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Citations for GE Life Sciences :
401 - 450 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Hexa-histidine tagged protein was purified using Nickle-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta prime FPLC system ...
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bioRxiv - Molecular Biology 2020Quote: ... and protease inhibitors) was then passed over a glutathione affinity column (Sepharose 4B, GE Healthcare), washed with buffer (40 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Expressed S2Pecto protein was isolated by metal affinity chromatography on HisTrap Excel columns (GE Healthcare), followed by further purification on a StrepTrap HP column (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... Immobilized metal affinity chromatography (IMAC) using His-Trap FF Ni+2-NTA column (GE Healthcare) attached to BioRad™ NGC system ...
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bioRxiv - Immunology 2019Quote: ... The supernatant was then applied to a nickel-affinity chromatography (His Trap excel, GE Healthcare) equilibrated with buffer B (20mM Tris pH 7.2 ...
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bioRxiv - Biophysics 2019Quote: ... Clarified lysates were purified by affinity purification (GSTrap or HisTrap, GE HEALTHCARE, Little Chalfont, UK) and purity was assessed by SDS-PAGE ...
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bioRxiv - Microbiology 2019Quote: ... by immobilized metal ion affinity chromatography (IMAC) using HisTrap FF column (GE Healthcare Buckinghamshire, UK), in denaturing condition ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was applied to a 5-ml HisTrap HP nickel-affinity column (GE Healthcare) preequilibrated in wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: Binding affinity of a repebody was determined through surface plasmon resonance (Biacore T200, GE Healthcare). Briefly ...
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bioRxiv - Immunology 2020Quote: ... the His-tagged proteins were purified using nickel-affinity chromatography (HiTrap chelating, HP; GE Healthcare) with an imidazole gradient (10-250 mM ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was then passed through a HisTrap FF Ni2+-NTA affinity column (GE Healthcare) that had been equilibrated with 10 column volumes of equilibration buffer (75 mM KxHxPO4 ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was used for affinity purification and incubated with Glutathione Sepharose 4B (GE healthcare). The beads were washed in wash buffer (100 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto Ni–NTA Sepharose High Performance affinity resin (GE Healthcare Biosciences) pre-equilibrated with buffer A ...
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bioRxiv - Cell Biology 2020Quote: ... Sec23/24 was affinity purified following application to a 5 mL HisTrap column (GE Healthcare) equilibrated in lysis/binding buffer (20 mM HEPES (pH 8.0) ...
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bioRxiv - Molecular Biology 2020Quote: ... The His-tagged proteins were purified by cobalt affinity chromatography using Chelating Sepharose (GE Healthcare). The proteins were eluted in buffer containing 25 mM Hepes-KOH (pH 7.4) ...
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bioRxiv - Microbiology 2021Quote: The enzyme was purified by nickel affinity chromatography using HisTrap™ HP column (GE Healthcare). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Affinity constants (KD) were then calculated using BIAcore T200 evaluation software v.2.0 (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column, GE Healthcare) and SEC (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Plant Biology 2022Quote: ... pastoris culture medium was achieved by affinity chromatography on 5 ml HisTrapFF column (GE Healthcare; equilibrated in 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... onto a pre-equilibrated 5 mL nickel nitrilotriacetic acid affinity column (HisTrap HP, GE Healthcare) with a running buffer (20 mM Tris buffer at pH 7.5 containing 200 mM NaCl and 30 mM imidazole) ...
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bioRxiv - Biochemistry 2023Quote: ... StrepII-tagged Cas1-Cas2/3 complexes were affinity purified on StrepTrap HP resin (GE Healthcare) and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: ... FhuF was then purified from the supernatant using a His-trap affinity column (GE Healthcare) where the fraction containing FhuF was eluted at 20 mM Potassium Phosphate pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was isolated by affinity binding to Glutathione Sepharose® 4B resin (GE Healthcare). GST tag was then cleaved off with GST-HRV 3C protease ...
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bioRxiv - Microbiology 2023Quote: ... The soluble fraction was loaded onto a StrepTap HP 1 mL affinity column (GE Healthcare) at 4°C previously equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 and affinity-purified with GST beads (#17-0756-01, GE Healthcare Life Sciences). 293T cells overexpressing indicated proteins were treated with DNA damage reagents and lysed ...
