellet was resuspended in the transposase reaction mix (Nextera kit, Illumina). The transposition reaction was performed at 37°C for 30 min and was followed by purification of the samples using the Qiagen MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... Fresh nuclei were treated with Transposition mix and Purification (Illumina #FC121-130), the nuclei were incubated at 37 °C in Transposition Reaction Mix (25 μL reaction buffer ...
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bioRxiv - Genomics 2021Quote: ... they were resuspended in 50 μl Transposase Mix (1x TD buffer (Illumina), 100 nM Nextera Tn5 Transposase (Illumina) ...
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bioRxiv - Genetics 2021Quote: ... The pellet was resuspended in transposition reaction mix (Nextera Tn5 transposase, Illumina) and incubated for 30 min at 37°C ...
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bioRxiv - Genomics 2022Quote: ... Pelleted nuclei were incubated in 50 μl transposition reaction mix (20034210, Illumina) at 370C for 30 minutes ...