Labshake search
Citations for Quantifoil Micro Tools :
901 - 950 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... After another 3 min at 37 °C the termination complexes were placed on ice for 15 min and subsequently applied to Quantifoil R2/2 holey carbon grids (Quantifoil Micro Tools) either coated with a thin continuous carbon film or with graphene oxide flakes (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2021Quote: Holey carbon EM grids (Quantifoil, Jena, DE) were glow-discharged under vacuum using an ELMO TM Glow Discharge System (Cordouan Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... 3.5 μL was applied to a glow-discharged Quantifoil copper grid with 2 nm continuous carbon support over holes (Quantifoil R 2/4 400 mesh), blotted ...
-
bioRxiv - Molecular Biology 2021Quote: QUANTIFOIL R 2/1 grids with 2nm carbon layer (Cu 200, Quantifoil Micro Tools) were glow discharged with 25 mA with negative polarity for 60 seconds in a PELCO easiGlow glow discharge unit ...
-
bioRxiv - Molecular Biology 2021Quote: The concentrated sample (3.5 μL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2021Quote: The purified and cross-linked recombinant NLRP3 protein was loaded onto glow-discharged EM-grids (Quantifoil, R2/1 Cu-covered with homemade ultrathin carbon support film) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and applied onto freshly glow-discharged 200 square mesh Ultrafoil R2/2 gold grids (Quantifoil). The samples were plunge-frozen with a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
-
A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: The purified PACAP27–VIP2R–Gs complex (3 μL at 3.7 mg per mL) was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Vitrification was performed using a Vitrobot Mark IV (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... Quantifoil 1.2/1.3 Cu 300 mesh grids (Quantifoil Micro Tools) were glow-discharged in methanol vapor prior to sample application ...
-
bioRxiv - Biochemistry 2021Quote: 2.5 μl of ACE2-RBD-62 complex at 3.5 mg/ml concentration was transferred to glow discharged UltrAuFoil R 1.2/1.3 300 mesh grids (Quantifoil), blotted for 2.5 seconds at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 µL of the concentrated RdRp-RNA complex were mixed with 0.5 µl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 7 seconds with blot force 5 using a Vitrobot MarkIV (Thermo Fischer Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... Cu R1.2/1.3 or R2/1 holey carbon grids (Quantifoil, Groslöbichau, Germany) were glow discharged at 25 mA for 45 seconds using an Emitech K950 apparatus (Quorum ...
-
bioRxiv - Biophysics 2021Quote: ... The SEC-purified complex was concentrated using a 50 kDa cut-off concentrator to 1.5 mg/ml and applied to glow-discharged (0.39 mbar, 15 mA, 30 s) holey carbon grids (QuantiFoil R1.2/1.3 Au 200 mesh). Surplus sample was blotted off for 2-4 seconds with zero blotting force in a controlled environment (4 °C ...
-
bioRxiv - Biophysics 2021Quote: ... 1.5 μl of THO*•Sub2 was applied to a glow-discharged UltrAuFoil R 1.2/1.3 grids (Quantifoil). Grids were blotted for 3 s with a blotting force of 3 and 100% humidity at 22 °C and plunged into liquid ethane using an FEI Vitrobot Mark IV (Thermo Fisher).
-
bioRxiv - Microbiology 2021Quote: The 2.5 µl sample of purified NP-RNA complex solution was applied onto glow-discharged 200-mesh R1.2/1.3 Cu grids (Quantifoil, Jena, Germany). The grids were blotted and rapidly frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were applied to glow-discharged holey carbon film grids (Quantifoil) and plunge frozen into liquid ethane using a Vitrobot (ThermoFisher) ...
-
bioRxiv - Microbiology 2021Quote: ... Freshly glow-discharged finder gold quantifoil grids (200 mesh R2/2 – Quantifoil MicroTools GmbH, Germany) were sterilized in 70% EtOH and placed in a glass-bottomed MatTek dish (MatTek Corporation ...
