Labshake search
Citations for Quantifoil Micro Tools :
551 - 600 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Three μl of the sarkosyl-insoluble fractions were applied to glow-discharged (Edwards S150B) holey carbon grids (Quantifoil AuR1.2/1.3, 300 mesh) that were plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: R2/2 Cu 200 mesh grids (Quantifoil) were glow-discharged for 40 s at 2.7 mA ...
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bioRxiv - Biochemistry 2023Quote: ... applied to glow-discharged holey carbon EM grids (Quantifoil, Jena, Germany), and blotted for 5-6 seconds ...
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bioRxiv - Molecular Biology 2023Quote: UltrAuFoil holey grids (300 mesh R1.2/1.3, Quantifoil) were plasma-cleaned for 7 s using oxygen and argon gases with a Solarus II Gatan Plasma System ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 ul aliquot of the mixture was adsorbed onto a glow-discharged Quantifoil R 1.2/1.3 300 mesh grid (Quantifoil) and vitrified in liquid ethane with a Leica EM GP2 cryoplunger (Leica ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantifoil R1.2/1.3 400-square mesh copper grids with a 2 nm continuous carbon layer (Quantifoil) were plasma-treated in a Pelco easiGlow glow discharging system (Ted Pella ...
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bioRxiv - Immunology 2023Quote: ... Holey carbon grids (Cu 200 mesh, R2/1, Quantifoil®) were plasma-cleaned for 10 s in a Gatan Solarus 950 (Gatan) ...
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bioRxiv - Genetics 2023Quote: ... UltrAuFoil R1.2/1.3 Holey Gold foil on Gold 300 mesh grids from Quantifoil were glow-discharged for 90 s at 15 mA using the GloQube Plus Glow Discharge System from Quorum ...
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bioRxiv - Bioengineering 2023Quote: ... These samples were applied onto UltrAuFoil R 1.2/1.3 grids (Au, 300-mesh; Quantifoil Micro Tools GmbH). Prior to sample application the grids were glow-discharged using Ar/O2 plasma for 10 s on a Solarus 950 plasma cleaner (Gatan) ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Biochemistry 2022Quote: ... a 3.0 µL drop was applied to glow discharged holey carbon (R 1.2/1.3; Quantifoil), then blotted for 3 s ...
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bioRxiv - Molecular Biology 2022Quote: ... were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Excess samples were blotted for 4 s with a blot force of 10 and were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... 3 μl aliquots of the mixture was applied to R2/2 200 mesh copper Quantiofoil grids (Quantifoil Micro Tools) that were freshly glow discharged ...
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bioRxiv - Immunology 2022Quote: ... were incubated for 3 min at room temperature and then loaded onto the glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3). The grids were washed using the buffer containing 20 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... cells were seeded on R2/2 gold UltrAufoil grids (200 mesh, Quantifoil Micro Tools GmbH ...
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bioRxiv - Molecular Biology 2022Quote: Purified SARS-CoV-2 spike samples in 10mM Hepes pH7.2 and 150mM NaCl (3 μL at 0.62-0.75mg/ml) were kept at 4C until their application onto QUANTIFOIL R 1.2/1.3 Cu:300-mesh grids (QUANTIFOIL). The grids were vitrified using a Leica GP automatic vitrification robot (Leica) ...
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bioRxiv - Biochemistry 2022Quote: R2/2 holey carbon supported copper grids (Quantifoil) coated with a 2nm thin carbon layer were treated as described (A ...
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bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Quantifoil R2/1 grid: Cu 300mesh, M2951C-1-300) were hydrophilized by a JEC-3000FC Auto Fine Coater (JEOL ...
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bioRxiv - Bioengineering 2022Quote: ... A total of 3.5 μL of TP–AG treated MT samples was applied to a glow discharged holey carbon grid (Quantifoil R2/1). The grids were blotted ...
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bioRxiv - Biophysics 2022Quote: ... and DOR were prepared on Quantifoil R1.2/1.3 gold mesh grids with either holey carbon or UltrAuFoil films (Quantifoil, Germany). The holey carbon film grids were pre-cleaned in chloroform for 2-3 h at room temperature and allowed to dry overnight prior to use while the ones with UltrAuFoil film were used without any cleaning ...
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bioRxiv - Biophysics 2022Quote: A volume of 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biophysics 2022Quote: ... R2/2 Cu 300 grids (Quantifoil) were glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device ...
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bioRxiv - Biophysics 2022Quote: ... an aliquot of 3 μL of sample was applied onto a glow-discharged holey carbon-coated copper grid (300 mesh, QUANTIFOIL® R 2/1) or holey gold grid (300 mesh ...
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bioRxiv - Biophysics 2022Quote: ... a 3 μL aliquot of the mixture was applied to glow-discharged R1.2/1.3 300 mesh holey carbon gold grids (Quantifoil). After a 15 s incubation ...
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bioRxiv - Cell Biology 2022Quote: 2 x 104 TauRD-Y* cells or 1 x 105 neurons were seeded on EM grids (R2/1, Au 200 mesh grid, Quantifoil Micro Tools) in a 35 mm dish or 24-well plate and cultured for 24 h or transduced with lentivirus and treated with aggregate-containing cell lysate as described earlier in section ‘Primary neuronal cultures’ ...
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bioRxiv - Cell Biology 2022Quote: ... and UV sterilized R ¼ Carbon 200-mesh gold electron microscopy (EM) grids (Quantifoil Micro Tools). After 15-18 hours of culture ...
