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Citations for Bioline :
1001 - 1050 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: RNA from GP barley infected with Fg-IFA65 at 5 dpi and an uninfected control was extracted with the Isolate II plant miRNA kit (Bioline). 1 µg of RNA (>200 nt ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was used to quantify transcript levels of specific quorum sensing-controlled genes in DS40M4 and was performed using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: Real-time qRT-PCR (quantitative reverse transcription PCR) was used to quantify transcript levels using the SensiFast SYBR Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from the enriched viral nucleocapsids using the Isolate II genomic DNA kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... PCR amplification of DpnII-digested fragments using MyTaq (Bioline, BIO-21112) enriched for methylated fragments before samples were sonicated and prepped for sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... First strand synthesis was performed using Bioscript (Bioline). mRNA expression levels were quantified using SYBR-Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in TriSure (Bioline) and RNA was extracted according to the manufacturers’ protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Bands of correct size were cut out and purified using the Isolate II PCR and gel kit (Bioline). 50 ng of vector and 150 ng of each fragment were mixed with 3 μl of HiFi DNA Master Mix (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... using random primers (Bioline, UK). One µl of cDNA was specifically and quantitatively amplified using Biotool 2x SybrGreen qPCR master mix (Stratech ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted using the Isolate II RNA Mini kit (Bioline, UK). 1-3 µg were reverse transcribed with a MuLV reverse transcriptase (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed using SensiFast SYBR Hi-ROX qPCR kit (BioLine). rp49 was used as a housekeeping gene for ΔΔCt calculations ...
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bioRxiv - Cell Biology 2021Quote: ... and cDNA was synthesised with SensiFast cDNA synthesis kit (Bioline, London, UK) according to the manufacturers’ protocols ...
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bioRxiv - Microbiology 2021Quote: ... After PCR verification in agarose gel and PCR clean-up using the ISOLATE II PCR and Gel Kit (Bioline), the remaining plasmid template was digested with DpnI at 37°C for 3h ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR reactions were set up using SensiFast 2× Mastermix (Bioline Cat# BIO-94020) and oligonucleotides targeting genes of interest (IDT ...
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bioRxiv - Cell Biology 2021Quote: ... 500 ng of total RNA from each sample were reverse transcribed to cDNA using the Bioline SensiFAST cDNA Synthesis kit (Bioline, Cat# BIO-65054). The cDNA was diluted 13-fold in ddH2O and 4 uL of this dilution was used for each quantitative real-time PCR (qPCR) ...
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bioRxiv - Cell Biology 2021Quote: ... or The SensiMix™ SYBR® No-ROX (BioLine) protocols for Real TimeOne-Step RT-PCR using the Real Time Rotor-GeneRG-3000™ light cycler from Corbett Research using primers listed in Supplementary Table IV.
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bioRxiv - Microbiology 2021Quote: ... and 1.25 U BIOTAQ™ DNA Polymerase (Bioline, London, UK). A Mastercycler Nexus GSX1 (Eppendorf ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR reactions were conducted using gene-specific primers with SensiFAST SYBR No-ROX kit (Bioline, #Cat no: BIO-98005) as described previously (Sibai et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... The conversion of messenger RNA into cDNA was performed using the Sensifast cDNA synthesis kit (Bioline, BIO- 65053). cDNA was then amplified by PCR using specific primers for each gene designed with Primer-blast software (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Cell Biology 2021Quote: ... For conventional PCR we used a commercial master mix 2x My Taq HS Mix (Bioline Bio-25046). PCR amplifications were carried out in the T-100 Thermal Cycler (Biorad) ...
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bioRxiv - Cell Biology 2021Quote: ... or The SensiMix™ SYBR® No-ROX (BioLine) protocols for Real TimeOne-Step RT-PCR using the Real Time Rotor-GeneRG-3000™ light cycler from Corbett Research using primers listed in Supplementary Table IV.
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bioRxiv - Plant Biology 2021Quote: ... Each 20 μL reaction consisted of 10 μL of SensiFAST Probe No-ROX Mix (2×) (Bioline, Australia), 0.25 μM of each forward and reverse primer (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2021Quote: ... Each 20 μL reaction consisted of 10 μL of SensiFAST Probe No-ROX Mix (2×) (Bioline), 0.25 μM each of RamF6 and RamR6 primers (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... with the SensiMix Kit and SYBR Green (Bioline, Luckenwalde, Germany). Negative controls without template addition ...
