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Citations for Bioline :
1201 - 1250 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Libraries of cDNA were created using SensiFAST cDNA Synthesis Kit (Bioline). Control reactions without reverse transcriptase were conducted to confirm the absence of contaminating DNA in all samples ...
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bioRxiv - Microbiology 2021Quote: qRT-PCR was performed using the SensiFAST Probe Lo-ROX One-Step kit (Bioline Meridian, Australia) with a reaction volume of 20µl ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed in triplicates using SensiFAST SYBR No-ROX Kit (Bioline) in Rotor-Gene 6000 (Corbett Life Science) ...
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bioRxiv - Cell Biology 2021Quote: ... and all the used strains were genotyped before its use using MyTaqTM DNA polymerase (Bioline) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The reagents used are TRIsure (Bioline, England), SYBR (Bioline ...
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bioRxiv - Cell Biology 2021Quote: The total RNA was extracted using TRIsure (Bioline, England). Prepared cells were distributed into an 8 pi medium consisting of 1 × 106 cells on 100 pi plates ...
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bioRxiv - Cell Biology 2021Quote: ... and the SensiFAST SYBR Lo-Rox Kit (Bioline, England). The sequences of primers used in the experiment are presented in Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... SYBR (Bioline, England), Reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: mRNA was isolated using NucleoSpin® RNA Plus (Machery-Nagel) and 1 µg of total mRNA was reverse-transcribed into cDNA using SensiFASTTM cDNA Synthesis Kit (Bioline) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA (100 ng) was analysed by quantitative PCR using SensiFASTTM SYBR Lo-ROX Kit (Bioline) in the 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Genomics 2021Quote: ... cDNA was synthesized using Bioscript reverse transcriptase (Bioline), Random Primers (Thermo Fisher) ...
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bioRxiv - Genomics 2021Quote: ... qPCR amplification was performed in triplicate with SYBR green qPCR chemistry (Bioline) using primers for Tnfa (F-5’- ACCTCACACTCAGATCATCTTCTC-3’ ...
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bioRxiv - Genomics 2021Quote: ... Amplification was performed in duplicate using SYBR green qPCR chemistry (Bioline) using a universal barcode forward primer (F-5’- GCTTGGTTAGAATGGGTAACTAGTTTGCAG-3’ ...
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bioRxiv - Genomics 2021Quote: ... ans Isolate II Genomic DNA kit (Bioline) was used to extract genomic DNA from 1×107 cells ...
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bioRxiv - Genomics 2021Quote: ... One-step reverse-transcription quantitative PCR (RT-qPCR) was performed with the SensiFAST™ SYBR® No-ROX One-Step kit (Bioline®) on an Eco™ Real-Time PCR System (Ilumina® ...
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bioRxiv - Molecular Biology 2021Quote: ... Each sample was measured in triplicate of 5 μl with the following final concentrations: Velocity Polymerase 0.6 U (Bioline), 1.2 x Hifi Buffer (Bioline) ...
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bioRxiv - Immunology 2021Quote: ... the cDNA was synthesized using SensiFAST reverse transcriptase (Bioline), and quantitative PCR was run using SensiFAST Sybr Master Mix (Bioline) ...
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bioRxiv - Immunology 2021Quote: ... and quantitative PCR was run using SensiFAST Sybr Master Mix (Bioline). The primers used to detect GFP were a1 5′-caagggcgaggagctgttca-3′ and 5′-tgaacttgtggccgtacgtcg-3′ (GFP7) ...
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bioRxiv - Molecular Biology 2021Quote: ... and SensiFAST SYBR Hi-ROX mix (Bioline). Total reaction volume was 10 µl with 150 nM of each primer ...
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bioRxiv - Immunology 2021Quote: ... obtained from the TaqMan RT-PCR kit (Bioline cat# BIO-78005), RT ...
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bioRxiv - Immunology 2021Quote: ... 5µl of 10 mM dNTP mix (Bioline, Catalogue no.-BIO-39044), and 1µl of RNaseOUTTM Recombinant Ribonuclease Inhibitor (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR was carried out using the Bioline SensiFASTTM Probe Lo-ROX Kit (Bioline, Catalogue no.-84005) and Stratagene Mx3005P (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-quantitative PCR (RT-qPCR) was performed using a SensiMix SYBR kit (Bioline, #QT605-05) on an Applied Biosystems ViiATM7 Real-Time PCR machine ...
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bioRxiv - Microbiology 2021Quote: ... Total cDNA was prepared by the SensiFAST™ cDNA Synthesis Kit (Bioline, Memphis, USA) and analyzed by next generation sequencing service (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... After reverse-transcription by the SensiFAST™ cDNA Synthesis Kit (Bioline, Memphis, USA), cDNA samples were prepared with the QuantiFast SYBR Green PCR Master Mix reagent (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... cells were collected by centrifugation at 4000 rpm for 15 minutes and pellets used for RNA extraction by RNeasy kit according to the manufacturer’s protocol (Bioline). cDNA synthesis and RT-qPCR was performed as previously described (60) ...
