Labshake search
Citations for Quantifoil Micro Tools :
951 - 1000 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... a fresh 400 mesh Quantifoil R1.2/1.3 Cu holey carbon grid (Quantifoil Micro Tools GmbH) was glow-discharged for 30 s using a Harrick plasma cleaner with technical air at 0.3 mbar and 7 W ...
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bioRxiv - Biochemistry 2021Quote: 3.5 µl protein solution containing 0.05 µM CUL4-NEDD8/ROC1/DDB1/DCAF1-CtD/Vprmus/SAMHD1 complex and 0.25 µM UBCH5C-ubiquitin conjugate (S4 A, B Fig) were applied to a 300 mesh Quantifoil R2/4 Cu/Rh holey carbon grid (Quantifoil Micro Tools GmbH) coated with an additional thin carbon film as sample support and stained with 2% uranyl acetate for initial characterisation ...
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bioRxiv - Biochemistry 2021Quote: ... C-flat grids (Protochips, USA; 1.2/1.3 300 mesh) and UltrAuFoil (Quantifoil, Germany; 1.2/1.3 300 mesh) were negatively glow discharged using PELCO Easiglow (Ted Pella ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μl of the freshly purified sample from the peak fraction in DDM at ~2.0 mg/ml for the B.1.1.7 protein or ~1.5 mg/ml for the B.1.351 protein was applied to a 1.2/1.3 Quantifoil grid (Quantifoil Micro Tools GmbH), which had been glow discharged with a PELCO easiGlow™ Glow Discharge Cleaning system (Ted Pella ...
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bioRxiv - Cell Biology 2021Quote: For each glow-discharged copper grid (Quantifoil Cu 200 mesh, holy carbon film R2/1) 4 μl of the supernatant were applied ...
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bioRxiv - Microbiology 2021Quote: A 2.5-ml suspension of the purified medusavirus particles was applied to a quantifoil grid (R1.2/1.3 Mo) (Quantifoil Micro Tools GmbH, Germany), which was glow-discharged beforehand for 30 s ...
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bioRxiv - Microbiology 2021Quote: ... 4-7 µl of cells were deposited on R2/1 Cu 200 grids (Quantifoil) that had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Immunology 2021Quote: ... R1.2/1.3 Cu 300 grids (Quantifoil) were glow discharged and coated with graphene oxide (0.2 mg/ml in ddH2O) ...
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bioRxiv - Microbiology 2021Quote: ... with the exception that Quantifoil Au R0.6/1 300 mesh grids (Quantifoil) were used ...
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bioRxiv - Microbiology 2021Quote: ... 3 µl of sample were applied to a freshly glow-discharged Quantifoil Au R2/2 200 mesh grid (Quantifoil), blotted for 5.5-7.5 s (0.5 s drain time ...
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bioRxiv - Microbiology 2021Quote: ... 3 µl of sample were applied to a freshly glow-discharged Quantifoil Au R2/2 200 mesh grid (Quantifoil), blotted for 3.5-7.5 s (0.5 s drain time ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of aldolase sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 6 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of apoferritin sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 7 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Microbiology 2021Quote: ... 3 μl of each sample was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using a PELCO easyGLow™ Glow Discharge Cleaning System (Tedpella) ...
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bioRxiv - Microbiology 2021Quote: ... 3 μL were applied onto a Cu grid with a holey carbon film (Quantifoil R 2/2; Quantifoil, Jena, Germany), blotted ...
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bioRxiv - Microbiology 2021Quote: ... 3 μL were applied onto a Cu grid with a holey carbon film (Quantifoil R 2/2; Quantifoil, Jena, Germany), blotted ...
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bioRxiv - Microbiology 2021Quote: We use Quantifoil carbon 200 mesh EM grids (Quantifoil, Jena, Germany). About 25 nm of carbon is sputtered onto the non-grid bar side of the grid using a Leica EM ACE600 High Vacuum Sputter Coater (Leica Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil). The grids were manually back blotted for 2 to 3s with filter paper ...
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bioRxiv - Microbiology 2021Quote: Holey carbon-coated gold Quantifoil® Finder grids (Quantifoil Micro Tools, Großlöbichau, Germany) R2/2 (reference ...
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bioRxiv - Microbiology 2021Quote: ... and 5 µl of the mixture was placed onto freshly glow discharged holey carbon grids (R 2/1, Quantifoil) for 1 min ...
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bioRxiv - Immunology 2021Quote: ... Two types of grids were used: UltrAuFoil R 1.2/1.3 (Au, 300-mesh; Quantifoil Micro Tools GmbH) and Quantifoil R 2/1 (Cu ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Microbiology 2021Quote: ... loaded onto a R2/2 200 mesh Cu grid (Quantifoil Micro Tools, GmbH), and plunge frozen in liquid ethane using the automated Leica EM GP plunge freezer (Leica Microsystems GmbH) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Samples (3 μL) were applied to a glow-discharged UltrAufoil R1.2/1.3 300 mesh holey grid (Quantifoil GmbH, Großlöbichau, Germany) and were flash frozen in liquid ethane using the Vitrobot mark IV (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... Aliquots of 1.25 – 1.5 μl RyR1-Nanodisc were applied onto each side of glow-discharged UltraAufoil −1.2/1.3 holey-gold (Quantifoil, Germany) with 300 TEM mesh ...
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bioRxiv - Plant Biology 2021Quote: 4 μl of GmQUAC1malate proteins (purified in 75 mM L-malate) were applied to newly glow-discharged holy carbon film grids (Au R1.2/1.3, 300 meshes, Quantifoil, Germany). The grids were blotted with force 3 and blotting time of 9.0 s at 100% humidity and 4°C and were vitrified by plunge freezing into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: Holey carbon grids (Quantifoil R2/1, 200 mesh) were plasma cleaned using a Solarus plasma cleaner (Gatan) ...
