Labshake search
Citations for Quantifoil Micro Tools :
751 - 800 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: Purified 8C-8A(IL125) was applied to 300 mesh UltrAuFoil holey gold 1.2/1.3 grids (Quantifoil Microtools) that were glow discharged for 10 seconds at 25 mA ...
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bioRxiv - Cell Biology 2022Quote: Cells were trypsinized and seeded onto 200 mesh gold holey carbon grids (R2/2-2 C, Quantifoil Micro Tools) placed in 3D printed grid holders (51 ...
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bioRxiv - Cell Biology 2022Quote: ... The IL-11Δ10/IL-11RαD1-D3/gp130D1-D3 complex (0.5 mg/mL) was blotted onto UltrAuFoil grids (R2/2, Quantifoil Micro Tools GmbH) that had been subjected to glow discharge (15 mA for 30 s) ...
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bioRxiv - Cell Biology 2022Quote: Tfam+/− MEFs (TFAM Het MEF) and SV40 mouse embryonic fibroblasts (SV40 MEF) were cultured on R ¼ Carbon 200-mesh gold electron microscopy grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture using a Vitrobot Mark 4 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were seeded on EM grids (Quantifoil SiO2 R2/2, 200 mesh, gold) in a 3D printed specimen holder [43] and grown overnight.
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bioRxiv - Immunology 2022Quote: ... UltrAuFoil R1.2/1.3 (Au, 300-mesh; Quantifoil Micro Tools GmbH) grids were treated with Ar/O2 plasma using a Solarus plasma cleaner (Gatan ...
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bioRxiv - Immunology 2022Quote: A 3.5 μL sample of concentrated pNO-decorated CLPs was applied to negatively glow discharged (25 mA, 40 s) R3.5/1 quantifoil copper grids (Quantifoil Micro Tools). The excess of solution was blotted using a Vitrobot Mark IV (FEI ...
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bioRxiv - Immunology 2022Quote: ... 2.2 µl of the purified mixture at concentration of 0.2 mg/mL was applied to glow discharged (40 s at 25mA) UltraAu grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
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bioRxiv - Immunology 2022Quote: ... 200 mesh (Quantifoil Micro Tools GmbH, Jena, Germany). Cells were then cultured in RPMI (24 h ...
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bioRxiv - Plant Biology 2022Quote: ... Holy carbon grids (Quantifoil Au 1.2/1.3, 300 mesh) were glow-discharged for 30 s at medium level in HarrickPlasma after 2 min evacuation ...
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bioRxiv - Molecular Biology 2022Quote: ... for 15 min before 4 μL of sample was applied to a graphene oxide coated 1.2/1.3 UltrAuFoil grids (Quantifoil) at 4°C under 100% humidity86 ...
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bioRxiv - Microbiology 2022Quote: Cryo-ET samples were prepared using copper grids with holey carbon support film (200 mesh, R2/1, Quantifoil). The grids were glow-discharged for ∼30 s before depositing 5 µl of cell solution onto them ...
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bioRxiv - Molecular Biology 2022Quote: ... 2.8 µl of sample were applied to holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 200 mesh), which were previously glow-discharged at 5 mA for 20 s ...
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bioRxiv - Molecular Biology 2022Quote: ... 2.8 µl of sample were applied to holey-carbon cryo-EM grids (Quantifoil Au R1.2/1.3, 300 mesh), which were previously twice glow-discharged at 15 mA for 45 s ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of the cells were applied to a glow-discharged 200 mesh holey carbon grid copper grid (Quantifoil R1.2/3) and vitrified in a liquid ethane on a Vitrobot Mark IV (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 200 mesh Cu grid (Quantifoil Micro Tools GmbH, Jena, Germany) and incubated 30 s before blotting at 20 °C with approximately 95% relative humidity ...
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bioRxiv - Microbiology 2022Quote: ... the purified full-length BA.2 spike protein at 4.6 mg/ml in DDM was applied to a 1.2/1.3 Quantifoil gold grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Microbiology 2022Quote: 3µl of enriched parasites were applied onto a freshly plasma-cleaned UltrAufoil R1.2/1.3 300 mesh EM grid (Quantifoil) in a humidity controlled facility ...
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bioRxiv - Microbiology 2022Quote: Three-microliter aliquots of all AdSDV capsid populations (~1 mg/mL) were applied to glow-discharged Quantifoil holey carbon grids with a thin layer of carbon (Quantifoil Micro Tools GmbH) and vitrified using a Vitrobot Mark IV (FEI ...
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bioRxiv - Microbiology 2022Quote: ... The mixtures were deposited on freshly grow-discharged cryo-EM grids (Quantifoil R2/1 ...
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bioRxiv - Microbiology 2022Quote: 3 μl of 529-nt nanorods (1.01 x 1015 particles/ml) were applied to glow-discharged R1.2/1.3 Cu 300 mesh grids (Quantifoil) and frozen on a Mark IV Vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of 4 μL samples were applied to glow-discharged holey carbon girds (Cu, R1.2/1.3, 300 mesh, Quantifoil). The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: A 5 µl cell suspension containing 10 nm colloidal gold particles were deposited on the copper EM grids with holey carbon supported film (Quantifoil, 200mesh, R1/2). The EM grid were blotted with filter paper (Waterman ...