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bioRxiv - Plant Biology 2020Quote: ... SYBR Green reaction mix (Bioline; Sensimix SYBR No-ROX kit) was used in a Bio-Rad CFX384 real-time system for qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... RNA extraction was carried out using Trisure® (Bioline, Australia) by following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA library construction was performed using SensiFAST™ cDNA Synthesis Kit (Bioline, Australia).
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bioRxiv - Plant Biology 2020Quote: The RT-qPCR experiments were carried out using SensiFAST™ SYBR No-ROX Kit (Bioline, Australia). Each reaction contains 5 µl SensiFAST mix ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was carried out with SensiFAST(tm) SYBR® & Fluorescein Mix (BIOLINE) in a Bio-Rad iQ5 thermal cycler ...
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bioRxiv - Plant Biology 2020Quote: ... Immolase (Bioline, Memphis, USA), 300 nM of a gene-specific forward and reverse primer and 2 μl of a 1:10 diluted cDNA in a 20 μl reaction ...
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bioRxiv - Plant Biology 2020Quote: ... the SensiFAST SYBR No-ROX Kit was used according to manufacturer’s recommendations (Bioline Reagents Ltd., London, UK). Primers used for semi quantitative RT-PCR can be seen in Table 2.
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bioRxiv - Plant Biology 2020Quote: ... RNA extraction was performed using the Isolate II RNA Plant kit (Bioline). During RNA extraction two samples were combined by using the same lysis buffer on two samples to make up one biological replicate ...
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bioRxiv - Microbiology 2020Quote: ... 250-1,000 ng of extracted RNA was converted to cDNA in a reaction volume of 20 μl using the Tetro cDNA synthesis kit (BIO-65043, Bioline, London, UK). The cDNA was then amplified using an SYBR green RT-PCR kit (06924204001 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sensifast Hi-Rox SyBr kit (Bioline) was used to perform the RT-qPCR on a StepOnePlus system (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... coli α-Select competent cells were obtained from Bioline Reagents Ltd (UK) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA synthesis was performed with 0.25-1 µg of RNA in a 20 µL total reaction volume using a random hexamer/oligo dT strand synthesis kit as per the manufacturer’s instructions (10 min at 25°C; 15 min at 42°C; 15 min at 48°C; SensiFast, Bioline). All oligonucleotide sequences are listed in Table 1.
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bioRxiv - Systems Biology 2020Quote: ... Transcripts were quantified using SensiFAST SYBR (BioLine) on an LC480 instrument ...
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bioRxiv - Molecular Biology 2020Quote: The CAN1 locus from genomic DNAs of mutants was amplified in a 96-well format using MyTaq DNA polymerase (Bioline). Each PCR product was uniquely barcoded using primers that contained 20 nt of CAN1-specific sequence conjugated to 16 nt of PacBio forward or reverse barcodes (https://github.com/PacificBiosciences/Bioinformatics-Training/blob/master/barcoding/pacbio_384_barcodes.fasta) ...
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bioRxiv - Neuroscience 2020Quote: ... 5 µl of (2x) SYBR green master mix (SensiFAST SYBR kit, Bioline, London, United Kingdom; BIO- 94005) was used and combined with 0.4 µl of a combined forward and reverse primer mix (10 uM of forward and reverse primers in the combined master mix ...
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bioRxiv - Cell Biology 2020Quote: ... or 50 µg/mL of Proteinase K (Bioline) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... using SensiMix™ SYBR® No-ROX Kit (Bioline) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was used to analyse differences in gene expression by qRT-PCR employing SensiMix SYBR low-Rox KIT (Bioline, Germany) along with gene specific forward and reverse primers (250 nM ...
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bioRxiv - Cell Biology 2020Quote: ... PCR bands were extracted from the agarose gel and purified using and Isolate II PCR and gel kit (Bioline, London, UK) and sent for Sanger sequencing (GATC Biotech ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from cell pellets using the Isolate II RNA Mini Kit (Bioline) RNA extraction Kit following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... PDZ4 (1099–1203)) as well as Scribble mutants were expressed using Escherichia coli BL21 (DE3) pLysS cells (BIOLINE) as Glutathione S-transferase or Maltose Binding Protein fusions (Rautureau et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The relative amount of PAK transcript variants was measured by quantitative real-time PCR (qPCR) using SensiFAST SYBR No-ROX Kit (Bioline) and calculated using Bio-Rad CFX Manager Software ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR was performed using the SensiFAST SYBR No-ROX Kit (Bioline). Primers used to quantify FLOE1 expression were priqPCRFLOE1set1-FWD/REV (Table S3) ...
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bioRxiv - Cell Biology 2020Quote: ... Then 5 μg of the extracted RNA was reversely transcribed by utilizing Tetro cDNA Synthesis Kit (Bioline, BIO-65043) and random Hexamer Primer according to the manufacturer’s protocols ...