-
bioRxiv - Microbiology 2021Quote: ... holey carbon grids (Quantifoil R2/1 200 mesh copper) for 1 min ...
-
bioRxiv - Cell Biology 2021Quote: MEFs expressing emerald-Vimentin are cultured on glow-discharged holey carbon coated EM grids (Au R2/1, 200 mesh, Quantifoil) for 16 h at 37°C in a humidified CO2 incubator ...
-
bioRxiv - Biophysics 2021Quote: ... of purified CtenDNAV-II virons (10 mg ml-1) was deposited onto freshly glow-discharged holey carbon-coated copper grids (Quantifoil R 2/2, 300 mesh, copper) followed by 2 s of blotting in 100% relative humidity for plunge-freezing (Vitrobot Mark IV ...
-
bioRxiv - Biochemistry 2021Quote: ... or the copper grids (Quantifoil R1.2/1.3 Cu, 300 mesh) with a thin carbon-supported film glow-discharged for 70 s for the WT monomers ...
-
bioRxiv - Biophysics 2021Quote: ... 3 μl of samples were applied onto a glow-discharged gold grids covered with holey carbon film (Quantifoil, 300 mesh 1.2/1.3) and blotted using a Vitrobot Mark IV (FEI Company ...
-
bioRxiv - Biochemistry 2021Quote: UltrAuFoil 0.6/1 300 mesh grids (Quantifoil) were glow discharged in residual air at 25 mA for 3 min using a Pelco EasiGLOWTM ...
-
bioRxiv - Biochemistry 2021Quote: 5 μl of each ribosome sample at 2-4 units of UV 260nm absorbance was applied to a plasma cleaned 300 mesh UltraAufoil 1.2/1.3 grid (Quantifoil, Germany). The grids were blotted for 2-3 sec at 8 °C in 100% humidity (blot force 1 ...
-
bioRxiv - Biochemistry 2021Quote: ... UltrAuFoil R1.2/1.3 300 mesh gold grids (Quantifoil) were plasma cleaned (Pie Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... and 2.0 μl sample was applied to a glow-discharged holey carbon grid (R1.2/1.3, 200 mesh, Quantifoil). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... 2.0 μL of the protein sample was applied to 300 mesh Cu Quantifoil 1.2/1.3 grids (Quantifoil Microtools) that were glow discharged for 20 seconds at 25 mA ...
-
bioRxiv - Biophysics 2021Quote: To provide extra support for cell growth 5-6 nm of carbon was evaporated onto Quantifoil Au 200 R2/1 or R2/2 grids (Quantifoil Micro Tools GmbH). Grids were then glow discharged and incubated in supplemented RPMI-1640 overnight in the cell incubator ...
-
bioRxiv - Biophysics 2021Quote: ... aliquots (3 μL) of the concentrated protein were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3) and blotted for 5 s ...
-
bioRxiv - Biophysics 2021Quote: All samples were prepared on glow-discharged holey gold grids (Quantifoil ultrAufoil R1.2/1.3), blotted in an FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2021Quote: ... 4 µl of this mixture was applied on a glow-discharged Quantifoil R2/2 grid (Quantifoil Micro Tools GmbH) and flash-frozen in liquid ethane using a Vitrobot Mark II device (FEI) ...
-
bioRxiv - Microbiology 2021Quote: Elutions from pull-downs were kept on ice until being applied within two hours to glow discharged cryo-grids (Quantifoil 2/2 Cu300 coated with 2 nm continuous carbon) ...
-
bioRxiv - Microbiology 2021Quote: ... 5 µl of the cell suspension was placed on freshly glow-discharged (for ∼25 s) holey carbon grids (Quantifoil Cu R2/1, 200 mesh). The grids were front blotted with Whatman filter paper and rapidly frozen in liquid ethane ...
-
bioRxiv - Microbiology 2021Quote: ... 3μL of protein was deposited on a holey copper grids (QUANTIFOIL R 1.2/1.3, 200 mesh, EMS) which had been glow-discharged for 30s at 15 mA (Tedpella Inc) ...
