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Citations for Bioline :
901 - 950 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Resulting PCR products were purified on columns (Isolate II PCR Kit (BIO-52059, Bioline)) that are suitable for >50 bp DNA fragments ...
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bioRxiv - Molecular Biology 2021Quote: ... a SensiFAST™ SYBR® No-ROX Kit (Bioline, London, UK) was used to perform the qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... and one μg of total RNA from each sample was converted into cDNA with SensiFAST™ cDNA Synthesis Kit (Bioline, London, UK) using a T100™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... a 10 μl qPCR reaction is set up using SensiFAST SYBR No-ROX kit (FroggaBio/Bioline, catalogue number BIO-98050), forward and reverse primers (Table 2) ...
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bioRxiv - Molecular Biology 2021Quote: ... consisting of 6.25 μL of MyTaq Red Mix (Bioline), 0.075 μL of forward primer (10 μM) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was reverse transcribed to cDNA using SensiFAST cDNA synthesis kit (Bioline). The cDNA was diluted 1:5 with nuclease free water and 5 μL were used per qPCR reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified RNA was then reverse transcribed into complementary DNA (cDNA) with the SensiFAST™ complementary cDNA synthesis Kit (Bioline) and used in qPCR ...
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bioRxiv - Molecular Biology 2021Quote: qPCRs were performed using SensiFastTM SYBR® Lo-ROX kit (Bioline). A master mix for each primer pair was prepared following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR reactions were set up using sensiFAST SYBR Lo-ROX kit (Bioline). The qPCR was performed as described above for RIP-qPCR.
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bioRxiv - Molecular Biology 2021Quote: RNA was purified using TRIsure (Bioline, BIO-38033) and reverse transcribed using the Im-Prom-II Reverse Transcription System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... and quantitative real-time-PCR (qRT-PCR) reactions were performed in triplicate (Bioline) and monitored by a Roche Lightcycler 96 ...
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bioRxiv - Immunology 2021Quote: ... Participants had positive rapid diagnostic test for malaria (SD BIOLINE, Malaria Ag P. f., Abbott) at the field site ...
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bioRxiv - Immunology 2021Quote: RNA isolation (Bioline) and cDNA synthesis (Roche ...
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bioRxiv - Immunology 2021Quote: ... SYBR green (Bioline) signal was detected on the BIO-RAD analyzer and normalized to GAPDH using the Pfaffl formula ...
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bioRxiv - Molecular Biology 2021Quote: ... Captured libraries were quantified by qPCR using SensiMix SYBR (Bioline, UK) and KAPA Illumina DNA standards (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by qPCR quantification using SensiMix SYBR (Bioline, UK) and KAPA Illumina DNA standards (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... Enrichment of ChIP DNA at predicted sites for each chromatin modification was confirmed by qPCR using primers given in the Key Resources Table and SensiMix SYBR (Bioline, UK). 25 – 100 ng of ChIP DNA was used for library prep using the NEBNext Ultra II DNA Library Prep Kit with NEBNext Single indices (E7645).
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bioRxiv - Molecular Biology 2021Quote: ... and SensiMix SYBR (Bioline) before being pooled together ...
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bioRxiv - Molecular Biology 2021Quote: ... Ago2 and Gapdh were quantified using sensiFAST SYBR No ROX qPCR kit (Bioline). Homer and Snord15b were used to estimate the efficiency of soma/neurite separation ...
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bioRxiv - Physiology 2021Quote: ... SensiFAST SYBR Green No-ROXSYBRgreen (Bioline BIO-94002) was used on ViiA7 Real-Time PCR systems (ABI) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR mix was composed of 5 μL of 5X MyTaq red PCR buffer (Bioline), 1.25 μL of 25 mM MgCl2 ...
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bioRxiv - Genetics 2021Quote: ... Primers flanking the MT-ND4 gene were used to amplify the edited locus using MyTaq polymerase (Bioline, USA). PCR amplicons were gel extracted and purified using QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Genetics 2021Quote: ... MgCl and Taq polymerase using Mytaq Master Mix (Bioline). The PCR reaction condition is as follows ...
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Genomic and phenotypic characterization of finger millet indicates a complex diversification historybioRxiv - Genetics 2021Quote: ... Genomic DNA (gDNA) was extracted from finely-ground leaf material using the ISOLATE II Genomic DNA Kit (Bioline Pty Ltd) and according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... qRT-PCR was carried out on 1:10 diluted cDNA on an Applied Biosystems StepOnePlus system using Sensifast Hi-Rox Sybr kit (Bioline). Cycle conditions were as follows ...
