Labshake search
Citations for Quantifoil Micro Tools :
701 - 750 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The complex (3 μL) was quickly applied on a glow-discharged UltraAuFoil R1.2/1.3 300 mesh holey Au grids (Quantifoil Micro Tools GmbH), blotted with Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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bioRxiv - Molecular Biology 2022Quote: Affinity-purified elongating ribosomes were vitrified on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil) coated with graphene oxide (GO) ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was then resuspended in warm EM buffer and 3 μL of this sample was applied to a carbon on gold grid (Quantifoil R2/2 Au 200 mesh), recently glow-discharged for 1 min at 30 mA ...
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bioRxiv - Molecular Biology 2022Quote: ... All Quantifoil/UltrAuFoil grids were purchased from Quantifoil Micro Tools GmbH.
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bioRxiv - Molecular Biology 2022Quote: ... either carbon on Cu/Rh (Quantifoil R2/2 200 mesh) or gold on gold UltrAuFoil R1.2/1.3 300 mesh ...
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bioRxiv - Biochemistry 2022Quote: Samples (3 μl) were applied onto a glow-discharged UltrAuFoil grid (Quantifoil GmbH) and were flash-frozen in liquid ethane using the Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: The holey carbon grids (Quantifoil Au R2/1, 300 gold mesh) were glow discharged in the Ar/O2 mixture for 30 s using a Gatan 950 Solarus plasma cleaning system with a power of 15 W ...
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bioRxiv - Biochemistry 2022Quote: Cryo-EM specimens were prepared on UltrAuFoil R1.2/1.3 holey gold grids (Quantifoil) that were glow-discharged for 20 s at 25 mA on both sides (PELCO easy glow) ...
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bioRxiv - Biophysics 2022Quote: ... holey carbon grid coated with 2 nm ultrathin carbon film (Quantifoil 300-mesh, R 2/2). After waiting for 60 s ...
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bioRxiv - Cell Biology 2022Quote: MEF were seeded on R2/2 holey carbon on gold grids (Quantifoil or Protochips) coated with fibronectin in a glass bottom dish (Mattek or Ibidi ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were applied to Quantifoil R1.2/1.3 Copper 300 mesh grids (Quantifoil Micro Tools), and were plunge-frozen with a EM GP2 (Leica ...
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bioRxiv - Biochemistry 2022Quote: ... Peak fractions were collected and 3 μl of TSEN/pre-tRNA complex were applied onto freshly glow-discharged copper grids (Quantifoil, Cu R1.2/1.3) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... the pelleted cells resuspended in 100 μl NaH2PO4-NaOH pH 7.5 and a 3 μl drop adsorbed for 20 s to a carbon-coated copper grid (300 mesh, Quantifoil) that had been glow-discharged for 30 s at a current of 25 mA ...
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bioRxiv - Biophysics 2022Quote: ... 4 μL of the TnsBCTD-TnsC-TniQ sample was loaded on UltrAuFoil R1.2/1.3 gold grids (Quantifoil) and vitrified using the Mark IV Vitrobot (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: Cryo samples were prepared on UltrAuFoil R1.2/1.3 300 mesh grids (Quantifoil), which were rendered hydrophilic through plasma cleaning for 1 min (Tedpella “Easy glow” discharge system ...
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bioRxiv - Biochemistry 2022Quote: The Cas9-RNA-DNA ternary complex at approximately 2.0 absorption units at 260 nm (A260) and in 4μl volume was applied to glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil). The sample was let to absorb for 10 seconds on girds followed by 2 seconds blotting at 19°C and 100% humidity ...
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bioRxiv - Biochemistry 2022Quote: ... three microliters of concentrated phage particles were added to a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany) and plunge-frozen with a Vitrobot Mk IV into a 50:50 mixture of liquid ethane:propane52
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... and 3 μl of the protein solution was applied to glow-discharged (45 s) Quantifoil R2/1 300-mesh gold grids (Quantifoil Micro Tools GmbH, Germany). The grids were blotted with standard Vitrobot filter paper for 2.5 s at 4 °C under 100% humidity and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... holey support film type R2/1 on 200 mesh copper grid (Quantifoil, Germany) that was previously glow discharged under the following conditions ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μl of complex mixture was applied on glow-discharged holey carbon grids (Quantifoil R1.2/1.3, Au 200 mesh) and excess of liquid was removed by blotting for 2-4 s in controlled environment (4°C ...
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bioRxiv - Biochemistry 2022Quote: ... gRAMPcrRNA-TPR-CHAT-target RNAself and gRAMPcrRNA-TPR-CHAT-target RNAnon-self complexes were applied onto glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil), respectively ...
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bioRxiv - Biochemistry 2022Quote: TEM gold grids with Holey Carbon support film (Quantifoil Micro Tools GmbH, Au 200 mesh R3.5/1) were glow-discharged in a plasma cleaner (Diener electronic GmbH ...
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bioRxiv - Bioengineering 2022Quote: ... Harrick Plasma PDC-002-EC instrument) holey carbon-coated grid (copper 200 mesh R1.2/1.3, Quantifoil). After a 1 min incubation ...
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bioRxiv - Biophysics 2022Quote: ... Supporting grids for electron microscopy with periodic holes in an amorphous carbon film (Quantifoil R1.2 / 1.3, Quantifoil) were processed in a glow discharge using a PELCO easiGlow device under standard conditions ...
