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Citations for Millipore Sigma :
4451 - 4500 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were mounted on glass microscopy slides with DAPI-containing mounting medium (4’,6-Diamidino-2-phenylindole dihydrochloride, Vectashield-H-1200-10, Sigma-Aldrich). All brain slices were imaged by a confocal microscope (LSM 710 ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampal slices were post-fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 24 h and transferred to 30% sucrose (Sigma-Aldrich). Slices were sectioned coronally at 20 μm and stored at −20 °C in cryoprotectant (20% glycerol ...
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bioRxiv - Developmental Biology 2023Quote: ... #3 and #4 were pooled and processed to obtain a single cell suspension through mechanical disaggregation followed by 2 h of enzymatic digestion in DMEM + 125 μg/mL collagenase (Sigma-Aldrich), 0.25 µg/mL dispase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Fully grown oocytes were harvested from female mice at 28–35 days of age that were superovulated 44 h later by injection of 5 IU of PMSG in 37 °C pre-warmed M2 medium (M7167; Sigma-Aldrich) and cultured in M16 medium (M7292 ...
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bioRxiv - Developmental Biology 2024Quote: Total mRNA was extracted from larval brains of control (yw) and mrtf[Delta7] at 24 h ALH using TRI Reagent (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were restimulated after 72 h of culture with PMA (50 ng ml-1) and ionomycin (1 µg ml-1, both from Sigma-Aldrich) in the presence of brefeldin A (5 µg ml-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... HelaS3 cells were transduced for 48 h in 6 well plate in 1 ml of medium containing 8 µg/mL of polybrene (Sigma-Aldrich). VHL-NbGFP4-FLAG HelaS3 cell line was obtained after selection with 1 µg/mL of puromycin (Invivogen).
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... A fraction of the cell lysates was used to estimate NAD(H) levels using the NAD/NADH quantification kit (Sigma MAK037) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... for 1 h at room temperature simultaneously with 4′,6-diamidino-2-phenylindole (DAPI: 1:2500, D9542; Sigma-Aldrich-Aldrich, USA) and Phalloidin Alexa488 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then cultured for 3 h in T cell stimulation media (1000ng/mL PMA [Sigma Aldrich, Catalog no. P1585-1MG], 2µM Ionomycin [Sigma Aldrich, Catalog no ...
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bioRxiv - Microbiology 2023Quote: ... 12 h post-transfection the media was removed and fresh media was added with 3 mM Sodium butyrate (Sigma-Aldrich, USA) to induce lentivirus replication ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.2% Triton X-100 diluted in PBS for 1 h before incubation with the following antibodies: mouse anti-GFAP (1:1,000; Millipore, Cat. MAB360), rabbit anti-LCN-2 (1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Stationary phase cultures (37°C, 18-24 h, with shaking) were washed twice with sterile phosphate buffered saline (PBS; D8537, Sigma-Aldrich) and diluted to 5 x 105 CFU.mL−1 for experiments ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated for 1 h in a 1:1 mixture of 100 % Ethanol and 100 % Epon 812 substitute (Epoxy embedding kit 45359, Sigma-Aldrich). After an overnight incubation with 100 % Epon in 1.5 mL Eppendorf tubes with open lids ...
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bioRxiv - Biochemistry 2023Quote: ... 50 µg H*-Cdc42:CTIS was mixed with 5 or 50 U of TEV protease (10 U/µL) (T4455, Sigma-Aldrich) in SEC buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mg/mL of streptomycin (P/S) (Cat# 4-01F00-H, BioConcept) and 10 % fetal bovine serum (FBS) (Cat# S0615, Sigma-Aldrich) at 37 °C in a humidified atmosphere with 5 % CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... permeabilized with 0.25%Triton X-100/PBS for 15 min and blocked for 1 h at RT with 5% NGS (Jackson Immuno Research, 005-000-121) + 5% BSA (Sigma, A2153). The following primary antibodies were used ...
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bioRxiv - Genomics 2022Quote: ... and 40 h later treated with 2 μg/mL doxycycline hyclate or seeded in 96-well plates at 3e3 cells per well and 24 h later treated with 1 μg/mL doxycycline hyclate and either DMSO or 10 μM MG132 (Sigma-Aldrich). Caspase 3/7 activity was measured 16 and 24 h later using the Caspase-Glo 3/7 Assay System (Promega ...
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bioRxiv - Microbiology 2022Quote: ... at 50 μM and 100 μM concentrations while H+-dependent motility was inhibited using the protonophore Carbonyl cyanide 3-chlorophenylhydrazone (CCCP, C2759 Sigma-Aldritch).
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg dsRNA were added and incubated for 1 h at 27°C, before adding 1 ml full medium (ExpressFive SFM Invitrogen, 16 mM Glutamine, 10% FBS (Sigma-Aldrich), 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2022Quote: ... samples were incubated for 1 h with 1 μg/ml DAPI and 50 pmol Atto 550-conjugated Phalloidin (19083, Sigma-Aldrich). Samples were washed and imaged in PBS images were acquired with a confocal laser scanning microscope (Leica TCS SP8X ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting circle DNA can be amplified (1 h 40 min amplification at 37 °C, Duolink In Situ Detection Reagents Green, Sigma-Aldrich) and visualized by fluorescently labeled complementary oligonucleotide probes incorporation ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were then incubated for 1 h at 37 °C with secondary antibodies each harboring a PLA probe (Duolink In Situ PLA MINUS/PLUS probes, Sigma-Aldrich). The PLA probes that bind to the constant regions of the primary antibodies contain a unique DNA strand ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with 2 mM thymidine for 24 h followed by release from thymidine into fresh medium containing 100 ng/ml nocodazole (Sigma, M1404) for 18 h ...