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bioRxiv - Immunology 2021Quote: ... The PCR reactions were assembled using 45 μL master mix (Bioline) containing 2x buffer ...
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bioRxiv - Immunology 2021Quote: ... and RNA was extracted using Isolate II RNA Mini or Micro Kit (Bioline, UK). An amount of 5 ng of total RNA per sample was used for transcriptome amplification using NuGEN’s Ovation RNA-Seq V2 kit (San Carlos ...
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bioRxiv - Immunology 2021Quote: Liver samples were collected in TRIzol for RNA extraction and cDNA synthesis (Bioline) using standard protocols ...
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bioRxiv - Immunology 2021Quote: ... RNA (500 ng) was reverse-transcribed into cDNA using the SensiFAST cDNA Synthesis Kit (BioLine) according to the manufacturer’s specifications for synthesis of cDNA for use in RT-qPCR reactions ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCRs were performed using Sensimix SYBR NORox SYBR GREEN (Bioline, UK). Each PCR was performed in duplicate using 1 μl of cDNA (from 20 μl reaction ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Hypothalamic nuclei punches were weighed and then stored in 200 µL of TriSure reagent (BioLine) at -80 until RNA extraction.
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and gonad samples using TriSure (BioLine) along with a modified protocol of the Zymo Direct-zol RNA miniprep spin- column extraction kit (Zymo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... using Sensifast SYBR green (Bioline) in a 20 μL reaction according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized using Sensifast (Bioline) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and IMMOLASE™ DNA polymerase (5 U/μl) (Bioline; Cat. No. BIO-21047) as described by Lee et al ...
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bioRxiv - Microbiology 2021Quote: ... and 3 μL of Proteinase K (20 mg/ mL, Bioline Australia Pty Ltd ...
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bioRxiv - Microbiology 2021Quote: ... using First strand cDNA synthesis kit (Bioline, UK), 1 μg of total RNA was converted into cDNA ...
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bioRxiv - Microbiology 2021Quote: ... RNA was reverse transcribed with the Tetro cDNA synthesis kit (Bioline) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction was done using either the Bioline DNA extraction kit (Bioline Corporation, London, UK) or the CTAB (Cetyl Trimethyl Ammonium Bromide ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA samples were run in triplicate in 96 well reaction plates using SYBR-Green-based qPCR (SensiFast, HI-ROX, Bioline) according to manufacturer’s instructions using a StepOnePlus System (Applied Biosystems ...
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bioRxiv - Zoology 2021Quote: ... 2.4 U IMMOLASE DNA Polymerase (Bioline, London, UK) and 3 µL diluted DNA ...
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bioRxiv - Systems Biology 2021Quote: ... The double stranded cDNA was purified using JetSeq Beads (Bioline, USA). Purified cDNA was end-repaired ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... Cells were then lysed in hypotonic lysis buffer containing 0.16 U µl−1 Ribosafe RNase inhibitors (Bioline), 2 mM PMSF and SIGMAFAST Protease Inhibitor Cocktail tablets ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... 1 µg RNA (iNeurons samples) or 2 µg RNA (Drosophila samples) was converted to cDNA using BioScript Reverse Transcriptase (Bioline). qRT–PCR primers were designed using Origene or Primer-BLAST and validated using a 1 in 4 serial template dilution series (standard curve with R2>0.97) ...
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bioRxiv - Bioengineering 2021Quote: ... and Velocity DNA polymerase (Bioline) were used according to the manufacturers’ instructions ...
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bioRxiv - Biochemistry 2021Quote: ... RT-qPCR was carried out using SensiMix™ SYBR® and Fluorescein kit (QT615-05; Bioline, London, United Kingdom). The following primers were used ...
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bioRxiv - Biochemistry 2021Quote: ... total cellular RNA was isolated using the Isolate II RNA kit (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Bioline cDNA synthesis kit (Bioline, London, UK) was utilised to synthesize cDNA from 1 μg of total RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... reverse-transcribed cDNAs were amplified in duplicate using SensiFast SYBR (Bioline). Real-time quantitative reverse transcription (qRT)-PCR analysis was performed on a CFX96 real-time system (Bio-Rad Laboratories Srl ...
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bioRxiv - Cell Biology 2021Quote: ... and E-gene expression assessed using the SensiFAST Probe No-Rox One Step Kit (Bioline) and the following primers/probes ...