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Citations for GE Life Sciences :
601 - 650 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were sonicated and His-tagged proteins were purified using affinity chromatography (HisGraviTrap column from GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was first affinity-purified on an ÄKTA Purifier using His-Trap HP columns (GE Healthcare) and eluted with elution buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... All mAbs were purified from cell culture supernatant using a HiTrap protein G HP affinity column (GE Healthcare) and stored at −80 °C ...
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bioRxiv - Immunology 2021Quote: ... RBD was purified from the supernatants by nickel affinity chromatography with HisTrap Excel columns (GE Healthcare Life Sciences). For protein production used in crystallization trials ...
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bioRxiv - Microbiology 2021Quote: ... The apparent binding affinity (KD) for individual antibody was calculated using BIAcore® 3000 Evaluation Software (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... The vector was purified from the clarified lysate by affinity chromatography using a HiTrap heparin column (GE Healthcare), ultracentrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Biochemistry 2023Quote: ... His6-HsCHIP21-154-TPR was purified by Ni2+-affinity chromatography using a 5mL HisTrap HP column (GE Healthcare) loaded in 25 mM HEPES pH 7.5 and 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... proteins were separated by immobilized-metal affinity chromatography (IMAC) on a 5 mL HisTrap HP column (GE Healthcare), previously washed with ddH2O and equilibrated with buffer B (25 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The bNAb affinity columns were made by cross-linking IgG to activated CNBr-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s instructions and as previously described (83) ...
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bioRxiv - Bioengineering 2023Quote: ... The ErCas12a protein in clarified lysate was sequentially purified using immobilized metal affinity chromatography (HisTrap HP, GE Healthcare) and heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion protein was purified by affinity chromatography on glutathione-Sepharose (GE Healthcare Life Sciences (now Cytiva)) in 300 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Molecular Biology 2023Quote: Surface plasmon resonance (SPR) affinity and kinetic analysis was carried out using the Biacore T200 instrument (GE Healthcare). aMino sdAbs were immobilized to a CM5 sensor chip at a density of 4300-4600 RU ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded on an affinity chromatography column (GE Healthcare, His-Trap FF crude, #17-5286-01) with a flow rate of 1 mL/ min ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered at 0.8 µm and used for purification by nickel ion affinity chromatography (GE Healthcare). A washing step in the lysis buffer supplemented with 30 mM imidazole was followed by the elution in lysis buffer supplemented with 500 mM imidazole ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2024Quote: ... and fermentation supernatants were purified by Protein-A affinity chromatography using MabSelect™ column (GE Healthcare, Princeton, NJ). The bispecific antibody was further polished by cation exchange chromatography using Capto MMC (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... a second nickel-affinity chromatography was performed (His Trap, 5 mL, GE Healthcare; equilibrated in 10% HisB buffer). Sample was loaded and unbound protein further washed out with 10% HisB ...
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bioRxiv - Neuroscience 2024Quote: ... We then purified filtrates using the pre-equilibrated HiTrap Heparin HP affinity columns (GE HealthCare, Chicago, IL, USA), followed by washing with the following buffers in sequence ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (1×PBS ...
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bioRxiv - Plant Biology 2023Quote: ... The soluble fraction was loaded on a Ni2+ affinity column (HisTrapTM Chelating HP Column, GE Healthcare, Uppsala, Sweden) using a peristaltic pump with a flow rate of 1 mL min-1 ...
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bioRxiv - Biophysics 2024Quote: ... a 2-elution step Ni2+-affinity chromatography was carried out using a Histrap HP 5 ml (GE Healthcare) on an FPLC apparatus (ÄKTA Pure ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Biochemistry 2021Quote: ... A radioactively labeled probe corresponding to the coding region of TbGPI2 was generated using a Megaprime DNA-labeling system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Biophysics 2022Quote: ... ELISA was performed as described2 using an HRP-conjugated anti-M13 monoclonal antibody (GE Healthcare) and a TMB substrate kit (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: Phototransduction activity was assessed by measuring the light-induced hydrolysis of endogenous cyclic (c) GMP with an enzyme immunoassay kit (Biotrack EIA system) according to the manufacturer’s instructions (Amersham Bioscience GE Healthcare). Undifferentiated WTCAct or WTCUDC iPSC and cells differentiated into RS were kept in the dark ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Bioengineering 2022Quote: ... Fc fragments and undigested IgG were removed from binding to the protein A beads (GE Healthcare). CC12.1 and eCC12.1.7 Fab fragments were generated by introducing a stop codon after the CH1 region in heavy chain expression plasmids ...
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bioRxiv - Microbiology 2020Quote: ... The rS1-Fc was protein A purified using MabSelect SuReLX resin (# 1754702) obtained from GE Healthcare.
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bioRxiv - Immunology 2023Quote: ... and Fc using size exclusion chromatragraphy with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated using 100 kDa Amicon concentrators ...
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bioRxiv - Molecular Biology 2020Quote: ... All GST-B3 fusion proteins were expressed in E.coli BL21(DE3) cells and were purified by gluthathione-Sepharose affinity chromatography according to the manufacturer’s recommendations (GE Healthcare). The proteins were eluted in elution buffer (20 mM reduced glutathione ...
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bioRxiv - Molecular Biology 2020Quote: Affimer affinities for KRAS were determined by surface plasmon resonance (SPR) using a BIAcore 3000 (GE Healthcare Europe GmbH). Affimer proteins with a C-terminal cysteine residue were biotinylated with the biotin-maleimide (SigmaAldrich ...
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bioRxiv - Biochemistry 2020Quote: ... purification of the protein was performed with a histidine affinity column (HisTrap FF crude 5 ml, GE Healthcare Amersham) taking advantage of the natural His present in the octapeptide repeat region of PrP ...
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bioRxiv - Cell Biology 2019Quote: ... the GST-PACRGΔ1-69:MEIG1 complex was purified by affinity chromatography using 1.5 mL of glutathione-sepharose resin (GE Healthcare) washed in Buffer A (50 mM of HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... The lysate was centrifuged and the clarified supernatant loaded onto a 5ml HisTrap HP nickel affinity column (GE Healthcare). dCry protein was eluted with 100 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using Ni-NTA affinity resin (Quiagen) and followed with SEC (Superose 6 Increase, GE Life Sciences), as previously described10 ...
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bioRxiv - Plant Biology 2020Quote: ... The secreted protein was purified from the supernatant by Ni2+ affinity chromatography on a HisTrap Excel column (GE healthcare), equilibrated in 50 mM KPi pH 7.6 ...
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bioRxiv - Microbiology 2020Quote: ... An additional affinity chromatography step was therefore included directly before gel filtration using 2 × 5 mL HisTraps (GE Healthcare). As both the MBP and TEV proteins incorporate a hexahistidine tag ...
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bioRxiv - Microbiology 2021Quote: ... as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Plant Biology 2021Quote: ... soluble proteins were purified by affinity chromatography using a HisTrap Ni-NTA column (ÄKTA pure FPLC system, GE Healthcare) equilibrated in 50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... The cleared supernatant was sterile filtered and loaded onto a 5 mL HisTrap HP Ni2+ affinity column (GE Healthcare). After washing with several column volumes of lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... And then affinity purified on GST-TbFUT1 fusion protein coupled to a HiTrap NHS-Activated HP column (GE Healthcare). Finally ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged GlpQ protein was purified by Ni2+-affinity chromatography using a 1 ml HisTrap column (GE Healthcare) followed by size exclusion chromatography on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Microbiology 2022Quote: ... as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).