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Citations for GE Life Sciences :
801 - 850 of 2372 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and purified over a HisTrap FF nickel affinity column followed by Superdex 75 size exclusion chromatography using the ÄKTA protein purifier purification system (GE Healthcare), as previously described (27) ...
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bioRxiv - Biochemistry 2020Quote: ... The expressed soluble proteins were purified by affinity chromatography using a 5 ml prepacked HisTrap HP column on an AKTA Pure protein purification system (GE Healthcare). The fractions were further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2021Quote: The binding affinity of the discovered antibodies for α-cobratoxin was determined using Surface Plasmon Resonance (SPR) (BIAcore T100, GE Healthcare). All measurements were performed at 25 °C using 10 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... Each recombinant segment was purified by Ni2+-affinity chromatography using HisTrap HP purification columns with a FPLC system (ÄKTA Purifier, GE Healthcare) with elution buffer consisting of 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Molecular Biology 2020Quote: Purified Affimer proteins were incubated with KRAS lysates overnight at 4°C and the complexes purified by Ni-NTA affinity chromatography and size exclusion chromatography using HiPrep 16/60 Sephacryl S-100 column (GE Healthcare). The complexes were concentrated to 24 mg/ml (KRAS:K3 ...
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bioRxiv - Biophysics 2022Quote: ... Cell disruption was achieved by sonication (Branson Sonifier SFX250) and the soluble fraction was subjected to immobilized metal affinity purification using a 5 ml Ni-NTA column (GE healthcare) connected to a FPLC system (GE healthcare) ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate was clarified by centrifugation at 35,000g and purified by immobilized metal affinity chromatography (IMAC) on a 5 mL Fast Flow Chelating Sepharose column (GE Life Sciences) with an elution gradient from 0 to 200 mM imidazole over 120 mL ...
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bioRxiv - Cell Biology 2019Quote: ... as described previously for the non-fluorescent version (39): i) affinity purification of filtered supernatant with 5 ml HisTrap FF column (GE Healthcare) and step elution with 300 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... and purified using a standard step gradient (300 mM imidazol) immobilized metal affinity chromatography (IMAC) protocol (Ni-Sepharose Fast Flow, GE Healthcare). Afterwards ...
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bioRxiv - Biophysics 2019Quote: ... 5 mg/mL of purified rPrP90-231 fused to an N-terminal six-histidine tag was immobilized in a nickel-loaded HisTrap HP affinity column (GE Healthcare). DNA library was added to the resin ...
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bioRxiv - Biophysics 2019Quote: ... Rab8-His6 proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Unspecifically bound proteins were removed by a step wash with 5% buffer B (50 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Supernatant was filtered and N-glycosylated proteins isolated by affinity chromatography using Concavaline A columns (HiTrap Con A 4B, GE Healthcare) in AKTA FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... it was purified on a nickel affinity chromatography and finally subjected to size exclusion chromatography on a Superdex 200 10/300 gel filtration column (GE healthcare) in 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... All affinity-purified proteins were size-exclusion-purified using a Superdex 200 Increase 10/300 GL column (GE Healthcare, catalog 28990944) in HEPES buffered saline (50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was further purified using reverse affinity chromatography on Ni-Sepharose followed by gel filtration (Superdex 200 16/60, GE Healthcare). Protein in gel filtration buffer (10 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... and trimeric complexes were affinity purified via 6xHis-tagged RING1B on Ni2+-charged IMAC Sepharose 6 Fast Flow resin (GE Healthcare). 10 mM imidazole was added to lysates during binding ...
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bioRxiv - Biochemistry 2020Quote: ... All proteins were isolated from the medium exploiting the affinity of the 6xHis tag for the HisTrap Excel (GE Healthcare, USA) affinity column ...
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bioRxiv - Physiology 2019Quote: ... which was purified via sequential protein-A affinity and size exclusion chromatography (SEC) techniques using MabSelect SuRe and Superdex 200 purification media respectively (GE Healthcare), as described elsewhere (Suriben et al ...
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bioRxiv - Physiology 2019Quote: ... and purified from conditioned media by sequential protein-A affinity and size-exclusion chromatographic (SEC) methods using MabSelect SuRe and Superdex 200 purification media respectively (GE Healthcare). All purified antibody material was verified endotoxin-free and formulated in PBS for in vitro and in vivo studies ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... Kinetic parameters and affinities of protein-protein interactions were measured by surface plasmon resonance (SPR) analysis using a Biacore T100 biosensor (GE Healthcare). 2000 response units (RU ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were centrifuged at 18000 rpm (F21-8×50y rotor) for 45 min and supernatants were purified by Ni2+-affinity chromatography (FPLC system ÄKTA Purifier, GE Healthcare) eluting the protein in 50 mM NaPO4 ...
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bioRxiv - Biophysics 2020Quote: ... which was performed by batch immobilized metal ion affinity (IMAC) using nickel agarose slurry (Ni-NTA, QIAGEN or Ni Sepharose High Performance, GE Healthcare) and size-exclusion chromatography (SEC ...