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Mycobacteria form viable cell wall-deficient cells that are undetectable by conventional diagnosticsbioRxiv - Microbiology 2022Quote: ... of which 3-4 µL was applied on glow-discharged 200 mesh copper grids with an extra thick R2/2 carbon film (Quantifoil Micro Tools). Vitrification was performed using an automated Leica EM GP plunge-freezer ...
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bioRxiv - Microbiology 2022Quote: ... A 4□μl volume of SsoCsm complex was applied to holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh) covered by a graphene oxide layer (27) ...
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bioRxiv - Microbiology 2022Quote: ... Quantifoil (R2/2, Quantifoil Micro Tools) Au-EM finder grids ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically blotted from the backside for 4-6 s in a Mark IV Vitrobot by using a Teflon sheet on the front pad ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Microbiology 2022Quote: ... Purified BfpD was diluted to 4.5 mg/ml and 3 µl was applied to a glow-discharged 300 mesh UltraAufoil -1.2/1.3 holey-gold (Quantifoil, Germany). Grids were blotted for 2 s with ash-free Whatman® Grade 540 filter paper in a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The 300 mesh UltraAufoil -1.2/1.3 holey-gold (Quantifoil, Germany) grids were cleaned with a customized protocol (34 ...
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bioRxiv - Microbiology 2022Quote: ... loaded onto glow discharged carbon grids (R2/2, Quantifoil) and plunge-frozen using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Samples (3 µL) were applied to glow-discharged Quantifoil R1.2/1.3 Cu/Rh 200 mesh grids (Quantifoil) (M4R-Gi1-Ipx and M4R-Gi1-Ipx-LY298 ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2022Quote: The samples were frozen on copper or gold grids with holey carbon film coated with a 2 nm continuous carbon film (Quantifoil R1.2/1.3 ultrathin carbon, 300 mesh) freshly glow discharged for 20 s at 15 mA (PELCO easiGlow) ...
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bioRxiv - Microbiology 2022Quote: Phage particles were negatively stained for 20 s with 2 % uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Neuroscience 2022Quote: Samples were applied to glow-discharged holey carbon gold grids (Quantifoil R1.2/1.3, 300 mesh), and plunge frozen in liquid ethane using an FEI Vitrobot Mark IV ...
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bioRxiv - Microbiology 2022Quote: ... 2.7 μl of freshly purified sample was applied onto glow-discharged (30 s, 5 mA) grids (Quantifoil R 0.6/1 300 mesh Cu or Ultrafoil 0.6/1 300 mesh Au ...
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bioRxiv - Biochemistry 2022Quote: ... Raw movies from Quantifoil and graphene grid were collected in K3 super-resolution mode at a magnification of 81,000× (slit width 20 eV ...
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bioRxiv - Biochemistry 2022Quote: A total of 3880 (graphene grid) and 3075 (Au Quantifoil grid) raw movie stacks were automatically collected on a 300 kV Titan Krios using a K3 direct electron detector (with a BioQuantum energy filter ...
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bioRxiv - Biochemistry 2022Quote: ... The reported resolutions of the final maps (2.4 Å for the graphene grid and 2.9 Å for the Au Quantifoil grid) were estimated using the map versus model FSC curves (FSC0.5map vs ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The proteins were incubated at 37°C and 200 rpms for 10 minutes on a microplate shaker (BioShake iQ; Quantifoil Instruments ...
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bioRxiv - Microbiology 2022Quote: ... 5 µL of the mixture was placed onto freshly glow discharged holey carbon grids (Quantifoil™ R 2/1 on 200 copper mesh) for 1 minute ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μl sample were adsorbed for 45 s on glow-discharged 400 mesh R1.2/R1.3 holey carbon grids (Quantifoil) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... gold finder grids (Au NH2 R2/2, Quantifoil) containing infected amoebae were vitrified by back-blotting (Teflon ...
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bioRxiv - Microbiology 2022Quote: ... the amoebae (5×105 per well) were seeded onto EM gold finder grids (Au NH2 R2/2, Quantifoil) and incubated for 1 h ...
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bioRxiv - Neuroscience 2022Quote: Amplified fibril strains generated as described above were subjected to a brief water-bath sonication to disrupt larger aggregates for ~1 min in a water bath sonicator (Q-Sonica, Inc) and applied to plasma-cleaned (Pie Scientific) 1.2/1.3 UltrAufoil (Quantifoil) grids with a Leica GP2 ...
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bioRxiv - Molecular Biology 2022Quote: ... aliquots of 3 μl were applied to glow discharged grids (R2/1 or R1.2/1.3, Au 300, Quantifoil), blotted for 2 s and immediately plunge-frozen in liquid ethane using an FEI Vitrobot IV at 4°C and 100 % humidity ...
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bioRxiv - Molecular Biology 2022Quote: Quantifoil 200 mesh holey carbon R2/2 gold grids (Quantifoil Micro Tools) were glow discharged (PELCO easiGlow ...
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bioRxiv - Molecular Biology 2022Quote: R3.5/1 Holey carbon copper grids (QUANTIFOIL, Germany) were glow-discharged by UV light ...