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bioRxiv - Biophysics 2022Quote: ... the extra carbon coated gold Quantifoil grids (200 mesh, R 1.2/20, holey carbon film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Biophysics 2022Quote: ... Quantifoil grids (200 mesh Au R2/2 carbon or SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Immunology 2022Quote: ... 3 μl of sample was then dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 s at 20 mA ...
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bioRxiv - Biochemistry 2022Quote: ... proper dilutions were made to a final concentration of ∼.7 mg/mL and a 3.0 µL drop was applied to glow discharged holey carbon (R 1.2/1.3; Quantifoil), then blotted for 3 s ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were prepared by depositing 3 μl of the crystalline slurry onto a glow-discharged EM grid (Quantifoil, Cu 200 mesh, R2/2 holey carbon). Excess liquid was blotted away and the sample was vitrified using a Leica GP2 vitrification robot ...
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bioRxiv - Biochemistry 2022Quote: ... A 2.5 μL aliquot of the dialyzed complex was deposited onto gold UltrAuFoil R1.2/1.3 Au 300 mesh cryo-electron microscopy grids (Quantifoil) and plunge frozen into liquid-nitrogen cooled liquid ethane using a Mark IV Vitrobot (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 0.128 mM DDM) was applied to a plasma-cleaned UltraAuFoil R1.2/1.3 300-mesh grids (Quantifoil). Immediately after removing excess liquid by a filter paper (Whatman No ...
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bioRxiv - Biophysics 2022Quote: ... 4 µL of the freshly prepared mixture were applied to glow-discharged (Quorum GloQube) R1.2/1.4 Cu 200 (Quantifoil) grids ...
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bioRxiv - Biophysics 2022Quote: All samples were prepared on glow-discharged holey gold grids (Quantifoil ultrAufoil R1.2/1.3), blotted in a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and the mixture was immediately applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3) in an FEI Vitrobot IV (4 °C and 100% humidity) ...
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bioRxiv - Molecular Biology 2022Quote: RNA granule preparations were diluted with RNA Granule buffer to an optical density A260 = 2 U/mL aliquots of the dilution deposited on glow-discharged Quantifoil grids (Quantifoil 1.2/1.3, 300 mesh copper) pre-coated with a continuous layer of 3 nm carbon ...
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bioRxiv - Molecular Biology 2022Quote: Graphene oxide (GO) grids were prepared from Quantifoil R1.2/1.3 grids ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of diluted complexes were applied to a glow discharged 200-mesh holey carbon grid (Au 1.2/1.3 Quantifoil Micro Tools), blotted for 1.5-2.5 s at 90% humidity ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µL of the mixture were pipetted onto a copper EM grid with holey carbon film (R2/2, 200 mesh, Quantifoil Micro Tools GmbH, Q43486) that had been freshly glow-discharged for 30 s at -30 mAmp ...
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bioRxiv - Biophysics 2022Quote: Cryo-EM grids were prepared by applying 3 μL of protein to a glow-discharged QuantiAuFoil R1.2/1.3 300-mesh grid (Quantifoil, Germany) and blotted for 2.5 to 4 s under 100% humidity at 4 °C before plunging into liquid ethane using a Mark IV Vitrobot (FEI).
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bioRxiv - Biochemistry 2022Quote: 3.5 microliters of Grafix purified complex with ARE35 DNA (Fraction 11) was applied to glow discharged R1.2/1.3 holey carbon grids (Quantifoil) at 4ºC and plunge frozen in liquid ethane using a Vitrobot Mark IV (Thermofisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... 3 µl of TIR-SAVED complex was applied to Holey Carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh), glow discharged for 45 s at current of 45 mA in a EMITECH K100X glow discharger ...
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bioRxiv - Biochemistry 2022Quote: ... Before spotting onto R2/1 grids with an extra 2 nm carbon layer (Quantifoil, Inc), the complex was diluted to a final concentration of ∼100 nM and a 5-fold excess of Met-tRNAiMet ...
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bioRxiv - Biochemistry 2022Quote: ... Spike trimer - Fab complexes (4 μl) were applied to the pre-glow-discharged holey carbon grids (Quantifoil grid, Cu 300 mesh, R1.2/1.3). The grids were then blotted for 2 seconds with filter paper in 100% relative humidity and 8 °C and plunged into the liquid ethane to freeze samples using FEI Vitrobot system (FEI).
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bioRxiv - Biochemistry 2022Quote: ... Several serial dilutions were prepared and 3 µl of each sample was loaded on Quantifoil R2/2Cu/Rh 300 holey-carbon-supported grids (Quantifoil Micro Tools GmbH, Jena, Germany). Initial cryo-EM sample preparations showed clear protein aggregation ...
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bioRxiv - Biophysics 2022Quote: ... 3.6 μl were applied to glow-discharged gold holey carbon 2/1 300-mesh grids (Quantifoil). Grids were blotted for 4 s at 0 force and 1-s wait time before being vitrified in liquid propane using a Mark IV Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −15 and plunge-frozen in liquid ethane.
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bioRxiv - Biochemistry 2022Quote: ... 3 μl of sample were applied onto a freshly glow-discharged copper R2/1 300 mesh grid (Quantifoil), blotted for 8 s on both sides with blotting force −15 and plunge-frozen in liquid ethane.