Labshake search
Citations for Quantifoil Micro Tools :
601 - 650 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Solutions were adjusted to OD600≈10 and applied to carbon-coated holey copper grids (Quantifoil R2/1). Grids were glow-discharged for 30 s in oxygen/argon atmosphere of a plasma cleaner (Gatan Solarus 950 ...
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bioRxiv - Biochemistry 2022Quote: ... holey copper grids (QUANTIFOIL R 2/2). 3.5-μl samples were adsorbed for 1 min at +20 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 300 mesh copper grids (Quantifoil) were glow discharged using a Tergeo Plasma Cleaner (Pie Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biochemistry 2022Quote: ... R3/3 copper grids with a 2 nm carbon coating (Quantifoil) using a Vitrobot mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of sample was applied to a plasma treated gold coated R 1.2/1.3 300 mesh holey carbon grid (Quantifoil), with a blot force of 0 ...
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bioRxiv - Biochemistry 2022Quote: ... the crosslinked HSP90-CDC37-BRAFV600E-PP5 complex was concentrated to 1μM and 3μl of the sample was applied to carbon grids (Quantifoil R1.2/1.3, Cu, 300 mesh) which were glow discharged using a Tergeo Plasma Cleaner (Pie Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3.5 μL was applied to freshly glow-discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: Quantifoil R2/2 Cu300 or Quantifoil R1.2/1.3 Cu300 (Quantifoil Micro Tools) grids were glow-discharged using the GloQube plasma cleaner (Quorum ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The proteins were incubated at 37°C and 200 rpms for 10 minutes on a microplate shaker (Quantifoil Instruments, Jena, Germany). Next ...
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bioRxiv - Microbiology 2022Quote: ... 3 mm gold EM finder grids with a perforated carbon film (R 2/2, 200 mesh) were purchased from Quantifoil (AU G200F1 finder ...
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bioRxiv - Plant Biology 2022Quote: 3 μL of purified PSI complex at 1 mg Chl mL−1 were applied to glow-discharged (GloQube Quorum, 40 sec, 20mA) holey carbon grids (Quantifoil 300 Au R1.2/R1.3 ...
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bioRxiv - Molecular Biology 2022Quote: 3 μL of ~120 nM mitoribosome was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/2, copper, mesh 300) coated with continuous carbon (of ~3 nm thickness ...
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bioRxiv - Molecular Biology 2022Quote: 3.0 μl of purified complex was applied to glow-charged 300 mesh holey carbon grid (Quantifoil Au R1.2/1.3). Grids were blotted for 3.0-4.0 s at a blotting force of 4 and vitrified using a Vitrobot MarkIV (Thermo fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: The concentrated sample (3.5 μL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of protein sample was applied to holey carbon grids (Quantifoil, R 0.6/1 Cu 200 mesh) which were H2/O2 glow-discharged for 30 s ...
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bioRxiv - Microbiology 2022Quote: ... 3 μl of spike-Fab complex was applied to glow-discharged (20 mAmp, 30 sec, Quorum GloQube) Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH), blotted for 5 s using blot force 2 and plunge frozen into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μL of sample solution was applied onto a holey carbon grid (Quantifoil R2/1, Cu 300 mesh) covered with a thin amorphous carbon film ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μL of purified OTR:OT:Go/q:scFv16 complex in blotting buffer were applied to glow-discharged holey carbon gold grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 3.5 μL of purified protein at a concentration of 0.22 mg/mL was applied to Graphene Oxide Au 400 mesh QUANTIFOIL R1.2/1.3 holey carbon grids (Quantifoil), and then plunged into liquid nitrogen-cooled liquid ethane with a FEI Vitrobot Mark IV (FEI Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... and then loaded onto glow-discharged (60 s at 20 mA) holey carbon Quantifoil grids (R1.2/1.3, 200 mesh, Quantifoil Micro Tools) using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μL of cell suspension was applied on glow-discharged copper or gold EM grids (R2/2, Quantifoil) and automatically back-blotted (Weiss et al 2017 ...
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bioRxiv - Biochemistry 2022Quote: UltrAuFoil gold grids (0.6/1, Quantifoil Micro Tools GmbH)56 were prepared as described previously9 ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μL of sample was applied to 200-mesh copper R2/1 grids (Quantifoil), which were glow-discharged twice using a Pelco EasyGlow instrument ...
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bioRxiv - Biochemistry 2022Quote: ... holey carbon grids (Quantifoil R 1.2/1.3, Micro Tools GmbH, Germany). The grids were blotted for 4 seconds and vitrified by rapidly plunging into liquid ethane at −182 °C using Leica EM GP plunger (Leica Microsystems ...
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bioRxiv - Biochemistry 2022Quote: Vesicles for cryo-ET were prepared similarly using 1.2/1.3 μm holey gold grids (Quantifoil UltrAuFoil) and blotted for 10 s using a Vitrobot Mark IV (ThermoFisher) ...
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bioRxiv - Biochemistry 2022Quote: ... with a surface-active additive (0.01% (w/v) glycyrrhizic acid) was added to a glow discharged (PELCO easiGlow) holey gold grid (Quantifoil UltrAuFoil grids (R0.6/1 ...
