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Citations for Bioline :
1001 - 1050 of 1617 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... Converted DNA was subjected to 12 cycles of PCR amplification to enrich for adapter-ligated fragments with MyTaq Mix (Bioline Inc.), during which each sample was also barcoded with unique sequence tags ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions of all DNA samples were performed using the SensiFAST SYBR No-ROX Kit (Bioline) on a Mic (Magnetic Induction Cycler ...
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bioRxiv - Immunology 2021Quote: ... dNTPs (Bioline), FAM (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription of 0.2-0.5 μg total RNA was performed using 50 U Tetro reverse transcriptase (Bioline, London, UK), 40 mM dNTPs (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... using SYBR Green qPCR mix (Bioline) on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplification was performed in 25 µl containing Biomix reaction mix (Bioline) and ultra-stable Taq DNA polymerase (Bioline) ...
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bioRxiv - Molecular Biology 2021Quote: ... and ultra-stable Taq DNA polymerase (Bioline). Amplicons were subjected to electrophoresis through 1% w/v agarose ...
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bioRxiv - Molecular Biology 2021Quote: ... Schistosome cDNAs were reverse transcribed from the total RNAs using the SensiFAST cDNA Synthesis Kit (Bioline) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... after which α-Select Bronze Efficiency Competent Cells (Bioline) were transformed with the ligation products ...
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bioRxiv - Molecular Biology 2021Quote: ... or the ISOLATE II RNA Plant kit (Bioline, Meridian Life Science). RNA integrity was confirmed by either agarose gel electrophoresis or by capillary electrophoresis in an Agilent 2100 Bioanalyzer according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The final PCR product (sequencing library) was purified with JetSeq Beads (Bioline, Cat # BIO-68031), followed by library quality control check ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of 5X Mytaq reaction buffer (Bioline, London, UK), 0.2 μl of MyTaq DNA polymerase (Bioline ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μl of MyTaq DNA polymerase (Bioline, London, UK) and 1 μl of DNA template ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using the SensiFASTTM Probe Lo-ROX One-Step kit (Bioline, UK). Primers and probe sequences ...
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bioRxiv - Immunology 2021Quote: ... Real time quantitative PCR (RT-qPCR) analysis was carried out using the SensiMix SYBR No-Rox kit (Bioline), using primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Immunology 2021Quote: ... qPCR-ChIP was carried out using the SensiMix SYBR No-Rox kit (Bioline), with the primers and conditions shown in Supplemental Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... with Sensi-FAST Probe Hi-ROX qPCR kit (Bioline, 82005) (8).
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bioRxiv - Immunology 2021Quote: ... using the SensiFAST SYBR No-ROX Kit (Bioline, Meridian Bioscience). Hprt1 was used as a housekeeping gene and the standard curve method was used for quantification ...
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bioRxiv - Immunology 2021Quote: ... TRIsure reagent (Bioline, obtained through Thomas Scientific ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was performed using the SensiFASTTM Probe Lo-ROX One-Step kit (Bioline, UK). Primers and probe sequences ...
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bioRxiv - Microbiology 2021Quote: ... 7 μL of SensiMix™ SYBR® Green Master Mix (Bioline®, Memphis, TE, USA), and H20 in a total volume of 15 μL ...
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bioRxiv - Microbiology 2021Quote: ... Libraries of cDNA were created using SensiFAST cDNA Synthesis Kit (Bioline). Control reactions without reverse transcriptase were conducted to confirm the absence of contaminating DNA in all samples ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was performed using the SensiFAST Probe Lo-ROX One-Step kit (Bioline Meridian, Australia) with a reaction volume of 20µl ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed in triplicates using SensiFAST SYBR No-ROX Kit (Bioline) in Rotor-Gene 6000 (Corbett Life Science) ...
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bioRxiv - Cell Biology 2021Quote: ... and all the used strains were genotyped before its use using MyTaqTM DNA polymerase (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The reagents used are TRIsure (Bioline, England), SYBR (Bioline ...
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bioRxiv - Cell Biology 2021Quote: The total RNA was extracted using TRIsure (Bioline, England). Prepared cells were distributed into an 8 pi medium consisting of 1 × 106 cells on 100 pi plates ...