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bioRxiv - Microbiology 2021Quote: ... 2μL were used as template for genomic PCRs in a 50 μL reaction with Velocity DNA Polymerase (Bioline#21098) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the K13 locus was amplified directly from whole blood using the primer pair p26+p27 (Table S9) and the MyTaq™ Blood-PCR Kit (Bioline). Primer pairs p39+p40 and p50+p51 were used to amplify fd and mdr2 ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was synthesized from 1 µg total RNA using cDNA synthesis kit (Bioline, Sydney, Australia) and RT-PCR was performed using the SYBR® Select Master Mix (Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... washed in water and 100% ethanol prior to RNA extraction using the ISOLATE II RNA Mini Kit’s protocol (Bioline, UK). The extracted RNA was quantified using Nanodrop (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR reactions were performed in triplicate using a Corbett Rotorgene 6000 Real-Time PCR machine with Sensimix SYBR No-Rox (Bioline, UK) and intron-spanning primers (Table S1) ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time quantitative reverse transcriptase PCR (RT-qPCR) was performed using a Biorad CFX384 Optics Module with SensiFAST SYBER No-ROX (Bioline Meridian Biosystems) using gene-specific primers listed in Supplementary Table 1 ...
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bioRxiv - Plant Biology 2021Quote: Total RNA extracted from was extracted using TRIsure reagent (Bioline Meridian Biosystems). Total RNA (1-5 μg ...
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bioRxiv - Plant Biology 2021Quote: ... and reverse-transcribed using Tetro Reverse Transcriptase (Bioline Meridian Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted by grinding snap frozen tissue to a fine powder in liquid nitrogen with a motor and pestle using TRIsure reagent (Bioline Meridian Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... purified using TRIsure reagent (Bioline Meridian Biosystems), denatured and annealed prior to use.
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bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed with the SensiFastTM SYBR No-Rox Kit (Bioline) in a Roche Light Cycler 480 as described by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were diluted and 5 μl was used for genotyping PCR using MyTaq DNA polymerase (Bioline) with indicated DNA primers ...
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bioRxiv - Genetics 2021Quote: ... genomic DNA was extracted using the ISOLATE II Genomic DNA kit (Bioline). Following DNA amplification and fragmentation according to the associated Illumina HTS assay protocol samples were hybridized to an Infinium PsychArray v1.1 BeadChip (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: Ear biopsies from 21 days old mice were digested in MyTaq Extract PCR kit according to the manufacturer (Bioline, Cat number BIO-21126). PCR amplification was performed using the high-fidelity enzyme Q5 from New England Biolab (NEB ...
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bioRxiv - Genetics 2021Quote: ... 1 µg of total RNA was reverse transcribed using SensiFAST cDNA Synthesis Kit following the manufacturer’s protocol (Bioline).
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bioRxiv - Genetics 2021Quote: Quantitative polymerase chain reaction (qPCR) was performed using SensiFAST™ SYBR No-ROX Kit (Bioline, BIO-98020) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... purified using PureLink Quick Gel Extraction and PCR Purification Combo Kit” (Bioline) and eventually Sanger sequenced to finally prove their identity and the back-splice junction ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA synthesis was performed using the Tetro cDNA synthesis kit (Bioline). 2x GoTaq Green master mix (Promega ...
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bioRxiv - Microbiology 2021Quote: ... SureClean was applied to purify DNA or vector between steps as described by the manufacturer (Bioline GmbH, Luckenwalde, Germany). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... 5μl SensiFAST SYBR Green No-ROX Kit (BIOLINE, BIO-98020), and 2μl of DNAse/RNAse-free water ...
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bioRxiv - Microbiology 2021Quote: ... Bands of approximately 350 bp were excised by comparing to a 50 bp ladder (Bioline), and subsequently purified using the MinElute Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... using 5 μl product with 1 μl 5X loading dye (BioLine) to verify bands of approximately 450 bp ...
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bioRxiv - Microbiology 2021Quote: For quantification of cellular TMPRSS2 and GAPDH expression cDNA synthesis and qPCR were performed according to the manufacturer’s instructions using the SensiFast cDNA kit and SensiFAST SYBR qPCR kit (both from Bioline). The qPCR was run on a StepOnePlus realtime PCR cycler (Thermo ...
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bioRxiv - Microbiology 2021Quote: ... 125nM) and SensiFAST Probe Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s instructions in a 20μl reaction with 5μl sample ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was carried out using the SensiFAST SYBR Hi-ROX kit (Bioline, BIO92005). Briefly ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated small RNA (bound by AGO4) was released from beads and isolated by TRIsure (Bioline) extraction ...