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bioRxiv - Microbiology 2021Quote: ... 3μl of protein solution at a concentration of 0.2 mg/ml were deposited on glow-discharged Quantifoil R 1.2/1.3 holey carbon grids (Quantifoil Micro Tools GmbH, Germany). Samples were manually blotted for four seconds at room temperature and vitrified by rapidly plunging into liquid ethane using a home-built plunging apparatus ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosome–EF-G complexes were applied to EM grids (Quantifoil 3.5/1 μm, Jena Bioscience) covered with pre-floated continuous carbon ...
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bioRxiv - Cell Biology 2021Quote: ... were frozen by applying a light cell suspension directly onto the front side of glow-discharged holey carbon grids (Q350AR-35, Quantifoil Micro Tools, Germany). Cells were pretreated with 5 mM LiCl to increase the length of flagella ...
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bioRxiv - Cell Biology 2021Quote: ... 3 µl of the samples at 1 µg/µl was applied to UltrAUfoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH). Grids were glow-discharged for 30 sec at 15 mA prior to sample application (PELCO easiGlow™ ...
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bioRxiv - Cell Biology 2021Quote: ... Quantifoil 300 mesh R1.2/1.3 (Quantifoil) grids were used and glow-discharged as above.
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bioRxiv - Cell Biology 2021Quote: ... 4 µl of the solution was applied to a glow-discharged (30s at 35 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools). After removing excess liquid by blotting the grid from the back side with Whatman filter paper for 2 s ...
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bioRxiv - Cell Biology 2021Quote: ... Aggregation was stimulated with TRAP-6 (25μM) in a 96-well plate (15 minutes, 750rpm, 37°C; BioShake IQ, Quantifoil Instruments) and fixed with 1% formalin (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... a thin layer of homemade continuous carbon was floated on top of individual Quantifoil R3.5/1 200 mesh gold grids (Quantifoil Micro Tools). After drying ...
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bioRxiv - Biophysics 2021Quote: ... 3.5 μL of the mixture was applied onto a glow-discharged Quantifoil R1.2/1.3 400 mesh Au grid (Quantifoil), blotted for 4 s at room temperature (RT ...
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bioRxiv - Biophysics 2021Quote: Purified HsTMEM120A at 5 mg/ml was applied to a glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grid (Quantifoil, Micro Tools GmbH, Germany), blotted under 100% humidity at 4°C and plunged into liquid ethane using a Mark IV Vitrobot (FEI).
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bioRxiv - Biophysics 2021Quote: ... Aliquots of 4 µl were applied to glow-discharged 300 mesh R1.2/1.3 Au grids (Quantifoil) using a Vitrobot Mark VI (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: Graphene oxide coating was performed on 1.2/1.3 holey patterned carbon or gold film grids (Protochips or Quantifoil). To wash them ...
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bioRxiv - Biophysics 2021Quote: The freshly purified AVP–V2R–Gs complex (3.0 μL) at a final concentration of 4.2 mg/mL was applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 300 mesh), and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... purchased from Quantifoil. All grids were glow discharged in air using GloQube glow discharge system (Quorum ...
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bioRxiv - Cell Biology 2021Quote: R 2/1 Holey Carbon Au 200 mesh EM grids (Quantifoil) were coated with approximately 20 nm of carbon in a MED 020 carbon coater (BAL-TEC ...
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bioRxiv - Biochemistry 2021Quote: ... The P5CS filament samples were placed on H2/O2 glow-discharged holey carbon grids (Quantifoil Cu 300 mesh, R1.2/1.3) or amorphous alloy film (No ...
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bioRxiv - Plant Biology 2021Quote: Concentrated PSII solution (3 µl) was applied on glow-discharged holey carbon grids (Cu Quantifoil R1.2/1.3) that were vitrified for cryo-EM structural determination using a Vitrobot FEI (3 s blot at 4°C and 100% humidity) ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μl of the resultant mixture was applied to glow-discharged (EasiGlow Pelco) R2/2 Quantifoil grids (Quantifoil). Excess sample was blotted away with a FEI Vitrobot (IV ...
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bioRxiv - Biochemistry 2021Quote: ... 4 μL sample (0.08-0.15 mg/ml) was then spotted on glow discharged Quantifoil holey grids (R2.2, Cu 300 mesh, Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2021Quote: ... Aliquot (2.5 μL) of the sample was placed onto a holey-carbon copper grid (Quantifoil Micro Tools, R 1.2/1.3) previously glow-discharged in a plasma ion bombarder (PIB-10 ...
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bioRxiv - Microbiology 2021Quote: ... 200-mesh copper grid with holey carbon film (R2/1 Cu 200, Quantifoil Micro Tools GmbH, Germany). The sample was blotted with filter paper and immediately plunge frozen in liquid ethane ...
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bioRxiv - Neuroscience 2021Quote: ... resuspended sarkosyl insoluble pellets were applied to glow-discharged holey carbon gold grids (Quantifoil R1.2/1.3, 300 mesh) and plunge-frozen in liquid ethane using an FEI Vitrobot Mark IV ...
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bioRxiv - Biochemistry 2021Quote: Samples (3µL) were applied to glow-discharged Quantifoil R1.2/1.3 Cu/Rh 200 mesh grids (Quantifoil GmbH, Großlöbichau, Germany) and were flash frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher Scientific ...