Labshake search
Citations for Illumina :
301 - 350 of 418 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit from Illumina (New England Biolabs #37645) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... paired-end (2 × 150bp) RNA libraries were prepared following standard protocols for NEBNext Ultra II RNA Library Prep (Illumina) and sequenced on the Novaseq6000 platform at Novogene Co ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA libraries were prepared with the NEBNext Ultra II RNA-Seq kit and sequenced on a NextSeq 500 (Illumina) system using 75bp single reads ...
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bioRxiv - Cell Biology 2022Quote: ... 600 ng RNA was used for library preparation using the NEBNext Ultra8482 II Directional RNA Library Prep Kit (Illumina) and the QIAseq FastSelect RNA Removal Kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... Genotyping was carried out using the Infinium II HumanHap 550K Genotyping BeadChip version 3 (Illumina, San Diego, California USA). Collection and purification of DNA have been described previously (Kayser et al. ...
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bioRxiv - Neuroscience 2019Quote: ... The amplified cDNA underwent library preparation using the NEBNext Ultra II DNALibrar kit and subject to sequencing by Illumina Hiseq 2500 platform ...
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bioRxiv - Genomics 2021Quote: ... and sequencing was performed using eight SMRT Cells on a PacBio Sequel II instrument (Illumina, San Diego, California, USA). This yielded 787.76 Gb of sequence data ...
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bioRxiv - Genomics 2023Quote: ... and sequenced by PacBio Sequel II. Hi-C libraries were prepared following a standard protocol (Belton et al. 2012) and sequenced by Illumina HiSeq 4000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: Library preparation was performed according to manufacturer’s instructions (NEBNext® Ultra™ II DNA Library Prep Kit for Illumina) using the following modified library amplification program ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB). The size of the resulting library was controlled by use of a D1000 ScreenTape (Agilent 2200 TapeStation ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was used for library preparation with NEBNext Ultra-low input II Directional RNA Library Prep Kit (Illumina) and sequenced on an Illumina HiSeq with 2×150 bp sequencing configuration (Illumina).
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bioRxiv - Molecular Biology 2024Quote: ... and RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit from Illumina (NEB, E7770) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were extracted by resuspending the samples in 50 μl transposition reaction Tn5 mix (Illumina Nextera DNA Library Kit) (FC121-1030 ...
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bioRxiv - Developmental Biology 2021Quote: ... The nuclei pellet was then resuspended in the 50ul of the transposition reaction mix (25ul Tagment DNA buffer, 2.5ul TD Tn5 Transposase enzyme, 22.5ul nuclease-free water) (Illumina; 20034197). The transposition reaction was incubated at 37°C for 30 min on a thermomixer with constant gentle shaking at 1000 rpm ...
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bioRxiv - Developmental Biology 2020Quote: ... Next, the pellet was resuspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl transposase (Illumina) and 22.5 μl nuclease-free water) ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were re-suspended in transposase reaction mix (25 μL 2×TD buffer, 2.5 μL transposase (FC-121-1030, Illumina) and 22.5 μL nuclease-free water ...
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bioRxiv - Cell Biology 2022Quote: ... the pellet was resuspended and incubated in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl transposase (Illumina), and 22.5 μl nuclease-free water) ...
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bioRxiv - Genomics 2022Quote: ... The pellet was resuspended in 25 μL of ATAC-seq transposition mix (12.5 μL 2× Illumina Tagment DNA (TD) buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Next, the pellet was resuspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl transposase (Illumina) and 22.5 μl nuclease-free water) ...
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bioRxiv - Immunology 2022Quote: ... The nuclear pellet was gently resuspended in a final volume of 50 μl transposase reaction mix (25 μL 2 × TD buffer, 2.5 μL Illumina Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina ...
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bioRxiv - Immunology 2022Quote: ... The nuclear pellet was gently resuspended in a final volume of 50 μl transposase reaction mix (25 μL 2 × TD buffer, 2.5 μL Illumina Tn5 transposase (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Next, the pellet was resuspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl transposase (Illumina) and 22.5 μl nuclease-free water) ...
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bioRxiv - Cancer Biology 2019Quote: ... After centrifugation, the pellet was carefully resuspended in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 (Illumina), and 10.25 μl nuclease-free water ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells were re-suspended in the transposase reaction mix (25 μl 2× TD buffer, 2.5 μl Tn5 transposase [in the Nextera DNA Sample Preparation Kit, Illumina, Cat ...
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bioRxiv - Cell Biology 2019Quote: ... Pelleted nuclei were resuspended in 50□μl of transposition mix (25□μl 2□×□TD buffer, 2.5□μl Tagment DNA enzyme (Illumina Nextera DNA Library Preparation Kit ...
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bioRxiv - Genomics 2021Quote: ... Centrifugation at 500 g for 10 minutes at 4°C made a pellet of nuclei, which was resuspended in 50 μL transposition mix (25 μL 2× TD buffer, 2.5 μL transposase (Illumina), 16.5 μL PBS ...
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bioRxiv - Genomics 2021Quote: ... Supernatant was discarded and the pellet including the nuclei was resuspended in a 50 μl transposition reaction mix (Illumina Tagment DNA Enzyme and Buffer Kit ...
