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Citations for Bioline :
1251 - 1300 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from treated cells via an ISOLATE II RNA mini kit (Bioline, Cat: BIO-52073) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and a SensiFast Probe kit (Bioline). Expression of tubulin was used to normalize the expression of target genes ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated using an Isolate II Rna Mini Kit (Bioline). 250~500 ng of RNA was reverse transcribed into cDNA using the High-Capacity cDNA reverse transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR analyses were performed using Brilliant III SYBR green QPCR master mix (Bioline) with gene-specific primers according to the manufacturer’s protocol and with the Applied Bioscience StepOnePlus qRT-PCR machine (Life Technologies) ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR was performed with 20 µL Mango Mix™ (Bioline), 0.25 µM of each primer and 2 µL of DNA template in a final volume of 40 µL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 μL of MyTaq™ 5x reaction buffer (Bioline), 0.08 μL of each primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR reactions were carried out in a volume of 15 μL including 0.25-1.0 U of MyTaq™ HS DNA polymerase (Bioline), 2 μL of MyTaq™ 5x reaction buffer (Bioline) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1x MyTaq™ reaction buffer and 1.0 U of MyTaq™ DNA polymerase (Bioline Meridian Bioscience) adjusted to a total volume of 20µL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2 μL of diluted cDNA were combined with 5 μL SYBR Green (Bioline, Luckenwalde, Germany), 0.2 μL of each forward and reverse primer and 4.6 μL DEPC-water ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For each gene a total of 8 clones from each plant were isolated from overnight LB cultures using an ISOLATE II Plasmid Mini Kit (Bioline) prior to sequencing with universal M13F and M13R primers by GATC Biotech (Konstanz ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Total RNA was extracted using a Bioline ISOLATE II RNA Plant Kit (Bioline Ltd, London, UK), following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... lyrata PER plants as above and a short section of ASY3 was PCR amplified using 0.5 μM primers (S5 Table) and MyTaq™ Red Mix (Bioline). PCR conditions were as follows ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1U MyTaq polymerase (Bioline, Australia), 0.2 μM of each PCR primer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.5 U MyTaq polymerase (Bioline, Australia), 0.1 μM of forward primer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Every pair of dissected pectoral fins coming from a single larva was transferred to a separated 1.5 ml tube containing 50 μl of TRIsure (BIO-38032, Bioline) and kept at −20°C in until larvae were genotyped (see in situ hybridization section) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 11.25 μl SensiMix SYBR No-ROX Master Mix (Bioline, Australia) was added ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Telomere length was measured using real-time quantitative PCR (qPCR) and a SensiMix SYBR No-ROX Kit (Bioline, Sydney, Australia). The telomere primers used were Tel1b (5’-CGGTTTGTTTGGGTTTGGGTTT GGGTTTGGGTTTGGGTT-3’ ...
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bioRxiv - Developmental Biology 2020Quote: ... Cross-linking was reversed by overnight incubation at 60°C with proteinase K (Bioline, Lot # BIO-37037). Then 3C libraries were purified by phenol-chloroform followed by chloroform extraction and ethanol-precipitated at −80°C overnight ...
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bioRxiv - Evolutionary Biology 2020Quote: ... consisting of 5 μL MyTaq HS DNA Polymerase (Bioline), 0.2 μL of each forward and reverse primer ...
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bioRxiv - Developmental Biology 2020Quote: ... manually de-chorionated and suspended in TRIsure (Bioline) with chloroform ...
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bioRxiv - Developmental Biology 2020Quote: ... or GST were transfected in BL21 (DE3) Escherichia coli bacteria (Bioline). Bacterial starter culture was made by inoculation of 4 mL Luria broth with 10 μg/mL ampicillin ...
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bioRxiv - Developmental Biology 2020Quote: ... and mClover3-bElys constructs were cloned from bovine fibroblast or bovine oocyte cDNA libraries made using a SensiFAST cDNA synthesis kit (Bioline, BIO-65053). The primers (KASH5-DN cloning ...
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bioRxiv - Developmental Biology 2020Quote: ... qPCR was performed with SensiFast Sybr green (BIOLINE). For each genotype ...
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bioRxiv - Developmental Biology 2020Quote: ... one μg of each template was synthesized into cDNA with a standard reaction mix (SensiFAST™ cDNA Synthesis Kit, Bioline) in a thermal cycler (Bio-Rad T100TM Thermal Cycler ...
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bioRxiv - Microbiology 2020Quote: ... The PCR reaction volumes are as follows: 12.5 μL MyTaqTM Master Mix (Bioline, United Kingdom), 10 μL molecular-grade water (G-Biosciences ...