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bioRxiv - Biochemistry 2022Quote: ... Au 300 mesh (Quantifoil). Cryo-EM grids were prepared by manually freezing using a manual plunger in cold room at 4°C and stored in liquid nitrogen for future data acquisition ...
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bioRxiv - Microbiology 2022Quote: ... EM grids (R2/2, Cu 200 mesh; Quantifoil Micro Tools GmbH) were glow-discharged for 45 sec at 25 mA by PELCO easiGlow discharger ...
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bioRxiv - Microbiology 2022Quote: Copper grids coated with holey carbon film (Quantifoil Cu 1.2/1.3, 300 mesh) were glow-discharged for 30 s at 0.45 Torr ...
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bioRxiv - Biochemistry 2022Quote: 4 µl aliquots of purified samples at A280 = 1 were applied onto glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3), then adsorbed for 10 s ...
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bioRxiv - Biochemistry 2022Quote: 3 µl of prepared complexes at a concentration of approximately 1 mg/ml was applied to a glow-discharged Quantifoil 300 mesh copper R1.2/1.3 grids (Quantifoil Micro Tools). The grids were then flash frozen in liquid ethane using a Thermo Fisher Scientific Vitrobot IV (0-0.5 s waiting time ...
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bioRxiv - Biochemistry 2022Quote: 2.8 µl of F-actin sample (3 – 16 µM) was pipetted onto a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). After incubating for 1 – 2 seconds ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μl of the mixture was deposited on glow discharged R2/2 Copper 300 mesh holey carbon-coated support grids (Quantifoil, Jena, Germany) along with 1 μl of HEPES pH 10 and 1 μl of hydrogen peroxide 0.9 % ...
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bioRxiv - Immunology 2022Quote: ... detergent at a final concentration of 0.005 mM was added to each sample and 3 µl of that solution was immediately loaded onto plasma-cleaned QuantiFoil R 2/1 (400-mesh; Quantifoil Micro Tools GmbH) and UltrAuFoil R 1.2/1.3 grids (300-mesh ...
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bioRxiv - Immunology 2022Quote: ... and UltrAuFoil R 1.2/1.3 grids (300-mesh; Quantifoil Micro Tools GmbH). The plasma cleaning step was performed in the Solarus 950 plasma system (Gatan ...
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bioRxiv - Immunology 2022Quote: ... 4.0 µl of the complex was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlowTM Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Immunology 2022Quote: ... and the mixture was immediately applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3) in an FEI Vitrobot IV (4 °C and 100% humidity) ...
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bioRxiv - Biophysics 2022Quote: ... 300 mesh R 1.2/1.3 gold grids (Quantifoil, Großlöbichau, Germany) and plunge frozen in liquid ethane using a FEI Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: UltraAUfoil R1.2/3 (Quantifoil Micro Tools GmbH) grids were glow-discharged on both sides for 30 s at 15 mA using a Pelco Easiglow ...
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bioRxiv - Biophysics 2022Quote: ... 10 µl sample was applied onto a glow discharged copper grid coated with holey carbon (R 2/2; Quantifoil, Jena, Germany), and subsequently dipped onto 500 µl deionized H2O for 1 second to clear the sucrose ...
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bioRxiv - Biophysics 2022Quote: ... except for using copper grid coated with holey carbon (R 1.2/1.3; Quantifoil, Jena, Germany), applying 4 µl virus sample and not adding fiducial beads ...
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bioRxiv - Biophysics 2022Quote: ... 3 µl of DeCLIC sample was applied to a glow-discharged quantifoil 1.2/1.3 Cu 300 mesh grid (Quantifoil Micro Tools), which was then blotted for 1.5 s and plunge-frozen into liquid ethane using a FEI Vitrobot Mark IV ...
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bioRxiv - Biophysics 2022Quote: ... UltrAuFoil 1.2/1.3 300 mesh grids (Quantifoil) were plasma treated and vitrified samples were prepared by adding a 2.5 μL droplet of sample solution to a grid ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of sample (at the native 1 nM concentration) on glow discharged holey gold grids (Quantifoil Cu 1.2/1.3 400 mesh). Excess sample was removed by blotting with filter paper for 4 sec prior to plunge-freezing in liquid ethane using a FEI Vitrobot Mark IV at 100% humidity and 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 3.0 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 1 s at 100 % humidity ...
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bioRxiv - Biochemistry 2022Quote: Three microliters of Ct80S-RAC pull-out sample at 200 nM concentration was applied on holey carbon grids (Quantifoil R2/1 grid ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL of sample was applied to glow-discharged 200-mesh copper 2 nm C R1.2/1.3 cryo-EM grids (Quantifoil Micro Tools), blotted 3 s at 75 % humidity ...
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bioRxiv - Bioengineering 2022Quote: ... Holey carbon Quantifoil grids (2 µm diameter with an interspace of 2 µm, Quantifoil Micro Tools GmbH, Germany) were prepared by glow discharge (easyGlow® System ...
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bioRxiv - Biochemistry 2022Quote: Three microliters of Ct80S-RAC pull-out sample at 200 nM concentration was applied on holey carbon grids (Quantifoil R2/1 grid, Quantifoil Micro Tools, GmbH) and plunged-frozen into liquid ethane using a Vitrobot (FEI) ...