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Citations for Bioline :
851 - 900 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... qPCR was performed using SensiFast SYBR Hi-ROX kit (Bioline) on CFX96 Touch Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: Genotyping of ClC-7 KO U2OS clone was performed using an Extract PCR-Kit (Bioline) with the following primers ...
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bioRxiv - Cancer Biology 2021Quote: ... and oligo (dT)18 primers (Bioline #BIO-38029). The RT-qPCR reaction was performed using SensiFAST SYBR Hi-ROX Kit (Bioline #BIO-92020 ...
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bioRxiv - Cancer Biology 2021Quote: ... The RT-qPCR reaction was performed using SensiFAST SYBR Hi-ROX Kit (Bioline #BIO-92020) on the StepOnePlus™ platform (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Real-time RT-PCR using SensiFast SYBR No-Rox kit (Bioline) was performed to determine gene expression ...
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bioRxiv - Immunology 2021Quote: ... Sensifast cDNA synthesis kit (Bioline) was used to transcribe total RNA to cDNA ...
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bioRxiv - Immunology 2021Quote: ... using random hexamers (Bioline Reagents Ltd) following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... obtained from the TaqMan RT-PCR kit (Bioline BIO-78005), was added to a 15 ml tube ...
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bioRxiv - Immunology 2021Quote: ... which was conducted using the SensiFAST SYBR No-ROX Kit (Bioline, Singapore) on the QuantStudio 5 or QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was extracted using the isolate II RNA minikit (Bioline). Quantitative reverse transcriptase-PCR (QrtPCR ...
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bioRxiv - Genetics 2021Quote: ... RNA was isolated from tissue samples using Isolate RNA mini kit (Bioline) and cDNA was prepared with Tetro cDNA Synthesis kit (Bioline) ...
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bioRxiv - Genetics 2021Quote: ... PCR products were extracted from the agarose gel with ISOLATE II PCR and Gel Kit (Bioline) and Sanger sequenced.
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bioRxiv - Genetics 2021Quote: ... and cDNA was prepared with Tetro cDNA Synthesis kit (Bioline). qPCR was performed on Applied Biosystems ViiA™ 7 Real-Time PCR System with Sybr Green ...
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bioRxiv - Genetics 2021Quote: Samples used for RFLP analysis were amplified with MyTaq™ Red Mix (Bioline BIO-25043) and subcloned using Strataclone PCR Cloning Kit (Agilent 240205) ...
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bioRxiv - Genetics 2021Quote: ... cDNA was synthesized using the SensiFast cDNA synthesis kit (Bioline, USA), and qPCR was done using the SYBR green mix (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... and then digested with proteinase K (20 μg per sample at 56°C for 2 h; Bioline, cat. # 37084), and electrophoresed on a 1.5% agarose gel to verify that the majority of fragments were 100-400 bp in size ...
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bioRxiv - Genetics 2021Quote: ... RNA was reverse-transcribed (1 µg each experimental point) by using SensiFAST™ cDNA Synthesis Kit (BIO-65053, Bioline) and qPCR was performed using SensiFast Sybr Lo-Rox Mix (BIO-94020 ...
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bioRxiv - Genetics 2021Quote: ... and qPCR was performed using SensiFast Sybr Lo-Rox Mix (BIO-94020, Bioline). The run was performed by using the Applied Biosystems (Waltham ...
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bioRxiv - Developmental Biology 2021Quote: ... For methylated DNA amplification by PCR each one of these products was mixed with 118 μl of DamID PCR buffer and 2 μl (10 units) of MyTaq™ DNA polymerase (Bioline) and were aliquoted at 40 μl in 4 different 0.2 ml PCR tubes ...
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bioRxiv - Developmental Biology 2021Quote: ... The DamID PCR buffer consisted of 1.36x MyTaq™ Buffer (Bioline) and 1.06 μM of the DamID PCR primer (Adr_PCR ...
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bioRxiv - Genetics 2021Quote: ... A total of 14 PCR cycles were performed using MyTaq Red Mix (#BIO-25043; Bioline), yielding ~30 μg barcodes ...
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bioRxiv - Genetics 2021Quote: ... qPCR was conducted with SensiMix SYBR Green (Bioline) on the StepOne Plus (Life Technologies ...
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bioRxiv - Genetics 2021Quote: ... target genomic regions were amplified by PCR with BioMix Red (Bioline), sent for Sanger sequencing by MCLAB (South San Francisco ...