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bioRxiv - Biophysics 2022Quote: ... Supporting grids for electron microscopy with periodic holes in an amorphous carbon film (Quantifoil R1.2 / 1.3, Quantifoil) were processed in a glow discharge using a PELCO easiGlow device under standard conditions ...
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bioRxiv - Biochemistry 2022Quote: ... 20 mg/ml for the D5R-Rotigotine-Gs complex and 15 mg/ml for the D1R-Rotigotine-Gs complex were applied onto a glow-discharged holey carbon grid (Quantifoil R1.2/1.3). Grids were plunge-frozen in liquid ethane using Vitrobot Mark IV (Thermo Fischer Scientific) ...
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bioRxiv - Biophysics 2022Quote: ... 300 mesh R1.2/1.3 gold grids (Quantifoil). Sample were plunge frozen in liquid nitrogen cooled liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: R2/2 grids (Quantifoil) were covered with a thin layer of continuous carbon (estimated to be 50 Å thick ...
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bioRxiv - Biochemistry 2022Quote: UltrAuFoil on gold 200 mesh R2/2 grids (Quantifoil) were subjected to micropatterning using the Primo module from Alveole mounted on a Leica DMi8 microscope following the manufacturer procedure ...
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bioRxiv - Biochemistry 2022Quote: ... Three μL of sample at 5 μM was adsorbed onto glow-discharged copper Quantifoil R2/1 holey carbon grids (Quantifoil). Grids were glow discharged 45 seconds at 25 mA using an EMITECH K950 apparatus ...
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bioRxiv - Biochemistry 2022Quote: KRASGTPase/BRAFWT/MEK1SASA/14-3-3 complex in SEC buffer was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 400 mesh) and vitrified using a Leica EM GP ...
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bioRxiv - Biochemistry 2022Quote: Open-hole R1.2/1.3 grids (Quantifoil) with 200 mesh were used for plunge freezing ...
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bioRxiv - Biochemistry 2022Quote: UltrAuFoil® gold grids (0.6/1, Quantifoil) (Russo and Passmore ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL of the purified PTH-PTH1R-Gs and PTHrP-PTH1R-Gs complexes at a concentration of ∼8.4 mg/mL and ∼5.1 mg/mL were respectively applied to the glow-discharged Au 300 mesh holey carbon grids (Quantifoil R1.2/1.3). The grids were blotted and then plunge-frozen in liquid ethane using a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... then 2.4 μL of sample mixture was transferred to a glow-discharged grid (Quantifoil R1.2/1.3, 300 Mesh, molybdenum) (Figure 2D) ...
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bioRxiv - Biochemistry 2022Quote: Standard holey carbon grids (Quantifoil, Cu 200 mesh, R2/2) were glow discharged for 30 s at 15 mA on the negative setting ...
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bioRxiv - Biochemistry 2022Quote: Freshly prepared ribosome–Sec61–KZR-8445 complex at 300–500 nM concentration was applied on glow discharged holey-carbon grids (Quantifoil, R1.2/1.3 with a 2-nm carbon film) and flash frozen with an automated plunge freezer (Leica microsystems) ...
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bioRxiv - Biochemistry 2022Quote: ... 2–2.5 µl of protein solution was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 Au 200 mesh). The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C ...
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bioRxiv - Biochemistry 2022Quote: ... The endogenous samples were applied onto grids containing an additional thin layer of continuous carbon support (Quantifoil R1.2/1.3 Cu 200 mesh + 2 nm C) and incubated for 30 sec before blotting and freezing ...
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bioRxiv - Biochemistry 2022Quote: ... and an aliquot of 2 μl of this sample was applied onto a plasma cleaned holey carbon grid (Quantifoil, R1.2/1.3, 200 mesh). The grid was blotted with Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μL of purified CB1-Gi complex at 15 mg/ml was applied on glow- discharged holey carbon gold grids (Quantifoil R1.2/1.3, 200 mesh). The grids were blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: ... were prepared by applying 3 μL of freshly prepared STC (1:2 dilution) to UltrAuFoil 300 mesh R1.2/1.3 holey grids (Quantifoil), glow-discharged for 60 s at 10 mA (Leica EM ACE200) ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then applied to a gold Quantifoil R 1.2/1.3 holey carbon grid (Quantifoil) that was glow discharged for 35 sec using PELCO easiGlow glow discharge cleaner ...
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bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Ultrafoil 25 nM Au R 0.6/1 300 mesh; Quantifoil) were incubated with a thiol reactive ...
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bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Ultrafoil 25 nM Au R 2/2 200 mesh; Quantifoil) were glow-discharged for 10 s using the Solarus plasma cleaner (Gatan) ...
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bioRxiv - Bioengineering 2022Quote: ... applied to a holey carbon grid (Ultrafoil 25 nM Au R 1.2/1,3 300 mesh; Quantifoil) pretreated with SAM solution ...
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bioRxiv - Biophysics 2022Quote: ... (Quantifoil Micro Tools GmbH) and cryo plunged in liquid ethane after blotting using a Vitrobot device (MK4).
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bioRxiv - Biophysics 2022Quote: ... for 25 s) grid (For GDN 150mM: UltrAuFoil 300 mesh R 1.2/1.3, other samples: gold holey carbon Quantifoil 300 mesh R 1.2/1.3). After a ∼5 s manual wait time ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...