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bioRxiv - Immunology 2023Quote: ... the filtered supernatant ran through a protein A and protein G affinity column (GE Healthcare) and eluted with 0.2 M citric acid at pH 3.0 and neutralized in 2 M Tris-base ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were purified by nickel affinity chromatography using Ni Sepharose 6 Fast Flow (GE Healthcare) and eluted with 20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biophysics 2024Quote: The affinity of RCC1 for nucleosome was measured on a Biacore 8K biosensor (GE Healthcare) by surface plasmon resonance (SPR) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the his-tagged FABP1 was purified using HisTrap HP affinity column (GE Healthcare, Chicago, IL), the tag was cleaved by thrombin and the cleaved protein was purified and buffer exchanged by gel filtration into 10 mM potassium phosphate pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Nickel affinity chromatography columns (His-Trap HP columns, GE Healthcare, Chalfont St Giles, Bucks., UK) were used to purify the polyhistidine-tagged roGFP2-GPX fusion proteins with a gradient elution (50 mM Tris ...
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bioRxiv - Microbiology 2019Quote: ... Viral DNA was extracted from 500-μl subsamples using the Zymo Research Viral DNA kit following the manufacturer’s protocol and subsequently amplified isothermally at 30°C using Genomiphi Whole Genome Amplification Kit (GE Healthcare, Little Chalfont, UK). Samples were cleaned and concentrated using a Zymo Research DNA Clean and Concentrator kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved receptor was separated from eGFP by SEC on a Superdex 200 10/300 GL column (GE Healthcare) pre-equilibrated with m7-glycerol+ buffer containing 6.7 mM DMCH ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% fetal calf serum (FCS; GE Healthcare Bio-Sciences, Chicago, IL), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were used for silverstaining (PageSilver kit, Fermentas) or transfer to Hybond-C nitrocellulose mebranes (GE Healthcare). After overnight blocking in PBS with 5% nonfat dry milk and 0.1% Tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... PirA-His6 from the supernatant was purified by Ni-affinity chromatography using HisTrapHP column (GE Healthcare) and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was first purified by affinity chromatography using Glutathione Sepharose 4B resin (GE Healthcare) and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich ...
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bioRxiv - Immunology 2019Quote: ... reaction mixture was applied to a 1 mL HiTrap KappaSelect affinity column (GE Healthcare Life Sciences). All affinity purification steps were carried out by connecting the affinity column to a peristaltic pump with flow rate of 1ml/min ...
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bioRxiv - Molecular Biology 2020Quote: ... For Brat-NHL serial reverse affinity and heparin chromatography (HiTrap HP Heparin 5ml column – GE Healthcare) followed the affinity chromatography ...
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bioRxiv - Bioengineering 2022Quote: ... with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow, 5 mL, GE Healthcare). Samples were eluted with imidazole ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Biochemistry 2019Quote: ... and the supernatant was loaded onto a Ni2+ affinity column (HisTrap HP 5 ml, GE Healthcare). The column was washed with 5 column volumes (CV ...
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bioRxiv - Developmental Biology 2019Quote: ... His6-SUMO-tagged proteins were purified by affinity chromatography using a HisTrap HP column (GE Healthcare), followed by the cleavage of the His6-SUMO tag using Ulp1 protease ...
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bioRxiv - Immunology 2021Quote: ... and the supernatant was loaded to a HisTrap Chelating HP affinity column (GE Healthcare Life Sciences) previously equilibrated with 50 mM MOPS pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare). The protein was dialyzed against 1X PBS overnight in the presence of 1:1000 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare) in 1XPBS pH 7.5 and eluted with 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The cleared supernatant was loaded on a 5 ml HisTrap FF nickel affinity column (GE Healthcare), pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: Binding affinities were measured by isothermal titration calorimetry (ITC) using a MicroCal iTC200 system (GE Healthcare) at 25°C using wild type truncated SPPV14 as well as two mutants SPPV14 Y46A and R84A in 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... The protein was isolated by affinity chromatography (5 ml HisTrap, GE Healthcare Life Science, Chicago, USA) and desalted (5 ml HiTrap ...