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Citations for GE Life Sciences :
851 - 900 of 2313 citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: The affinity between H2 and A33 was measured at room temperature using a Biacore T100 system with CM5 chips (GE Healthcare) according to the manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... TEV and residual affinity tag was removed by passing the protein through a Superdex 200 Increase 10/300 GL (GE Healthcare) in SEC buffer (30 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The GyrA protein was purified by nickel-affinity chromatography on a manually packed XK 26/20 column (Pharmacia) with Chelating Sepharose 6 Fast Flow resin (GE Healthcare) bound to Ni2+ ions ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant protein in the soluble cell fraction was purified by immobilized metal affinity chromatography (IMAC) using a HiTrap Chelating HP 1-ml column (GE Healthcare) with an ÄKTA purifier FPLC system (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... with expression induced with 0.5 mM IPTG for 18 h at 18°C and purified as N-terminal His6-tag or N-terminal His6-GST tag fusion proteins by affinity chromatography and size exclusion chromatography using a HiLoad 16/600 Superdex 200 column (GE Healthcare) equilibrated in 50 mM Tris/HCl ...
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bioRxiv - Cell Biology 2019Quote: ... The poly-histidine-tagged G3BP-1-WT as well as the mutated S149A and S149E constructs were purified using immobilized metal affinity (IMAC, HisTrap FF, GE Healthcare) and the dimer was isolated using size exclusion chromatography on a Superdex 75 column (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... Pnf was purified over HisTrap™ Ni2+-affinity column with the fast phase liquid chromatography (FPLC) AKTA system as per the manufacturer’s protocol (GE Healthcare). Plasmids pGEX-2T-wtRhoA ...
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bioRxiv - Microbiology 2019Quote: ... Rac1 and Cdc42 were purified over GSTrap HP™ affinity columns with the FPLC AKTA system as per the manufacturer’s protocol (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... were expressed in BL21(DE3) and then affinity-purified using glutathione Sepharose 4B beads (ABT) and HisTrap HP column (GE Healthcare), respectively ...
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bioRxiv - Biochemistry 2019Quote: ... SapB-H6 was purified from conditioned media by nickel affinity and size exclusion chromatography with a Superdex 75 column (GE Healthcare) in cross-linking buffer ...
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bioRxiv - Biochemistry 2019Quote: ... The final size-exclusion chromatography after affinity purification and tag removal was performed using Superdex75 16/60 HiLoad and Superdex75 increase 10/300GL columns (GE Healthcare) with 20 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: α-Syn was further purified with Ni-NTA affinity chromatography using a HisTrap HP 5 mL column (GE healthcare life sciences, USA) connected to an ÄKTA system (GE healthcare life sciences ...
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bioRxiv - Microbiology 2020Quote: ... All recombinant pilins were produced with an N-terminal histidine tag for immobilized nickel affinity purification using 1ml HisTrap columns (GE Healthcare) per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The liberated affinity-tag and the His-tagged Senp1 protease were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75 or 200 pg ...
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bioRxiv - Immunology 2019Quote: ... Strep(II)-tag proteins were purified using affinity column chromatography using a StrepTrap™ column prepacked with StrepTactin Sepharose by following the manufacturer’s protocol (GE Healthcare). The VLP recombinant proteins were further purified by size exclusion chromotogrophy (Superose 6 ...
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bioRxiv - Neuroscience 2021Quote: ... and purified from culture supernatants by an immobilised metal affinity using an automated protocol implemented on an ÄKTAxpress (GE Healthcare, UK) followed by a Superdex 200 10/300GL column ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) using a prepacked 5 ml His-Trap HP column (GE Healthcare). The purified recombinant proteins were buffer-exchanged in 1 × PBS ...
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bioRxiv - Microbiology 2020Quote: ... GST-FliJ(Δ13–24) and GST-FlgN were purified by GST affinity chromatography using a glutathione Sepharose 4B column (GE Healthcare) as described previously28 ...
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bioRxiv - Immunology 2020Quote: ... was further purified by cation exchange and heparin affinity chromatography prior polishing by gel filtration through a Superdex 200 16/40 column (GE Healthcare), which was operated in 300 mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... Clarified Sf9 supernatants were filtered and MuSK1A was purified by immobilized metal affinity chromatography (IMAC) (Ni Sepharose excel, GE Life Sciences) followed by size exclusion chromatography (Superdex 200 Increase 10/300 GL column ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed using a French press and recombinant proteins were purified by nickel ion affinity chromatography (HisTrap HP, 1ml - GE Healthcare). The His6-SUMO tag was removed using Ulp1 protease (Li and Hochstrasser ...