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bioRxiv - Bioengineering 2020Quote: ... and purified via Ni-NTA affinity chromatography followed by size exclusion on a HiLoad 16/600 Superdex 75 column (GE Healthcare) in PBS buffer.
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bioRxiv - Biochemistry 2020Quote: ... the supernatants were collected for a three-step purification by nickel-nitrilotriacetic acid affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
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bioRxiv - Biochemistry 2020Quote: IgGs were produced by co-transfection of the heavy chain and light chain pEU vectors (76) in mammalian cells and the IgG1 variants were purified by protein A affinity chromatography (MabSelect Sure columns, GE healthcare) using the AKTA express system and eluted with 0.1 M sodium citrate (pH 3.0) ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction was incubated with GST-CDK2 and then purified by affinity chromatography using Sepharose 4B GST resin beads (GE Healthcare) and subsequently eluted in 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Purification of enzyme having N-terminal hexahistidine tag was done by Ni-NTA affinity chromatography using an Akta pure FPLC system (GE Healthcare). Equilibration buffer was 50 mM tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were affinity purified on an amylose column and were further purified by ion-exchange chromatography (HiTrap Q HP, GE Healthcare). Protein concentrations were determined by UV spectroscopy ...
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bioRxiv - Biochemistry 2020Quote: ... The full length hTop2α was first purified by a tandem affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE healthcare) bound to Ni2+ ions followed by a StrepTrap HP column (GE healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... and the supernatant was loaded onto an equilibrated (lysis buffer) immobilised metal affinity chromatography column (IMAC) (Ni-IDA Sepharose, GE healthcare).
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were dialyzed into 20 mM sodium phosphate pH 7.4 with 500 mM sodium chloride and the recombinant RBD was purified by immobilized metal ion affinity chromatography (IMAC) using a HisTrap excel column (GE Healthcare). The purified protein was biotinylated using the E ...
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bioRxiv - Plant Biology 2020Quote: ... Cleaved proteins were subsequently purified from the protease and affinity tag by a second Ni2+-affinity column or by gel filtration on a Superdex 200 Increase 10/300 GL column (GE Healthcare). Proteins were concentrated to 3–10 mg/ml and either used immediately or aliquoted and quickly frozen at –80°C ...
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bioRxiv - Microbiology 2021Quote: ... The His-tag itself and traces of uncleaved protein were subsequently removed by nickel affinity chromatography on 1 ml Ni-Sepharose 6 FF resin (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Microbiology 2021Quote: ... and the secreted proteins were purified from supernatant by nickel affinity chromatography using Ni-NTA IMAC Sepharose 6 Fast Flow resin (GE Healthcare) followed by size exclusion chromatography on a Superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Soluble CV-B3 Δ116-2C was purified from the supernatant by immobilized metal-affinity chromatography (IMAC) on a HisTrap column (GE Healthcare) and eluted with a buffer consisting of 50 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... Eluates of immobilized metal ion affinity chromatography (IMAC) were diluted to 150 mM NaCl and loaded onto a HiTrap Heparin HP column (GE Healthcare). Protein complexes were eluted by a linear salt gradient from 150 mM to 2 M NaCl ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was passed over a GST-affinity column (glutathione sepharose™ 4B beads GE Healthcare, Little Chalfont, Buckinghamshire, UK) three times at least ...
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bioRxiv - Microbiology 2020Quote: ... Refolded protein was filtered through a 0.45 μm filter prior to immobilised metal affinity chromatography (IMAC) using a HisTRAP™ High Performance column (GE Healthcare), which was equilibrated with IMAC buffer A.
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bioRxiv - Molecular Biology 2021Quote: ... All proteins were first purified using affinity chromatography on HisTrap column followed by HiTrap heparin purification in 250-1500 mM gradient of NaCl (GE Healthcare). Protein concentration was determined by the Pierce BCA reagents (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... assay was performed to determine the binding affinities of aCPSF1 to poly-U-tract containing RNA on a BIAcore 8K instrument (GE Healthcare) as described previously (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... After cell sonication proteins were affinity purified using a Glutathione sepharose 4b or remains on sepharose for pull down (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Immobilized Metal Affinity Chromatography (IMAC) was performed for protein purification using an AKTA Start Chromatography system (GE Healthcare Bio-Science, Sweden). The soluble fraction containing TNFα was diluted (1:1 ...
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bioRxiv - Molecular Biology 2021Quote: ... and expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Microbiology 2020Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... The affinity-purified protein was further purified by size exclusion chromatography on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) in HPLC buffer (20 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... Fusion proteins were recovered from the cell lysates by conventional affinity chromatography on His-trapTMHP columns (GE Healthcare, 17-5247-01) using an Akta Explorer system (Amersham Pharm BiotechGE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were subsequently purified via affinity chromatography using HisTrap or MBPtrap columns connected to an ÄKTA Explorer liquid chromatography system (GE Healthcare) according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal thioredxin-His6-tagged proteins were purified using Ni-NTA agarose affinity column followed by size-exclusion chromatography (Superdex 200 column, GE Healthcare) in the buffer containing 50 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...