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Citations for GE Life Sciences :
551 - 600 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: The amino acids 77-136 encoding the cytoplasmic region of the Hoka protein were cloned into pGEX-6P (GE Healthcare) to produce a GST (Glutathione S-transferase ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... Elution fractions containing receptor were pooled and subjected to polishing by SEC on a Superdex 200 Increase 10/300 GL column (GE Healthcare) in 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Immunology 2022Quote: ... assay was performed to quantify the binding avidity of the recombinant mos-tri-RBD to the receptor hACE2 using BIAcore 8K (GE Healthcare) with NTA chips ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Physiology 2020Quote: ... and a Multiphor II Electrophoresis System (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2021Quote: ... The column was calibrated using a set of gel filtration markers (low range, GE Healthcare), including Ovalbumin (43.0 kDa) ...
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bioRxiv - Cell Biology 2022Quote: ... cleared by low-speed centrifugation and filtered through a 0.45 µm filter (GE Healthcare Whatman) to yield “lentivirus supernatant” ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2020Quote: ... Fc-fused sdAbs were prepared with prepacked HiTrap® Protein A HP column (GE Healthcare). The produced Fc-fusion protein was analyzed by SDS-PAGE and the Western blot using standard protocols for dimerization ...
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bioRxiv - Microbiology 2021Quote: ... Fc-fused nanobody was prepared with prepacked HiTrap® Protein A HP column (GE Healthcare). The produced Fc-fusion protein was analyzed by SDS-PAGE using standard protocols for dimerization ...
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bioRxiv - Immunology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare) and cleaved using bovine enterokinase (GenScript ...
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bioRxiv - Microbiology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare). The protein was cleaved using bovine enterokinase (GenScript ...
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bioRxiv - Cell Biology 2021Quote: ... 32°C or 42°C using a DeltaVision imaging system (Applied Precision Ltd.) composed of an IX-inverted widefield microscope (Olympus ...
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bioRxiv - Cell Biology 2020Quote: ... 32°C or 37°C using a DeltaVision imaging system (Applied Precision Ltd.) composed of an IX-inverted widefield microscope (Olympus ...
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bioRxiv - Cell Biology 2020Quote: ... The peak fractions were pooled and run on a nickel affinity column (HisTrap FF crude column, GE Healthcare) equilibrated with septin 2 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Microbiology 2019Quote: ... and O-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant rhodopsin was purified by affinity chromatography (HisTrap™ FF Crude column, GE Healthcare Life Science, Chicago, IL) and gel filtration (HiPrep™ 26/10 desalting ...
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bioRxiv - Microbiology 2019Quote: ... Anti-CFP polyclonal antibodies were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare) according to manufacturer instructions.
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bioRxiv - Cell Biology 2019Quote: Protein purification was performed by immobilized metal-affinity chromatography (IMAC) on HisTrap HP Ni2+ Sepharose columns (GE Healthcare) at 4°C according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The His6-tagged phytochrome samples were first purified with NiNTA affinity purification using HisTrap™ columns (GE Healthcare), followed by size-exclusion chromatography (HiLoad™ 26/600 Superdex™ 200 pg ...
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bioRxiv - Bioengineering 2021Quote: ... The protein was purified through nickel-agarose affinity chromatography followed by gel filtration chromatography (S-300, GE Healthcare). The purified protein was concentrated to 30 mg/mL in a buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Bioengineering 2020Quote: ... affinity column followed by size exclusion chromatography purification with a Superdex 200 10/300 increased column (GE Healthcare). Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: The His-tag affinity-purified GINKO1 protein was further applied on the size exclusion chromatography Superdex200 (GE Healthcare) column pre-equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... pastoris X-33 and the secreted proteins were purified by affinity chromatography (HiTrap Chelating HP column) (GE Healthcare). Eluted proteins were copper saturated with Cu(II)SO4 and applied to a 120 mL Superdex 75/16 column (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... supplemented with 20 mM imidazole and loaded onto a 5 mL HisTrap HP Ni2+ affinity column (GE Healthcare). After washing with several column volumes (CV ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was incubated with GFP nanobody-conjugated affinity resin (CNBr-activated Sepharose 4B resin from GE Healthcare) by rotating at 4°C for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... the medium was loaded overnight onto a ~5 ml anti-CD9 AR40A746.2.3 affinity column (7.5 mg antibody/ml CNBr Activated Sepharose 4B beads; GE Healthcare) in line of an AKTA explorer (GE) ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D534 was also purified using 1 mL of M2 anti-FLAG affinity column (GE healthcare) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2020Quote: Detergent-solubilized TmrAB was next purified by loading onto a Nickel affinity column (HisTrap IMAC HP, GE Healthcare) and washing with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... Enzymes were isolated from the cleared lysates via nickel affinity chromatography (IMAC) using a HisTrap column (GE Healthcare) and size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was purified by affinity chromatography on the Glutathione-Sepharose 4B beads (GE Healthcare, GE17-0756-05) equilibrated in GST-A buffer ...