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bioRxiv - Cell Biology 2021Quote: ... and the SensiFAST SYBR Lo-Rox Kit (Bioline, England). The sequences of primers used in the experiment are presented in Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR (Bioline, England), Reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: mRNA was isolated using NucleoSpin® RNA Plus (Machery-Nagel) and 1 µg of total mRNA was reverse-transcribed into cDNA using SensiFASTTM cDNA Synthesis Kit (Bioline) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA (100 ng) was analysed by quantitative PCR using SensiFASTTM SYBR Lo-ROX Kit (Bioline) in the 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Genomics 2021Quote: ... cDNA was synthesized using Bioscript reverse transcriptase (Bioline), Random Primers (Thermo Fisher) ...
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bioRxiv - Genomics 2021Quote: ... qPCR amplification was performed in triplicate with SYBR green qPCR chemistry (Bioline) using primers for Tnfa (F-5’- ACCTCACACTCAGATCATCTTCTC-3’ ...
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bioRxiv - Genomics 2021Quote: ... Amplification was performed in duplicate using SYBR green qPCR chemistry (Bioline) using a universal barcode forward primer (F-5’- GCTTGGTTAGAATGGGTAACTAGTTTGCAG-3’ ...
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bioRxiv - Genomics 2021Quote: ... ans Isolate II Genomic DNA kit (Bioline) was used to extract genomic DNA from 1×107 cells ...
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bioRxiv - Genomics 2021Quote: ... One-step reverse-transcription quantitative PCR (RT-qPCR) was performed with the SensiFAST™ SYBR® No-ROX One-Step kit (Bioline®) on an Eco™ Real-Time PCR System (Ilumina® ...
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bioRxiv - Molecular Biology 2021Quote: ... Each sample was measured in triplicate of 5 μl with the following final concentrations: Velocity Polymerase 0.6 U (Bioline), 1.2 x Hifi Buffer (Bioline) ...
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bioRxiv - Immunology 2021Quote: ... the cDNA was synthesized using SensiFAST reverse transcriptase (Bioline), and quantitative PCR was run using SensiFAST Sybr Master Mix (Bioline) ...
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bioRxiv - Immunology 2021Quote: ... and quantitative PCR was run using SensiFAST Sybr Master Mix (Bioline). The primers used to detect GFP were a1 5′-caagggcgaggagctgttca-3′ and 5′-tgaacttgtggccgtacgtcg-3′ (GFP7) ...
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bioRxiv - Molecular Biology 2021Quote: ... and SensiFAST SYBR Hi-ROX mix (Bioline). Total reaction volume was 10 µl with 150 nM of each primer ...
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bioRxiv - Immunology 2021Quote: ... obtained from the TaqMan RT-PCR kit (Bioline cat# BIO-78005), RT ...
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bioRxiv - Immunology 2021Quote: ... 5µl of 10 mM dNTP mix (Bioline, Catalogue no.-BIO-39044), and 1µl of RNaseOUTTM Recombinant Ribonuclease Inhibitor (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was carried out using the Bioline SensiFASTTM Probe Lo-ROX Kit (Bioline, Catalogue no.-84005) and Stratagene Mx3005P (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-quantitative PCR (RT-qPCR) was performed using a SensiMix SYBR kit (Bioline, #QT605-05) on an Applied Biosystems ViiATM7 Real-Time PCR machine ...
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bioRxiv - Microbiology 2021Quote: ... Total cDNA was prepared by the SensiFAST™ cDNA Synthesis Kit (Bioline, Memphis, USA) and analyzed by next generation sequencing service (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... After reverse-transcription by the SensiFAST™ cDNA Synthesis Kit (Bioline, Memphis, USA), cDNA samples were prepared with the QuantiFast SYBR Green PCR Master Mix reagent (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation at 4000 rpm for 15 minutes and pellets used for RNA extraction by RNeasy kit according to the manufacturer’s protocol (Bioline). cDNA synthesis and RT-qPCR was performed as previously described (60) ...
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bioRxiv - Plant Biology 2020Quote: ... SYBR Green reaction mix (Bioline; Sensimix SYBR No-ROX kit) was used in a Bio-Rad CFX384 real-time system for qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... RNA extraction was carried out using Trisure® (Bioline, Australia) by following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA library construction was performed using SensiFAST™ cDNA Synthesis Kit (Bioline, Australia).