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Citations for GE Life Sciences :
701 - 750 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... the supernatant of stable HEK293S cells expressing αMβ2 headpiece was recovered and purified by immobilized ion-affinity chromatography using a 5 mL HisTrap Excel column (GE Healthcare). The protein was subsequently applied to a 1 mL StrepTactin column (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... Purification was performed via batch affinity chromatography using glutathione agarose 4B (Macherey and Nagel) or glutathione sepharose 4B (GE Healthcare) in the same buffer ...
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bioRxiv - Biochemistry 2019Quote: ... The template strand was purified by binding the biotinylated PCR product to Streptavidin Sepharose High Performance affinity resin (GE Healthcare), purification of the beads from the reaction mixture by repeated washing with Tris buffer (10 mM Tris HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... the protein of interest was enriched via metal ion affinity chromatography (Ni-NTA) resin (IMAC, 5ml HiTrap Chelating, GE Healthcare). After 10 column washes with (20 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... The His6-tag-containing FdR9 was purified by affinity chromatography on an Äkta prime FPLC system (GE Healthcare, Freiburg, Germany), using a 5 mL HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... All parkin constructs were purified by Ni2+-NTA affinity chromatography by HisTrap FF column on an AKTA FPLC (GE Healthcare) and the affinity tag was cleaved by Ulp1 protease (1:50 protease:protein) ...
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bioRxiv - Biophysics 2021Quote: ... nickel affinity chromatography was performed where the lysate was loaded onto a 5 mL HisTrap™ Excel column (GE Healthcare), followed by a wash with 20 column volumes (CV ...
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bioRxiv - Biophysics 2020Quote: ... Supernant containing soluble proteins was filtered using a 0.45 μm device and applied to a HisTrap HP affinity column (GE Healthcare) equilibrated in buffer A (40 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were expressed in Rosetta 2 bacteria and purified by affinity chromatography using a HiTrap™ chelating column (GE Healthcare) according to the manufacturer’s instructions in 50 mM Tris at pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant was filtered through tandem 1-μm and 0.45-μm filters before Ni2+-affinity purification (HisTrap excel, GE Healthcare). Ni2+-bound proteins were washed with buffer A and eluted directly onto a coupled Strep-Tactin Superflow XT column (IBA ...
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare), conjugated to TaBGC1 recombinant protein.
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bioRxiv - Microbiology 2021Quote: ... Anti-Fh15 polyclonal IgG was purified by affinity chromatography using 5/5/HiTrap Protein-A HP (GE Healthcare, Piscataway, NJ). The resulting IgG was conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Plant Biology 2021Quote: ... The proteins were then purified by affinity chromatography using Ni-NTA agarose resin and Glutathione Sepharose 4B resin (GE Healthcare), respectively.
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was subjected to immobilized-metal affinity chromatography (IMAC) using a 5 mL HisTrap HP column (GE Healthcare) equilibrated with buffer ParB1 containing 20 mM imidazole ...
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bioRxiv - Microbiology 2020Quote: ... Affinity-purified ComGCSS was further purified by gel-filtration chromatography on an HiLoad 16/600 Superdex 75 column (GE Healthcare), using (25 mM Na2HPO4/NaH2PO4 pH 6 ...
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bioRxiv - Microbiology 2020Quote: ... Biovar-specific LPS antibodies were purified by standard affinity chromatography as described by the manufacturer (GE Life Sciences, PA USA).
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bioRxiv - Molecular Biology 2021Quote: ... Trimer-containing fractions were pooled and passed over a pre-equilibrated MAbSelect SuRe Protein A (ProA) affinity column (GE Healthcare) to remove leached residual PGT145-affinity ligand from the previous column ...
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bioRxiv - Microbiology 2020Quote: Recombinant C-terminal GST-tagged hA3C and hA3C.WE-RK were expressed in HEK293T cells and purified by affinity chromatography using Glutathione Sepharose 4B beads (GE Healthcare) as described previously [65] ...
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bioRxiv - Cell Biology 2021Quote: ... His-Bro1ΔAPRR purified from BL21-DE3 using Ni2+-affinity chromatography (5 ml HiTrap Chelating FF column; GE Healthcare, United Kingdom). GST-Bro1V(370-709 ...
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bioRxiv - Cell Biology 2020Quote: ... The protein purification was done through affinity chromatography on a Ni-column (HiTrap chelating HP column 1 ml, GE HealthCare). The column was loaded with a filtered solution of 100 mM nickel chloride ...
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bioRxiv - Biophysics 2020Quote: ... Recombinant eGFP-GST-tagged PH- domain of Grp1 detecting PI(3,4,5)P3 was expressed overnight at 18 ºC using 1 mM IPTG in Escherichia coli strain BL21(DE3) and purified by affinity chromatography using glutathione Sepharose 4B beads according to the manufacturer’s instructions (GE Healthcare) in 50mM Tris at pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... 0.05% NP-40) and assembled complexes were affinity purified from the cushions using lgG Sepharose 6 Fast Flow (GE Healthcare) to capture the Protein A tag ...