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bioRxiv - Immunology 2022Quote: ... 5% glucose) and the proteins purified by affinity chromatography using a HiTrap Heparin High-Performance column (GE Healthcare) and gradient elution (0-100% NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL Glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... and the supernatant applied to Ni-NTA agarose followed by glutathione-agarose affinity column (GSTrap HP, GE Healthcare). AP-2-containing fractions were pooled ...
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bioRxiv - Plant Biology 2019Quote: ... GST-tagged and His-tagged proteins were affinity-purified using glutathione agarose beads (GE Healthcare, Pittsburgh, PA, USA) and Ni-NTA affinity agarose beads (QIAGEN ...
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bioRxiv - Cancer Biology 2019Quote: ... immunoblot membranes were probed with HRP-conjugated affinity-purified donkey anti-mouse or anti-rabbit IgG (GE Healthcare) as secondary antibodies and visualized with the immobilon Western Chemiluminescent HRP substrate (Millipore).
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was applied to a 5ml IMAC (Immobilized Metal-Ion-Affinity Chromatography) HiTrap Chelating column (GE Healthcare) preloaded with 100 mM zinc sulphate and equilibrated with low IMAC1 buffer (50 mM Na-Phosphate buffer ...
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bioRxiv - Biophysics 2019Quote: ... The solubilized fraction was subjected to affinity purification by 1D4 antibody conjugated to CNBr-activated agarose (GE Healthcare). The resin was extensively washed and the protein eluted with wash buffer supplemented with 0.2 mg/ml 1D4 peptide ...
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bioRxiv - Molecular Biology 2020Quote: ... 12 μl of pre-blocked m7G affinity beads (50 % slurry, 7-Methyl-GTP Sepharose® 4B, GE Healthcare) and 200 μl of BB-250 (250 mM NaCl ...
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bioRxiv - Biochemistry 2019Quote: ... mTLR9-ECD was purified by protein A-affinity chromatography with IgG Sepharose 6 Fast Flow resin (GE Healthcare), washed with PBS and eluted with 0.1 M glycine-HCl pH 3.5 ...
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bioRxiv - Microbiology 2021Quote: Antibody affinities were determined by SPR essentially as described44 using a Biacore 8K instrument (GE Healthcare, Chicago, IL). To measure antibody interaction affinity rather than avidity ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested and purified by Ni-NTA affinity chromatography and gel filtration (Superdex 200, GE Healthcare, USA) and eluted in PBS (pH 7.2) ...
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bioRxiv - Physiology 2019Quote: ... The vector was purified from the clarified lysate by affinity chromatography over a HiTrap heparin column (GE Healthcare), and ultracentrifuged overnight before resuspension in sterile physiological Ringer’s solution ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble fraction was subjected to an affinity purification using a chelating HiTrap FF crude column (GE Healthcare) charged with Ni2+ ions on an AKTA-HPLC purifier (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: The affinity of nanobodies was measured by surface plasmon resonance (Biacore 3000, GE Healthcare Bio-Sciences AB, Sweden). Recombinant RBD protein at a concentration 20 μg/ml in 10 mM acetate buffer (pH 4.5 ...