-
bioRxiv - Biophysics 2021Quote: Holey carbon grids (Quantifoil Au 300 mesh, R 0.6/1) coated with home-made ultrathin continuous carbon were glow-discharged by plasma cleaner (Harrick ...
-
bioRxiv - Biophysics 2021Quote: ... UltrAuFoil 1.2/1.3® gold (Quantifoil) electron microscopy grids were glow discharged in air for 1 min at 0.3 mpa using a PELCO easiGlow™(Ted Pella ...
-
bioRxiv - Biophysics 2021Quote: ... 3.5 µl sample applied to a glow-discharged Quantifoil R1.2/1.3 400-mesh gold holey carbon grid (Quantifoil, Micro Tools GmbH, Germany), blotted under 100% humidity at 4 °C and plunged into liquid ethane using a Mark IV Vitrobot (FEI) ...
-
bioRxiv - Biophysics 2021Quote: ... Cryo-EM grids (Quantifoil AU 1.2/1.3 300 mesh) were glow-discharged and coated with graphene oxide thin layer flakes following the protocol from reference43 (figshare ...
-
bioRxiv - Biophysics 2021Quote: ... copper Quantifoil (Quantifoil Micro Tools GmbH) grids with 2 nm thick amorphous carbon on top ...
-
bioRxiv - Biophysics 2021Quote: Three microliters of purified PSI supercomplex was applied onto glow-discharged holey carbon grids (Cu Quantifoil R1.2/1.3) at a protein concentration of approximately 2.5 mg/ml ...
-
bioRxiv - Cell Biology 2021Quote: 3 mm gold EM grids with a holey carbon film (R 2/2, 200 mesh; Quantifoil Cat no ...
-
bioRxiv - Cell Biology 2021Quote: ... diluted to ~1,000 cells per μL) was blotted onto holey carbon-coated 200-mesh copper EM grids (Quantifoil Micro Tools) and plunge-frozen using a liquid ethane/propane mixture ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 mM TCEP and adsorbed to glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3) for 10 s ...
-
bioRxiv - Biochemistry 2021Quote: ... were applied to glow discharged (2.2×10-1 mbar for 20 sec) Quantifoil holey carbon grids (R2/1, 200 mesh, Quantifoil). The grids were plunge vitrified into a liquid ethane/propane mix using a Vitrobot Mark IV at 4 °C and 95% humidity ...
-
bioRxiv - Biochemistry 2021Quote: Three microliters of the h20S-PA26E102A-Opt5 solution were applied to R2/2 400-mesh grids (Quantifoil) that had been plasma treated for 25 s using a glow discharger (Electron Microscopy Sciences ...
-
bioRxiv - Biochemistry 2021Quote: ... 2.5-μL aliquots of purified Neo1 at a concentration of about 1 mg/mL were placed on glow-discharged holey carbon grids (Quantifoil Au R2/2, 300 mesh) and were flash-frozen in liquid ethane using a FEI Vitrobot Mark IV ...
-
bioRxiv - Biochemistry 2021Quote: ... A volume of 2.5 μL of each sample was applied on glow-discharged UltrAuFoil Au300 Rl.2/1.3 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
-
bioRxiv - Biochemistry 2021Quote: ... Quantifoil Cu 300 mesh (R 2/2 geometry; Quantifoil Micro Tools GMBH) covered with a thin layer of 3 nm carbon were used for the for the GTPBP5KO sample ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples were diluted twofold with Buffer F and 3.5 µl of sample was immediately applied onto a glow-discharged 300-mesh gold Quantifoil R1.2/1.3 grid (Quantifoil Micro Tools). Grids were blotted for 2-4 s and plunge-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot MarkIV (ThermoFisher ...
-
bioRxiv - Biochemistry 2021Quote: ... An aliquot of 3.5 μl of ∼13 μM E196K fibril solution was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh), blotted for 3.5 s and plunge-frozen in liquid ethane using an FEI Vitrobot Mark IV ...