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bioRxiv - Cell Biology 2021Quote: ... The kinase complex was isolated using IgG sepharose 6 Fast Flow and Calmodulin sepharose 4B affinity resin (both GE Healthcare). In the last step of all bacterially expressed proteins ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM TCEP) and the sample was loaded onto a 5 mL HiTrap Heparin HP affinity column (GE healthcare) pre-equilibrated with 5% buffer B (50 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was consecutively filtered through 0.45 µm and 0.22 µm PES syringe filters prior to loading onto a 5 ml HisTrap nickel affinity column (GE Healthcare) preequilibrated with Buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... the affinity-purified Pvs25 protein was applied to hydrophobic interaction chromatography (HIC) (HiTrap Phenyl HP; 5 mL resin, GE Healthcare) In detail ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate of HIC-EBA was subjected to affinity chromatography using Strep-Tactin® column (5 ml cartridge, GE Healthcare Life Science ...
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bioRxiv - Plant Biology 2022Quote: ... strain BL21 as described previously,20 using the GST gene fusion system for heterologous expression and affinity purification (GE Healthcare). DNA templates for the cystatins were synthesized as g-blocks (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: ... and the supernatant was filtered using a 0.2 μl filter and flown over a 5 ml NiNTA Immobilised Metal Affinity Chromatography (IMAC) column (GE Healthcare) equilibrated with buffer A (25 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysate was centrifuged at 45,000 x g for 35 minutes and supernatant was loaded onto a HisTrap HP affinity column (GE Healthcare). A linear gradient of buffer B (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... and passed through a 0.45-μm filter prior to protein purification via affinity chromatography using an AKTA Start FPLC system (GE Healthcare). The lysate was loaded into a HisTrap HP 5 mL column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... The lysate was centrifuged and the supernatant was filtered through a 0.45 μm filter and subjected to a His-tag affinity chromatography using a 5 mL HisTrapFF (GE Healthcare) and the ÄKTAprime plus system (GE Healthcare) ...
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bioRxiv - Biophysics 2022Quote: ... Protein eluted from the affinity columns was further purified by size-exclusion chromatography using a Superdex 75 column (GE healthcare).
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bioRxiv - Cancer Biology 2022Quote: ... Rac1 protein went through a nickel affinity column followed by a Resource Q column and finally Superdex 75 (GE Healthcare) before concentration to 25mg/ml ...
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bioRxiv - Microbiology 2020Quote: ... Antibody supernatants were harvested seven days following transfection and purified using protein G affinity chromatography following the manufacturer’s protocol (GE healthcare). In order to validate the functional relevance of Arg100 ...
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bioRxiv - Microbiology 2021Quote: ... The cleared extract was filtered (0.45 μm) before loading onto a nickel-nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated with lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... Background binding to blank immobilized flow cells was subtracted and affinity constants were calculated using BIA Evaluation software (GE Healthcare) using the Steady State Affinity model ...
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bioRxiv - Immunology 2020Quote: The binding kinetics and affinity of mAbs to SARS-CoV-2 RBD were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was filtered using a 0.2 μM filter and loaded onto a 5 ml HisTrap HP Ni-affinity column (GE Healthcare). Protein was eluted in a gradient of 10 to 300 mM imidazole over five column volumes ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were pelleted and lysed by sonication in lysis buffer (Tris 100mM, NaCl 0.5M, glycerol 5%) and eGFP was purified using fast flow His-affinity columns (GE Healthcare) and eluted with 500 mM imidazole ...
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bioRxiv - Immunology 2020Quote: ... Antigen-bearing components were purified using immuno-affinity column with immobilized PGT145 IgG (Sepharose 4B resin, GE Healthcare Life Sciences). 3 M MgCl2 + 250 mM l-arginine (pH 7.2 ...
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bioRxiv - Neuroscience 2021Quote: ... After centrifugation at 60,000 × g the supernatant was used for purification using nickel-affinity chromatography on a HisTrap HP column (GE Healthcare), using an isocratic elution of low to high imidazole buffer (500 mM imidazole ...
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bioRxiv - Genetics 2019Quote: ... Lysate was clarified by centrifugation at 25,000xg and soluble protein was purified using Co2+ affinity chromatography (TALON column, GE Healthcare) followed by anion-exchange chromatography (Q-FF ...
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bioRxiv - Cell Biology 2019Quote: ... The protein was purified via a two-step protocol of Ni-affinity using a 1ml His-Trap column (GE Healthcare) in Ni-affinity buffer [50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 6xHis tagged protein was purified by IMAC affinity purification using 0.5 ml of packed Sepharose 6 Fast Flow Ni-NTA Resin (GE Healthcare) pre-washed with lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... the myosin was purified by His-tag affinity purification with a 1 ml HisTrap HP nickel-sepharose column (GE Healthcare). The eluted myosin solution was then dialyzed overnight at 4 °C in a low-salt buffer (25 mM imidazole pH 7.0 ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant RADA and RADA[K201A] proteins were affinity purified in a precalibrated HisTrap FF Crude (GE Healthcare Life Sciences) column run at 0.5 mL/min ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was filtered through a 0.22 μm syringe filter and loaded onto an immobilized Ni2+ affinity column (HisTrap HP 5ml, GE Healthcare) using buffer A and eluted using a gradient of 10-500 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were spun down at 12,000 g for 30 min and the supernatants were loaded onto a His-Select Nickel Affinity gel (GE Healthcare). Recombinant PstS proteins were eluted with the elution buffer (500 mM imidazole ...
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bioRxiv - Neuroscience 2021Quote: ... Viral particles were sequentially purified on iodixanol (Axis-shield, Dundee, United Kingdom) and ion-exchange affinity columns (GE Healthcare, Italy). Viral genomic copies were measured by TaqMan quantitative PCR (Invitrogen ...