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bioRxiv - Genomics 2021Quote: ... The cell pellets were then resuspended in 20 μl, 10 μl, and 8 μl of transposition mix (25 μl 2×TD buffer, 2.5 μl Tn5 (Illumina), 16.5 μl PBS (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... 2.5 µl of Tagment DNA buffer 1.25 µl of Amplicon Tagment Mix (Nextera XT kit, Illumina FC-131-1096) were added ...
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bioRxiv - Neuroscience 2022Quote: ... 0.8 ul of each normalized sample was mixed with 2.4 ul of tagmentation mix containing Tn5 Tagmentation enzyme (20034198, Illumina) and then incubated at 55°C for 12 minutes ...
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bioRxiv - Immunology 2023Quote: ... Pelleted nuclei were resuspended in 50 μl of transposition mix (25 μl 2 × TD buffer, 2.5 μl Tagment DNA enzyme (Illumina Tag DNA Enzyme and Buffer Kit ...
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bioRxiv - Genomics 2023Quote: ... about 40 ng purified pre-amplified Hi-C sample was fragmented in two 50 μl tagmentation mix (1x TD buffer and 0.5 μl TDE1 (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit ...
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bioRxiv - Physiology 2024Quote: ... tagmented DNA was amplified by PCR in a reaction mix (5 µL DNA, 2.5 µL of 25 μM forward primer (Nextera/Illumina i5 adaptors (Illumina)) ...
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bioRxiv - Immunology 2023Quote: ... and the nuclei incubated for 2 hours at 37°C with 50μl of TDE1/TD transposase reaction mix (Illumina). Tagmented DNA was then purified using the MinElute kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Pelleted nuclei were incubated in transposase reaction mix (1 X TD reaction buffer, 2.5 µl Tn5 transposase (Nextera, Illumina)) for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were added with 50mL trans-position reaction mix of Nextera DNA library preparation kit (FC-121-1031, Illumina). DNA was amplified by PCR and incubated at 37°C for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... with 1.0 μL of PCR1 product and 1.2 μL of each barcode primer mix (Access Array Barcode Library for Illumina Sequencers-384 ...
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bioRxiv - Genomics 2023Quote: ... resuspended in 50 μl of transposition reaction mix (25 μl of Tagmentation DNA buffer, 2.5 μl Tagment DNA enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kits ...
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bioRxiv - Genomics 2023Quote: ... tn5-digested ‘input’ controls were made by performing tagmentation for 50 ng iXist-ChrX genomic DNA by a basic 50 μl transposition mix of 1X TDE buffer and 2.5 μl TDE1 Enzyme (Illumina) in H2O ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell pellet was resuspended in transposition reaction mix (12.5 μl TD-Buffer, 1.25 μl Tn5, 11.25 μl water) (Nextera DNA Library Prep Kit, Illumina, Cat# 15028212) and incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... was isolated from pooled E8.0-E8.5 stage embryos using Isogen II reagent (Nippongene) and subjected to RNA-seq library preparation using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) based on manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ChIP-seq and DNase-seq libraries were prepared from 1-5 ng ChIP DNA or DNase DNA samples using NEBNext Ultra II DNA library Prep Kit (Illumina). Libraries were sequenced on an Illumina NextSeq 500 with single-end or paired-end 75 bp reads.
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bioRxiv - Microbiology 2020Quote: ... A reference genome from strain QMA0248 was constructed using both long reads derived from a single Smrt Cell using the PacBio RS II system with P4C2 chemistry and short reads from Illumina HiSeq2000 derived from Nextera XT paired-end libraries as reported elsewhere (NCBI accession no ...
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bioRxiv - Cancer Biology 2020Quote: The RNA-seq libraries (n=3 per experimental group) were prepared using the NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2022Quote: ... MEFs and NPCs were used to prepare NGS libraries based on the NEB Next Ultra II directional RNA library preparation kit from Illumina. As default ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were constructed using NEB polyA RNA ultra II and subsequently subjected to 150 cycles of sequencing on NovaSeq-6000 (Illumina). Adapters were trimmed using Cutadapt (v2.3) ...
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Peripheral neuropathy linked mRNA export factor GANP reshapes gene regulation in human motor neuronsbioRxiv - Neuroscience 2021Quote: The RNA-seq libraries were made with NEBNext Ultra II Directional (PolyA) kit and sequenced with NovaSeq SP 2×150bp (Illumina) at Biomedicum Functional Genomics Unit (FuGU) ...
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bioRxiv - Genetics 2021Quote: ... one 150 bp paired end (350 bp insert size) Illumina library was prepared from the same DNA (NEBNext Ultra II DNA Library Prep Kit for Illumina) and sequenced to approximately 140 fold coverage (24.6 Gb ...
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bioRxiv - Microbiology 2020Quote: ... Libraries were prepared using the NEB Next Ultra II kit (New England Biotech, Ipswich, MA) and paired-end sequenced (2×150bp) on a NextSeq system (Illumina). Adaptors were removed using cutadapt v1.10 [28] and reads assembled using SPAdes v3.7.1 [29] ...
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bioRxiv - Genomics 2021Quote: ... using the TruSeq DNA PCR-free library prep kit with fragments of 150 bp and ii.) Nextera Mate Pair library prep with fragments of 2,000 bp (Illumina, USA). The short-read paired-end libraries were sequenced in two independent lanes and the mate-pair library in one lane using the Illumina HiSeq4000 platform (Illumina ...