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bioRxiv - Genetics 2021Quote: ... resuspended in Trisure (#BIO-38032; Bioline) and frozen at −80 °C until further processing ...
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bioRxiv - Genetics 2021Quote: ... cDNA was synthesized using the SensiFast cDNA synthesis kit (Bioline, USA), and qPCR was done using the SYBR green mix (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The double -stranded cDNA was purified using JetSeq Beads (Bioline, Cat # BIO-68031). The purified cDNA was end-repaired ...
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bioRxiv - Microbiology 2021Quote: ... using SensiFast probe No-ROX mastermix (Bioline, London, United Kingdom) and with programme described in Table S2 ...
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bioRxiv - Molecular Biology 2021Quote: ... For the semi-quantitative end-point PCRs the MyTaq Red Mix (Bioline) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... were amplified using a MyTaq™ (Bioline, U.K.) reaction mixture and thermal profile as previously described by Harper et al32 ...
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bioRxiv - Molecular Biology 2021Quote: ... Real-time PCR analysis was carried out using SYBR Green mix (Bioline) in a Bio-Rad CFX-100 RT-qPCR ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR reactions were performed in triplicate on 5ng of cDNA using SensiFAST SYBR No-ROX (Bioline #BIO-98005) on a LightCycler 96 (Roche Life Science) ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR was done using gene-specific primers (see Supplemental Table 1) in technical triplicates on a LightCycler 480 system using the Sensifast SYBR Master mix (Bioline). The ratio of experimental target mRNA to an ACTIN control for each sample was calculated by Applied Biosystems software ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was made from 1 ug total RNA using Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline). cDNA was diluted into 100 ul and 2 ul of cDNA was used to perform qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: ... Each reaction (20 μL) consisted of 1 μL of cDNA substrate and 19 μL of a SensiFAST No-ROX Kit Master Mix (Bioline, UK). Following primer optimisation ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesised from 50 ng RNA using a SensiFAST cDNA Synthesis Kit (Bioline), with a combination of random hexamers and oligo dT primers ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was performed on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline) with the following PCR cycle ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... First-strand cDNA synthesis was performed using the SensiFAST™ cDNA Synthesis Kit (Bioline) and RNA concentration levels and purity were determined using a NanoDrop spectrophotometer (Thermo-Scientific) ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Real-time quantitative PCR was performed using the SensiFAST™ SYBR® No-ROX Kit (Bioline) combined with a LightCycler® 480 Real-Time PCR System (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: RNA was isolated using the ISOLATE II RNA Mini Kit (Bioline) following manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... washed with PBS and total RNA extracted (Isolate II RNA mini kit, Bioline). cDNA was synthesized from 1 μg of total RNA using Oligo (dT ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 μg of total RNA using Oligo (dT) and Super Script Reverse Transcriptase III (Bioline). GoTaq qPCR Master Mix (Thermofischer ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA extraction from tail snips and genotyping PCR reactions were performed using MyTaq Extract-PCR kit (Bioline, Taunton, MA). All the mice were genotyped by the fragment PCR method as indicated in the Jackson Laboratory website ...
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bioRxiv - Neuroscience 2021Quote: ... In short myTaq extract-PCR kit (Bioline) was used to amplify a 300 bp fraction of the ath5 gene containing the lakritz mutation using the following primers ...
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bioRxiv - Neuroscience 2021Quote: ... the PyroTaq EvaGreen qPCR Master mix (Cultek Molecular Bioline) and the following primers (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 8 µl of primer pairs (0.5 µM each) and 10 µl of SensiMix SYBR & fluorescein mastermix (Bioline). The protocol included a 10 minute step at 95°C followed by 40 cycles at 95°C for 10 s and 60°C for 15 s ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR and RT-qPCR were performed according to the manufacturer’s protocols using MyTaq™ Red Mix (Bioline/BIOCAT) for RT-PCR and GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 1 μg of purified RNA with a SensiFast cDNA synthesis kit (Bioline). The primers used are listed in Table S2 (Supplementary Material) ...
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bioRxiv - Molecular Biology 2021Quote: ... and lysozyme were determined with a Real Time Thermal cycler qTower (Analytical Jena) system using a SensiFast SYBR kit (Bioline) according to the supplier’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR quantification of DNA samples was carried out using the SensiFAST SYBR Hi-ROX Kit (Bioline, BIO-92005) in 96-well plates using a ABI PRISM 7000 system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2021Quote: ... 100ng of each gene specific forward and reverse primer and 0.2μl of Biolase Taq polymerase (Bioline) in a 20μL reaction ...