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bioRxiv - Immunology 2022Quote: ... THP-1 monocytes are differentiated into macrophages by 36 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139). The murine macrophage Raw 274.7 cells (ATCC ...
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bioRxiv - Immunology 2022Quote: ... for 1 h and for inhibition of stress granule formation cells were treated with 1.5 µg/ml of ISRIB (Sigma-Aldrich, SML0843). Stable HeLa cell lines constitutively expressing human marker proteins for membraneless compartments ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and incubated for 4 h at 4 °C on a rotating wheel with 40 μl of packed anti-FLAG M2 affinity gel (Sigma, #A2220), which had been washed three times with lysis buffer prior to use ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... heparin synchronised (4 h invasion window) trophozoite stage parasites were treated for one cell cycle (trophozoite to trophozoite) with GlcN (Sigma-Aldrich) and parasite pellet extensively washed in PBS containing protease inhibitor cocktail (PI ...
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bioRxiv - Molecular Biology 2022Quote: ... hCAI with the K978C mutation (h^K978C) or for T2A mutagenesis were designed using SnapGene software (SI, Table 7) and synthesised by Sigma Aldrich.
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bioRxiv - Systems Biology 2022Quote: ... The tip of the silanized pipettes was filled with a H+-selective ionophore cocktail (hydrogen ionophore I cocktail A, Sigma-Aldrich) by dipping the tip of the pipette into the solution ...
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bioRxiv - Genomics 2022Quote: ... K562 or Jurkat cell lines expressing CRISPRi effectors of interest were infected with lentivirus containing sgRNA expression vectors by centrifugation at 1000 × g for 1 h in 24-well plates in the presence of 8 µg/mL polybrene (Sigma-Aldrich). RPE1 ...
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bioRxiv - Biochemistry 2023Quote: ... concentration of the proteins in buffer H containing 500 mM NaCl was diluted to 150 mM and the samples were supplemented with 4% Ficoll PM70 (Sigma-Aldrich). Then the samples were incubated in a glass-based 384-well plate (EZVIEW assay plate ...
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bioRxiv - Bioengineering 2022Quote: ... A quantitative evaluation was done after 15 h by a dosage of lysosomal activity (p-nitrophenyl-n-acetyl-betaD-glucosamide, Sigma-Aldrich); and iv ...
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bioRxiv - Cell Biology 2023Quote: ... infiltrated and embedded in Durcupan (25%, 50%, 75% Durcupan in acetone for 1 h each and 100% Durcupan overnight; 44610, Sigma, Germany) to get polymerized for 48 h at 60°C ...
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bioRxiv - Cell Biology 2022Quote: ... cells were harvested after 6 h in sporulation medium (SPM) and resuspended in SPM containing 120 µg/ml benomyl or 0.4% DMSO (Sigma #D8418, USA) for 2 h before they were analyzed for protein localization ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were cultured for 72 h on a collagen-ECM-coating ((0.05 mg/ml collagen type I (Sigma-Aldrich: A1048301), 0.5 mg/ml protein of ECM gel (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were cultured for 72 h on a collagen-ECM-coating ((0.05 mg/ml collagen type I (Sigma-Aldrich: A1048301), 0.5 mg/ml protein of ECM gel (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 mg of the whole cell extract was incubated for 2 h at 4°C with 15 μl of M2-anti Flag agarose (Sigma- Aldrich), GFP-trap agarose (Chromo Tek ...
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bioRxiv - Cell Biology 2023Quote: ... elegans ChIP reaction or from 50% of a control extract (1 mg protein) were de-crosslinked at 65 °C for at least 4 h with 150 μg/ml Proteinase K (Sigma, #3115887001). DNA from each ChIP reaction or from control extract was isolated using the Qiagen PCR purification kit and diluted to a final volume of 200 μl with (10 mM Tris-HCl ...
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bioRxiv - Genetics 2023Quote: ... at 4°C for 1 h followed by overnight incubation with 3 μL of the following antibodies: H3K4me2 (Millipore 07-030), H3K9me3 (Active Motif 39161) ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained by incubation at room temperature for at least 2 h with a primary antibody in perm/block buffer (0.5% saponin (Sigma-Aldrich, USA), 1% BSA) ...
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bioRxiv - Microbiology 2023Quote: ... serial dilutions of monoclonal antibodies in duplicate were prepared in a 96-well microtiter plate and pre-incubated with pseudovirus for 1 h at 37 °C in the presence of a final concentration of 5 mg/mL polybrene (EMD Millipore), before the pseudovirus-mAb mixtures were added to 293T-hACE2 monolayers ...
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bioRxiv - Cancer Biology 2022Quote: Primary tumor samples and lungs were fixed in 4% paraformaldehyde (PFA) at 4°C for 24 h and decalcified in 14% EDTA (Sigma-Aldrich) for up to 28 d at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at least 12 larvae were pooled together per sample in 1.5 ml tube and incubated for 1 h at room temperature in 200 µl of the extraction buffer: 4% IGEPAL (Millipore, Sigma) in 100% isopropanol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... This was followed by a 1 h incubation at room temperature with anti-mouse or anti-rabbit secondary antibodies conjugated with horseradish peroxidase (HRP; Sigma, A8924). Blots were developed using a Konica SRX101A X-Ray Film Processor.
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bioRxiv - Bioengineering 2023Quote: ... 0.1% Tween 20) and incubated for 1 h at room temperature or overnight at 4°C with primary solution (anti-FLAG (Sigma F1804), diluted 1:1000 in 5% milk in TBST) ...
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bioRxiv - Genetics 2023Quote: ... The membrane was blocked with 5% nonfat dry milk in Tris-buffered saline for 1 h and incubated with primary antibodies for HTT (MAB2166, Sigma-Aldrich) for 12 h at 4 °C ...