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bioRxiv - Biochemistry 2019Quote: ... All Rab proteins were purified from the supernatant by nickel affinity chromatography (5 ml HiTrap™ Chelating HP column, GE Healthcare). Non-specifically bound proteins were removed by a step wash with buffer B (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... Solubilized IBs were purified by immobilized metal affinity chromatography (IMAC) by loading onto a prepacked 1 ml His-Trap HP column (GE Healthcare) equilibrated with lysis buffer containing 8M urea at a flow rate of 1 ml/min using an AKTA-pure-25 FPLC (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: Human his-tagged TRMT6/61A dimer was purified from Escherichia coli BL21 cells using 1 mL HisTrap HP affinity chromatography columns (GE Healthcare). Eluted fractions containing the recombinant proteins were pooled and incubated overnight with PreScission protease at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The protein was purified by submitting the supernatant to immobilized metal affinity chromatography (IMAC) using prepacked columns with Ni-Sepharose (HisTrapTM, GE Healthcare) in an ÄKTA Star protein purification system (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were expressed transiently in HEK-293T cells and affinity purified from the culture supernatant by protein-A sepharose beads (GE Healthcare) or streptactin beads (IBA ...
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bioRxiv - Microbiology 2020Quote: ... Diafiltrated cell supernatant was purified by immobilized nickel affinity chromatography (5-ml fast flow crude column and ÄKTA fast protein liquid chromatography [FPLC] system; GE Healthcare) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... eluted fraction were set aside after the first step of Ni affinity chromatography and directly loaded on a Superdex 200 Increase (10/300 GL) column (GE Healthcare) and eluted in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... The N and RBD proteins contained a histidine tag and were purified through affinity chromatography step with the Histrap HP (GE HealthCare) column following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and AAV2-CMV-mKO) were produced using a helper-free triple transfection procedure and purified via affinity chromatography (GE Healthcare, USA).
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bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
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bioRxiv - Biophysics 2021Quote: ... The cells were then burst open at 19 psi using French press followed by collection of cell lysate and purification using Ni2+-NTA affinity column of AKTATM Pure (GE Healthcare). The proteins were purified using 20 mM imidazole containing binding buffer and 250 mM imidazole containing elution sodium phosphate buffer (pH 7.4) ...
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bioRxiv - Plant Biology 2021Quote: The banding affinity between Arabidopsis NDH-PSI and PSI with Fd was detected by SPR using a Biacore T100 biosensor (GE Healthcare). The experiment was performed as previously described with some modifications19 ...
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bioRxiv - Cell Biology 2020Quote: Secreted WNTs were enriched from cell culture supernatants using the affinity chromatography resin Blue Sepharose 6 Fast Flow (17-0948-01, GE Healthcare) and analysed by Western blotting according to previously published protocols (Glaeser et al. ...
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bioRxiv - Biophysics 2021Quote: ... U-13C-enriched proteins were purified through Ni-NTA affinity column and further by gel filtration chromatography on an ÄKTA Pure 25 FPLC (GE Healthcare) using Superdex 75 Increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: Fab and scFv constructs were eluted from affinity purification and then purified further via size exclusion chromatography using the Superdex 200 Increase 10/300 GL column (GE HealthCare) and 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... and the soluble fraction was column-purified by immobilized metal ion affinity chromatography using Nickel sepharose high performance beads (17526802, GE Healthcare). The eluate was fractionated by gel filtration on a Superdex-200 Increase 10/300GL column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: Purified TlpALBD was immobilized on a CM5 series S sensor chip and binding affinities tested using a BIAcoreTM S200 instrument (GE Healthcare) as described (Day et al. ...
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bioRxiv - Microbiology 2021Quote: ... the pure ParD2-ParE2 complex in SEC buffer was loaded onto a nickel–nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated in the same SEC buffer ...
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bioRxiv - Immunology 2021Quote: ... Purification of E-301 and E-301-LOF was performed directly from transfection harvests using a HiTrap Protein A affinity column (GE Healthcare) and eluted with 1M arginine pH 3.9 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was further purified by Ni-NTA immobilized metal-affinity chromatography (IMAC) using five milliliter His-Trap HP columns (GE Healthcare), followed by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2022Quote: ... Antibody supernatant was harvested 6 days following transfection and purified using protein G affinity chromatography following the manufacturers protocol (GE Healthcare). Specificity of the cloned mAb was determined by ELISA.
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease as described previously32 ...
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bioRxiv - Cancer Biology 2022Quote: ... The binding affinity was determined by global fitting to a Langmuir 1:1 binding model within the Biacore Evaluation software (GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was first purified by Immobilized Metal Affinity Chromatography (IMAC) by loading the clarified lysate onto a 5 mL Nickel column (GE Healthcare) pre-equilibrated in buffer A ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates from 8 L of yeast treated cells were then subjected to a 5 ml Nickel affinity purification using an AKTA purifier system (GE Healthcare) equilibrated in the urea buffer ...
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bioRxiv - Cell Biology 2022Quote: ... His8-tag containing recombinant proteins were purified using Ni2+-NTA agarose affinity column followed by size exclusion chromatography (Superdex 200 26/60 column from GE healthcare) in a final buffer containing 100 mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... The supernatant was harvested and the protein was purified by FLAG-affinity purification followed by MonoQ anion exchange chromatography (GE Healthcare). Finally ...
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bioRxiv - Immunology 2022Quote: ... The eluted product from kappa affinity column was pooled and further purified by size-exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare) equilibrated with a buffer containing 20 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease ...