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Citations for GE Life Sciences :
501 - 550 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... the samples were purified on nickel-affinity resin (Ni Sepharose High Performance; GE Healthcare, Little Chalfont, UK) after incubation for 30 min at 25 °C and centrifugation of the fraction bound to nickel to pull down the TgMIC-His that physically interacted with TLR-FLAG (16,000 g ...
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bioRxiv - Biophysics 2019Quote: ... purified by immobilized metal affinity chelate chromatography (IMAC) using a Ni2+ Sepharose 6 FastFlow column (GE Healthcare), and then labeled with maleimide-activated LD550 and LD650 fluorophores (Lumidyne Technologies ...
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bioRxiv - Immunology 2021Quote: ... Proteins were purified as above using Ni2+ affinity and size exclusion chromatography (Superdex 75, GE Life Sciences), excluding cleavage of the His6-tags.
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bioRxiv - Immunology 2021Quote: ... Affinity-purified SARS-CoV-2 RBD-SD1 was further purified over a Superdex75 column (GE Life Sciences). SARS-CoV-2 S HP ...
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bioRxiv - Biochemistry 2021Quote: AsChi18A was purified by immobilized metal affinity chromatography using a HisTrap FF 5 mL column (GE Healthcare). The periplasmic extract containing AsChi18A was applied on the column using a binding buffer consisting of 20 mM Tris-HCl pH 8.0 and 5 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was loaded into a 5 mL Ni–NTA affinity chromatography column (GE Healthcare, United States) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The cell lysate was centrifuged and the supernatant was incubated with Ni-NTA affinity resin (GE Healthcare). The target protein was eluted with lysis buffer supplemented with 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Biophysics 2020Quote: ... 100 mM NaCl pH 7.0 and further purified on a 5 ml Heparin affinity column (GE Healthcare) with a 100 ml gradient of 0.1 to 1.5 M NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 10% (v/v) glycerol) before loading on to a third affinity column (HiTrap Heparin HP, GE Healthcare). A step gradient (25-50-75-100% ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant 3CLpro were then purified using HIS Gravitrap Ni-NTA affinity columns (GE Healthcare, Chicago, IL), following the standard protocol (Kim et al. ...
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bioRxiv - Immunology 2021Quote: ... Rhesus sera or plasma antibodies were first purified by affinity chromatography using Protein A Sepharose (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Ni-affinity (HisTrap HP column) and anion exchange (MonoQ column) chromatography steps (all columns from GE Healthcare) as described previously 49 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were purified by immobilized metal-affinity chromatography (IMAC) using pre-packed nickel Sepharose columns (GE Healthcare), or in batch mode on TALON® beads (Takara Bio ...
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bioRxiv - Neuroscience 2022Quote: ... The Twin-Strep-tagged protein was purified via affinity chromatography using a StrepTrap HP column (GE Healthcare). Next ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was then subjected to first round of affinity purification with IgG-SepharoseTM (GE healthcare, Germany) for 1 hour at 4°C on the rotating wheel ...
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bioRxiv - Microbiology 2019Quote: ... The cleared lysate was loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare). His-tagged proteins were eluted with a linear gradient of buffer B (50 mM Hepes pH8 ...
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bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
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bioRxiv - Immunology 2020Quote: ... Affinity-purified SARS-CoV-2 RBD-SD1 was further purified over a Superdex75 column (GE Life Sciences). SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate was loaded on a Ni affinity step on HisTrap FF crude column (GE Healthcare, Inc.) and the protein was eluted at a concentration of 150 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble supernatants were purified by nickel-affinity chromatography (HiTrap Chelating HP column, GE Healthcare Life Science) using an NGC Quest 10 Plus Chromatography System (BIO-RAD) ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant loaded onto a freshly charged 5 mL HisTrap FF Ni-affinity column (GE Healthcare) at 1 mL/min at 4 °C (50 mM Tris buffer ...
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bioRxiv - Microbiology 2021Quote: ... The cleared lysate was subjected to a histidine affinity chromatography in a HisTrap HP column (GE Healthcare) equilibrated in Buffer A ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were purified by nickel affinity chromatography using HisTrap Column (GE Healthcare Life Sciences Pittsburgh, PA). Rat polyclonal antisera against L ...