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bioRxiv - Genomics 2020Quote: ... Pluripotency and early differentiation marker as well as selected known imprinted genes expression was determined by qPCR using the Sensifast SYBR No Rox-Kit (Bioline).
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bioRxiv - Genomics 2020Quote: ... RNA was reverse-transcribed into cDNA using the SensiFAST cDNA Synthesis Kit (Bioline). Pluripotency and early differentiation marker as well as selected known imprinted genes expression was determined by qPCR using the Sensifast SYBR No Rox-Kit (Bioline).
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bioRxiv - Molecular Biology 2020Quote: ... For RT-qPCR cDNA was prepared from 1 µg of total RNA using the SensiFAST cDNA Synthesis Kit (BIO-65054, Bioline) and analysed by qPCR using the SensiFast SYBR Lo-ROX Kit (BIO-94050 ...
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bioRxiv - Molecular Biology 2020Quote: ... and analysed by qPCR using the SensiFast SYBR Lo-ROX Kit (BIO-94050, Bioline) according to manufacturer’s instructions together with gene-specific primers ...
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bioRxiv - Molecular Biology 2020Quote: ... coli as per manufacturer’s protocol (α-Select Competent Cells, Bioline, Australia). The transformed cells were grown overnight (37° ...
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bioRxiv - Molecular Biology 2020Quote: Complementary DNA (cDNA) used for in situ hybridisation and quantitative PCR (qPCR) was synthesised using the Tetro cDNA synthesis kit (Bioline, London, UK) with oligo-dT primers according to the manufacturers’ instructions.
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bioRxiv - Molecular Biology 2020Quote: ... DNA was extracted using alkaline lysis after which the EntF* fragment was amplified using 2x Biomix (Bioline, Belgium) in a Mastercycler PCR system (Eppendorf ...
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bioRxiv - Genomics 2020Quote: ... at 37°C for 30 min and then proteinase K digestion (Bioline, cat. #37084) for 2 h at 56 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Each 100 μl PCR reaction contained 5 U MyTaq DNA polymerase (Bioline, Australia), 1 × MyTaq reaction buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The Sensifast Probe No-Rox mastermix (Bioline, Australia) was chosen for future testing.
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bioRxiv - Molecular Biology 2020Quote: ... The SensiMix SYBR No-ROX kit was from Bioline (London, UK). SmaI was from New England Biolabs (Ipswich ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by qPCR using SensiMix SYBR (Bioline, UK) and KAPA Illumina DNA standards (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline). The U6 snRNA gene was used as an internal control.
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bioRxiv - Molecular Biology 2020Quote: ... cDNA products were used as the template for quantitative reverse transcription-PCR (qRT-PCR) using the SensiMix SYBR No-ROX kit (Bioline). For relative quantification of gene expression ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA isolation was carried out using TRIsure™ reagent according to the manufacturer’s instructions (Bioline Reagent, UK). The yield ...
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bioRxiv - Molecular Biology 2020Quote: ... For RT-qPCR the SensiFAST SYBR Lo-ROX Kit (Bioline) was used ...
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bioRxiv - Microbiology 2020Quote: ... and their molecular weight was assessed by comparison to a molecular marker (Hyperladder IV, Bioline, London, UK).
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bioRxiv - Microbiology 2020Quote: PCR products were purified using a commercial kit (Isolate II PCR and Gel Kit, Bioline, London, UK) and sent for sequencing (Macrogen Europe ...
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bioRxiv - Genomics 2020Quote: ... and column purified using ISOLATE II RNA Mini Kit (Bioline, Australia) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... but without gel purification and with use of the Velocity DNA Polymerase (BioLine, Luckenwalde, Germany) for 25 cycles of amplification ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using the SensiFAST SYBR No-ROX One-Step Kit (Bioline) and primers listed in Table S1 ...
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bioRxiv - Genomics 2020Quote: ... One-step reverse-transcription quantitative PCR (RT-qPCR) was prepared with 50 ng of RNA using the SensiFAST™ SYBR® No-ROX One-Step kit (Bioline) on an Eco™ Real-Time PCR System (Ilumina) ...
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bioRxiv - Genomics 2020Quote: ... and converted to cDNA using Tetro cDNA synthesis kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Realtime RT-PCR was carried out on an Applied Biosystems 7900HT using SensiMix SYBR green master mix (Bioline). 300nM each primer were added to 10μl reactions in 384-well plates ...
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bioRxiv - Microbiology 2020Quote: ... 2uL of the DNA extracted from sputum samples was used as a template in 20-mL qPCR reactions containing 1× SensiFAST™ Probe Hi-ROX (Bioline, Cat. No. BIO-82020), 200 nM probe ...