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Citations for Bioline :
851 - 900 of 1617 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Real-time quantitative reverse transcriptase PCR (RT-qPCR) was performed using a Biorad CFX384 Optics Module with SensiFAST SYBER No-ROX (Bioline Meridian Biosystems) using gene-specific primers listed in Supplementary Table 1 ...
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bioRxiv - Plant Biology 2021Quote: Total RNA extracted from was extracted using TRIsure reagent (Bioline Meridian Biosystems). Total RNA (1-5 μg ...
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bioRxiv - Plant Biology 2021Quote: ... and reverse-transcribed using Tetro Reverse Transcriptase (Bioline Meridian Biosystems) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Total RNA was extracted by grinding snap frozen tissue to a fine powder in liquid nitrogen with a motor and pestle using TRIsure reagent (Bioline Meridian Biosystems), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... purified using TRIsure reagent (Bioline Meridian Biosystems), denatured and annealed prior to use.
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bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed with the SensiFastTM SYBR No-Rox Kit (Bioline) in a Roche Light Cycler 480 as described by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were diluted and 5 μl was used for genotyping PCR using MyTaq DNA polymerase (Bioline) with indicated DNA primers ...
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bioRxiv - Genetics 2021Quote: ... genomic DNA was extracted using the ISOLATE II Genomic DNA kit (Bioline). Following DNA amplification and fragmentation according to the associated Illumina HTS assay protocol samples were hybridized to an Infinium PsychArray v1.1 BeadChip (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: Ear biopsies from 21 days old mice were digested in MyTaq Extract PCR kit according to the manufacturer (Bioline, Cat number BIO-21126). PCR amplification was performed using the high-fidelity enzyme Q5 from New England Biolab (NEB ...
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bioRxiv - Genetics 2021Quote: ... 1 µg of total RNA was reverse transcribed using SensiFAST cDNA Synthesis Kit following the manufacturer’s protocol (Bioline).
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bioRxiv - Genetics 2021Quote: Quantitative polymerase chain reaction (qPCR) was performed using SensiFAST™ SYBR No-ROX Kit (Bioline, BIO-98020) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... purified using PureLink Quick Gel Extraction and PCR Purification Combo Kit” (Bioline) and eventually Sanger sequenced to finally prove their identity and the back-splice junction ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA synthesis was performed using the Tetro cDNA synthesis kit (Bioline). 2x GoTaq Green master mix (Promega ...
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bioRxiv - Microbiology 2021Quote: ... SureClean was applied to purify DNA or vector between steps as described by the manufacturer (Bioline GmbH, Luckenwalde, Germany). Finally ...
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bioRxiv - Neuroscience 2021Quote: ... 5μl SensiFAST SYBR Green No-ROX Kit (BIOLINE, BIO-98020), and 2μl of DNAse/RNAse-free water ...
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bioRxiv - Microbiology 2021Quote: ... Bands of approximately 350 bp were excised by comparing to a 50 bp ladder (Bioline), and subsequently purified using the MinElute Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... using 5 μl product with 1 μl 5X loading dye (BioLine) to verify bands of approximately 450 bp ...
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bioRxiv - Microbiology 2021Quote: For quantification of cellular TMPRSS2 and GAPDH expression cDNA synthesis and qPCR were performed according to the manufacturer’s instructions using the SensiFast cDNA kit and SensiFAST SYBR qPCR kit (both from Bioline). The qPCR was run on a StepOnePlus realtime PCR cycler (Thermo ...
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bioRxiv - Microbiology 2021Quote: ... 125nM) and SensiFAST Probe Hi-ROX One-Step Kit (Bioline) according to the manufacturer’s instructions in a 20μl reaction with 5μl sample ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was carried out using the SensiFAST SYBR Hi-ROX kit (Bioline, BIO92005). Briefly ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitated small RNA (bound by AGO4) was released from beads and isolated by TRIsure (Bioline) extraction ...
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bioRxiv - Plant Biology 2021Quote: ... and SensiMix™ SYBR® & Fluorescein Kit (Bioline, Catalog # QT615-05) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was performed in 20mL with 1mg of total RNA using the Tetro cDNA synthesis kit (Bioline). RT-negative (no enzyme ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR reactions using SYBR green chemistry (SensiFast; Bioline) were set up with a CAS-1200N robotic liquid handling system (Corbett Research ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR reactions were made using SensiFAST master-mix (Bioline), in 20 μl volumes ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed with 2x MyTaq HS Mix (Bioline), using the following Hnrnpu primers ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was reverse transcribed using the SensiFast cDNA Synthesis Kit (Bioline), and quantitative real-time PCR was performed using the LightCycler DNA Master SYBR Green I Kit in a LightCycler 480 System (Roche) ...