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bioRxiv - Genomics 2021Quote: ... The eluates were then subjected to heparin affinity chromatography on a HiTrap Heparin HP column (GE Healthcare) using buffer A for binding (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... The soluble fraction was subjected to an affinity purification using a MBP FF crude column (GE Healthcare) for the KNL-2 fusion construct ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Affinity purified protein was further purified by size-exclusion chromatography (16/60 Superdex 75 PG, GE Healthcare) in the gel filtration buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... PETISCO was purified by immobilized metal affinity chromatography (IMAC) using Ni2+-chelating beads (HisTrap FF; GE Healthcare). The HisTrap FF column was washed with 20 column volumes of lysis buffer and proteins were eluted with 20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: Immobilized metal affinity chromatographic purification of PaPlaB was performed [34] using the ÄKTA Pure instrument (GE Healthcare). The Ni2+-NTA column (4 ml ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was purified via immobilized metal affinity chromatography (IMAC) on a HisTrapTMFF column (GE Healthcare) and eluted with a linear gradient to 40 mM HEPES pH 7.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Soluble supernatants were purified by affinity chromatography (StepTag or HiTrap Chelating HP columns, GE Healthcare Life Science) using an NGC Quest 10 Plus Chromatography System (BIO-RAD ...
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bioRxiv - Immunology 2020Quote: ... Affinity-purified SARS-CoV-2 RBD-SD1 was further purified over a Superdex75 column (GE Life Sciences). MERS-CoV S1 was purified over a Superdex200 Increase column (GE Life Sciences) ...
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bioRxiv - Microbiology 2021Quote: ... Secreted proteins were purified from the culture supernatants using a protein A+G affinity column (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... affinity chromatography with in-line dialysis in PBS via HiTrap 5 ml desalting columns (GE Healthcare – 29048684) using an Akta™ Pure system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to affinity purification with a His-Trap HP 5 mL (GE Healthcare) column ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded onto an immobilized metal-affinity column (1 mL, HIStrap HP column, GE Healthcare), which was washed with 50 mM sodium phosphate (NaPi ...
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bioRxiv - Immunology 2022Quote: ... MBs were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare, Chicago, IL) and eluted with 20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was loaded onto a Ni-NTA affinity chromatography column (HisTrap FF crude, GE Healthcare) equilibrated in Buffer A (50 mM Hepes pH8 ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Molecular Biology 2022Quote: ... and the supernatant was subjected to batch affinity purification with Glutathione Sepharose 4B Resin (GE Life Sciences). Bound protein was eluted in harvesting buffer containing GST-Precission protease ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).
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bioRxiv - Microbiology 2022Quote: ... the supernatant was loaded on a nickel affinity column (HiTrap™ Chelating HP 5 mL, GE Healthcare) previously equilibrated with 40 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: Proteins were purified by immobilized metal affinity chromatography (IMAC) using Ni-Sepharose excel resin (GE Healthcare, USA). The column was first washed with 5 column volumes of loading buffer (50 mM tris(hydroxymethyl)aminomethane (TRIS) ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were purified by nickel affinity chromatography using a 5 ml HisTrap HP column (GE Healthcare). The eluted proteins were dialyzed into 20 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... bNAb affinity columns were made by cross-linking IgG to activated CNBr-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified cell lysate was applied to a nickel affinity column (GE Healthcare; Sepharose high performance beads) equilibrated in Buffer A and purified by FPLC ...
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bioRxiv - Biochemistry 2023Quote: The obtained supernatant was incubated with pre-equilibrated Ni-Sepharose high performance affinity matrix (GE Healthcare Biosciences) in buffer A for 16 hours at 4 °C in a test tube rotor at 5 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was purified using nickel affinity chromatography with a 5 ml HisTrap HP column (GE Healthcare) followed by size exclusion chromatography on a HiLoad Superdex 75 16/600 column (GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... ISCU2 and FXN were subjected to Ni-NTA affinity chromatography (His-Trap 5ml FF crude; GE Healthcare). The column was washed with 10 CV IMAC wash buffer ...