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Hexose translocation mediated by SlSWEET5b is required for pollen maturation in Solanum lycopersicumbioRxiv - Plant Biology 2021Quote: Total RNA was isolated from tomato organs with TRIsure reagent (Bioline, http://www.bioline.com/) along with an RNA purification column (GeneMark ...
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bioRxiv - Neuroscience 2021Quote: ... We synthesized complementary DNA (cDNA) from 100ng of RNA in a 40μl reaction containing 100 units of Tetro Reverse transcriptase (Bioline, Taunton, MA), 8μl of 5X RT buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 1mM dNTP Mix (Bioline), 40 units of Ribosafe RNAse Inhibitor (Bioline) ...
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bioRxiv - Neuroscience 2021Quote: ... 40 units of Ribosafe RNAse Inhibitor (Bioline), and 4μl of 10X random primers (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... the SensiFASTTM SYBR No-ROX Mix (Bioline), and the following primers ...
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bioRxiv - Microbiology 2021Quote: ... and real-time qPCR was performed using SensiFast SYBR Lo-ROX kit (Bioline). RT-qPCR was carried using primer sets for the three reporter genes and three housekeeping genes (actin I (p100 ...
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bioRxiv - Microbiology 2021Quote: ... cDNA preparation was performed using SensiFAST cDNA Synthesis Kit (Bioline) and real-time qPCR was performed using SensiFast SYBR Lo-ROX kit (Bioline) ...
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bioRxiv - Microbiology 2021Quote: ... or SensiFAST SYBR Hi-ROX kit (Bioline). Reactions were run using Applied Biosystems 7500 fast RT-PCR machine (Thermofisher ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR analyses were performed using Brilliant III SYBR Green QPCR master mix (Bioline) with gene-specific primers according to the manufacturer’s protocol and the Applied Bioscience StepOnePlus qRT-PCR machine (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... and 2× My Taq Red (Bioline, Taunton, MA, USA). The PCR conditions were as follows ...
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bioRxiv - Microbiology 2021Quote: ... 1:10 dilutions of RNA samples were analyzed using SensiFast SYBR Hi-ROX One-Step kit (Bioline, UK) and 400 nM primer concentrations ...
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bioRxiv - Microbiology 2021Quote: ... Four oligonucleotides were designed [40] for PCR using MyTaq Red Mix 1X (Bioline, Cat#BIO-25043), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were analysed by 0.8% agarose-gel electrophoresis with 1 kb ladder (Bioline, Cat#H1-819101A), run at 100 V for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... and DNA purification and plasmid isolation kits were obtained from Bioline (Cat#BIO-52060 and Cat#BIO-52057 ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was treated with 10 µL 20 mg/mL Proteinase K (BIOLINE, Cat#BIO-37037) and purified using the Norgen Phage DNA Isolation Kit (cat ...
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bioRxiv - Microbiology 2021Quote: ... The DNA was precipitated from fractions by adding 4μl of Co-precipitant Pink Linear Polyacrylamide (Bioline) and 2 volumes of PEG-NaCl solution (30% w/v polyethylene glycol 6000 ...
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bioRxiv - Microbiology 2021Quote: ... Two micrograms of total RNA was reverse-transcribed with Sensi-FAST™ cDNA Synthesis Kit (Bioline, Memphis, USA). The amounts of p16 ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated using SensiFast cDNA synthesis kit (Bioline) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... For qPCR reaction 1 μg of cDNA was used with SensiFast Sybr Lo-Rox mix (Bioline) and respective primer pair ...
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bioRxiv - Cancer Biology 2021Quote: RNA was extracted using Isolate II Mini kit (Bioline), following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... A DNA contamination PCR was performed using MyTaq™ DNA Polymerase (Bioline cat # BIO-21105) and primers for ACTB to check for the presence of genomic DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The final volume contained 1× SensiMix (Bioline, London, UK), 800 nM of each primer and 200nM of each probe and the PCR cycling conditions included an initial denaturation at 95°C for 10 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... The DNA was used as template for PCR amplification of the targeted sites with primers listed on Supplementary Table 3 and using MyTaq™ DNA Polymerase (Bioline). Subsequently ...